The toxicity of linear alkylbenzene sulfonates (LABs) to Clarias gariepinus was investigated. For 30 days, the fish were exposed to LABs at 0.00, 0.50, 1.00, 1.50, and 2.00 mg/L. After each trial period, one fish from each plastic tub was chosen and its heart was punctured for blood samples. The blood samples were then collected and deposited in pre-designated bottles for analysis. Following blood collection, a fish was dissected and its organs were extracted. The organs were preserved in liquid nitrogen at -25oC until they were analyzed. A portable refractometer was used to quantify total serum protein content. A microplate reader was used to measure reduced glutathione (GSH). Albumin was quantified using the Bromocresol Green albumin assay kit, whereas alanine aminotransferase activity was assessed colorimetrically. Subtracting albumin from protein concentration yielded the globulin content. On days 23 and 30, protein content corresponds positively with exposure length and differs significantly (p < 0.05) between the control and treatment groups. The activity of GSH reduced slightly but not significantly (p > 0.05). Significant variations in albumin and globulin (p < 0.05) only on day 30. AAS activity differs significantly (p < 0.05) between treatments and the control. This study demonstrated that LAB exposure can be harmful to human health. Because anthropogenic sources are the primary source of LAB exposure, authorities must implement strict mitigation measures to limit this risk.

Information on the biological responses of polyploid animals towards environmental contaminants is scarce. This study aimed to compare reproductive axis-related gene expressions in the brain, plasma biochemical responses, and the liver and gill histopathological alterations in diploid and triploid full-sibling juvenile African catfish (Clarias gariepinus). Fish were exposed for 96 h to one of the two waterborne phenanthrene (Phe) concentrations [mean measured (SD): 6.2 (2.4) and 76 (4.2) μg/L]. In triploids, exposure to 76 μg/L Phe increased mRNA level of fushi tarazu-factor 1 (ftz-f1). Expression of tryptophan hydroxylase2 (tph2) was also elevated in both ploidies following the exposure to 76 μg/L Phe compared to the solvent control. In triploids, 76 μg/L Phe increased plasma alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) levels compared to the other Phe-exposed group. It also elevated lactate and glucose contents relative to the other groups. In diploids, however, biochemical biomarkers did not change. Phenanthrene exposures elevated glycogen contents and the prevalence of histopathological lesions in the liver and gills of both ploidies. This study showed substantial differences between diploids and triploids on biochemical and molecular biomarker responses, but similar histopathological alterations following acute Phe exposures.

Skin is a major by-product of the fisheries and aquaculture industries and is a valuable source of gelatin. This study examined the effect of triploidization on gelatin yield and proximate composition of the skin of African catfish (Clarias gariepinus). We further investigated the effects of two commonly used pesticides, chlorpyrifos (CPF) and butachlor (BUC), on the skin gelatin yield and amino acid composition in juvenile full-sibling diploid and triploid African catfish. In two separate experiments, diploid and triploid C. gariepinus were exposed for 21 days to graded CPF [mean measured: 10, 16, or 31 μg/L] or BUC concentrations [Mean measured: 22, 44, or 60 μg/L]. No differences in skin gelatin yield, amino acid or proximate compositions were observed between diploid and triploid control groups. None of the pesticide treatments affected the measured parameters in diploid fish. In triploids, however, gelatin yield was affected by CPF treatments while amino acid composition remained unchanged. Butachlor treatments did not alter any of the measured variables in triploid fish. To our knowledge, this study is the first to investigate changes in the skin gelatin yield and amino acid composition in any animal as a response to polyploidization and/or contaminant exposure.

Microplastic ingestion has been documented in various aquatic species. This causes physical damage, and additionally contaminated microplastics transfer attached pollutants and microbial pathogens to ingesting organisms. Continued metal accumulation can lead to toxicity and adverse health effects; attached microbial pathogens can cause dysbiosis - which lowers host immunity and promotes infections. Catfish, Clarias gariepinus, are a major food source in Southeast Asia, a hotspot of plastic pollution. This study aimed to quantify the transfer of the trace metals copper (Cu) and lead (Pb) -at environmentally relevant concentrations-from microplastics (polyamide 12, PA12, and polylactic acid, PLA) to catfish. Fish were reared for three months and exposed to seven different combinations of feed, supplemented with plastics and metals. At monthly intervals, fish gills, intestines, liver, and edible muscles were analysed for Cu and Pb concentrations using ICP-OES, and the intestines content assessed for Vibrio sp.. Our results showed that biodegradable PLA transferred higher amounts of metals to catfish than expected and also led to increased Vibrio counts in the intestines compared to PA12. Trace metal accumulation was significantly different in varying tissues, with highest concentrations observed in the gills, followed by liver, intestines, and lastly edible muscles. The results of this study further support the existing evidence that microplastics act as efficient shuttles to concentrate and transfer metals. They also indicate that their uptake can cause dysbiosis (increased numbers of Vibrio sp.). Most importantly, however, our study highlights that biodegradable polymers, such as PLA, could actually pose a greater environmental threat when ingested compared to the more common polymers such as PA12.

