Leaf-cutting ants are important pests of forests and agricultural crops in the Neotropical region. Atta sexdens colonies can be composed of thousands of individuals, which form a highly complex society with a single reproductive queen. Successful control of this species is achieved only if the queen is affected. Few data are available on the lethal or sublethal effects of toxic compounds on leaf-cutting ant queens. Azadirachtin has been claimed as an effective biopesticide for insect control, but its action on leaf-cutting ants has been little explored. This study shows that azadirachtin affects oviposition in A. sexdens queens, impairing egg development by decreasing protein reserves. Azadirachtin inhibits the synthesis of vitellogenin, the major yolk protein precursor. The negative effects of azadirachtin on the reproduction of leaf-cutting ant queens suggest a potential use for the control of these insects.

This study aims to determine the maximum concentration and the long-term effects after exposure to a bio-insecticide with active ingredients eugenol and azadirachtin on the survival rate, immunity, and growth of Nile tilapia. The method used in this study was experimental, using a completely randomized design (CRD) with six treatments and three replications. Fishes were exposed to eugenol and azadirachtin at concentrations 10, 20, 30, 40, and 50% of LC50 value for 14 days, followed by 14 days of maintenance to see the effect on growth. The results showed that 66 mg.L-1 treatment was a concentration that did not interfere with the survival rate of Nile tilapia, which was 86.7%. The number of leukocytes increased on the third day by the highest increase in 66 mg.L-1 treatment at 12.01 × 104 cells.mm-3. Meanwhile, erythrocytes decreased, with the highest decrease in 66 mg.L-1 treatment at 1.13 × 106 cells.mm-3. The average growth rate in fish slowed down with increasing concentrations of exposure, with the lowest average growth in length and absolute weight in the 66 mg.L-1 treatment was 0.57 cm and 1.68 g.

This study aims to determine the maximum concentration and the long-term effects after exposure to a bio-insecticide with active ingredients eugenol and azadirachtin on the survival rate, immunity, and growth of Nile tilapia. The method used in this study was experimental, using a completely randomized design (CRD) with six treatments and three replications. Fishes were exposed to eugenol and azadirachtin at concentrations 10, 20, 30, 40, and 50% of LC50 value for 14 days, followed by 14 days of maintenance to see the effect on growth. The results showed that 66 mg.L-1 treatment was a concentration that did not interfere with the survival rate of Nile tilapia, which was 86.7%. The number of leukocytes increased on the third day by the highest increase in 66 mg.L-1 treatment at 12.01 × 104 cells.mm-3. Meanwhile, erythrocytes decreased, with the highest decrease in 66 mg.L-1 treatment at 1.13 × 106 cells.mm-3. The average growth rate in fish slowed down with increasing concentrations of exposure, with the lowest average growth in length and absolute weight in the 66 mg.L-1 treatment was 0.57 cm and 1.68 g.

India is one of the leading countries in production and indiscriminate consumption of pesticides. Owing to their xenobiotic nature, pesticides affect soil microorganisms that serve as mediators in plant growth promotion. Our study aimed to deliver a comprehensive picture, by comparing the effects of synthetic pesticides (chlorpyriphos, cypermethrin, and a combination of both) with a biopesticide (azadirachtin) at their recommended field application level (L), and three times the recommended dosage (H) on structure and function of microbial community in rhizosphere of Vigna radiata. Effect on culturable fraction was assessed by enumeration on selective media, while PCR-denaturing gradient gel electrophoresis (DGGE) was employed to capture total bacterial community diversity. This was followed by a metabolic sketch using community-level physiological profiling (CLPP), to obtain a broader picture of the non-target effects on rhizospheric microbial community. Although plant parameters were not significantly affected by pesticide application, the microbial community structure experienced an undesirable impact as compared to control devoid of pesticide treatment. Examination of DGGE banding patterns through cluster analysis revealed that microbial community structure of pesticide-treated soils had only 70% resemblance to control rhizospheric soil even at 45 days post application. Drastic changes in the metabolic profiles of pesticide-treated soils were also detected in terms of substrate utilization, rhizospheric diversity, and evenness. It is noteworthy that the effects exacerbated by biopesticide were comparable to that of synthetic pesticides, thus emphasizing the significance of ecotoxicological assessments before tagging biopesticides as “safe alternatives.”

Intensive agriculture has resulted in an indiscriminate use of pesticides, which demands in-depth analysis of their impact on indigenous rhizospheric microbial community structure and function. Hence, the objective of the present work was to study the impact of two chemical pesticides (chlorpyrifos and cypermethrin) and one biological pesticide (azadirachtin) at two dosages on the microbial community structure using cultivation-dependent approach and on rhizospheric bacterial communities involved in nitrogen cycle in Vigna radiata rhizosphere through cultivation-independent technique of real-time PCR. Cultivation-dependent study highlighted the adverse effects of both chemical pesticide and biopesticide on rhizospheric bacterial and fungal communities at different plant growth stages. Also, an adverse effect on number of genes and transcripts of nifH (nitrogen fixation); amoA (nitrification); and narG, nirK, and nirS (denitrification) was observed. The results from the present study highlighted two points, firstly that nontarget effects of pesticides are significantly detrimental to soil microflora, and despite being of biological origin, azadirachtin exerted negative impact on rhizospheric microbial community of V. radiata behaving similar to chemical pesticides. Hence, such nontarget effects of chemical pesticide and biopesticide in plants’ rhizosphere, which bring out the larger picture in terms of their ecotoxicological effect, demand a proper risk assessment before application of pesticides as agricultural amendments.