Polycyclic aromatic hydrocarbons (PAHs) are hazardous toxic contaminants and considered as primary pollutants due to their persistent nature and most of them are carcinogenic and mutagenic. The key challenge in PAHs degradation is their hydrophobic nature, which makes them one of the most complex materials and inaccessible by a broad range of microorganisms. This bioavailability can be increased by using a biosurfactant. In the present study mixed PAHs were degraded using the biosurfactant producing bacterial strains. In addition, iron nanoparticles were synthesized and the impact of iron nanoparticles on the growth of the mixed bacterial strains (Pseudomonas stutzeri NA3 and Acinetobacter baumannii MN3) was optimized. The mixed PAHs (anthracene, pyrene, and benzo(a)pyrene) degradation was enhanced by addition of biosurfactant (produced by Bacillus subtilis A1) and iron nanoparticles, resulting in 85% of degradation efficiency. The addition of the biosurfactant increased the bioavailability of the PAHs in the aqueous environment, which might help bacterial cells for the initial settlement and development. The addition of iron nanoparticles increased both bacterial biomass and PAHs adsorption over their surface. These overall interactions assisted in the utilization of PAHs by the mixed bacterial consortia. This study illustrates that this integrated approach can be elaborated for the removal of the complex PAHs pollutants from soil and aqueous environments.

Discharged carbon nanotubes (CNTs) likely interact with co-existing organic contaminants (OCs) and pose joint toxicity to environmental microbes. Herein, hydrophobic pentachlorophenol (PCP) and hydrophilic ciprofloxacin (CIP) were used as representative OCs and their joint toxicities with CNTs to Bacillus subtilis were systematically investigated at cellular, biochemical, and omics levels. The 3-h bacterial growth half inhibitory concentrations of CNTs, PCP, and CIP were 12.5 ± 2.6, 3.5 ± 0.5, and 0.46 ± 0.03 mg/L, respectively, and they all could damage cell membrane, increase intracellular oxidative stress, and alter bacterial metabolomics and transcriptomics; while CNTs-PCP and CNTs-CIP binary exposures exhibited distinct additive and synergistic toxicities, respectively. CNTs increased bacterial bioaccumulation of PCP and CIP via destabilizing and damaging cell membrane. PCP reduced the bioaccumulation of CNTs, while CIP had no significant effect; this difference could be owing to the different effects of the two OCs on cell-surface hydrophobicity and CNTs electronegativity. The additive toxicity outcome upon CNTs-PCP co-exposure could be a result of the balance between the increased toxicity from increased PCP bioaccumulation and the decreased toxicity from decreased CNTs bioaccumulation. The increased bioaccumulation of CIP contributed to the synergistic toxicity upon CNTs-CIP co-exposure, as confirmed by the increased inhibition of topoisomerase Ⅳ activity and interference in gene expressions regulating ABC transporters and lysine biosynthesis. The findings provide novel insights into environmental risks of CNTs.

The high density and viscosity of fuel oil leads to its prolonged persistence in the environment and causes widespread contamination. Dispersants with a low environmental impact are necessary for fuel oil spill remediation. This study aimed to formulate bio-based dispersants by mixing anionic biosurfactant (lipopeptides from Bacillus subtilis GY19) with nonionic oleochemical surfactant (Dehydol LS7TH). The synergistic effect of the anionic-nonionic surfactant mixture produced a Winsor Type III microemulsion, which promoted petroleum mobilization. The hydrophilic-lipophilic deviation (HLD) equations for ionic and nonionic surfactant mixtures were compared, and it was found that the ionic equation was applicable for the calculation of lipopeptides and Dehydol LS7TH concentrations. The best formula contained 6.6% w/v lipopeptides and 11.9% w/v Dehydol LS7TH in seawater, and its dispersion effectiveness for bunker fuels A and C was 92% and 78%, respectively. The application of bio-based dispersants in water sources was optimized by Box-Behnken design. The efficiency of the bio-based dispersant was affected by the dispersant-to-oil ratios (DORs) but not by the water salinity. A suitable range of DORs for different oil contamination levels could be identified from the response surface plot. The dispersed fuel oil was further degraded by adding an oil-degrading bacterial consortium to the chemically enhanced water accommodated fractions (CEWAFs). After 7 days of incubation, the concentration of fuel oil was reduced from 3692 mg/L to 356 mg/L (88% removal efficiency). On the other hand, the abiotic control removed less than 40% fuel oil from the CEWAFs. This bio-based dispersant had an efficiency comparable to that of a commercial dispersant. The process of dispersant formulation and optimization could be applied to other surfactant mixtures.

