Over the last few decades, Anzali wetland has been at risk of pollutants, especially from heavy metals. The present research analyzes some physical and chemical properties and heavy metals concentration in 27 points in nine stations of Anzali wetland. The samples of each station have been mixed, acidic digested, and analyzed by inductively coupled plasma mass spectrometry (ICP-MS). The mean concentrations of Fe, Mn, Pb, Zn, As, Cd, Cu, Hg, Ni, and Cr have been 50527.2, 1210, 23.3, 79.4, 8.8, 0.23, 32.1, 0.25, 31.6, and 31.5 mg/Kg, respectively. In addition, it can be seen that the concentrations of Cd (0.31 mg/Kg) and As (25.47 mg/Kg) in Hendekhale station, Hg (0.52 mg/Kg) in Pirbazar station, and Pb (52.69 mg/Kg) in Khazar villa station surpass world surface rock average. According to contamination factor (CF), in case of Mn and Pb, both Hendekhale, and Khazar Villa stations have been in considerable contamination level. The calculation of Pollution Load Index (PLI) shows that Hendkhaleh and Khazar Villa stations have had moderate pollution. The mCd index survey indicates that only in Hendekhale station, has heavy metals contamination been at a low degree level of contamination. According to PER index, mercury metal contamination in Hendekhale station faces medium risk. The calculated mean ERM quotient indicates the probability of heavy metals toxicity, equal to 21% in the examined stations.