Freshwater environments are more sensitive to anthropogenic influences and usually contain higher concentrations of pollutants than marine environments. Microplastic pollution causes additional stress on freshwater animals; yet, studies evaluating the microplastic occurrence in freshwater biota are still limited. In this study, microplastic occurrence in the gastrointestinal tracts (GIT) and gill of commercial fish species (Prussian carp Carassius gibelio (Bloch, 1782); Abu mullet Planiliza abu (Heckel, 1843); Common carp Cyprinus carpio Linnaeus, 1758; European ell Anguilla Anguilla (Linnaeus, 1758); North African catfish Clarias gariepinus (Burchell, 1822); Goldfish Carassius auratus (Linnaeus, 1758) were reported from Orontes River. MPs abundance in the GIT and gill of six species were found as 5.1 ± 2 MPs fish⁻¹ and 4.4 ± 2 MPs fish⁻¹ with an occurrence of 95% and 74%, respectively. The majority of extracted microplastics were fiber, black and less than 1000 μm in size. FTIR analysis determined the main polymer types as polyester (50%), high-density polyethylene (HDPE) (10%), polypropylene (PP) (8%) and polyethylene terephthalate (PET) (5%). High MPs abundance and frequency of occurence indicate the exposure of microplastic pollution in freshwater biota which could threat the health of both individuals and consumers. Results obtained in this study will increase the acknowledgement of MPs pollution in the Orontes River. Also, this study will provide data to the administrators to set up necessary legislations in freshwater ecosystems.

Heavy metals have been found in increasing concentrations in the aquatic environment. Fishes exposed to such metals have altered gene expression, serum profiles, tissue histology and bioindices that serve as overall health biomarkers. The heavy metals (Ni, Cd, and Cr) accumulated in water and fish tissues, were beyond the permissible limits defined by the Central Pollution Control Board/World Health Organization. Metallothionein (MT) and glutathione peroxidase (GPX) genes expression patterns highlighted the metal-specific exposure of fish. An increased fold change of genes against beta-actin serves as a potential feature for toxicity. Metal toxicity is also reflected by an increased level of digestive enzymes (amylase and lipase) in the serum and alterations in values of reproductive hormones (11-Ketotestosterone and progesterone). Total serum bilirubin attribute to the liver and biliary tract disease in fishes. Histopathological studies show cellular degeneration, breakage, vacuolization signifying the chronic stress.

Pyrazole derivatives display diverse biological and pharmacological activities. The aim of this study is to investigate the antioxidant properties of a novel pyrazolecarboxamide derivative (4-amino-N-[(4-chlorophenyl)]-3-methyl-1-phenyl-1H-thieno [2, 3-c] pyrazole-5-carboxamide) in African catfish, Clarias gariepinus, exposed to 1 mg/L PbNO₃. Fish were intramuscularly injected with pyrazole-5-carboxamidederivative according to the following groupings: Group 1 (control), Group 2 (1 mg/L lead nitrate), Group 3 (1 mg/L lead nitrate + 5 mg pyrazole derivative/kg body weight), and Group 4 (1 mg/L lead nitrate + 10 mg pyrazole derivative/kg body weight) for two weeks and four weeks. Lead nitrate (1 mg/L) caused significant elevation of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine, uric acid, cholesterol, and glucose-6-phosphate dehydrogenase (G6PDH) compared to the control group after two and four weeks of exposure, while serum total lipids, alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) were significantly reduced compared to the control group. Furthermore, levels of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) and total antioxidant capacity (TAC) were reduced in group 2 compared to the control group. However, in group 2, hepatic lipid peroxidation (LPO) and DNA fragmentation percentage were significantly increased compared to the control group. Histopathological changes in the liver of lead-exposed groups included marked disturbance of hepatic tissue organization, degeneration of hepatocytes, dilation of blood sinusoids and the central vein as well as necrosis. Injection of pyrazole derivative for two weeks and four weeks reversed alterations in biochemical parameters, antioxidant biomarkers, lipid peroxidation, hepatic DNA damage, and histopathological changes in liver tissue induced by 1 mg/L lead nitrate. This amelioration was higher in response to high-dose pyrazole derivative (10 mg) at the fourth week of exposure, showing concentration-and time-dependency. Overall, the sensitized derivative pyrazolecarboxamide is likely a useful tool to minimize the effects of lead toxicity due to its potent antioxidant activity.

This paper reports on the seasonal and species comparison of hepatic nodular alterations in two indicator fish species from the hyper-eutrophic Roodeplaat Dam in South Africa. This freshwater system is characterized by seasonal cyanobacterial algal blooms which release bio-toxins, including hepato-toxins, which can have negative effects on the health of the resident fish population. A total of 115 Clarias gariepinus (Burchell) and 98 Oreochromis mossambicus (Peters) were collected seasonally across six different surveys over a period of 3 years. Nodular alterations in livers were assessed both macroscopically and microscopically. We found a species difference with a 48% prevalence of nodular alterations in C. gariepinus and no macroscopically visible alterations in O. mossambicus. Affected fish also showed an increase in the ratio of liver mass to body mass, i.e., the hepatosomatic index. The microscopic characteristics of the nodules were primarily associated with pre-neoplastic, focal areas of cellular alterations; most prevalent were focal areas of steatosis. However, we could not establish a seasonal pattern regarding the occurrence of these alterations and therefore no association between the occurrence of the liver pathology and the cyanobacterial blooms. Our results therefore suggest that the occurrence of nodular alterations is not an acute, seasonal response, but rather a chronic, and possibly, and more interestingly, a species-specific, pathological response.

