The research of benthic fauna was done in part of the River Temstica, the right tributary of Nisava. Sampling was conducted on four sites, between 10 and 18 July 2010. Quantitative and qualitative analysis revealed the presence of eight groups of macroinvertebrates. The most frequent groups were Diptera, Ephemeroptera, Plecoptera, Trichoptera. Lower presence was shown by the groups of Coleoptera, Chironomidae, Turbellaria, Hydracarina. The greatest species diversity was characterized by order Trichoptera, while the largest number of individuals was characterized by order Diptera. There has been stated a 10 new taxa for this aquifer, with a new taxonomic group – Hydracarina. It is necessary to conduct further research of this river, with a need to analyze more samples from several locations (to conduct a research of the entire river, including the river mouth), and through the annual seasonal dynamics.

Research macroinvertebrates in the lower reaches of the River Veternica was conducted during 2008 in spring and summer season on nine sites, in period of reduced outflow of water from the Lake Barje, when the water level is often reduced to the biological minimum. The presence of 91 taxa from 13 groups of macrozoobenthos was detected. Groups of Diptera, Ephemeroptera and Trichoptera were presented with 18, while Megaloptera, Plesoptera and Nematomorpha with one taxon each. The spring has been allocated 69 and 70 taxa in summer. The average was separated 17 taxa. Average number of macrozoobenthos was 1440 ind/square m per site. The few types of macroinvertebrates were very abundant at the site before the river confluence into the South Morava River. Insects made up 66.4% of the total number of individuals, especially the numerous groups of Diptera with 45.7%, while the share of the group Aquatic amounted 31%. The largest shares of the total abundance of taxa were as follows: Chironomus thumma with 28.16%, Limnedrilus hoffmeisteri with 11.2% and Tubifex tubifex with 6.89% and Caenis macrura from 5.2%. The structural parameters indicate that diversity of macrozoobenthos reduces in downstream direction, reflecting degradation of ecosystems.

Since the year 2004 the water reservoir Kraljevac has been recognized as a Special nature reserve. Investigations of the water reservoir were done during the autumn (November) of 2009 at three localities. on the basis of the results obtained in this study Kraljevac reservoir water is moderately polluted and correspond to classes 2nd and 2nd-3rd based on physical, chemical, microbiological and hydrobiological parameters.