Heavy metal accumulation in agricultural land causes crop production losses worldwide. Metal homeostasis within cells is tightly regulated. However, homeostasis breakdown leads to accumulation of reactive oxygen species (ROS). Overall plant fitness under stressful environment is determined by coordination between roots and shoots. But little is known about organ specific responses to heavy metals, whether it depends on the metal category (redox or non-redox reactive) and if these responses are associated with heavy metal accumulation in each organ or there are driven by other signals. Maize seedlings were subjected to sub-lethal concentrations of four metals (Zn, Ni, Cd and Cu) individually, and were quantified for growth, ABA level, and redox alterations in roots, mature leaves (L1,2) and young leaves (L3,4) at 14 and 21 days after sowing (DAS). The treatments caused significant increase in endogenous metal levels in all organs but to different degrees, where roots showed the highest levels. Biomass was significantly reduced under heavy metal stress. Although old leaves accumulated less heavy metal content than root, the reduction in their biomass (FW) was more pronounced. Metal exposure triggered ABA accumulation and stomatal closure mainly in older leaves, which consequently reduced photosynthesis. Heavy metals induced oxidative stress in the maize organs, but to different degrees. Tocopherols, polyphenols and flavonoids increased specifically in the shoot under Zn, Ni and Cu, while under Cd treatment they played a minor role. Under Cu and Cd stress, superoxide dismutase (SOD) and dehydroascorbate reductase (DHAR) activities were induced in the roots, however ascorbate peroxidase (APX) activity was only increased in the older leaves. Overall, it can be concluded that root and shoot organs specific responses to heavy metal toxicity are not only associated with heavy metal accumulation and they are specialized at the level of antioxidants to cope with.

Nitric oxide (NO) plays a critical role in atmospheric chemistry and also is a precursor of nitrate, which affects particle matter formation and nitrogen deposition. Agricultural soil has been recognized as a main source of atmospheric NO. However, quantifying the NO fluxes emitted from croplands remains a challenge and in situ long-term measurements of NO are still limited. In this study, we used an automated sampling system to measure NO fluxes with a high temporal resolution over two years (April 2017 to March 2019) from a rainfed maize field in the Northeast China. The cumulative annual NO emissions were 8.9 and 2.3 kg N ha⁻¹ in year 1 (April 2017 to March 2018) and year 2 (April 2018 to March 2019), respectively. These interannual differences were largely related to different weather conditions encountered. In year 1, a month-long drought before and after the seeding and fertilizing reduced plant N uptake and dramatically increased soil N concentration. The following moderate rainfalls promoted large amount of NO emissions, which remained high until late September. The NO fluxes in both years showed clearer seasonal patterns, being highest after fertilizer application in summer, and lowest in winter. The seasonal patterns of NO fluxes were mainly controlled by soil available N concentrations and soil temperatures. The contribution of NO fluxes during the spring freeze-thaw in both years was no more than 0.2% of the annual NO budget, indicating that the freeze-thaw effect on agricultural NO emissions was minimal. In addition, with high-resolution monitoring, we found that soil not only act as a NO source but also a sink. Long-term and high-resolution measurements help us better understand the diurnal, seasonal, and annual dynamics of NO emissions, build more accurate models and better estimate global NO budget and develop more effective policy responses to global climate change.

The emission of mercury (Hg) from cropland soil greatly affects the global Hg cycle. Combinations of different crop cultivars and planting densities will result in different light transmittance under canopies, which directly affects the solar and heat radiation flux received by the soil surface below crops. In turn, this might lead to differences in the soil–air total gaseous mercury (TGM) exchange under different cropping patterns. However, soil–air TGM exchange fluxes in croplands under differing canopies have been poorly investigated. Here, a one-year observation of TGM exchange flux was conducted for cropland soils covering five different crop cultivars and three planting densities in North China Plain using the dynamic flux chamber method. The results showed that light transmittance under the canopies was the key control on soil–air TGM exchange fluxes. High light transmittance can enhance soil TGM emission rates and increase the magnitude of diurnal variations in soil–air TGM exchange fluxes. Furthermore, we found that there were piecewise–function relationships (Peak function–constant equation) between light transmittance under the different canopies and the numbers of days after crop sowing. The soil–air TGM exchange fluxes showed a parabolic response to changes in light transmittance under the different canopies. A second-order model was established for the response relationship between soil–air TGM exchange flux and soil Hg concentration, total solar radiation above the canopy, and numbers of days after sowing. The estimated annual average soil–air TGM exchange flux was 5.46 ± 21.69 ng m⁻² h⁻¹ at corn–wheat rotation cropland with 30 cm row spacing using this second-order model. Our results might a data reference and a promising foundation for future model development of soil–air TGM exchange in croplands under different crop cultivars and planting densities.

