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Relative proportions of polycyclic aromatic hydrocarbons differ between accumulation bioassays and chemical methods to predict bioavailability
2010
Gomez-Eyles, Jose L. | Collins, Chris D. | Hodson, Mark E.
Chemical methods to predict the bioavailable fraction of organic contaminants are usually validated in the literature by comparison with established bioassays. A soil spiked with polycyclic aromatic hydrocarbons (PAHs) was aged over six months and subjected to butanol, cyclodextrin and tenax extractions as well as an exhaustive extraction to determine total PAH concentrations at several time points. Earthworm (Eisenia fetida) and rye grass root (Lolium multiflorum) accumulation bioassays were conducted in parallel. Butanol extractions gave the best relationship with earthworm accumulation (r2 ≤ 0.54, p ≤ 0.01); cyclodextrin, butanol and acetone–hexane extractions all gave good predictions of accumulation in rye grass roots (r2 ≤ 0.86, p ≤ 0.01). However, the profile of the PAHs extracted by the different chemical methods was significantly different (p < 0.01) to that accumulated in the organisms. Biota accumulated a higher proportion of the heavier 4-ringed PAHs. It is concluded that bioaccumulation is a complex process that cannot be predicted by measuring the bioavailable fraction alone. The ability of chemical methods to predict PAH accumulation in Eisenia fetida and Lolium multiflorum was hindered by the varied metabolic fate of the different PAHs within the organisms.
Показать больше [+] Меньше [-]Phytoremediation potential of the novel atrazine tolerant Lolium multiflorum and studies on the mechanisms involved
2009
Merini, Luciano J. | Bobillo, Cecilia | Cuadrado, Virginia | Corach, Daniel | Giulietti, Ana M.
Atrazine impact on human health and the environment have been extensively studied. Phytoremediation emerged as a low cost, environmental friendly biotechnological solution for atrazine pollution in soil and water. In vitro atrazine tolerance assays were performed and Lolium multiflorum was found as a novel tolerant species, able to germinate and grow in the presence of 1 mg kg⁻¹ of the herbicide. L. multiflorum presented 20% higher atrazine removal capacity than the natural attenuation, with high initial degradation rate in microcosms. The mechanisms involved in atrazine tolerance such as mutation in psbA gene, enzymatic detoxification via P₄₅₀ or chemical hydrolysis through benzoxazinones were evaluated. It was demonstrated that atrazine tolerance is conferred by enhanced enzymatic detoxification via P₄₅₀. Due to its atrazine degradation capacity in soil and its agronomical properties, L. multiflorum is a candidate for designing phytoremediation strategies for atrazine contaminated agricultural soils, especially those involving run-off avoiding. Finding of a novel atrazine-tolerant species, as a potential candidate for phytoremediating herbicide-contaminated agriculture soils and elucidation of the mechanisms involved in tolerance.
Показать больше [+] Меньше [-]Expression of alkane monooxygenase (alkB) genes by plant-associated bacteria in the rhizosphere and endosphere of Italian ryegrass (Lolium multiflorum L.) grown in diesel contaminated soil
2009
Andria, Verania | Reichenauer, Thomas G. | Sessitsch, Angela
For phytoremediation of organic contaminants, plants have to host an efficiently degrading microflora. To assess the role of endophytes in alkane degradation, Italian ryegrass was grown in sterile soil with 0, 1 or 2% diesel and inoculated either with an alkane degrading bacterial strain originally derived from the rhizosphere of Italian ryegrass or with an endophyte. We studied plant colonization of these strains as well as the abundance and expression of alkane monooxygenase (alkB) genes in the rhizosphere, shoot and root interior. Results showed that the endophyte strain better colonized the plant, particularly the plant interior, and also showed higher expression of alkB genes suggesting a more efficient degradation of the pollutant. Furthermore, plants inoculated with the endophyte were better able to grow in the presence of diesel. The rhizosphere strain colonized primarily the rhizosphere and showed low alkB gene expression in the plant interior. Bacterial alkane degradation genes are expressed in the rhizosphere and in the plant interior.