The primary objective of this study was to identify the capacity and mechanism of extracellular polymeric substance (EPS) adsorption on soil colloids of Alfisol and Ultisol at different pH and ionic strengths. Two kinds of EPS were extracted from Bacillus subtilis and Pseudomonas fluorescens by centrifugation, and their adsorption on Ultisol and Alfisol was investigated using a batch adsorption experiment and attenuated total reflectance–Fourier transform infrared spectroscopy (ATR-FTIR). The average diameter of EPS from B. subtilis and P. fluorescens was 1825 and 1288 nm, respectively, and both the EPS were negatively charged. The zeta potentials of the two EPS became more negative with increasing solution pH from 3 to 8 and less negative with increasing ionic strength from 0 to 80 mM. The maximum adsorption capacity of EPS-C and EPS-N on Alfisol was higher than that on Ultisol, whereas the maximum adsorption capacity of EPS-P on Alfisol was lower than that on Ultisol. The adsorption of EPS-C, EPS-N, and EPS-P of both the EPS on Ultisol and Alfisol decreased with increasing solution pH from 3 to 8. Adsorption of EPS-C, EPS-N, and EPS-P of both the EPS on Alfisol significantly increased with increasing ionic strength from 0 to 10 mM, whereas it remained constant, slightly increased, or reduced, when the ionic strength was increased from 10 to 80 mM. The adsorption of EPS-C, EPS-N, and EPS-P on Ultisol slightly increased with increasing ionic strength from 0 to 80 mM. Saturation coverage determined by ATR-FTIR showed that adsorption of whole EPS on Ultisol was higher than that on Alfisol at pH 6 after 60 min. Thus, electrostatic force between EPS and soil colloids played an important role in EPS adsorption. Besides, proteins and phosphate groups in EPS also contributed to EPS adsorption on soil colloids.

Extracellular polymeric substances (EPS) isolated from bacteria, are abound of functional groups which can react with metals and consequently influence the immobilization of metals. In this study, we combined with Zn K-edge Extended X-ray Absorption Fine Structure (EXAFS), Fourier Transform Infrared (FTIR) spectroscopy, and High-Resolution Transmission Electron Microscopy (HRTEM) techniques to study the effects of EPS isolated from Bacillus subtilis and Pseudomonas putida on Zn sorption on γ-alumina. The results revealed that Zn sorption on aluminum oxide was pH-dependent and significantly influenced by bacterial EPS. At pH 7.5, Zn sorbed on γ-alumina was in the form of Zn-Al layered doubled hydroxide (LDH) precipitates, whereas at pH 5.5, Zn sorbed on γ-alumina was as a Zn-Al bidentate mononuclear surface complex. The amount of sorbed Zn at pH 7.5 was 1.3–3.7 times higher than that at pH 5.5. However, in the presence of 2 g L−1 EPS, regardless of pH conditions and EPS source, Zn + EPS + γ-alumina ternary complex was formed on the surface of γ-alumina, which resulted in decreased Zn sorption (reduced by 8.4–67.8%) at pH 7.5 and enhanced Zn sorption (increased by 10.0–124.7%) at pH 5.5. The FTIR and EXAFS spectra demonstrated that both the carboxyl and phosphoryl moieties of EPS were crucial in this process. These findings highlight EPS effects on Zn interacts with γ-alumina.

We investigated the effect of increasing CO₂ concentrations on the growth and viability of ecophysiologically different microorganisms to obtain information for a leakage scenario of CO₂ into shallow aquifers related to the capture and storage of CO₂ in deep geological sections. CO₂ concentrations in the gas phase varied between atmospheric conditions and 80% CO₂ for the aerobic strains Pseudomonas putida F1 and Bacillus subtilis 168 and up to 100% CO₂ for the anaerobic strains Thauera aromatica K172 and Desulfovibrio vulgaris Hildenborough. Increased CO₂ concentrations caused prolonged lag-phases, and reduced growth rates and cell yields; the extent of this effect was proportional to the CO₂ concentration. Additional experiments with increasing CO₂ concentrations and increasing pressure (1–5000 kPa) simulated situations occurring in deep CO₂ storage sites. Living cell numbers decreased significantly within 24 h at pressures ≥1000 kPa, demonstrating a severe lethal effect for the combination of high pressure and CO₂.

Toxicity of nano-scaled aluminum, silicon, titanium and zinc oxides to bacteria (Bacillus subtilis, Escherichia coli and Pseudomonas fluorescens) was examined and compared to that of their respective bulk (micro-scaled) counterparts. All nanoparticles but titanium oxide showed higher toxicity (at 20 mg/L) than their bulk counterparts. Toxicity of released metal ions was differentiated from that of the oxide particles. ZnO was the most toxic among the three nanoparticles, causing 100% mortality to the three tested bacteria. Al2O3 nanoparticles had a mortality rate of 57% to B. subtilis, 36% to E. coli, and 70% to P. fuorescens. SiO2 nanoparticles killed 40% of B. subtilis, 58% of E. coli, and 70% of P. fluorescens. TEM images showed attachment of nanoparticles to the bacteria, suggesting that the toxicity was affected by bacterial attachment. Bacterial responses to nanoparticles were different from their bulk counterparts; hence nanoparticle toxicity mechanisms need to be studied thoroughly. Oxide nanoparticles show higher toxicity than their bulk counterparts.