Uptake of selenium (Se) by plants largely depend on the availability of Se in soil. Soils and plants were sampled four times within 8 weeks of plant growth in pot experiments using four plant species. Sequential extraction and diffusive gradients in thin-films (DGT) method were employed to measure Se concentrations in potted soils in selenite- or selenate-amended soils. Results showed that DGT-measured Se concentrations (CDGT−Se) were generally several folds higher for selenate than selenite amended soils, which were obviously affected by the plant species and the duration of their growth. For example, the folds in soil planted with mustard were 1.49–3.47 and those in soils planted with purple cabbage and broccoli, which grew for 3 and 4 weeks after sowing, were 1.06–2.14 and only 0.15–0.62 after 6 weeks of growth. The selenate-amended soil planted with wheat showed an extremely high CDGT−Se compared with selenite-amended soil, except the last harvest. Furthermore, minimal changes in CDGT−Se and soluble Se(IV) were found in selenite-amended soils during plant growth, whereas significant changes were observed in selenate-amended soils (p < 0.05). Additionally, Se distribution in various fractions of soil remarkably changed; the soils planted with purple cabbage and broccoli showed the most obvious change followed by wheat and mustard. Soluble Se(VI) and exchangeable Se(VI) were likely the major sources of CDGT−Se in selenate-amended soils, and soluble Se(IV) was the possible source of CDGT−Se in selenite-amended soils. In selenate-amended soils, soluble Se(VI) and exchangeable Se(VI) were significantly correlated with Se concentrations in purple cabbage, broccoli, and mustard; in wheat, Se concentration was significantly correlated only with soluble Se(VI) but not with exchangeable Se. CDGT−Se eventually became positively correlated with Se concentrations accumulated by different plants, indicating that DGT is a feasible method in predicting plant uptake of selenate but not of selenite.

This study analyses 1800 years of heavy metal accumulation in a remote alpine lake experiencing long-range atmospheric contamination and additional inputs of Ag from cloud seeding. In comparison to previous work undertaken on peats, lake sediments show limited post-industrial metal enrichment with enrichment factors of Ag: 1.3, Pb: 1.3, Zn: 1.1, Cu: 1.2 compared to Ag: 2.2, Pb: 3.3, Zn: 2.1, Cu: 4.1 for peat. We show this to be the result of substantial fluvial lithogenic flux of metals (92–97% of total metal flux) to the lake. Total annual metal flux to the lake ranges from: Ag: 4–12 ng/cm2/yr to Zn: 3 383–11 313 ng/cm2/yr. As a result, any contribution of cloud seeding to additional enrichment of Ag in lake sediments is considered negligible. Results show that metal enrichment is not necessarily ubiquitous through a landscape. This has implications for predicting the impacts of atmospheric metal pollution to complex environmental systems.

Ailian Bay is an integrated multi-trophic aquaculture bay with approximately 60 years maricultural activities in North China. The floating raft culture of kelp and shellfish is the unique mariculture mode. In this study, the impacts of intensive mariculture activities on seasonal carbon and nutrient dynamics in Ailian Bay were systematically analysed via seasonal surveys between 2015 and 2016. The dissolved inorganic nitrogen and silicon reached the maximum concentrations during summer, which were mainly attributed to the release of shellfish metabolic by-products and their filter-feeding effects on diatoms. Dissolved organic carbon (DOC) concentrations were significantly elevated when kelps were rotting in summer and kelp seeding were occurring in winter. Meanwhile, the fluorescence intensity of humic-like chromophoric dissolved organic matter was relatively high in kelp mariculture zone. As most humic-like DOC are potentially refractory substances, we propose that kelp mariculture would contribute importantly to the increase of refractory DOC pool in oceans.

