Уточнить поиск
Результаты 1-10 из 192
Rhizophagus irregularis enhances tolerance to cadmium stress by altering host plant hemp (Cannabis sativa L.) photosynthetic properties
2022
Sun, Simiao | Feng, Yuhan | Huang, Guodong | Zhao, Xu | Song, Fuqiang
Arbuscular mycorrhizal fungi (AMF) are widespread and specialized soil symbiotic fungi, and the establishment of their symbiotic system is of great importance for adversity adaptation. To reveal the growth and photosynthetic characteristics of AMF–crop symbionts in response to heavy metal stress, this experiment investigated the effects of Rhizophagus irregularis (Ri) inoculation on the growth, photosynthetic gas exchange parameters, and chlorophyll fluorescence characteristics of hemp (Cannabis sativa L.) at a Cd concentration of 80 mg/kg. The results showed that (1) under Cd stress, the biomass of each plant structure in the Ri treatment was significantly higher than that in the noninoculation treatment (P < 0.05); (2) under Cd stress, the transpiration rate, stomatal conductance, net photosynthetic rate, PSII efficiency, apparent electron transport rate and photochemical quenching coefficient of the Ri inoculation group reached a maximum, with increases ranging from 1% to 28%; (3) inoculation of Ri significantly reduced Cd enrichment in leaves, which in turn significantly increased the transpiration rate, stomatal conductance, electron transfer rate, net photosynthetic rate and photosynthetic intensity, protecting PSII (P < 0.05); and (4) by measuring the light response curves of different treatments, the light saturation points of hemp inoculated with the Ri treatment reached 1448.4 μmol/m²/s, and the optical compensation point reached 24.0 μmol/m²/s under Cd stress. The Ri–hemp symbiont demonstrated high adaptability to weak light and high utilization efficiency of strong light under Cd stress. Our study showed that Ri–hemp symbiosis improves adaptation to Cd stress and promotes plant growth by regulating the photosynthetic gas exchange parameters and chlorophyll fluorescence parameters of plants. The Ri–hemp symbiosis is a promising technology for improving the productivity of Cd-contaminated soil.
Показать больше [+] Меньше [-]Insights into phenol monomers in response to electron transfer capacity of humic acid during corn straw composting process
2022
Zhao, Xinyu | Zhang, Chuanyan | Dang, Qiuling | Xi, Beidou
Quinone is the important redox functional group for electron transfer capacity (ETC) of humic acid (HA). Lignin, as major component in corn straw, can be decomposed into phenol monomers, then oxidation into quinones for synthesis of HA during composting process. However, it is still unclear that the effects of type and variation characteristics of phenol monomers on redox characteristics of HA during straw composting process. In this study, p-hydroxybenzoic acid (P1), vanillic acid (P2), syringic acid (P3), p-hydroxy benzaldehyde (P4), 4-coumaric acid (P5), 4-hydroxyacetophenone (P6), ferulic acid (P7) and 4-hydroxy-3-methylacetophenone (P8) were recognized and clustered into three groups. The concentration of polyphenol presented a significant downward trend during the straw composting process. Based on the relationships among phenol monomers to ETC, electron donating capacity (EDC), electron accepting capacity (EAC) and quinone, we found that P1, P2, P3, P5 and P7 were significantly related to ETC, EDC and EAC of HA (P < 0.05). Furthermore, NH₄⁺-N and NO₃⁻-N were the main micro-environmental factors linking to ETC-related phenol monomers and redox characteristics of HA in straw composts (P < 0.05). Finally, two groups of core microflora that promoting the ETC-related phenol monomers and NH₄⁺-N, and ETC-related phenol monomers and NO₃⁻-N were identified by Mantel test, respectively. This study contributes a new insight for polyphenol way for redox capacity of HA in traditional composting and utilization of straw compost in contaminated environments.