This work aimed to study the performance of paraquat removal by cell-immobilized ceramics. Two strains of paraquat degrading bacteria, Pseudomonas putida and Bacillus subtilis, were separately immobilized on the ceramic with and without wastewater sludge addition. Results showed that the ceramic surface with sludge has more functional groups and a more highly negative charge on the surface than the original ceramic. The ceramic with sludge had 2-3-fold of the immobilized cells higher than that of the control (without sludge) and less leaching of the immobilized cells. The sludge addition at 20% (w/w) to the ceramic provided the highest cell adhesion for both P. putida and B. subtilis. The paraquat removal efficiencies were higher than 98%, while the control ceramic could remove only 77 ± 1.2%. The immobilized cells on ceramic with sludge provided a significant degree of dissolved organic nitrogen reduction (82%) during the paraquat removal. Most organic nitrogen in paraquat was biologically mineralized (ammonified). Findings from this work suggest the superiority of ceramic with sludge in mineralizing organic nitrogen associated with paraquat.

Ion sorption on soil and sediment has been reported to be potentially affected by bacteria which may interact both physically and chemically with solid surfaces. However, whether and how bacteria affect the sorption of inorganic phosphate (P) on soil colloids remains poorly known. Here, we comparably investigated the P sorption on four soil colloids (three highly weathered soils including two Oxisols and one Ultisol and one weakly weathered soil Alfisol) and their complexes with Bacillus subtilis and Pseudomonas fluorescens. Batch experiments showed a notable reduction in P sorption on the colloids of highly weathered soils by the two bacteria at varying P concentrations and pHs; whereas that on the colloids of Alfisol appeared to be unaffected by the bacteria. The inhibitory effect was confirmed by both greater decline in P sorption at higher bacteria dosages and the ability of the bacteria to desorb P pre-adsorbed on the colloids. Further evidence was given by isothermal titration calorimetric experiments which revealed an alteration in enthalpy change caused by the bacteria for P sorption on Oxisol but not for that on Alfisol. The B. subtilis was more efficient in suppressing P sorption than the P. fluorescens, indicating a dependence of the inhibition on bacterium type. After association with bacteria, zeta potentials of the soil colloids decreased considerably. The decrease positively correlated with the decline in P sorption, regardless of soil and bacterium types, demonstrating that the increment in negative charges of soil colloids by bacteria probably contributed to the inhibition. In addition, scanning electron microscopic observation and the Derjaguin–Landau–Verwey–Overbeek theory prediction suggested appreciable physical and chemical interactions between the bacteria and the highly weathered soil colloids, which might be another contributor to the inhibition. These findings expand our understandings on how bacteria mobilize legacy P in soils and sediments.

Hydrocarbons and their derivative compounds are recalcitrant in nature and causing adverse impacts to the environment and are classified as important pollutants. Removal of these pollutants from the atmosphere is a challenging process. Hydrophobic organic pollutants (HOPs) including crude oil, diesel, dotriacontane (C₃₂), and tetracontane (C₄₀) are subjected to the biodegradation study by using a bacterial consortium consist of Bacillus subtilis, Pseudomonas stutzeri, and Acinetobacter baumannii. The impact of pH and temperature on the biodegradation process was monitored. During the HOPs biodegradation, the impact of hydrocarbon-degrading extracellular enzymes such as alcohol dehydrogenase, alkane hydroxylase, and lipase was examined, and found average activity about 47.2, 44.3, and 51.8 μmol/mg⁻¹, respectively. Additionally, other enzymes such as catechol 1,2 dioxygenase and catechol 2,3 dioxygenase were found as 118 and 112 μmol/mg⁻¹ Enzyme as an average range in all the HOPs degradation, respectively. Also, the impact of the extracellular polymeric substance and proteins were elucidated during the biodegradation of HOPs with the average range of 116.90, 54.98 mg/L⁻¹ respectively. The impact of biosurfactants on the degradation of different types of HOPs is elucidated. Very slight changes in the pH were also noticed during the biodegradation study. Biodegradation efficiency was calculated as 90, 84, 76, and 72% for crude oil, diesel, C₃₂, and C₄₀, respectively. Changes in the major functional groups (CH, C–O–C, CO, =CH₂, CH₂, CH₃) were confirmed by FTIR analysis and intermediated metabolites were identified by GCMS analysis. The surface-active molecules along with the enzymes played a crucial role in the biodegradation process.