In this study, a strain of lactic acid bacteria Streptococcus salivarius was studied for its capacity to remove hexavalent chromium (Cr (VI)) from a liquid medium and to form biofilm. Both properties are useful for using the strain in bioremediation of metal-contaminated effluents. For biofilm formation capacity, three methods were used: the tube method (TM), the Congo red agar method (CRA) and adherence to polystyrene tissue culture plate method (TCP). S. salivarius, showed a positive-biofilm and a correlation between the three methods was noted. The bacterial surface hydrophobicity was studied using the microbial adhesion to solvents method (MATS). On AISI-316 L stainless steel, the strain with a hydrophobic surface showed a good adhesion on this support after 18 h incubation. The colonization of the supports and the biofilms formation by the bacterial cell was observed using scanning electron microscopy (SEM). The minimum inhibitory concentration (MIC) of Cr(VI) on S. salivarius was determined on MRS broth, it was relatively high and equal to 400mg/l. In addition, it displayed a remarkable capacity to reduce Cr(VI) concentration on the liquid medium containing initially 50 mg/l of Cr(VI) ; the percent removal rate was equal to approximately 42% after 72 h of incubation at 37 °C. In addition to its GRAS status, the obtained results suggested that S. salivarius could be successfully used in Cr(VI) bioremediation.