AGRIS - Search

Benzyl Alcohol Dehydrogenase (BADH) is an important enzyme for hydrocarbon degradation, which can oxidize benzyl alcohols to aldehydes, while being capable of catalyzing a reversible reaction by reducing benzaldehyde. BADH is a member of medium chain alcohol dehydrogenases, in which zinc and NAD are essential for enzyme activity. This paper describes the expression, purification, and characterization of recombinant benzyl alcohol dehydrogenase, encoded by xylB gene from Rhodococcus ruber UKMP-5M. The gene has been amplified and cloned into E. coli, and the recombinant plasmid pGEMT-xylB has been digested by NdeI and HindIII to construct plasmid pET28b-xylC and then ligated into E. coli BL21 (DE3), itself induced by 0.3 mM isopropyl β-D-thiogalactoside (IPTG) at 25°C. The expressed BADH has been 38 kDa, and is purified by affinity chromatography, in which the specific activity was 30 U/mg after 17 folds purification, leading to a NAD-dependent enzyme that uses benzyl alcohol as a substrate for enzyme characterization. The final metabolite is benzaldehyde, identified by gas chromatography mass spectrometry (GC-MS). The BADH activity has been 0.7 U/mL and the optimum pH and temperature, 9.5 and 30ºC, respectively. Also the Michaelis constant (Km) and maximum velocity (Vmax) have accounted to 705 µM and 1.3 U/mL, respectively. Benzyl alcohol dehydrogenase from R. ruber UKMP-5M can be used for hydrocarbon biodegradation in contaminated sites.

Показать больше [+]

purification alcohol dehydrogenase recombinant protein expression

University of Tehran - Iran (Islamic Republic of)

Overhauling health effects perspectives.

1988

Schwebach G.H. | Cafaro D. | Egan J. | Grimes M. | Michael G.

drinking water water water use eau potable agua de riego agua potable purificacion sante publique utilisation de l' eau recyclage des dechets eau d' irrigation salud publica ...

Wolters Kluwer - United States of America

Water pollution | Nonindustrial wastewater pollution can be better managed

1991

auditing sewage water purification pollution united states government policy

National Agricultural Library - United States of America

Water pollution | State revolving funds insufficient to meet wastewater treatment needs | State revolving fund program

1992

sewage evaluation purification united states government policy law and legislation

National Agricultural Library - United States of America

Decontamination and energetic supply in rural installations

1992

Carbonell, V. (Escuela Tecnica Superior de Ingenieros Agronomos, Madrid (Spain). Dept. de Fisica) | Bigeriego, M. | Delgado, M.

eficacia efficiency digestores filtre lisier anaerobiosis biogas/ swine estiercol fluido efficacite purificacion fermentacion biogas/ cerdo ...

Food and Agriculture Organization of the United Nations - Italy

Effects of the recirculation on the anaerobic digestion of cow manure in a biofilm reactor

1992

Sanchez Hernandez, E.P. (National Center for Scientific Research, La Habana (Cuba). Environmental Pollution Dept.) | Weiland, P. | Travieso Cordoba, L.