Previous studies clearly indicated that membrane enzymes - ATPases i.e. Na, K-ATPase and Mg-ATPase, responded to presence of various organic and inorganic pollutants. In this work effect of mercury and cadmium on these enzymes activities was investigated in synaptic plasma membranes partially immobilized on microliter plate. Comparing those activities with the control enzyme activities obtained with native and partially immobilized mambranes it was concluded that: a. both metals exerted a concentration-dependent inhibition of investigated enzymes, b. for partially immobilized membranes estimated half maximum inhibition (IC50) values for Na, K-ATPase were IC50 (Hg) = 0.9 micromol/cubic cm, IC50 (cd) = 35 micromol/cubic cm and for Mg - ATPase IC50 (Hg) = 3.5 micromol/cubic cm, IC50 (Cd) = 36 micromol/cubic cm; for native membranes IC50 for Na,K-ATPase were IC50 (Hg) =3.3 micromol/cubic cm, IC50 (Cd) = 2 micromol/cubic cm and for Mg ATPase IC50 (Hg) = 2.3 micromol/cubic cm, IC50 (Cd) = 0.2 mmol/cubic cm. Obtained results indicate avaibility of microtitar plates for partially immobilization of membranes with aim to form a new biosensor for heavy metals detection.

The reaction of Pd(II) with 1,8-dihydroxy-2-(pyrazol-5-ylazo)-naphthalen-3,6-disulphonic acid immobilized by physical sorption onto Dowex 1-X8 ion-exchange resin was investigated with the aim to develop the sorption-spectroscopic test method for the detection of low Pd(II) concentrations in water. The resin phase absorption spectra of the reagent and its Pd(II) complex were followed. The immobilized reagent has the spectral characteristics similar to those in the water and forms with Pd(II) 1:1 complex with the absorption maximum at 650 nm. Parameters, such as pH, wavelength and contact time have been optimized for a given amount of the sorbed reagent. The experimental conditions for the linear dependence of absorbance vs. Pd(II) concentration have been determined.

Water bodies like rivers and lakes are directly affected by the waste water in sewerage systems. Sewage systems provide waste collection and convey wastewater from the population in sanitary safely way and away from material property. DPSIR analytical framework allows the analysis of interactions between sewerage systems and water bodies. Sewer systems describe the different standards that are elements of their functioning including plants and their maintenance, hydrological, hydraulic and other influences. This paper gives analytical framework of Driver, Pressure, State, Impact, Response (DPSIR) in relation to the functioning of the elements considered sewage systems which are described in the Serbian (European) standard - SRPS EN 752 - 2:2007.

The paper presents a comparison of measurements of selected organic pollutants in various water matrices, in order to assess the impact of the matrix on the accuracy of pollutant analysis. Selected compounds were from class of phenols (p-nitrophenol, 3,5-dichlorophenol)and organochlorine pesticides (isomers of hexachlorocyclohexane (HCH), heptachlor, aldrine, heptahloroepoxide, 4,4'-DDE, dieldrine, endrine, 4,4'-DDD, endrin aldehyde, 4,4'-DDT, endosulfan sulfate). Apart from distilled water and synthetic aqueous matrix (salt addition in distilled water according to DIN EN 12902 standard), natural matrix was also used in the analysis – surface water (River Danube in Novi Sad – DOC (dissolved organic carbon) 2-6 mg/l, suspended matter content 30 mg/l).

The 1st South-Eastern European Interlaboratory Study: WATER ANALYSIS - 2003 was organized by International Scientific Committee on the basis of international European and regional experiences. The total of 38 laboratories took part in WATER-ANALYSIS 2003. Three laboratories were from Greece, two from the Republic of Srpska (Bosnia and Herzegovina), eight from Romania and all others from Serbia and Montenegro. Two samples were prepared from water of the river Danube and the task for participants was to determine trace elements: Al, As, Cd, Cu, Mn, Fe, Pb, and Zn. Results of determination of arsenic (As) and aluminium (Al) are presented in this paper.

Potentiometric stripping analysis (PSA) is highly sensitive electroanalytical method for determination of traces of heavy metals. In this paper detection limit and linearity of potentiometric stripping analysis for lead was investigated. Method was successfully used for determination of traces of lead ion (detection limit 5 microgram/cubic dm) in stream water of river Ibar, before and after joint of potential pollutant.

The aim of this study was to determine the content of heavy metals, cadmium and lead, in drinking water, spring and well water from rural part of south Serbia, as in water of river Jablanica and in the top water. The content of cadmium and lead, a very toxic metals, were studied simulataneously by potentiometric stripping analysis (PSA) in water samples using thin mercury film electrode, as a working electrode, by standard addition method in the presence of supporting electrolyte. The results of content of examined heavy metals obtained in examined water samples by PSA method, are compared with results obtained by using AAS and GF-AAS spectrometry.

Organic matter of various composition is present in soils and sediments ranging from simple compounds to the very complex proteins, etc. Within the pollutant analysis in soils and sediments the total organic carbon (TOC) content is determined. In addition to this form carbon may be present as elemental or inorganic ones. TOS identification and quantitation methods may be qualitative or quantitative with prior sample preparation. It is recommended catalytic combustion of samples at high temperatures with Pt catalyst and IR detection of evolved CO2.