Показать больше [+] Меньше [-]Phytoavailability and mechanism of bound PAH residues in filed contaminated soils
2017
Gao, Yanzheng | Hu, Xiaojie | Zhou, Ziyuan | Zhang, Wei | Wang, Yize | Sun, Bingqing
Understanding the phytoavailability of bound residues of polycyclic aromatic hydrocarbons (PAHs) in soils is essential to assessing their environmental fate and risks. This study investigated the release and plant uptake of bound PAH residues (reference to parent compounds) in field contaminated soils after the removal of extractable PAH fractions. Plant pot experiments were performed in a greenhouse using ryegrass (Lolium multiflorum Lam.) to examine the phytoavailablility of bound PAH residues, and microcosm incubation experiments with and without the addition of artificial root exudates (AREs) or oxalic acid were conducted to examine the effect of root exudates on the release of bound PAH residues. PAH accumulation in the ryegrass after a 50-day growth period indicated that bound PAH residues were significantly phytoavailable. The extractable fractions, including the desorbing and non-desorbing fractions, dominated the total PAH concentrations in vegetated soils after 50 days, indicating the transfer of bound PAH residues to the extractable fractions. This transfer was facilitated by root exudates. The addition of AREs and oxalic acid to test soils enhanced the release of bound PAH residues into their extractable fractions, resulting in enhanced phytoavailability of bound PAH residues in soils. This study provided important information regarding environmental fate and risks of bound PAH residues in soils.
Показать больше [+] Меньше [-]Will technological modernization for power generation at an oil refinery diminish the risks from air pollution to the Atlantic Rainforest in Cubatão, SE Brazil?
2015
Nakazato, Ricardo K. | Rinaldi, Mirian C.S. | Domingos, Marisa
We assessed the level of atmospheric contamination by S, N and metals before, during and after the installation of a new thermoelectric plant that provides power to an oil refinery in Cubatão, SE Brazil. We measured the foliar accumulation in Lolium multiflorum “Lema” with the aim of evaluating risks to the Atlantic Rainforest that grows in the region. Al, Co, Cr, Cu, K, N, Ni, S, V and Zn were appropriate markers of the new air contamination profile associated with the modern technology. With the exception of V, the leaf contents of these elements significantly increased between the pre-operation to post-operation phases (Al, Co, N, K, S), or only during the transition phase (Zn, Cu, Cr, Ni), and returned to the previous levels after the total shutdown of the old system. Therefore, the expected environmental gain was not achieved with the installation of the new technology.
Показать больше [+] Меньше [-]Hydrocarbon degradation, plant colonization and gene expression of alkane degradation genes by endophytic Enterobacter ludwigii strains
2011
Yousaf, Sohail | Afzal, Muhammad | Reichenauer, Thomas G. | Brady, Carrie L. | Sessitsch, Angela
The genus Enterobacter comprises a range of beneficial plant-associated bacteria showing plant growth promotion. Enterobacter ludwigii belongs to the Enterobacter cloacae complex and has been reported to include human pathogens but also plant-associated strains with plant beneficial capacities. To assess the role of Enterobacter endophytes in hydrocarbon degradation, plant colonization, abundance and expression of CYP153 genes in different plant compartments, three plant species (Italian ryegrass, birdsfoot trefoil and alfalfa) were grown in sterile soil spiked with 1% diesel and inoculated with three endophytic E. ludwigii strains. Results showed that all strains were capable of hydrocarbon degradation and efficiently colonized the rhizosphere and plant interior. Two strains, ISI10-3 and BRI10-9, showed highest degradation rates of diesel fuel up to 68% and performed best in combination with Italian ryegrass and alfalfa. All strains expressed the CYP153 gene in all plant compartments, indicating an active role in degradation of diesel in association with plants.
