Triclosan (TCS) is an organic compound with a wide range of antibiotic activity and has been widely used in items ranging from hygiene products to cosmetics; however, recent studies suggest that it has several adverse effects. In particular, TCS can be passed to both fetus and infants, and while some evidence suggests in vitro neurotoxicity, there are currently few studies concerning the mechanisms of TCS-induced developmental neurotoxicity. Therefore, this study aimed to clarify the effect of TCS on neural development using zebrafish models, by analyzing the morphological changes, the alterations observed in fluorescence using HuC-GFP and Olig2-dsRED transgenic zebrafish models, and neurodevelopmental gene expression. TCS exposure decreased the body length, head size, and eye size in a concentration-dependent manner in zebrafish embryos. It increased apoptosis in the central nervous system (CNS) and particularly affected the structure of the CNS, resulting in decreased synaptic density and shortened axon length. In addition, it significantly up-regulated the expression of genes related to axon extension and synapse formation such as α1-Tubulin and Gap43, while decreasing Gfap and Mbp related to axon guidance, myelination and maintenance. Collectively, these changes indicate that exposure to TCS during neurodevelopment, especially during axonogenesis, is toxic. This is the first study to demonstrate the toxicity of TCS during neurogenesis, and suggests a possible mechanism underlying the neurotoxic effects of TCS in developing vertebrates.

The severe and pervasive effects of multispecies foodborne microbial biofilms highlight the importance of rapid detection and diagnosis of contamination risk in the field using epifluorescence-based techniques (EBT) combined with automatic image-counting software. This study screened the hygiene quality of the environment, the carcass and the slaughtering equipment in the El-Kharga abattoir, New Valley Province, Egypt, to assess possible contamination during slaughter process. In addition, biofilm was assessed, and bacteria was enumerated by epifluorescence microscopy. Using both conventional and EBT, the highest bacterial counts were observed for the slaughtering equipment (6.6 and 5.2 cfu/cm2, respectively), followed by different parts of the carcass (4.1 and 4.4 cfu/cm2, respectively) and environmental samples (3.9 and 4.1 cfu/cm2, respectively). A high prevalence of E. coli O157:H7 was observed on the slaughtering equipment (25%), which also led to carcass (1%) contamination. Moreover, Enterobacteriaceae members were detected during examination, such as Klebsiella pneumoniae, Enterobacter aerogenes, and Raoultella ornithinolytica. Despite the relatively good hygiene quality of the abattoir environment, there is also a high risk associated with biofilm formation by pathogenic microorganisms on the slaughtering equipment. Moreover, EBT showed different structures of the biofilm, including those formed at different maturation stages, such as voids, microbubbles, channels and mushroom shapes. (EBT) microscopy combined with image-counting software could be a candidate substitute to estimate efficiently, precisely and rapidly the microbial aggregation and exposure risk in field than the conventional counting techniques.

The aim of this study was to examine the effects of arbuscular mycorrhizal (AM) fungi, biochar (BC) addition and phosphorus (P) fertilizer applications on the mycorrhizal response, biomass and elemental uptake of Trifolium repens in cadmium (Cd)-polluted soils. The results showed that mycorrhizal colonization were significantly decreased by 100 mg P kg⁻¹ fertilizer input. Moreover, AM fungi, BC addition and P fertilizer significantly increased shoot biomass accumulation at all treatments. In the absence of BC, the nitrogen (N), potassium (K), calcium (Ca) and magnesium (Mg) contents in the shoots were not affected by AM fungi after P fertilizer application, but the P content in the shoots significantly increased in response to AM fungi. In the absence of BC, both AM fungi and P fertilizer significantly reduced the Cd concentrations in the plant tissues as well as the soil diethylenetriaminepentaacetic acid (DTPA)-Cd concentration. These results indicated that the translocation factors (TFs) were influenced only by BC addition and that the roots could accumulate greater amounts of Cd than the shoots. On the basis of the hygienic standard for feed in China, the shoot Cd concentration in white clover was below the maximum permitted Cd concentration (1 μg g⁻¹) across all treatments. Therefore, it is suggested that no negative mycorrhizal-white clover symbiotic relationships were observed and T. repens could be a suitable forage species for planting in soils with low concentrations of Cd contamination when BC and P fertilizer are applied.

