International audience | Apple orchards are highly manipulated crops in which large amounts of pesticides are used. Some of these pesticides lack target specificity and can cause adverse effects in non-target organisms. In order to evaluate the environmental risk of these products, the use of transplanted sentinel organisms avoids side-effects from past events and facilitate comparison of multiple sites in a short time. We released specimens of the terrestrial snail <em>Xeropicta derbentina</em> in each 5 of two kinds of apple orchards with either conventional or organic management strategies plus in a single abandoned orchard. After one month, individuals were retrieved in order to measure acetylcholinesterase (AChE) activity. Mean values of AChE activity were significantly reduced in all conventional apple orchards compared to the others. Results show that the measurement of biomarkers such as AChE inhibition in transplated <em>X. derbentina</em> could be useful in the environmental risk assessment of post-authorized pesticides

Bio-based plastics have been developed as alternative materials to solve the energy crisis brought by plastic production, but their impacts on soil ecosystems (e.g. plant and microorganisms) remain largely unknown. Here, we conducted study on the impacts of polyethylene 2,5-furan-dicarboxylate (PEF), a new bio-based plastic, on the plant-soil ecosystem, with comparison of fossil-based plastic polyethylene terephthalate (PET). Our investigation showed that, after 21 days exposure to microplastics (MPs) at doses of 0.5%, 1% and 2%, both PEF and PET MPs inhibited the growth of lettuce, where chlorophyll was found to be the most sensitive index. According to the comprehensive stress resistance indicators, PET MPs showed more severe phytotoxicity than PEF MPs. Although both PEF and PET MPs could inhibit soil enzyme activities, PET MPs exhibited significantly reduction on the diversity of rhizosphere soil bacterial community and changed the relative abundance of dominant species. Our study gave insights into the effects of PEF and PET MPs on the plant-soil system, where bio-based PEF MPs showed more friendly interaction with plant and soil than fossil-based PET MPs. Our results provided scientific data for risk assessment and useful information for the prospective application of bio-based plastics.

A pot experiment was carried out on brown soil polluted by dibutyl phthalate (DBP) and di-(2-ethylhexyl) phthalate (DEHP) to investigate the effects of biochar (BC) derived from corn straw and Fe–Mn oxide modified biochar composites (FMBC) on the bioavailability of DBP and DEHP, as well as ecosystem responses in rhizosphere soil after wheat ripening. The results indicate that the application of BC and FMBC significantly increases soil organic matter, pH, available nitrogen (AN), Olsen phosphorus, and available potassium (AK); reduces the bioavailability of DBP and DEHP; enhances the activities of dehydrogenase, urease, protease, β-glucosidase, and polyphenol oxidase; and decreases acid phosphatase activity. No changes in richness and diversity, which were measured by Illumina MiSeq sequencing, were observed following BC and FMBC application. The bacterial community structure and composition varied with DBP/DEHP concentrations and BC/FMBC additions in a nonsystematic way and no significant trends were observed. In addition, FMBC exhibited better performance in increasing soil properties and decreasing the bioavailability of DBP and DEHP compared with BC. Hence, the FMBC amendment may be a promising way of developing sustainable agricultural environmental management.

Pesticides scarcely exist as individual compounds in the water ecosystem, but rather as mixtures of multiple chemicals at relatively low concentrations. In this study, we aimed to explore the mixture toxic effects of fludioxonil (FLU) and triadimefon (TRI) on zebrafish (Danio rerio) by employing different toxicological endpoints. Results revealed that the 96-h LC₅₀ values of FLU to D. rerio at multiple developmental stages ranged from 0.055 (0.039–0.086) to 0.61 (0.33–0.83) mg L⁻¹, which were less than those of TRI ranging from 3.08 (1.84–5.96) to 9.75 (5.99–14.78) mg L⁻¹. Mixtures of FLU and TRI exerted synergistic effects on embryonic zebrafish. Activities of total superoxide dismutase (T-SOD) and catalase (CAT) were markedly altered in most of the individual and pesticide mixture treatments compared with the control. The expressions of 16 genes involved in oxidative stress, cellular apoptosis, immune system and endocrine system displayed that embryonic zebrafish were affected by the individual pesticides and their mixtures, and greater variations of four genes (ERɑ, Tnf, IL and bax) were found when exposed to pesticide mixtures compared with their individual compounds. Therefore, more studies on mixture toxicities among different pesticides should be taken as a priority when evaluating their ecological risk.

The performance, microbial community and enzymatic activity of sequencing batch reactors (SBRs) were investigated under 75-day exposure of different Cu(II) concentrations. Cu(II) at 0–5 mg/L had no distinct impact on the chemical oxygen demand (COD) and nitrogen removal, oxygen-uptake rate (OUR), nitrification and denitrification rate, and microbial enzymatic activity. The inhibitory effects of Cu(II) at 10 and 30 mg/L on the nitrogen removal rate, OUR, and microbial enzymatic activity of SBR increased with an increment in operation time due to the Cu(II) biotoxicity and the accumulation of Cu(II) in activated sludge. The changes of microbial reactive oxygen species production, lactate dehydrogenase release, catalase activity and superoxide dismutase activity demonstrated that Cu(II) at 10 and 30 mg/L broke the equilibrium between the oxidation and antioxidation processes in microbial cells and also damaged the cytomembrance integrity, which could affect the COD and nitrogen removal performance and change normal microbial cell morphology. The Cu(II) in the influent could be removed by the microbial absorption and accumulated in the activated sludge under long-term exposure. The microbial community displayed some distinct changes from 0 to 30 mg/L Cu(II). In contrast with 0 mg/L Cu(II), Nitrosomonas, Nitrospira and some denitrifying bacteria obviously decreased in relative abundance under long-term exposure of 10 and 30 mg/L Cu(II).

Perfluorooctanoic acid (PFOA) is an emerging persistent organic pollutant which has been identified at significant levels in soils. Existed ecotoxicological studies have mainly employed earthworms to evaluate the toxicity of PFOA. However, little information do we know about the toxicity of PFOA regarding soil microorganisms. Accordingly, the adverse effects of PFOA on microbial activity in a wheat soil under four fertilization treatments were investigated in this study. The microcalorimetric results revealed that the toxicity of PFOA on soil microbial activity in four treatments followed a descending sequence: Control (no fertilization), NK (no P fertilizer, but N and K fertilizers were used), PK (no N fertilizer, but P and K fertilizers were used), and NPK (N, P and K fertilizers were used). The soil sample with higher available P content had higher resistant to PFOA. There were significant differences in urease activity and alkaline phosphatase activity among the four fertilization treated soils. Molecular modeling studies clearly demonstrated that the binding of PFOA with alkaline phosphatase was more stable than with urease through electrostatic interaction, van der Waals force, and hydrogen bonds. These results are expected to provide more comprehensive information in toxicity of PFOA in soil environment.