Habitat loss and fragmentation together represent the most significant threat to the world's biodiversity. In order to guarantee the survival of this diversity, the monitoring of bioindicators can provide important insights into the health of a natural environment. In this context, we used the comet assay and micronucleus test to evaluate the genotoxic susceptibility of 126 bats of eight species captured in soybean and sugarcane plantation areas, together with a control area (conservation unit) in the Cerrado savanna of central Brazil. No significant differences were found between the specimens captured in the sugarcane and control areas in the frequency of micronuclei and DNA damage (comet assay). However, the omnivore Phyllostomus hastatus had a higher frequency of nuclear abnormalities than the frugivore Carollia perspicillata in the sugarcane area. Insectivorous and frugivorous bats presented a higher frequency of genotoxic damage than the nectarivores in the soybean area. In general, DNA damage and micronuclei were significantly more frequent in agricultural environments than in the control area. While agricultural development is an economic necessity in developing countries, the impacts on the natural landscape may result in genotoxic damage to the local fauna, such as bats. Over the medium to long term, then DNA damage may have an increasingly negative impact on the wellbeing of the local species.

Marine Protected Areas (MPAs) are designated to enable the management of damaging activities within a discrete spatial area, and can be effective at reducing the associated impacts, including habitat loss and over-exploitation. Such sites, however, may be exposed to the potential impacts from broader scale pressures, such as anthropogenic litter, due to its diffuse nature and lack of constraint by legislative and/or political boundaries. Plastic, a large component of litter, is of particular concern, due to increasing evidence of its potential to cause ecological and socio-economic damage. The presence of sensitive marine features may mean that some MPAs are at greater potential risk from the impacts of plastic pollution than some non-protected sites. Understanding the abundance, distribution and composition of litter along coastlines is important for designing and implementing effective management strategies. Gathering such data, however, can be expensive and time-consuming but litter survey programmes that enlist citizen scientists are often able to resolve many of the logistical or financial constraints. Here, we examine data collected over 25-years (1994–2018), by Marine Conservation Society volunteers, for spatial patterns in relation to the English MPA network, with the aim of highlighting key sources of litter and identifying management priority areas. We found that MPAs in southeast (Kent) and southwest (Cornwall and Devon) England have the highest densities of shore-based litter. Plastic is the main material constituent and public littering the most common identifiable source. Items attributed to fishing activities were most prevalent in southwest MPAs and sewage related debris was highest in MPAs near large rivers and estuaries, indicating localised accumulation. When comparing inside and outside of MPAs, we found no difference in litter density, demonstrating the need for wider policy intervention at local, national and international scales to reduce the amount of litter.

Batoids (Chondrichthyes: Batoidea; e.g. stingrays, skates, and guitarfish) comprise more than 55% of elasmobranch taxa and represent ecologically important predators in benthic and pelagic habitats. Although overexploitation and habitat degradation are the two biggest threats to batoid populations, coastal and oceanic pollution is also a pervasive potential threat. In this systematic review, we compile published scientific literature on trace metals and persistent organic pollutants (POPs) contamination in elasmobranch species of the Batoidea superorder and present contamination patterns, exposure effects, and potential human exposure risks to most reported contaminants. We found batoids to accumulate a wide range of trace metals, including mercury (Hg), arsenic (As), lead (Pb), copper (Cu), cadmium (Cd) and zinc (Zn). Accumulation of POPs is reported for chlordanes, dichlorodiphenyltrichloroethane (DDT), polychlorinated biphenyl (PCB), dieldrin, Heptachlor epoxide, hexachlorobenzene and perfluoroalkyl substances (PFAS). Hg levels in muscle tissue were significantly different among oceanic basins and habitats, consistent with previous global assessments of Hg oceanic background levels. Some batoid species presented Hg levels higher than large pelagic teleost fishes and comparable to sharks. Ecological traits such as, bottom feeding, upper trophic position and elasmobranch-specific physiology and metabolism are discussed as potential factors associated with Hg uptake and accumulation in batoids. Some species exceeded USEPA's maximum contamination safety limits in edible tissues for Hg, As and ΣPCBs. For most trace metals and POPs, there is a lack of studies focusing on contamination levels in batoids. We recommend future research increasing reporting on POPs and trace metals besides Hg in batoids to further investigate the role of Elasmobranch as a bioindicator for marine pollution.

Lange's metalmark butterfly, Apodemia mormo langei Comstock, is in danger of extinction due to loss of habitat caused by invasive exotic plants which are eliminating its food, naked stem buckwheat. Herbicides are being used to remove invasive weeds from the dunes; however, little is known about the potential effects of herbicides on butterflies. To address this concern we evaluated potential toxic effects of three herbicides on Behr's metalmark, a close relative of Lange's metalmark. First instars were exposed to recommended field rates of triclopyr, sethoxydim, and imazapyr. Life history parameters were recorded after exposure. These herbicides reduced the number of adults that emerged from pupation (24–36%). Each herbicide has a different mode of action. Therefore, we speculate that effects are due to inert ingredients or indirect effects on food plant quality. If these herbicides act the same in A. mormo langei, they may contribute to the decline of this species.