Показать больше [+] Меньше [-]Using deuterated PAH amendments to validate chemical extraction methods to predict PAH bioavailability in soils
2011
Gomez-Eyles, Jose L. | Collins, Chris D. | Hodson, Mark E.
Validating chemical methods to predict bioavailable fractions of polycyclic aromatic hydrocarbons (PAHs) by comparison with accumulation bioassays is problematic. Concentrations accumulated in soil organisms not only depend on the bioavailable fraction but also on contaminant properties. A historically contaminated soil was freshly spiked with deuterated PAHs (dPAHs). dPAHs have a similar fate to their respective undeuterated analogues, so chemical methods that give good indications of bioavailability should extract the fresh more readily available dPAHs and historic more recalcitrant PAHs in similar proportions to those in which they are accumulated in the tissues of test organisms. Cyclodextrin and butanol extractions predicted the bioavailable fraction for earthworms (Eisenia fetida) and plants (Lolium multiflorum) better than the exhaustive extraction. The PAHs accumulated by earthworms had a larger dPAH:PAH ratio than that predicted by chemical methods. The isotope ratio method described here provides an effective way of evaluating other chemical methods to predict bioavailability.
Показать больше [+] Меньше [-]Biomonitoring of airborne fluoride and polycyclic aromatic hydrocarbons in industrial areas of Cordoba, Argentina, using standardized grass cultures of Lolium multiflorum
2015
Judith Hebelen, Rodriguez | Eduardo Daniel, Wannaz | Jurgen, Franzaring | Andreas, Klumpp | Andreas, Fangmeier | Maria Luisa, Pignata
A biomonitoring study was performed employing standardized grass cultures. Plants of Lolium multiflorum were exposed at 4 industrial sites over three–month periods in two seasons (dry and rainy) and the biomass produced was used for subsequent measurements of fluoride, polycyclic aromatic hydrocarbons (phenanthrene, anthracene, pyrene, benzo[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, dibenzo[a,h]–anthracene and benzo[g,h,i]perylene), total chlorophyll, malondialdehyde, water, and sulfur content. The total content of polycyclic aromatic hydrocarbons (PAHs) revealed seasonal variations, with the highest values corresponding to the dry season, although this species showed a high retention capacity of PAHs during rainy season. In addition, sampling sites with high vehicular traffic and metal–mechanical industries were associated with the highest content of PAHs. Furthermore, physiological degradation associated with anthropogenic activities in the sampling sites was observed. Fluoride content in the biomonitor was associated with the production and use of cement, which was higher in the dry season.
Показать больше [+] Меньше [-]Nickel and chromium concentrations in Italian ryegrass exposed to ambient air in urban, suburban and rural areas
2015
Budka, Anna | Borowiak, Klaudia | Kayzer, Dariusz | Hanć, Anetta | Zbierska, Janina | Barałkiewicz, Danuta | Wolna-Maruwka, Agnieszka | Lisiak, Marta
Nickel and chromium accumulation from the air in Italian ryegrass (Lolium multiflorum L.) is presented in this paper. Plants were exposed at five sites varying in environmental characteristics. Four one-month series were performed during the 2011 and 2012 growing seasons. Chromium and nickel concentrations in leaves were analysed after every series and compared to control plants. The lowest levels of both trace elements were found in samples collected from control sites. Canonical variate analysis revealed differences among sites and the control, and on this basis we found the lowest values in the suburban area. The lowest trace element concentrations of Cr in leaves were observed at the beginning of the growing season and the highest from mid-June to mid-August, while Ni concentrations varied among series and years. Accumulation of both trace elements in Italian ryegrass was at a comparable level or lower than results obtained in similar investigations in other countries. Moreover, the higher concentrations of both elements were probably connected with increased traffic and elevated small industry activities in urban and rural areas.
Показать больше [+] Меньше [-]Effect of liming on plant accumulation of cadmium under upland or flooded conditions
1993
Xiong, L.M. | Lu, R.K. (Institute of Soil Science, Academia Sinica, Nanying (China))