River floodplain ecosystems host one of the highest freshwater molluscan biodiversity on Earth. However, multiple human disturbances, such as loss of hydrological connectivity and deterioration of water quality, are seriously threatening most floodplain lakes throughout the world. Given the high imperilment rate of freshwater molluscs but the scarcity of studies examining the anthropogenic effects on this fauna, we test the response of mollusc assemblages to river-lake disconnection and eutrophication in 30 lakes in the Yangtze River floodplain, China. The species richness of entire Mollusca, Gastropoda and Bivalvia and 6 dominant families were all much lower at disconnected lakes than that in connected lakes, and decreased with increasing water eutrophication. The assemblage structure differed significantly among four lake groups for datasets based on entire Mollusca, Gastropoda and Bivalvia, indicating the serious impacts of hydrological disconnection and eutrophication. Moreover, the connected lakes showed significantly lower values of average taxonomic distinctness (Δ⁺) but higher values of variation in taxonomic distinctness (Λ⁺) than disconnected lakes. Such variations were triggered by the extirpation of congeneric and endemic species (mainly from families Unionidae and Viviparidae), which giving a waring of the loss of mollusc endemism in this region. In general, the present study showed that river-lake disconnection and deterioration of water quality resulted in serious biodiversity declines of both gastropods and bivalves in the Yangtze River floodplain lakes. A systematic approach including restoration of river-lake connectivity and habitats and improvement of water quality should be implemented in the conservation planning in this large river floodplain.

Neonicotinoid insecticides can be transported from agricultural fields, where they are used as foliar sprays or seed treatments, to surface waters by surface or sub-surface runoff. Few studies have investigated the toxicity of neonicotinoid or the related butenolide insecticides to freshwater mollusk species. The current study examined the effect of neonicotinoid and butenolide exposures to the early-life stages of the ramshorn snail, Planorbella pilsbryi, and the wavy-rayed lampmussel, Lampsilis fasciola. Juvenile P. pilsbryi were exposed to imidacloprid, clothianidin, or thiamethoxam for 7 or 28 d and mortality, growth, and biomass production were measured. The viability of larval (glochidia) L. fasciola was monitored during a 48 h exposure to six neonicotinoids (imidacloprid, thiamethoxam, clothianidin, acetamiprid, thiacloprid, or dinotefuran), or a butenolide (flupyradifurone). The 7-d LC50s of P. pilsbryi for imidacloprid, clothianidin, and thiamethoxam were ≥4000 μg/L and the 28-d LC50s were ≥182 μg/L. Growth and biomass production were considerably more sensitive endpoints than mortality with EC50s ranging from 33.2 to 122.0 μg/L. The 48-h LC50s for the viability of glochidia were ≥456 μg/L for all seven insecticides tested. Our data indicate that neonicotinoid and butenolide insecticides pose less of a hazard with respect to mortality of the two species of mollusk compared to the potential hazard to other non-target aquatic insects.

Unregulated use of chlorpyrifos (CPF) and monocrotophos (MCP) in agriculture casts adverse effects on non-target freshwater mollusc, Pila globosa and humans. Levels of CPF and MCP were assessed in the paddy field from the edible foot tissue of apple snail (Pila globosa) exposed to low (1.5 ml l⁻¹ water) and high (2.5 ml l⁻¹ water) agricultural doses for 48 h to determine human health risk associated with consumption of tissue. CPF and MCP were extracted by liquid–liquid extraction and analysed by QuEChERS method using GC–MS/MS. For low and high concentrations of CPF exposure, the pesticide residue levels in the paddy field water ranged from 4.43 to 1.08 and 5.13 to 1.53 µg l⁻¹, respectively, whereas, for low and high concentrations of MCP exposure, the residue levels in water ranged from 16.43 to 5.78 and 31.41 to 9. 27 µg l⁻¹, respectively, for 3–48 h. In the foot tissue, residues ranged from 4.36 to 15.54 µg kg⁻¹ for low-dose CPF, 7.1 to 18.05 µg kg⁻¹for high-dose CPF and from 5.28 to 12.3 µg kg⁻¹ and 8.94 to 18.21 µg kg⁻¹ for low and high dose of MCP, respectively, during 3 to 48 h of exposure. Pesticides in the tissue were lower than the recommended maximum residue limits. Estimated health risk for adults and children revealed that the estimated daily intake values did not exceed the threshold values of acceptable daily intake. Non-carcinogenic and carcinogenic health effects were less than the safe value of 1.0 and 1 × 10⁻⁶, respectively, suggesting that CPF and MCP residues from ingestion of apple snail posed low risks to both children and adults. This preliminary result suggests regular monitoring of pesticides residues in Pila globosa collected from the paddy field of India.

