Bacteria are candidates for the biotransformation of environmental arsenic (As), while As metabolism in bacteria is not yet fully understood. In this study, we sequenced the genome of an As-resistant bacterium strain Stenotrophomonas maltophilia SCSIOOM isolated from the fish gut. After arsenate (As(V)) exposure, S. maltophilia transformed As(V) to organoarsenicals, along with the significant change of the expression of 40 genes, including the upregulation of arsH, arsRBC and betIBA. The heterogeneous expression of arsH and arsRBC increased As resistance of E. coli AW3110 by increasing As efflux and transformation. E. coli AW3110 (pET-betIBA) could transform inorganic As into dimethylarsinate (DMA) and nontoxic arsenobetaine (AsB), which suggested that AsB could be synthesized through the synthetic pathway of its analog-glycine betaine. In addition, the existence of arsRBC, betIBA and arsH reduced the reactive oxygen species (ROS) induced by As exposure. In total, these results demonstrated that S. maltophilia adopted an As metabolism strategy by reducing As accumulation and synthesizing less toxic As species. We first reported the production and potential synthetic pathway of AsB in bacteria, which improved our knowledge of As toxicology in microorganisms.

Cadmium (Cd) contamination in paddy soil becomes increasingly prominent in recent years, which endangers the safe production of food crops. Cd-tolerant endophytes are ideal mediators for decreasing Cd content in rice plants, but their effects on the rice endophytic microbial community and gene expression profile have not yet been well elucidated. In this study, 58 endophytic bacteria from rice seeds were isolated and characterized. Five strains of them were selected based on their potential growth-promoting traits and strong Cd tolerance that could grow well under 4 mM Cd²⁺. By 16S ribosomal RNA (rRNA) identification, these five strains were designated as Enterobacter tabaci R2-7, Pantoea agglomerans R3-3, Stenotrophomonas maltophilia R5-5, Sphingomonas sanguinis R7-3 and Enterobacter tabaci R3-2. Pot experiments in relieving Cd stress in rice plants showed that the S. maltophilia R5-5 performed the strongest potential for reducing the Cd content in root and blade by 81.33% and 77.78%, respectively. The endophytic microbial community diversity, richness and composition were significantly altered in S. maltophilia R5-5 inoculated rice plants. Reverse-transcription qPCR (RT-qPCR) showed that the expression of Cd transporters, OsNramp5 and OsHMA2, were down-regulated in S. maltophilia R5-5-innoculated rice roots. The results indicate that the inoculation of endophytic bacteria S. maltophilia R5-5 provides a reference for alleviating the heavy metal contamination in paddy fields and can be a better alternative for guaranteeing the safe production of crops. Changes in the relative abundance of Cd-resistant microorganisms and the expression of Cd transporters might be the intrinsic factors affecting cadmium content in rice.

Understanding the environmental transformation of multiwall carbon nanotubes (MWCNTs) is important to their life cycle assessment and potential environmental impacts. We report that a bacterial community is capable of degrading 14C-labeled MWCNTs into 14CO2 in the presence of an external carbon source via co-metabolism. Multiple intermediate products were detected, and genotypic characterization revealed three possible microbial degraders: Burkholderia kururiensis, Delftia acidovorans, and Stenotrophomonas maltophilia. This result suggests that microbe/MWCNTs interaction may impact the long-term fate of MWCNTs.

A phenanthrene-degrading bacterial consortium (ASP) was developed using sediment from the Alang–Sosiya shipbreaking yard at Gujarat, India. 16S rRNA gene-based molecular analyses revealed that the bacterial consortium consisted of six bacterial strains: Bacillus sp. ASP1, Pseudomonas sp. ASP2, Stenotrophomonas maltophilia strain ASP3, Staphylococcus sp. ASP4, Geobacillus sp. ASP5 and Alcaligenes sp. ASP6. The consortium was able to degrade 300ppm of phenanthrene and 1000ppm of naphthalene within 120h and 48h, respectively. Tween 80 showed a positive effect on phenanthrene degradation. The consortium was able to consume maximum phenanthrene at the rate of 46mg/h/l and degrade phenanthrene in the presence of other petroleum hydrocarbons. A microcosm study was conducted to test the consortium’s bioremediation potential. Phenanthrene degradation increased from 61% to 94% in sediment bioaugmented with the consortium. Simultaneously, bacterial counts and dehydrogenase activities also increased in the bioaugmented sediment. These results suggest that microbial consortium bioaugmentation may be a promising technology for bioremediation.

Petroleum products spill and leakage have become two major environmental challenges in Iran. Sampling was performed in the petroleum reservoir waste water of Tehran and Kerman Provinces of Iran. Alkane degrading bacteria were isolated by enrichment in a Bushnel–Hass medium, with hexadecane as sole source of carbon and energy. The isolated strains were identified by amplification of 16S rDNA gene and sequencing. Specific primers were used for identification of alkane hydroxylase gene. Fifteen alkane degrading bacteria were isolated and 8 strains were selected as powerful degradative bacteria. These 8 strains relate to Rhodococcus jostii, Stenotrophomonas maltophilia, Achromobacter piechaudii, Tsukamurella tyrosinosolvens, Pseudomonas fluorescens, Rhodococcus erythropolis, Stenotrophomonas maltophilia, Pseudomonas aeruginosa genera. The optimum concentration of hexadecane that allowed high growth was 2.5%. Gas chromatography results show that all strains can degrade approximately half of hexadecane in one week of incubation. All of the strains have alkane hydroxylase gene which are important for biodegradation. As a result, this study indicates that there is a high diversity of degradative bacteria in petroleum reservoir waste water in Iran.

