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Differential effects of biogenic and chemically synthesized silver-nanoparticles application on physiological traits, antioxidative status and californidine content in California poppy (Eschscholzia californica Cham)
2022
Hajian, Mohammad Hossein | Ghorbanpour, Mansour | Abtahi, Faezehossadat | Hadian, Javad
Silver nanoparticles (AgNPs) of both biologically and chemically origins trigger various physiological and metabolic processes through interaction with plant cells, exerting positive, negative and inconsequential effects. However, their impacts on plant systems must be critically investigated to guarantee their safe application in food chain. In this study, the effects of chemically synthesized (synthetic) AgNPs (sAgNPs) and biologically synthesized (biogenic) AgNPs (bAgNPs) on physiological and biochemical features of Eschscholzia californica Cham were evaluated at different concentrations (0, 10, 25, 50 and 100 mg L⁻¹). Plants exposed to bAgNPs (at 10 and 25 mg L⁻¹) and sAgNPs (at 10 mg L⁻¹) displayed relatively uniform deposition of AgNPs on leaf surface, however, the higher concentration (100 mg L⁻¹) was accompanied by aggregation of AgNPs, resulting in anatomical and physiological disorders. Foliar application of both AgNPs at lower concentrations resulted in significant (P < 0.01) improve in the content of photosynthetic pigments (chlorophylls a, b, a+b, and carotenoids) and total phenolics over the control in a dose-related manner. Leaf relative water content decreased steadily with increasing both sAgNPs and bAgNPs concentrations-with sAgNPs being more inhibitive. Both types of AgNPs at 100 mg L⁻¹ significantly (P < 0.05) increased electrolyte leakage index, level of lipid peroxidation product (malondialdehyde), and leaf soluble sugar content when compared to controls. No significant difference was found on cell membrane stability index among the plants exposed to bAgNPs and sAgNPs at the lowest concentration over the control. Californidine content was significantly (P < 0.01, by 45.1%) increased upon all the bAgNPs treatments (with a peak at 25 mg L⁻¹) relative to control. The obtained extracts from plants treated with bAgNPs at lower concentrations revealed a significant induction of antioxidant capacity (based on DPPH˙ free radical scavenging and ferrous ions-chelating activities) with lower IC₅₀ values compared to the other treatments. Conclusively, bAgNPs at lower concentrations are potent elicitors of pharmaceutically active compounds biosynthesis, which enhance physiological efficiency of E. californica, but at higher concentrations bAgNPs are equally toxic as sAgNPs.
Показать больше [+] Меньше [-]The evaluation of endocrine disrupting effects of tert-butylphenols towards estrogenic receptor α, androgen receptor and thyroid hormone receptor β and aquatic toxicities towards freshwater organisms
2018
Wang, Jiaying | Wang, Jingpeng | Liu, Jinsong | Li, Jianzhi | Zhou, Lihong | Zhang, Huanxin | Sun, Jianteng | Zhuang, Shulin
The phenolic compounds have posed public concern for potential threats to human health and ecosystem. Tert-butylphenols (TBPs), as one group of emerging contaminants, showed potential endocrine disrupting effects and aquatic toxicities. In the present study, we detected concentrations of 2,4-DTBP ranging from <0.001 to 0.057 μg/L (detection limit: 0.001 μg/L) in drinking water source from the Qiantang River in East China in April 2016. The endocrine disrupting effects of 2-TBP, 2,4-DTBP and 2,6-DTBP toward human estrogen receptor α (ERα), androgen receptor (AR) and thyroid hormone receptor β (TRβ) were evaluated using human recombinant two-hybrid yeast bioassay. Their aquatic toxicities were investigated with indicator organisms including Photobacterium phosphoreum, Vibrio fischeri and freshwater green alga Chlamydomonas reinhardtii. 2-TBP and 2,4-DTBP exhibited moderate antagonistic effects toward human ERα and AR in a concentration-dependent manner. 2-TBP significantly inhibited the light emission of P. phosphoreum. 2-TBP, 2,4-DTBP and 2,6-DTBP significantly inhibited the growth of C. reinhardtii and reduced the chlorophyll content. Our results suggest the potential adverse effects of TBPs on human health and aquatic organisms. The data will facilitate further risk assessment of TBPs and related contaminants.
