The agroeconomic benefits of the routine use of triazole fungicides on crops have been evident for more than 40 years. However, increasing evidence shows that residues of triazoles are ubiquitous in various foods and thus could pose a potential health risk to humans. We analyzed 3406 samples of 13 food commodities that were collected from markets in 9 regions across China, and assessed the health risk of both chronic and acute exposure to the triazoles for Chinese children (1–6 years old) and the general population. Among all samples, 55.52% had triazoles in concentrations of 0.10–803.30 μg/kg, and 29.77% of samples contained a combination of 2–7 triazoles. Tebuconazole and difenoconazole were the most commonly found triazoles in the foods, being detected in 33.44% and 30.45% of samples, respectively. Chronic and acute cumulative risk assessment for total triazoles based on a relative potency factor method revealed that exposure to triazoles from these particular commodities was below the levels that might pose a health risk (chronic hazard index range, 5.90×10⁻⁷ to 1.83×10⁻³; acute hazard index range, 7.77×10⁻⁵ to 0.39, below 1). Notably, dietary exposure risk for children was greater than that for the general population—particularly for the acute intake of mandarin, grape, and cucumber (acute hazard index values of 0.35–0.39). Despite the low health risk, the potential hazards of exposure to triazoles should raise public concern owing to their ubiquitous presence in common foods and potential cumulative effects.

Evidence on the relationship between lifestyle, socio-economic factors and pesticide exposure and urinary concentrations of organophosphate (OP) pesticide metabolites among children is generally incomplete. This study investigated the relationship between socio-economic factors and reported pesticide exposures and the sum of three urinary concentrations of dialkyl phosphate metabolites (DAP) among boys living in the rural areas of the Western Cape, South Africa. Data was collected during a cross-sectional study of 183 boys from three agricultural intense areas. Measurements included a questionnaire on socio-economic and pesticide exposures and urinary DAP concentrations. Most boys (70%) lived on farms with a median age of 12 years (range: 5.0–19.5 years). Children aged >14 years had lower DAP urine concentrations (median = 39.9 ng/ml; β = −68.1 ng/ml; 95% CI: −136.8, 0.6) than children aged 9 years and younger (median = 107.0 ng/ml). DAP concentrations also varied significantly with area, with concentrations in the grape farming area, Hex River Valley (median = 61.8 ng/ml; β = −52.1; 95% CI: −97.9, −6.3 ng/ml) and the wheat farming area, Piketberg (median = 72.4 ng/ml; β = −54.2; 95% CI: 98.8, −9.7 ng/ml) lower than those in the pome farming area, Grabouw (median = 79.9 ng/ml). Other weaker and non-significant associations with increased DAP levels were found with increased household income, member of household working with pesticides, living on a farm, drinking water from an open water source and eating crops from the vineyard and or garden. The study found younger age and living in and around apple and grape farms to be associated with increased urinary DAP concentrations. Additionally, there were other pesticide exposures and socio-economic and lifestyle factors that were weakly associated with elevated urinary DAP levels requiring further study. The study provided more evidence on factors associated to urinary DAP concentrations especially in developing country settings.

Copper stress is one of the most severe heavy metal stresses in plants. Grapevine has a relatively higher copper tolerance than other fruit crops. However, there are no reports regarding the tolerance mechanisms of the ‘Shine Muscat’ (‘SM’) grape to a low degree of excess copper stress (Low-ECS). Based on the physiological indicators and multi-omics (transcriptome, proteome, metabolome, and microRNAome) data, 8 h (h) after copper treatment was the most severe stress time point. Nonetheless, copper stress was alleviated 64 h after treatment. Cu ion transportation, photosynthesis pathway, antioxidant system, hormone metabolism, and autophagy were the primary response systems in ‘SM’ grapevine under Low-ECS. Numerous genes and proteins, such as HMA5, ABC transporters, PMM, GME, DHAR, MDHAR, ARGs, and ARPs, played essential roles in the ‘SM’ grapevine's response to Low-ECS. This work was carried out to gain insights into the multi-omics responses of ‘SM’ grapevine to Low-ECS. This study provides genetic and agronomic information that will guide better vinery management and breeding copper-resistant grape cultivars.

In most European countries, each adult citizen drinks on average more than 20 L of wine every year. Three popular wine-growing areas (Aleksandrovac, Topola, and Orahovac) in Serbia were studied in order to investigate the abundance and uptake of elements from vineyard soil to plants. The specific activities of radionuclides (²²⁶Ra, ²³²Th, ⁴⁰K, ¹³⁷Cs, and ⁷Be) were measured in soil, leaves, and grape berries. ²²⁶Ra and ²³²Th were positively correlated with silt and clay and negatively correlated with sand content in soil. Specific activities of natural radionuclides were also negatively correlated with soil pH and CaCO₃. Significant correlations of ⁴⁰K and ¹³⁷Cs with organic matter in soil were found. Concentrations of fifteen metals (As, Cd, Co, Cr, Cu, Mn, Ni, Pb, Zn, Fe, K, Na, Ca, Mg, and Hg) were also measured in soil samples as well as in grapevine leaves. Analyzed soils were rich in Ni, Cu, Co, Cr, and Cd. High concentrations of Cu were probably caused by long-term use of Cu-based fungicides. Cu was correlated with Fe and organic matter content in soil. Soil-to-plant transfer factors (TF) were calculated to estimate the uptake of radionuclides and metals. Correlations obtained via PCA enable distinction between the sites Aleksandrovac and Topola relative to Orahovac. The first principal component (PC1) accounting for 30.70% of the total variance correlated significantly with soil pH (H₂O), contents of CaCO₃, Na, Ca, ⁴⁰K, and ²²⁶Ra in soil, as well as with ²²⁶Ra, Na, Ca in plants and TFCₐ. The second principal component (PC2), with total variance of 17.21%, was mainly correlated with variables pertaining to Mg, Co, and Cr in the soil and TFK.

