Heavy metals, especially Pb (lead), are generally toxic to living things. Pb can contaminate organisms in the water through the food chain. The purpose of this study is to enhance water quality by using Salvinia molesta to phytoremediate Pb-polluted water. This study aims to evaluate the ability of S. molesta as a Pb phytoremediator. We evaluated total protein, free amino acids produced by the plant, and plant growth (dry biomass). S. molesta was grown in a hydroponic system exposed to Pb at dosages of 0, 5, 10, and 15 ppm for 7 and 14 days. Pb level was analyzed using Atomic Absorption Spectrophotometer and amino acids were analyzed using High-Pressure Liquid Chromatography. Data were statistically analyzed using analysis of variance (ANOVA) followed by Tukey’s test (α < 0.05). Results showed a significant change in Pb content in the roots and leaves of Pb-exposed S. molesta Mitch compared to control. In Pb-exposed plants, total protein and amino acids, especially cysteine, were lowered. S. molesta could be used as a Pb phytoremediator due to its high potential to survive Pb exposure and its ability to absorb Pb.