Показать больше [+] Меньше [-]Effects of elevated O3 on physiological and biochemical responses in three kinds of trees native to subtropical forest in China during non-growing period
2018
Yu, Hao | Cao, Jixin | Chen, Zhan | Shang, He
Numerous studies have documented the negative effects of ozone (O₃) on tree species in growing season, however, little is done in non-growing season. Three evergreen tree species, Phoebe bournei (Hemsl.) Yang (P. bournei), Machilus pauhoi Kanehira (M. pauhoi) and Taxus chinensis (Pilger) Rehd (T. chinensis), were exposed to non-filtered air, 100 nmol mol⁻¹ O₃ air (E1) and 150 nmol mol⁻¹ O₃ air (E2) in open-top chambers in subtropical China. In the entire period of experiment, O₃ fumigation decreased net photosynthesis rate (Pn) through stomatal limitation during the transition period from growing to non-growing season (TGN), and through non-stomatal limitation during the period of non-growing season (NGS) in all species tested. Meanwhile, O₃ fumigation reduced and delayed the resilience of Pn in all species tested during the transition period from non-growing to growing season (TNG). O₃ fumigation significantly decreased chlorophyll contents during NGS, whereas no obvious injury symptoms were observed till the end of experiment. O₃ fumigation induced increases in levels of malondialdehyde, superoxide dismutase, total phenolics and reduced ascorbic acid, and changes in four plant endogenous hormones as well in all species tested during NGS. During NGS, E1 and E2 reduced Pn by an average of 80.11% in P. bournei, 94.56% in M. pauhoi and 12.57% in T. chinensis, indicating that the O₃ sensitivity was in an order of M. pauhoi > P. bournei > T. chinensis. Overall, O₃ fumigation inhibited carbon fixation in all species tested during NGS. Furthermore, O₃-induced physiological activities also consumed the dry matter. All these suggested that elevated O₃, which is likely to come true during NGS in the future, will adversely affect the accumulation of dry matter and the resilience of Pn during TNG in evergreen tree species, and further inhibit their growth and development in the upcoming growing season.
Показать больше [+] Меньше [-]Characterization and influence of biochars on nitrous oxide emission from agricultural soil
2013
Wang, Zhenyu | Zheng, Hao | Luo, Ye | Deng, Xia | Herbert, Stephen | Xing, Baoshan
Extensive use of biochar to mitigate N2O emission is limited by the lack of understanding on the exact mechanisms altering N2O emissions from biochar-amended soils. Biochars produced from giant reed were characterized and used to investigate their influence on N2O emission. Responses of N2O emission varied with pyrolysis temperature, and the reduction order of N2O emission by biochar (BC) was: BC200 ≈ BC600 > BC500 ≈ BC300 ≈ BC350 > BC400. The reduced emission was attributed to enhanced N immobilization and decreased denitrification in the biochar-amended soils. The remaining polycyclic aromatic hydrocarbons (PAHs) in low-temperature biochars (300–400 °C) played a major role in reducing N2O emission, but not for high-temperature biochars (500–600 °C). Removal of phenolic compounds from low-temperature (200–400 °C) biochars resulted in a surprising reduction of N2O emission, but the mechanism is still unknown. Overall, adding giant reed biochars could reduce N2O evolution from agricultural soil, thus possibly mitigating global warming.
Показать больше [+] Меньше [-]Ecological risk of estrogenic endocrine disrupting chemicals in sewage plant effluent and reclaimed water
2013
Sun, Yan | Huang, Huang | Sun, Ying | Wang, Chao | Shi, Xiao-Lei | Hu, Hong-Ying | Kameya, Takashi | Fujie, Koichi
The long-term ecological risk of micropollutants, especially endocrine disrupting chemicals (EDCs) has threatened reclaimed water quality. In this study, estrogenic activity and ecological risk of eight typical estrogenic EDCs in effluents from sewage plants were evaluated. The estrogenic activity analysis showed that steroidal estrogens had the highest estrogenic activity (ranged from 10−1 to 103 ng-E2/L), phenolic compounds showed weaker estrogenic activity (mainly ranged from 10−3 to 10 ng-E2/L), and phthalate esters were negligible. The ecological risk of the estrogenic EDCs which was characterized by risk quotient ranged from 10−4 to 103, with an order in descending: steroids estrogens, phenolic compounds and phthalate esters. The eight estrogenic EDCs were scored and sorted based on the comparison of the estrogenic activity and the ecological risk, suggesting that 17α-ethynylestradiol (EE2), estrone (E1) and estradiol (E2) should be the priority EDCs to control in municipal sewage plants.
