Understanding the composition and assembly mechanism of waterborne pathogen is essential for preventing the pathogenic infection and protecting the human health. Here, based on 16S rRNA sequencing, we investigated the composition and spatial variation of potentially pathogenic bacteria from different sections of the Pearl River, the most important source of water for human in Southern China. The results showed that the potential pathogen communities consisted of 6 phyla and 64 genera, covering 11 categories of potential pathogens mainly involving animal parasites or symbionts (AniP), human pathogens all (HumPA), and intracellular parasites (IntCelP). Proteobacteria (75.87%) and Chlamydiae (20.56%) were dominant at the phylum level, and Acinetobacter (35.01%) and Roseomonas (8.24%) were dominant at the genus level. Multivariate analysis showed that the potential pathogenic bacterial community was significantly different among the four sections in the Pearl River. Both physicochemical factors (e.g., NO₃–N, and suspended solids) and land use (e.g., urban land and forest) significantly shaped the pathogen community structure. However, spatial effects contributed more to the variation of pathogen community based on variation partitioning and path analysis. Null model based normalized stochasticity ratio analysis further indicated that the stochastic process rather than deterministic process dominated the assembly mechanisms by controlling the spatial patterns of potential pathogens. In conclusion, high-throughput sequencing shows great potential for monitoring the potential pathogens, and provided more comprehensive information on the potentially pathogenic community. Our study highlighted the importance of considering the influences of dispersal-related processes in future risk assessments for the prevention and control of pathogenic bacteria.

Microbial mercury (Hg) methylation transforms inorganic mercury to neurotoxic methylmercury (MeHg) mainly in aquatic anoxic environments. Sampling challenges in marine ecosystems, particularly in submarine canyons, leads to a lack of knowledge about the Hg methylating microbia in marine sediments. A previous study showed an enrichment of mercury species in sediments from the Capbreton Canyon where both geochemical parameters and microbial activities constrained the net MeHg production. In order to characterize Hg-methylating microbial communities from coastal to deeper sediments, we analysed the diversity of microorganisms’ (16S rDNA-based sequencing) and Hg methylators (hgcA based cloning and sequencing). Both, 16S rDNA and hgcA gene analysis demonstrated that the putative Hg-methylating prokaryotes were likely within the Deltaproteobacteria, dominated by sulfur-compounds based reducing bacteria (mainly sulfate reducers). Additionally, others clades were also identified as carrying HgcA gene, such as, Chloroflexi, Spirochaetes, Elusimicrobia, PVC superphylum (Plantomycetes, Verrucomicrobia and Chlamydiae) and Euryarchaea. Nevertheless, 61% of the hgcA sequences were not assigned to specific clade, indicating that further studies are needed to understand the implication of new microorganisms carrying hgcA in the Hg methylation in marine environments. These first results suggest that sulfur cycle drives the Hg-methylation in marine ecosystem.

Atrazine, a common chemical pesticide, has toxicity to adult and juvenile amphibians in natural ecosystems; however, it is more common to study its effects on larvae instead of adults. This study assessed the impacts of atrazine in water through short-term exposure (7 days) on male black spotted frog (Pelophylax nigromaculatus) adults fed every day. The jumping ability, including jumping height, distance, time, and speed, was measured by 3D motion analysis software, and the intestinal content microbiota was determined by 16S rRNA amplicon sequencing with QIIME software. The results showed that male P. nigromaculatus exposure to 200 and 500 μg/L atrazine significantly increased jumping distance and jumping time compared to control groups. Conversely, 500 μg/L atrazine treatments significantly decreased the diversity and changed the composition and structure of intestinal content microflora in male P. nigromaculatus compared to control groups. At the phylum level, Chlamydiae was only detected in the control group, and Actinobacteria, Bacteroidetes, Firmicutes, Fusobacteria, and Proteobacteria were the dominant microflora in the atrazine treatment groups. At the genus level, the abundance of Lactobacillus and Weissella significantly increased in atrazine treatment groups compared to control groups. This study can provide a new framework based on movement behavior and intestinal microbiota to evaluate the response of amphibians to short-term exposure to environmental pollution.