The aim of the present study was to investigate biosurfactant production ability of five different polyaromatic hydrocarbon (PAH)-metabolizing bacteria, such as Ochrobactrum anthropi IITR07, Pseudomonas mendocina IITR46, Microbacterium esteraromaticum IITR47, Pseudomonas aeruginosa IITR48, and Stenotrophomonas maltophilia IITR87. These bacteria showed biosurfactant production using 2% glucose as rich substrate; strain IITR47 yielded the highest with 906 and 534 mg/L biosurfactant in the presence of naphthalene and crude oil as the unique carbon sources. P. aeruginosa IITR48 showed the least surface tension at 29 N/m and the highest emulsification index at 63%. The biosurfactants produced were identified as glycolipid and rhamnolipid based on Fourier transform infrared spectroscopy analysis. In particular, the biosurfactant produced by bacteria S. maltophilia IITR87 efficiently emulsified mustard oil with an E24 value of 56%. It was observed that, all five biosurfactants from these degrader strains removed 2.4-, 1.7-, 0.9-, 3.8-, and 8.3-fold, respectively, crude oil from contaminated cotton cloth. Rhamnolipid derived from IITR87 was most efficient, exhibiting highest desorption of crude oil. These biosurfactants exhibited good stability without significantly losing its emulsification ability under extreme conditions, thus can be employed for bioremediation of PAHs from diverse contaminated ecosystem. Graphical Abstract

Currently, there is a considerable interest on application of bio-based surfactants as an alternative to conventional synthetic ones as well as in bioremediation technologies to decontaminate polluted sites more effectively. The work is focused on the study of the effects of two biosurfactants, non-ionic Saponin and anionic Rhamnolipids R-90 on the biodegradation of Delor 103, the industrial mixture of polychlorinated biphenyls (PCBs) by bioaugmented bacterial strains. The bacterial isolates used in this study were obtained from long-term PCB-contaminated sediments of the industrial waste Strážsky canal. Enhanced biodegradation of PCBs by Gram-negative strains Achromobacter xylosoxidans (93%) and Stenotrophomonas maltophilia (66%) was observed with the addition of (bio)surfactants Saponin, Rhamnolipids R-90, and Triton X-100 in defined liquid mineral media. The addition of biosurfactant Saponin and Rhamnolipids R-90 increased the PCB biodegradation (55 and 60%, respectively) in the bioaugmented PCB-contaminated sediment inoculated with bacterial strain A. xylosoxidans as well. Regarding to the inhibitory effect of used (bio)surfactants, the obtained IC₅₀ values confirmed that the non-ionic phytogenic Saponin and synthetic surfactant Triton X-100 had a significantly lower toxicity toward bioluminescence of the standard bacteria Vibrio fischeri and used PCB-degrading bacterial strains than the anionic bacterial surfactant Rhamnolipids R-90.

This study reports the presence of the blaPER, sul1 and sul2 genes and class 1 integron inside an IncA/C plasmid with ~ 30 Kb in an isolate of Stenotrophomonas maltophilia. PER-producing Stenotrophomonas maltophilia was obtained from a soil sample cultivated with corn in Maringá City, Paraná State, Brazil. To the best of our knowledge, this is the first report in the world of blaPER gene in a bacterium isolated from soil and in a Stenotrophomonas maltophilia.

The fungicide carbendazim is an ecotoxic pollutant frequently found in water reservoirs. The ability of microorganisms to remove pollutants found in diverse environments, soil, water, or air is well documented. Although microbial communities have many advantages in bioremediation processes, in many cases, those with the desired capabilities may be slow-growing or have low pollutant degradation rates. In these cases, the manipulation of the microbial community through enrichment with specialized microbial strains showing high specific growth rates and high rates and efficiencies of pollutant degradation is desirable. In this work, bacteria of the genera Klebsiella, Flavobacterium, and Stenotrophomonas, isolated from the biofilm attached to the packed zones of a biofilm reactor, were able to grow individually in selective medium containing carbendazim. In the three bacteria studied, the mheI gene encoding the first enzyme involved in the degradation of the fungicide carbendazim was found. Studying the dynamics of growth and carbendazim degradation of the three bacteria, the effect of co-formulants was also evaluated. The pure compound and a commercial formulation of carbendazim were used as substrates. Finally, the study made it possible to define the biokinetic advantages of these strains for amendment of microbial communities.

In recent years, the photocatalytic process by using TiO₂ nanoparticles (NPs) has produced a great interest in wastewater treatment due to its interesting features such as low-cost, environmental compatibility, and especially capacity to eliminate persistent organic compounds as well as microorganisms in water. In the present work, the photocatalytic activity of Gd-doped TiO₂ nanopowders, with different doping amount 0.1, 1, and 5 mol% synthesized by the sol-gel method, was studied under UV/Visible irradiation for water treatment application. The Gd-doped TiO₂ nanoparticles were investigated for their photocatalytic degradation of methylene blue (MB) dye and antibacterial activities against two bacterial strains namely Stenotrophomonas maltophilia (S. maltophilia) and Micrococcus luteus (M. luteus). MB dye was used as a pollutant model to estimate reactive oxygen species (ROS) generation and to correlate killing action of nanoparticles with the generation of ROS. Scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), and Raman spectroscopy were used to characterize the as-synthesized nanomaterials. Photocatalytic, as well as antibacterial tests, showed that doping with an appropriate amount of Gd could reduce the radiative recombination process of photogenerated electron-hole pairs in TiO₂ and induce a significant enhancement in photocatalytic and consequently antibacterial activity. The experimental sequence of bactericidal activity and photocatalytic degradation efficiency exhibited by the different gadolinium-doped nanoparticles was the following: 0.1 mol% Gd-doped TiO₂ > 1 mol% Gd-doped TiO₂ > 5 mol% Gd-doped TiO₂ > pure titania.