Atrazine is one of the most used herbicides in China. It is a persistent organic pollutant but has been widely used on Chinese farmlands for a long time. To assess its dietary and ecological risks to human and environment, in this study, atrazine residues were extracted with acetonitrile and then plant samples were detected with gas chromatography coupled with mass spectrometry (GC-MS) and soil samples were determined with gas chromatography coupled with nitrogen-phosphorus detector (GC-NPD). The limit of quantification (LOQ) of the method was 0.01 mg/kg for all matrices. The recoveries ranged from 82.0 to 105.4% for plant samples and 75.6 to 85.6% for soil samples. The final residues of atrazine in all plant samples were lower than LOQ. Dietary risk assessment suggested that under good agricultural practices (GAP) conditions, intake of atrazine from apples, grapes, and tea would exhibit an acceptably low health risk on consumers. However, the final residues of atrazine in soil samples were <0.01–9.2 mg/kg, and the half-lives were 2.0–9.1 days. Based on the species sensitivity distribution (SSD) model, the potential affected fraction (PAF) of atrazine in soil samples ranges from 0.01 to 65.8%. Atrazine residues in 43.1% soil samples were higher than 0.11 mg/kg, which was the hazardous concentration for 5% of species (HC₅) of atrazine in soil. These results suggested that the ecological risks of atrazine in apples, grapes, and tea garden soil would exhibit a high risk on environmental species even under the same GAP conditions. This study could provide guidance for comprehensive risk assessment of atrazine properly used in apple, grape, and tea gardens.

Pesticides may cause a potential risk to human health when applied in excess to control pests, diseases, and weeds in crop fields. In the current study, conducted in the Aegean region of Turkey from 2012 to 2016, a total of 3044 samples of 16 different commodities of fruits and vegetables were screened to identify pesticide residues and health risk to consumers posed by such residues was assessed. Results showed that 354 samples out of the total samples had higher maximum residue limit (MRL) values, while the MRL values were lower in 473 samples. In the study, residues of 64 different pesticides detected in 3044 samples in which 11.6% samples exceeded maximum residue limit (MRL) levels as compared with that of the approved MRL level by the Turkish authorities. Out of total samples, number of samples having high MRL level were as follows: 74 for chlorpyrifos (2.43%), 145 for azoxystrobin (3.8%), 112 for triadimenol (3.8%), 103 for carbendazim (3.4%), 98 for chlorpyrifos (3.2%), 94 for pyrimethanil (3.10%), 90 for cyprodinil (2.9%), 76 for fludioxonil (2.50%), 75 for indoxacarb (2.40%), 66 for imidacloprid (2.10%), and 60 for boscalid (1.90%). Residues of one, two, three, four, and even more than five pesticides were detected, respectively, in 16.1, 5.8, 2.8, 1, and 1.7% of the total test samples. The lowest estimated daily intake (EDI) values ranged from 3.57×10⁻³ to 8.98. The lower values of hazard quotient (HQ) were obtained in dried apricot, grape, and strawberry with the value of 0.01, although the HQ value in 32 out of 62 pesticides tested was found to be close to 0.

This study was performed in organic vineyard to assess integrated pollution in soil–plant–air system by potentially toxic elements (PTE). Concentrations of 26 PTE were determined in soil, grapevine, and air biomonitors (moss bags) using ICP-OES and ICP-MS. Environmental implication assessment of soil did not show pollution by PTE, except for B in samples collected in the middle of grapevine season (July). Despite low total Cd concentrations in soil, it has the highest influence on increase of environmental risk. Based on biological accumulation concentration (BAC), grapevine is not hyperaccumulator of PTE from soil. Advanced classification algorithm, Kohonen self-organizing map (SOM), was applied to compare environmental implications in organic with conventional vineyards. PTE concentrations were significantly lower in organic than conventional grapevine. PTE concentrations were higher in the outer (leaf and petiole) than in the inner grapevine parts (skin, pulp, and seed). Some airborne elements have an influence on outer grapevine parts, especially on leaves (ratio factor—RF > 1). Moss bag technique testified about lower enrichment of airborne elements compared with the conventional vineyard and urban microenvironments. Environmental and health risk assessments confirmed that organic production is harmless for field workers and grape consumers.