Antibiotic resistance surveillance data is lacking in many parts of the world, limiting effective therapy and management of resistance development. Analysis of urban wastewater, which contains bacteria from thousands of individuals, opens up possibilities to generate informative surveillance data in a standardized and resource-efficient way. Here, we evaluate the relationship between antibiotic resistance prevalence in E. coli from wastewater and clinical samples by studying countries with different resistance situations as assessed by traditional clinical surveillance. Composite, influent wastewater samples were collected over 24 h from treatment plants serving major cities in ten European countries. Using a broth screening method, resistance to six antibiotic classes was analyzed for 2507 E. coli isolates (n = 247–252 per country). Resistance prevalence in wastewater E. coli was compared to that in clinical E. coli reported by the European Antibiotic Resistance Surveillance Network. Resistance prevalence was lower in wastewater than clinical E. coli but followed similar geographic trends. Significant relationships were found for resistance to aminopenicillins (R² = 0.72, p = 0.0019) and fluoroquinolones (R² = 0.62, p = 0.0072), but not for aminoglycosides (R² = 0.13, p = 0.31) and third-generation cephalosporins (R² = 0.00, p = 0.99) where regression analyses were based on considerably fewer resistant isolates. When all four antibiotic classes were taken into account, the relationship was strong (R² = 0.85, p < 0.0001). Carbapenem resistance was rare in both wastewater and clinical isolates. Wastewater monitoring shows promise as method for generating surveillance data reflecting the clinical prevalence of antibiotic resistant bacteria. Such data may become especially valuable in regions where clinical surveillance is currently limited.

Antimicrobial resistance (AMR) is a recognised threat to global health. Obtaining data on the prevalence of AMR in environmental bacteria is key to understanding drivers and routes of transmission. Here, 325 Shiga toxin negative deer faecal samples—gathered from across the Scottish mainland—were screened for the presence of AMR Escherichia coli and investigated for potential risk factors associated with AMR occurrence. E. coli with resistance to antimicrobials of clinical health concern, including carbapenems and 3rd generation cephalosporins, were targeted. Ninety-nine percent of samples yielded E. coli, and the prevalence of resistant E. coli at the level of faecal samples was 21.8% (n = 71) for tetracycline, 6.5% (n = 21) for cefpodoxime, 0.3% for ciprofloxacin (n = 1), with no recorded resistance to meropenem. Potential risk factors for tetracycline and cefpodoxime resistance were investigated. The presence of broadleaved woodlands was significantly associated with both AMR phenotypes, which may relate to land use within or around such woodlands. Associated risk factors varied across resistance phenotype and deer species, with proximity or density of horses an indicator of significantly decreased and increased risk, respectively, or tetracycline and cefpodoxime resistance in E. coli from roe deer, but not from red deer. Distance from wastewater treatment plants was a significant risk factor for tetracycline resistance in E. coli from red deer but not from roe deer. Data indicated that AMR E. coli can occur in wild deer populations that are not directly exposed to the selective pressure exerted by antimicrobial treatment. Overall, resistance to critically important antimicrobials was found to be low in the studied population, suggesting no immediate cause for concern regarding human health. Utilising existing culling frameworks, wild deer in Scotland could function well as a sentinel species for the surveillance of AMR in the Scottish environment.

Following the objective to provide an overview on the spatial and temporal variation in forest condition on a European scale, an extensive systematic survey on more than 6000 so called Level I plots throughout Europe is carried out. Results show a recuparation of crown condition in the mid nineties and a resuming deterioration with nearly a quarter of the sample trees classified as damaged in 2001. Results of statistical evaluations explain the observed variation mainly by weather extremes, insects, fungi and indirect effects of air pollutants via soil acidification or changes in the nutritional status of trees

Investigation of air pollution impact on tree condition indicates the greatest correlation between pine defoliation and SO2, SO4 and NH4 concentration in the air, NH4 load with precipitation and its pH in the eastern part of Lithuania. In the southern part significant correlation was found between defoliation and SO4 concentration in the air and SO4, NH4 load with precipitation and its pH. No significant correlation was found in the western region of the country, which includes seaside part. Tree age and tree development classes have an essential effect on the correlation significance

Passive samplers offer considerable advantages for measuring ozone (O3) concentrations in remote areas. Measurements with passive samplers typically result in data about weekly-to-monthly O3 concentrations, which are not consistent with the definitions of cumulative indices currently being used to estimate the exposure of vegetation to O3. For this reason, attempts were and are being made to develop statistical and empirical methods to estimate exposure indices like the AOT40 adopted in Europe (O3 Accumulated Over Threshold 40 ppb) starting from mean concentrations obtained from passive samplers. This paper describes an empirical method adopted in Italy to estimate 1996-2000 AOT40 values at 20 forest sites of the Italian network of intensive monitoring of forest ecosystems

The results indicate that it is possible to reach a good level of reproducibility in a relatively short time. However, quality assurance procedures allow to know the real status of reliability of the field data. More attention must be given to the Level II indices, to select the really useful and informative ones, and to concentrate on these the didactic efforts