Показать больше [+] Меньше [-]Comparing with oxygen, nitrate simplifies microbial community assembly and improves function as an electron acceptor in wastewater treatment
2022
Zheng, Lei | Wang, Xue | Ren, Mengli | Yuan, Dongdan | Tan, Qiuyang | Xing, Yuzi | Xia, Xuefeng | Xie, En | Ding, Aizhong
Biochemical oxidation and reduction are key processes in treating biological wastewater and they require the presence of electron acceptors. The functional impact of electron acceptors on microbiomes provides strategies for improving the treatment efficiency. This research focused on two of the most important electron acceptors, nitrate and oxygen. Molecule ecological network, null model, and functional prediction based on high-throughput sequencing were used to analyze the microbiomes features and assembly mechanism. The results revealed nitrate via the homogeneous selection (74.0%) decreased species diversity, while oxygen via the homogeneous selection (51.1%) and dispersal limitation (29.6%) increased the complexity of community structure. Microbes that were more strongly homogeneously selected for assembly included polyphosphate accumulating organisms (PAOs), such as Pseudomonas and variovorax in the nitrate impacted community; Pseudomonas, Candidatus_Accumulibacter, Thermomonas and Dechloromonas, in the oxygen impacted community. Nitrate simplified species interaction and increased the abundance of functional genes involving in tricarboxylic acid cycle (TCA cycle), electron transfer, nitrogen metabolism, and membrane transport. These findings contribute to our knowledge of assembly process and interactions among microorganisms and lay a theoretical basis for future microbial regulation strategies in wastewater treatment.
Показать больше [+] Меньше [-]Polyethylenimine-grafted nitrogen-doping magnetic biochar for efficient Cr(VI) decontamination: Insights into synthesis and adsorption mechanisms
2022
Qu, Jianhua | Zhang, Xiubo | Bi, Fuxuan | Wang, Siqi | Zhang, Xinmiao | Tao, Yue | Wang, Yifan | Zhao, Jiang | Zhang, Ying
Herein, polyethylenimine (PEI)-grafted nitrogen (N)-doping magnetic biochar (PEIMW@MNBCBM) was synthesized, and characterization results showed that the microwave-assisted PEI grafting and ball milling-assisted N doping introduced abundant amino, pyridine N and pyrrole N structures onto biochar, which possessed high affinity to Cr(VI) in the anion form. The as-prepared PEIMW@MNBCBM displayed pH-dependence adsorption performance and high tolerance to co-existing ions with maximum uptake capacity of Cr(VI) identified as 183.02 mg/g. Furthermore, PEIMW@MNBCBM could bind Cr(VI) through electrostatic attraction, complexion, precipitation, reduction and pore filling. Especially, effective reduction of Cr(VI) was ascribed to cooperative electron transfer of partial oxygen-containing functional groups, intramolecular pyridine/pyrrole N, protonated amino and Fe²⁺ on the adsorbent, while oxygen-containing and amino functional groups from N-doping biochar and PEI synergistically complexed Cr(III) via providing lone pair electrons to form coordinate bonds. Furthermore, the stable precipitation was formed between Fe³⁺ and Cr(III). Additionally, the Cr(VI) elimination efficiency could maintain 95.83% even after four adsorption-desorption cycles, suggesting PEIMW@MNBCBM as a high-performance adsorbent for Cr(VI) contaminated water remediation.
Показать больше [+] Меньше [-]Bioelectrochemical system for dehalogenation: A review
2022
Zhu, Xuemei | Wang, Xin | Li, Nan | Wang, Qi | Liao, Chengmei
Halogenated organic compounds are persistent pollutants, whose persistent contamination and rapid spread seriously threaten human health and the safety of ecosystems. It is difficult to remove them completely by traditional physicochemical techniques. In-situ remediation utilizing bioelectrochemical technology represents a promising strategy for degradation of halogenated organic compounds, which can be achieved through potential modulation. In this review, we summarize the reactor configuration of microbial electrochemical dehalogenation systems and relevant organohalide-respiring bacteria. We also highlight the mechanisms of electrode potential regulation of microbial dehalogenation and the role of extracellular electron transfer in dehalogenation process, and further discuss the application of bioelectrochemical technology in bioremediation of halogenated organic compounds. Therefore, this review summarizes the status of research on microbial electrochemical dehalogenation systems from macroscopic to microscopic levels, providing theoretical support for the development of rapid and efficient in situ bioremediation technologies for halogenated organic compounds contaminated sites, as well as insights for the removal of refractory fluorides.