Tamoxifen is used to treat breast cancer and infertility. It acts as an estrogen antagonist or agonist, depending on the target species. Tamoxifen has been detected in aquatic environments, which causes sexual distortion in aquatic animals by affecting their hormone balance in the body. The influence of tamoxifen on golden apple snail Pomacea canaliculata Lamarck (Caenogastropoda: Ampullariidae), a freshwater mollusk, is still unclear. The purpose of this study was to determine whether tamoxifen had an estrogenic effect on P. canaliculata, and to evaluate the extent of tamoxifen’s influence on their reproductive and hormonal responses. The results showed that tamoxifen significantly decreased the contents of various hormones in the snails. The contents of estradiol, progesterone, testosterone, and 5-hydroxytryptamine were decreased to trace levels. The number of laying eggs by female snails was greatly reduced by tamoxifen, and the lowest fecundity was only 42.28. The hatching rate was reduced to 15.8% by tamoxifen treatment. Tamoxifen was ecotoxicity to the golden apple snail by disrupting the dynamic balance of key hormones, affecting social and mating behaviors of the snails. Therefore, it is important to prevent tamoxifen from being discharged into the aquatic ecosystem, to prevent residual tamoxifen from causing aquatic ecotoxicity.

The objectives of the survey were to analyse the structure of the mollusc communities in the mining subsidence reservoirs that were created as a result of land subsidence over exploited hard coal seams and to determine the most predictive environmental factors that influence the distribution of mollusc species. The reservoirs are located in urbanised and industrialised areas along the Trans-Regional Highway, which has a high volume of vehicular traffic. They all have the same sources of supply but differ in the physical and chemical parameters of the water. In total, 15 mollusc species were recorded including four bivalve species. Among them Anodonta cygnea is classified as Endangered according to the Polish Red Data Book of Animals and also as Near Threatened according to the European Red List of Non-marine Molluscs. Eleven of the 15 mollusc species are included on the European Red List of Non-marine Molluscs as Least Concern. Conductivity, pH and the concentration of calcium were the parameters most associated with the distribution of mollusc species. Canonical correspondence analysis showed that Potamopyrgus antipodarum, Radix balthica, Physella acuta, Gyraulus crista and Pisidium casertanum were associated with higher conductivity and lower pH values. A. cygnea, Anodonta anatina and Ferrissia fragilis were negatively influenced by these parameters of the water. The results of this survey showed that the mining subsidence reservoirs located in urbanised and industrialised areas provide refuges for rare and legally protected species and that they play an essential role in the dispersal of alien species as well.

The biocide triclosan (TCS, 5-chloro-2-(2,4-dichlorophenoxy)phenol) is commonly used in several personal care products, textiles, and children’s toys. Because the removal of TCS by wastewater treatment plants is incomplete, its environmental fate is to be discharged into freshwater ecosystems, where its ecological impact is largely unknown. The aim of this study was to determine the effect of TCS on the antioxidant enzymatic chain of the freshwater mollusk zebra mussel (Dreissena polymorpha). We measured the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), as well as the phase II detoxifying enzyme glutathione S-transferase (GST) in zebra mussel specimens exposed to 1 nM, 2 nM, and 3 nM TCS in vivo. The mussels were exposed for 96 h, and the enzyme activities were measured every 24 h. We measured clear activation of GST alone at all three dose levels, which shows a poor induction of the antioxidant enzymatic chain by TCS. CAT and SOD were activated only at 3 nM, while GPx values overlapped the baseline levels.

While biomarkers are undeniably key tools in aquatic ecotoxicology to measure adverse effects linked to contamination events, their application is often inhibited by monetary constraints negating the possibility of having access to dedicated equipment, special wares, and/or expensive reagents. To offset this bottleneck, we propose three simple physiological biomarkers, quantifiable in bivalves, that are free of cost considerations and that can provide basic knowledge on animal health and water quality. Indeed, condition index (CI), growth index (GI), and SOS response (air-time survival) comprise measurements straightforward enough to perform by any laboratory or science body on the planet. Long-term monitoring or screening studies can be carried out with these biomarkers and they are able to provide robust information notably after exposure of bivalves to either singular or multiple agents of contamination. By highlighting examples of data generated in aquatic studies conducted in Eastern Canada under both laboratory and field situations with different species of marine and freshwater mollusks, we establish the suitability of these biomarkers for assessing environmental contamination. Their relationships with other biomarkers are also shown which further corroborate their value as reliable indicators of ecosystem health.