Act Force Gold®, Butaforce®, and Atraforce® are among the most commonly used pesticides in Nigeria. The lethal concentrations and the respective toxic units for the three pesticides were determined. The genotoxic effects of the three pesticides were investigated in the red blood cells of Clarias gariepinus using micronucleus (MN) assay. The 96 h LC₅₀ was 4.75, 4.84, and 54.74 mg L⁻¹ for Act Force Gold®, Butaforce®, and Atraforce®, respectively. The toxic units in ascending order of toxicity were 1.83, 20.66, and 21.05 for Act Force Gold®, Butaforce®, and Atraforce® respectively. The estimated safe levels based on NAS/NAE varied from 4.75 × 10⁻¹–4.75 × 10⁻⁵ in Act Force Gold® through 4.64 × 10⁻¹–4.85 × 10⁻⁵ in Butaforce® to 5.74–5.74 × 10⁻⁵ in Atraforce®. Fish specimens were exposed to the pesticides and sampling was done at regular intervals at days 1, 7, 14, and 21 and after another 7-day recovery period. The results obtained indicated concentration- and duration-dependent increase in % MN formation with maximum values of 3.40 ± 0.25 for Act Force Gold® on day 14 and 3.05 ± 0.36 and 2.35 ± 0.14 for Butaforce® and Atraforce® respectively on day 7 of exposure. The 7-day recovery period could not reverse the trend as the % MN values obtained were significantly different from the control. The results further support the use of MN assay in assessing the toxicity of aquatic pollutants and can be used in the monitoring of aquatic ecosystems.

This study is the first in forming an integrated up-to-date spatial analysis linking heavy metal contamination among three different compartments of Lake Edku ecosystem to human health effects. The study determined the concentrations of iron (Fe), nickel (Ni), lead (Pb), chromium (Cr), and cadmium (Cd) in sediment and water samples collected from 12 stations covering the entire lake, as well as in the liver and muscle tissues of the three commercially edible fish species (African sharptooth catfish (Clarias gariepinus), Blue tilapia (Oreochromis aureus), and Redbelly tilapia (Tilapia zillii)), during winter 2019. Cartographic maps investigating the spatial contamination pattern of each metal in sediment and water samples along the entire lake, as well as correlation analysis between metal pairs, were generated. Moreover, the study examined the affinity of metal transfer among the three compartments and assessed the probability of being exposed to non-carcinogenic health hazards from the consumption of the abovementioned species. Results revealed that Fe and Cd recorded the highest and lowest mean concentrations, respectively, in sediment, water, and fish samples. The mean concentrations of Cd, Pb, and Ni in the water of Lake Edku, as well as the mean concentrations of Cd, Pb, and Cr in muscle tissues of the three examined fish species, exceeded the permissible limits. The northwestern part of the lake exhibited the highest concentrations of Fe, Ni, and Cr in Lake Edku’s sediment with strong significant positive correlations recorded between Fe–Ni, Fe–Cr, and Ni–Cr pairs suggesting sharing the same contamination source. Concerning Lake Edku’s water, the northwestern area displayed the highest concentrations for all metals. Moreover, Cd, Pb, and Cr exhibited nearly the same spatial contamination pattern with the hotspot located in the western part of the lake. Strong significant positive correlations were found between Cd–Pb, Cd–Cr, and Pb–Cr pairs in surface water, suggesting sharing the same origin of dispersion. The three fish species accumulated heavy metals from water several folds higher than from sediments, indicating that water is the main source of the intensive transfer of heavy metals to the three fish species. Based on the consumption of the three investigated fish species, children recorded the highest estimated daily (EDI) and weekly intakes (EWI) of heavy metals followed by adults. Regardless Pb, EWI values indicated that there are no significant health risks through fish consumption from the examined metals recommending that children should consume less than 85.227 g/day African sharptooth catfish muscle, 108.696 g/day Blue tilapia muscle, and 97.403 g/day Redbelly tilapia muscle to assure their health. Moreover, adults should consume less than 397.727 g/day African sharptooth catfish muscle, 507.24 g/day Blue tilapia muscle, and 454.54 g/day Redbelly tilapia muscle. The estimated hazard quotient (HQ) of all metals was lower than or close to the safe values of one for children and adults in the three investigated fish species indicating the absence of potential non-carcinogenic threats. The hazard index (HI) estimated for the five examined heavy metals in each of the investigated fish species was below one in adults only. Regarding the total hazard index estimated from consuming the three fish species, both children and adults may be subjected to non-carcinogenic risk.