Показать больше [+] Меньше [-]A new understanding of the microstructure of soot particles: The reduced graphene oxide-like skeleton and its visible-light driven formation of reactive oxygen species
2021
Zhu, Jiali | Shang, Jing | Zhu, Tong
The mechanisms of soot’s photochemistry are still unclear, especially, how the microstructure and composition of soot influence its photoactivity. In the current study, we started with the exploration of the microstructure of soot particles and gained new insights. The elemental-carbon fraction of soot (E-soot), considered the core component of soot and can reflect the intrinsic characteristics of soot, was extracted by organic solvents and characterized in terms of structure and chemical reactivity. The intrinsic structure of E-soot was found to be more analogous to reduced graphene oxide than to graphene, in terms of containing similar levels of defective sites such as oxygen-containing functional groups and environmentally persistent free radicals, as well as exhibiting similar optoelectronic performance. The generation of reactive oxygen species via an electron transfer pathway under visible light suggests that reduced graphene oxide-like E-soot can serve as a potential carbo-photocatalyst, which facilitates elucidating the mechanism of E-soot’s role during soot’s photochemical aging. Our study reveals the intrinsic structure of soot and its role in photo-triggered reactive oxygen species production, which is vital for atmospheric and health effects.
Показать больше [+] Меньше [-]1H-NMR metabolomics profiling of zebra mussel (Dreissena polymorpha): A field-scale monitoring tool in ecotoxicological studies
2021
Hani, Younes Mohamed Ismail | Prud’Homme, Sophie Martine | Nuzillard, Jean-Marc | Bonnard, Isabelle | Robert, Christelle | Nott, Katherine | Ronkart, Sébastien | Dedourge-Geffard, Odile | Geffard, Alain
Biomonitoring of aquatic environments requires new tools to characterize the effects of pollutants on living organisms. Zebra mussels (Dreissena polymorpha) from the same site in north-eastern France were caged for two months, upstream and downstream of three wastewater treatment plants (WWTPs) in the international watershed of the Meuse (Charleville-Mézières “CM” in France, Namur “Nam” and Charleroi “Cr” in Belgium). The aim was to test ¹H-NMR metabolomics for the assessment of water bodies’ quality. The metabolomic approach was combined with a more “classical” one, i.e., the measurement of a range of energy biomarkers: lactate dehydrogenase (LDH), lipase, acid phosphatase (ACP) and amylase activities, condition index (CI), total reserves, electron transport system (ETS) activity and cellular energy allocation (CEA). Five of the eight energy biomarkers were significantly impacted (LDH, ACP, lipase, total reserves and ETS), without a clear pattern between sites (Up and Down) and stations (CM, Nam and Cr). The metabolomic approach revealed variations among the three stations, and also between the upstream and downstream of Nam and CM WWTPs. A total of 28 known metabolites was detected, among which four (lactate, glycine, maltose and glutamate) explained the observed metabolome variations between sites and stations, in accordance with chemical exposure levels. Metabolome changes suggest that zebra mussel exposure to field contamination could alter their osmoregulation and anaerobic metabolism capacities. This study reveals that lactate is a potential biomarker of interest, and ¹H-NMR metabolomics can be an efficient approach to assess the health status of zebra mussels in the biomonitoring of aquatic environments.
Показать больше [+] Меньше [-]Stomatal response drives between-species difference in predicted leaf water-use efficiency under elevated ozone
2021
Xu, Yansen | Shang, Bo | Peng, Jinlong | Feng, Zhaozhong | Tarvainen, Lasse
Ozone-induced changes in the relationship between photosynthesis (Aₙ) and stomatal conductance (gₛ) vary among species, leading to inconsistent water use efficiency (WUE) responses to elevated ozone (O₃). Thus, few vegetation models can accurately simulate the effects of O₃ on WUE. Here, we conducted an experiment exposing two differently O₃-sensitive species (Cotinus coggygria and Magnolia denudata) to five O₃ concentrations and investigated the impact of O₃ exposure on predicted WUE using a coupled Aₙ-gₛ model. We found that increases in stomatal O₃ uptake caused linear reductions in the maximum rates of Rubisco carboxylation (Vcₘₐₓ) and electron transport (Jₘₐₓ) in both species. In addition, a negative linear correlation between O₃-induced changes in the minimal gₛ of the stomatal model (g₀) derived from the theory of optimal stomatal behavior and light-saturated photosynthesis was found in the O₃-sensitive M. denudata. When the O₃ dose-based responses of Vcₘₐₓ and Jₘₐₓ were included in a coupled Aₙ-gₛ model, simulated Aₙ under elevated O₃ were in good agreement with observations in both species. For M. denudata, incorporating the O₃ response of g₀ into the coupled model further improved the accuracy of the simulated gₛ and WUE. In conclusion, the modified Vcₘₐₓ, Jₘₐₓ and g₀ method presented here provides a foundation for improving the prediction for O₃-induced changes in Aₙ, gₛ and WUE.