Forest harvesting activities on peatlands have long been associated with nutrient leaching and deterioration of downstream water quality. This study aims to assess the effect of grass seeding practice on harvested blanket peatlands to immobilize N and reduce its export to water courses. First, a plot-scale field experiment was conducted by seeding with two grass species (Holcus lanatus and Agrostis capillaris) to study the N uptake potential from a harvested area. Secondly, a simulated rainfall experiment was conducted to study the effect of these grasses on reducing N leaching from surface peat using laboratory flume approach. In the end, the role of seeded grasses in removing N from nutrient-rich throughflow water was assessed using simulated overland flow experiment. The results showed that the seeded grasses had the potential to uptake over 30 kg ha⁻¹ of N in the first year after seeding on harvested peatlands, whereas it takes over 2.5 years to establish the same level of N uptake by natural re-vegetation (non-grassed). In the simulated rainfall experiment, the inorganic N (NH₄⁺-N and NO₃⁻-N) leaching in surface runoff from grassed flumes was 72% lower (453 mg m⁻²) than non-grassed flumes (1643 mg m⁻²). In the simulated overland flow experiment, the N retention by grassed flumes was significantly higher (98%) as compared to non-grassed flumes (70%) in the simulated overland flow experiment. Comparatively higher concentrations of NH₄⁺-N and NO₃⁻-N in soil porewaters of non-grassed flumes suggest that this N retention by non-grassed flumes is less sustainable and is likely to be leached in runoff in subsequent flow events. The results from all three experiments in this study suggest that seeded grasses are a major sink of N on harvested blanket peatland forests. Immobilization of N onsite using the grass seeding and mini-buffer practice could be an efficient and a feasible mean of reducing N export from harvested blanket peatland forests in order to protect the sensitive water courses. However, the sustainability of retention and immobilization of N by grasses needs to be studied further in long-term field-scale experiments on multiple peatland sites.

Lead is known to be a highly toxic metal; it is often found in soils with the potential to be incorporated by plants. Here, the bioaccumulation of lead by rapeseed (Brassica napus) from a soil with Pb(II) added just before sowing is studied. The effect on plant organs is also studied at the ontogenetic stages of flowering and physiological maturity. Moreover, the chemical fractionation of Pb in the rhizosphere and bulk soil portions is investigated and related to Pb accumulation in plant organs. B. napus are found to accumulate Pb in its organs: 1.5–19.6 mg kg⁻¹ in roots, 3.3–15.6 mg kg⁻¹ in stems, 0.5–8.6 mg kg⁻¹ in leaves in all treatments, and in grains 1.45 mg kg⁻¹ at physiological maturity and only for the highest Pb dose (200 mg kg⁻¹). Plant biomass reduction was observed to be about 20% at the flowering stage and only for the highest Pb dose. The analysis of metal fractionation in soil shows Pb migration from the bulk soil to the rhizosphere, attributed to concentration gradients created by root intake. Along the time period studied, lead chemical fractionation in soil evolved toward the most stable fractions, which coupled to plant uptake depleted the soluble/exchangeable one (assumed bioavailable).

This study analysed the effectiveness of innovative (basalt meal, brown algae extract) and conventional (barley straw) substances which hypothetically alleviate the inhibiting effect of Cd²⁺ on biochemical properties of soil, with particular regard to the activity of arylsulfatase. An analysis of their potential was carried out based on the activity of arylsulfatase and the number of Pseudomonas sp. determined on the 25th and 50th days of the study. Cd²⁺ was applied in the following doses: 0, 4, 40, 80, 120, 160, 200 mg Cd²⁺ kg⁻¹ of DM soil, in the form of CdCl₂·2.5H₂O. A complex formulation of the issue was obtained from the presentation of biochemical properties using the RS (resistance of soil) index. Cadmium caused permanent adverse effects in the soil environment, inhibiting the activity of arylsulfatase and the yield of spring barley. The consequences of stress connected with increasing Cd²⁺ pollution were intensified by an elongation of the accumulation time of the tested metal in the soil. Chances for regeneration of the soil may be sought, most of all, with the application of straw and, to a lesser degree, with basalt meal. Brown algae did not meet the expectations for its potential. An increase in the studied parameters also resulted from sowing the soil with spring barley.