The existence of microplastics (MPs) in canned fish (tuna and mackerel) samples was investigated and their composition, possible sources and potential intake were assessed. Light and fluorescence microscopy were used for the quantification of potential MPs. Furthermore, micro-Raman microscopy, and scanning electron microscopy coupled with an energy dispersive X-ray were used to identify the polymer types and composition of MPs. The results showed that 80% of samples had at least one plastic particle and fibers were the most abundant shapes of MPs. Moreover, polyethylene terephthalate (32.8%) was the most common polymer type in canned fish samples. The fish, food additives, and contact materials during the cleaning and canning process are possible sources of MPs. Human intake estimation of MPs showed the possibility of plastics absorption by humans who consume canned fish several times/week. Hence, the results of this study showed the importance of MPs' guidelines for food safety and hygiene.

This paper reexamines policies and outcomes concerning the NY Bight Restoration Plan, and the NY–NJ Harbor Estuary Program (NYNJHEP) precipitated by washups of marine debris and medical wastes in the New York Bight some 25-years ago. Findings indicate progress has been made but further work is necessary. Extensive beach closures have not occurred since 1987–88, although localized closings occur annually from pathogens. Objectives of “0” beach closures may not be feasible for some beaches, not to exceed 5% closures may be more achievable. Pathogen and DO data show further reductions of the last 10–20% will be more challenging and costly, suggesting “hot spots” be a focus for further remediation. Marine debris show increasing trends on beaches; presence of balloons, plastic bags, syringes and personal hygiene items found annually is another concern. Future challenges are on two fronts, upstream (harbor estuary based)-toxics, nutrient/organic loads, and atmospheric (bight based)-toxics, metals.

Environmental pollution has become more diversified in recent years as technologies for urbanization is increasingly more advanced. Several environmental factors such as air and water pollutants have been linked to allergic symptoms. For instance, because of industrialization for city development in many countries, polluted soil or tiny particles in the air could result in an even more hazardous environment for people to reside. Aside from the aspects of environmental issues, other newly emerging factors such as the electromagnetic field (EMF) also require further investigation. Here, in this narrative review, we focused on allergens from atmospheric and water pollution, hygiene improvement, changes in food trend, and residential environmental pollution. Current evidences regarding the association between various pollutants and the potential clinical diseases could be induced. For people with high skin exposure to air pollutants such as PM 2.5, PM 10, or sulfur dioxide, potential onset of dermatological allergic events should be alerted. The mechanisms involved in allergic diseases are being discussed and summarized. Interactions between immunological mechanisms and clinical implications could potentially provide clearer view to the association between allergic status and pollutants. Moreover, understanding the mechanistic role of allergens can raise awareness to global environment and public health.

Agricultural and livestock activities strongly influence groundwater quality and conditioning its use as water supply in rural areas. The aim was to determine the quality and suitability of the groundwater supply used in dairy farms of an agricultural area of Pampa plain of Córdoba (Argentina). Piper’s diagram showed that the groundwater types were sodium bicarbonate, sodium bicarbonate-chloride, sodium chloride-sulfate, and sodium sulfate. Physicochemical parameters revealed that cations and anions showed a high and significant correlation in water samples, indicating a strong water–rock interaction. Nitrate (NO₃⁻) content was significantly correlated with pH, water well depth, and distance from contamination sources. A high positive correlation between arsenic (As) and bicarbonate, sulfate, sodium, and chloride (p < 0.05) indicates a similar origin. Among the pesticides monitored, 2,4-D was detected in 25% of groundwater samples (0.4 to 0.8 µg/L) coinciding with the ordinary application practices. In general, most of the groundwater samples did not comply with national and international regulations for drinking water and dairy hygiene, due to the high content of As, NO₃⁻, bacteria, and the presence of 2,4-D herbicide. However, the quality of water was suitable for livestock drink. The data obtained in this study contribute to a better understanding of the contamination processes taking place and improve the agricultural and livestock management for an efficient use of this resource by dairy farmers.