Показать больше [+] Меньше [-]High nitrogen and elevated [CO₂] effects on the growth, defense and photosynthetic performance of two eucalypt species
2012
Novriyanti, Eka | Watanabe, Makoto | Kitao, Mitsutoshi | Utsugi, Hajime | Uemura, Akira | Koike, Takayoshi
Atmospheric nitrogen deposition and [CO₂] are increasing and represent environmental problems. Planting fast-growing species is prospering to moderate these environmental impacts by fixing CO₂. Therefore, we examined the responses of growth, photosynthesis, and defense chemical in leaves of Eucalyptus urophylla (U) and the hybrid of E. deglupta × E. camadulensis (H) to different CO₂ and nitrogen levels. High nitrogen load significantly increased plant growth, leaf N, net photosynthetic rate (Agᵣₒwₜₕ), and photosynthetic water use efficiency (WUE). High CO₂ significantly increased Agᵣₒwₜₕ, photosynthetic nitrogen use efficiency (PNUE) and WUE. Secondary metabolite (SM, i.e. total phenolics and condensed tannin) was specifically altered; as SM of U increased by high N load but not by elevated [CO₂], and vice versa for SM of H.
Показать больше [+] Меньше [-]Occurrence and behaviour of selected hydrophobic alkylphenolic compounds in the Danube River
2009
Micić, Vesna | Hofmann, Thilo
Six hydrophobic alkylphenolic compounds were investigated for the first time simultaneously in four different matrices in the Danube River. Maximum sediment concentrations were 2.83, 2.10, 0.28, and 0.035 mg kg⁻¹ for nonylphenol, nonylphenol monoethoxylate, nonylphenol diethoxylate and octylphenol. Maximum levels in suspended particulate matter (SPM) were 0.18, 0.12, 0.10, and 0.003 mg kg 1. No correlation between concentrations in SPM and sediments was found. Octylphenol monoethoxylate and octylphenol diethoxylate were recorded only in sediment at one location. In mussels and water only nonylphenol and octylphenol were found. Nonylphenol concentrations in mussels (up to 0.34 mg kg⁻¹) correlate with concentrations found in SPM and indicate a slight bioaccumulation. Concentrations in water were close to the limit of quantification. We assume in situ formation of nonylphenol monoethoxylate and nonylphenol in sediments at some locations. In some cases nonylphenol in sediments exceeded the provisional EU environmental quality standards.
Показать больше [+] Меньше [-]Malus rootstocks affect copper accumulation and tolerance in trees by regulating copper mobility, physiological responses, and gene expression patterns
2021
Wan, Huixue | Yang, Fengying | Zhuang, Xiaolei | Cao, Yanhong | He, Jiali | Li, Huifeng | Qin, Sijun | Lyu, Deguo
We investigated the roles of rootstocks in Cu accumulation and tolerance in Malus plants by grafting ‘Hanfu’ (HF) scions onto M. baccata (Mb) and M. prunifolia (Mp) rootstocks, which have different Cu tolerances. The grafts were exposed to basal or excess Cu for 20 d. Excess Cu-treated HF/Mb had less biomass, and pronounced root architecture deformation and leaf ultrastructure damage than excess Cu-challenged HF/Mp. Root Cu concentrations and bio-concentration factor (BCF) were higher in HF/Mp than HF/Mb, whereas HF/Mb had higher stem and leaf Cu concentrations than HF/Mp. Excess Cu lowered root and aerial tissue BCF and translocation factor (Tf) in all plants; however, Tf was markedly higher in HF/Mb than in HF/Mp. The subcellular distribution of Cu in the roots and leaves indicated that excess Cu treatments increased Cu fixation in the root cell walls, which decreased Cu mobility. Compared to HF/Mb, HF/Mp sequestered more Cu in its root cell walls and less Cu in leaf plastids, nuclei, and mitochondria. Moreover, HF/Mp roots and leaves had higher concentrations of water-insoluble Cu compounds than HF/Mb, which reduced Cu mobility and toxicity. Fourier transform infrared spectroscopy analysis showed that the carboxyl, hydroxyl and acylamino groups of the cellulose, hemicellulose, pectin and proteins were the main Cu binding sites in the root cell walls. Excess Cu-induced superoxide anion and malondialdehyde were 28.6% and 5.1% lower, but soluble phenolics, ascorbate and glutathione were 10.5%, 41.9% and 17.7% higher in HF/Mp than HF/Mb leaves. Compared with HF/Mb, certain genes involved in Cu transport were downregulated, while other genes involved in detoxification were upregulated in HF/Mp roots and leaves. Our results show that Mp inhibited Cu translocation and mitigated Cu toxicity in Malus scions by regulating Cu mobility, antioxidant defense mechanisms, and transcription of key genes involved in Cu translocation and detoxification.