Modification of nighttime light levels by artificial illumination (artificial light at night; ALAN) is a rapidly increasing form of human disturbance that affects natural environments worldwide. Light in natural environments influences a variety of physiological and ecological processes directly and indirectly and, as a result, the effects of light pollution on species, communities and ecosystems are emerging as significant. Small prey species may be particularly susceptible to ALAN as it makes them more conspicuous and thus more vulnerable to predation by visually oriented predators. Understanding the effects of disturbance like ALAN is especially important for threatened or endangered species as impacts have the potential to impede recovery, but due to low population numbers inherent to at-risk species, disturbance is rarely studied. The endangered Stephens’ kangaroo rat (SKR), Dipodomys stephensi, is a nocturnal rodent threatened by habitat destruction from urban expansion. The degree to which ALAN impacts their recovery is unknown. In this study, we examined the effects of ALAN on SKR foraging decisions across a gradient of light intensity for two types of ALAN, flood and bug lights (756 vs 300 lumen, respectfully) during full and new moon conditions. We found that ALAN decreased probability of resource patch depletion compared to controls. Moreover, lunar illumination, distance from the light source and light type interacted to alter SKR foraging. Under the new moon, SKR were consistently more likely to deplete patches under control conditions, but there was an increasing probability of patch depletion with distance from the source of artificial light. The full moon dampened SKR foraging activity and the effect of artificial lights. Our study underscores that ALAN reduces habitat suitability, and raises the possibility that ALAN may impede the recovery of at-risk nocturnal rodents.

In the last decades a dramatic loss of Apis mellifera hives has been reported in both Europe and USA. Research in this field is oriented towards identifying a synergy of contributing factors, i.e. pathogens, pesticides, habitat loss and pollution to the weakening of the hive. Cadmium (Cd) is a hazardous anthropogenic pollutant whose effects are proving to be increasingly lethal. Among the multiple damages related to Cd contamination, some studies report that it causes immunosuppression in various animal species. The aim of this paper is to determine whether contamination by Cd, may have a similar effect on the honey bees’ immunocompetence. Our results, obtained by immune challenge experiments and confirmed by structural and ultrastructural observations show that such metal causes a reduction in immunocompetence in 3 days Cd exposed bees. As further evidence of honey bee response to Cd treatment, Energy Dispersive X-ray Spectroscopy (X-EDS) has revealed the presence of zinc (Zn) in peculiar electron-dense granules in fat body cells. Zn is a characteristic component of metallothioneins (MTs), which are usually synthesized as anti-oxidant and scavenger tools against Cd contamination. Our findings suggest that honey bee colonies may have a weakened immune system in Cd polluted areas, resulting in a decreased ability in dealing with pathogens.

Habitat loss and environmental pollution are among the main causes responsible for worldwide biodiversity loss. The resulting species and population declines affect all vertebrates including reptiles. Especially in industrialized countries, pollution by agrochemicals is of remarkable importance. Here, habitat loss has historically been associated with expansion of agriculture. Species persisting in such environments do not only need to cope with habitat loss, but more recently, also with chemical intensification, namely pesticide exposure. In this study, we examined effects of different fungicide and herbicide applications on the common wall lizard (Podarcis muralis) in grape-growing areas. We used three enzymatic biomarkers (GST, GR, AChE) and for the first time saliva from buccal swabs as a minimal-invasive sampling method for detection. Our results demonstrate absorption of substances by lizards and effects of pesticide exposure on enzymatic activities. Our findings are in accordance with those of previous laboratory studies, although samples were retrieved from natural habitats. We conclude that buccal swabs could become a useful tool for the detection of pesticide exposure in reptiles and have the potential to replace more invasive methods, such as organ extraction or cardiac puncture. This is an important finding, as reptiles are non-target organisms of pesticide applications, and there is a strong need to integrate them into pesticide risk assessments.

Agricultural pesticides continue to impair surface water ecosystems, although there are few assessments of interactions with other modifications such as fine sediment and physical alteration for flood drainage. We, therefore, surveyed pesticide contamination and macroinvertebrates in 14 streams along a gradient of expected pesticide exposure using a paired-reach approach to differentiate effects between physically modified and less modified sites. Apparent pesticides effects on the relative abundance of SPEcies At Risk (SPEAR) were increased at sites with degraded habitats primarily due to the absence of species with specific preferences for hard substrates. Our findings highlight the importance of physical habitat degradation in the assessment and mitigation of pesticide risk in agricultural streams.

Changes to larval fish assemblages may have far reaching ecological impacts. Correlations between habitat modification, contamination and marine larval fish communities have rarely been assessed in situ. We investigated links between the large-scale distribution of stressors and larval fish assemblages in estuarine environments. Larval fish communities were sampled using a benthic sled within the inner and outer zones of three heavily modified and three relatively unmodified estuaries. Larval abundances were significantly greater in modified estuaries, and there were trends towards greater diversity in these systems. Differences in larval community composition were strongly related to sediment metal levels and reduced seagrass cover. The differences observed were driven by two abundant species, Paedogobius kimurai and Ambassis jacksoniensis, which occurred in large numbers almost exclusively in highly contaminated and pristine locations respectively. These findings suggest that contamination and habitat alteration manifest in substantial differences in the composition of estuarine larval fish assemblages.

Ecological restoration is an important tool to reverse habitat loss and recover ecosystem services. Here, for two years, we examine the dynamic of Posidonia oceanica following the restoration of a 1149 m² meadow damaged by the Concordia shipwreck. To evaluate the suitability of a recently employed seagrass restoration protocol, we assessed the patches' survival and development by high-spatial resolution photomosaics over the whole transplanted surface. To estimate recovery trajectories, we quantified the cuttings' survival, shoot density, and Daily Leaf Production within fixed monitoring squares. The outcomes confirmed that our protocol could be efficiently applied at larger scales, showing diminutions in cuttings' survival and shoot density over the first year (up to −20%), followed by stability in the number of living cuttings and increases of leaf bundles (up to +5%/year). Our insights demonstrate that the recovery of P. oceanica can be speeded up and underline the need for case-specific transplantation strategies.