Показать больше [+] Меньше [-]Carbon cloth facilitates semi-continuous anaerobic digestion of organic wastewater rich in volatile fatty acids from dark fermentation
2021
Feng, Dong | Xia, Ao | Liao, Qiang | Nizami, Abdul-Sattar | Sun, Chihe | Huang, Yun | Zhu, Xianqing | Zhu, Xun
The anaerobic digestion of wastewater rich in volatile fatty acids (VFAs) provides a sustainable approach for methane production whilst reducing environmental pollution. However, the anaerobic digestion of VFAs may not be stable during long-term operation under a short hydraulic retention time. In this study, conductive carbon cloth was supplemented to investigate the impacts on the anaerobic digestion of VFAs in wastewater sourced from dark fermentation. The results demonstrated that the failure of anaerobic digestion could be avoided when carbon cloth was supplemented. In the stable stage, the methane production rate with carbon cloth supplementation was improved by 200–260%, and the chemical oxygen demand (COD) removal efficiency was significantly enhanced compared with that in the control without carbon cloth. The relative abundance of potential exoelectrogens on the carbon cloth was increased by up to 8-fold compared with that in the suspension. Electrotrophic methanogens on the carbon cloth were enriched by 4.2–17.2% compared with those in the suspension. The genera Ercella and Petrimonas along with the methanogenic archaea Methanosaeta and Methanosarcina on the carbon cloth may facilitate direct interspecies electron transfer, thereby enhancing methane production.
Показать больше [+] Меньше [-]Biological iron nitrogen cycle in ecological floating bed: Nitrogen removal improvement and nitrous oxide emission reduction
2021
Sun, Shanshan | Gu, Xushun | Zhang, Manping | Tang, Li | He, Shengbing | Huang, Jungchen
Ecological floating beds (EFBs) have become a superior method for treating secondary effluent from wastewater treatment plant. However, insufficient electron donor limited its denitrification efficiency. Iron scraps from lathe cutting waste consist of more than 95% iron could be used as electron donors to enhance denitrification. In this study, EFBs with and without iron scraps supplementation (EFB-Fe and EFB, respectively) were conducted to explore the impacts of iron scraps addition on nitrogen removal, nitrous oxide (N₂O) emissions and microbial communities. Results showed the total nitrogen (TN) removal in EFB-Fe improved to 79% while that in EFB was 56%. N₂O emission was 0–6.20 mg m⁻² d⁻¹ (EFB-Fe) and 1.74–15.2 mg m⁻² d⁻¹ (EFB). Iron scraps could not only improve nitrogen removal efficiency, but also reduce N₂O emissions. In addition, high-throughput sequencing analysis revealed that adding iron scraps could improve the sum of denitrification related genera, among which Novosphingobium accounted for the highest proportion (6.75% of PFe1, 4.24% of PFe2, 3.18% of PFe3). Iron-oxidizing bacteria and iron-respiring bacteria associated with and nitrate reducing bacteria mainly concentrated on the surface of iron scraps. Principal co-ordinates analysis (PCoA) indicated that iron scraps were the key factor affecting microbial community composition. The mechanism of iron scraps enhanced nitrogen removal was realized by enhanced biological denitrification process. Iron release dynamic from iron scraps was detected in bench-scale experiment and the electron transfer mechanism was that Fe⁰ transferred electrons directly to NO₃⁻-N, and biological iron nitrogen cycle occurred in EFB-Fe without secondary pollution.
Показать больше [+] Меньше [-]