Показать больше [+] Меньше [-]Enhancement of polyphenolic metabolism as an adaptive response of lettuce (Lactuca sativa) roots to aluminum stress
2020
Chen, Yao | Huang, Lin | Liang, Xin | Dai, Peibin | Zhang, Yuxue | Li, Baohai | Lin, Xianyong | Sun, Chengliang
Polyphenols, pivotal secondary metabolites, are involved in plant adaption to abiotic stresses. Here, we investigated the role and metabolism profile of polyphenols under aluminum (Al) stress in different lettuce genotypes grown in 0.5 mM CaCl₂ solution with AlCl₃ (pH = 4.5). The complementary use of high-resolution mass spectrometry and quantitative biochemical approaches allowed the characterization of total and unique phenols, as well as their roles in Al tolerance. By comparing the most tolerant and sensitive genotype, 8 polyphenols, including 4 phenolic acids, 2 flavonoids, 1 xanthone and 1 unknown compound, were identified in the roots of the tolerant genotype. The total phenolic and flavonoid contents significantly increased in the tolerant genotype under Al stress. Seedlings with more phenolic accumulation usually performed greater Al tolerance. Meanwhile, principal enzymes related to phenolic biosynthesis significantly increased in roots of the tolerance genotype after Al treatment, with phenylalanine ammonia lyase (PAL), cinnamate 4-hydroxylase, and 4-coumarate coenzyme A ligase increased by 16, 18 and 30%, respectively. The elevated total phenolics were significantly suppressed by AIP, a highly specific PAL inhibitor. Consequently, the antioxidant capacity was inhibited, leading to lettuce sensitivity to Al stress. These results clearly suggested the enhancement of unique polyphenolic biosynthesis as an adaptive strategy of lettuce to Al stress by protecting plants from oxidative stress.
Показать больше [+] Меньше [-]De novo transcriptomic analysis predicts the effects of phenolic compounds in Ba River on the liver of female sharpbelly (Hemiculter lucidus)
2020
Guo, J. (Jiahua) | Mo, Jiezhang | Zhao, Qian | Han, Qizhi | Kanerva, Mirella | Iwata, Hisato | Li, Qi
This work aimed at predicting the toxic effects of phenolic compounds in Ba River on the health of female sharpbelly (Hemiculter lucidus) by the de novo transcriptomic analysis of the liver. Sharpbelly, a native fish living in freshwater ecosystem of East Asia, were sampled upstream, near, and downstream of a wastewater discharge to the Ba river. Based on the occurrence of bisphenol A (BPA), nonylphenol (NP), and 4-tert-octylphenol (4-t-OP) in the water and fish sampled from each site, up-, mid-, and down-stream were interpreted as control, high, and low treatment groups, respectively. In the mid-stream group the Fulton’s condition factor (CF) and body weight were remarkably increased by approximate 20%; the gonado-somatic index (GSI) and hepatosomatic index (HSI) in mid-stream fish showed a similar increasing trend but lacking of statistical difference. Exposure to wastewater effluent caused 160 and 162 differentially expressed genes (DEGs) in up-mid and down-mid stream groups, respectively. Two sets of DEGs were primarily enriched in the signaling pathways of drug metabolism, endocrine system, cellular process, and lipid metabolism in the mid-stream sharpbelly, which may alter the fish behavior, disrupt the reproductive function, and lead to hypothyroidism, hepatic steatosis, etc. Taken together, our results linked the disrupted signaling pathways with activities of phenolic compounds to predict the potential effects of wastewater effluent on the health of wild fish.
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