Extended-spectrum β-lactamases (ESBL)-producing Enterobacterales have been classified as critical priority pathogens by the World Health Organization (WHO). ESBL are universally distributed and, in 2006, were firstly reported on a wild animal. Understanding the relative contributions of wild animals to ESBL circulation in the environment is urgently needed. In this work, we have conducted a nationwide study in Portugal to investigate the occurrence of bacteria carrying clinically significant antimicrobial resistance genes (ARG), using widely distributed wild ungulates as model species. A total of 151 antimicrobial resistant-Enterobacterales isolates were detected from 181 wild ungulates: 50% (44/88) of isolates from wild boar (Sus scrofa), 40.3% (25/62) from red deer (Cervus elaphus), 41.4% (12/29) from fallow deer (Dama dama) and 100% (2/2) from mouflon (Ovis aries subsp. musimon). Selected isolates showed a diversified resistance profile, with particularly high values corresponding to ampicillin (71.5%) and tetracycline (63.6%). Enterobacterales strains carried blaTEM, tetA, tetB, sul2, sul1 or dfrA1 ARG genes. They also carried blaCTX₋M-type genes, which are prevalent in human infections, namely CTX-M-14, CTX-M-15 and CTX-M-98. Strikingly, this is the first report of CTX-M-98 in wildlife. Almost 40% (n = 59) of Enterobacterales were multi-drug resistant. The diversity of plasmids carried by ESBL isolates was remarkable, including IncF, K and P. This study highlights the potential role of wild ungulates as environmental reservoirs of CTX-M ESBL-producing E. coli and in the spill-over of AMR bacteria and their determinants. Our findings suggest that wild ungulates are useful as strategic sentinel species of AMR in terrestrial environments, especially in response to potential sources of anthropogenic pollution, providing early warning of potential risks to human, animal and environmental health.

Extended-spectrum β-lactamases (ESBL)-producing Enterobacterales have been classified as critical priority pathogens by the World Health Organization (WHO). ESBL are universally distributed and, in 2006, were firstly reported on a wild animal. Understanding the relative contributions of wild animals to ESBL circulation in the environment is urgently needed. In this work, we have conducted a nationwide study in Portugal to investigate the occurrence of bacteria carrying clinically significant antimicrobial resistance genes (ARG), using widely distributed wild ungulates as model species. A total of 151 antimicrobial resistant-Enterobacterales isolates were detected from 181 wild ungulates: 50% (44/88) of isolates from wild boar (Sus scrofa), 40.3% (25/62) from red deer (Cervus elaphus), 41.4% (12/29) from fallow deer (Dama dama) and 100% (2/2) from mouflon (Ovis aries subsp. musimon). Selected isolates showed a diversified resistance profile, with particularly high values corresponding to ampicillin (71.5%) and tetracycline (63.6%). Enterobacterales strains carried blaTEM, tetA, tetB, sul2, sul1 or dfrA1 ARG genes. They also carried blaCTX-M-type genes, which are prevalent in human infections, namely CTX-M-14, CTX-M-15 and CTX-M-98. Strikingly, this is the first report of CTX-M-98 in wildlife. Almost 40% (n = 59) of Enterobacterales were multi-drug resistant. The diversity of plasmids carried by ESBL isolates was remarkable, including IncF, K and P. This study highlights the potential role of wild ungulates as environmental reservoirs of CTX-M ESBL-producing E. coli and in the spill-over of AMR bacteria and their determinants. Our findings suggest that wild ungulates are useful as strategic sentinel species of AMR in terrestrial environments, especially in response to potential sources of anthropogenic pollution, providing early warning of potential risks to human, animal and environmental health. | info:eu-repo/semantics/publishedVersion

Contaminant monitoring in tissues of wild species can help in the knowledge not only of their health, but also of the environmental health conditions in the zones where they live. In this study, concentrations of toxic metals (As, Cd, Hg, and Pb) and an essential metal (Zn) were measured in the hair of red deer (Cervus elaphus) and wild boar (Sus scrofa) inhabiting in protected areas of South-Western Spain. Zn (69.02 ± 1.03 mg kg⁻¹, 70.31 ± 2.22 mg kg⁻¹), Pb (0.61 ± 0.05 mg kg⁻¹, 0.68 ± 0.08 mg kg⁻¹), and As (0.33 ± 0.03 mg kg⁻¹, 0.53 ± 0.08 mg kg⁻¹) were detected in the hair of red deer and wild boar, respectively. The other metals (Cd and Hg) were under the quantification limit (0.1 mg kg⁻¹). No differences in the level of elements between both species were found. The levels of the detected elements in the hair showed low concentrations and similar to those observed in animals of the same species from unpolluted zones, reflecting a lack of contamination by these elements in the studied areas. Based on critical values of these elements established for organs of mammals, red deer and wild boar from the studied territories did not suffer toxicosis by the studied elements. Age-related differences in the content of As in the hair of red deer and age- and gender-related differences in the content of As in the hair of wild boar were detected, which should be considered for biomonitoring purposes. Finally, positive (Pb-As in both species) and negative (Zn-Pb in wild boar) interrelationships between elements were found.

Publicado en: Oropesa, AL., Ramos, A. & Gómez, LJ. Toxic and essential metal levels in the hair of red deer (Cervus elaphus) and wild boar (Sus scrofa) for monitoring the contamination in protected areas of South-Western Spain. Environ Sci Pollut Res 29, 27430–27442 (2022). https://doi.org/10.1007/s11356-021-16439-0 | Contaminant monitoring in tissues of wild species can help in the knowledge not only of their health, but also of the environ mental health conditions in the zones where they live. In this study, concentrations of toxic metals (As, Cd, Hg, and Pb) and an essential metal (Zn) were measured in the hair of red deer (Cervus elaphus) and wild boar (Sus scrofa) inhabiting in protected areas of South-Western Spain. Zn (69.02 ± 1.03 mg kg−1 , 70.31 ± 2.22 mg kg−1), Pb (0.61 ± 0.05 mg kg−1, 0.68 ± 0.08 mg kg−1), and As (0.33 ± 0.03 mg kg−1 , 0.53 ± 0.08 mg kg−1) were detected in the hair of red deer and wild boar, respectively. The other metals (Cd and Hg) were under the quantification limit (0.1 mg kg−1). No differences in the level of elements between both species were found. The levels of the detected elements in the hair showed low concentrations and similar to those observed in animals of the same species from unpolluted zones, reflecting a lack of contamination by these elements in the studied areas. Based on critical values of these elements established for organs of mammals, red deer and wild boar from the studied territories did not suffer toxicosis by the studied elements. Age-related differences in the content of As in the hair of red deer and age- and gender-related differences in the content of As in the hair of wild boar were detected, which should be considered for biomon itoring purposes. Finally, positive (Pb-As in both species) and negative (Zn-Pb in wild boar) interrelationships between elements were found. | This research was in part supported by Extremadura Community through Consejería de Economía, Competitividad e Innovación (Project IB16079; role: collection of samples and review of translation of the report) and Consejería de Economía, Ciencia y Agenda Digital (Support GR18080), and FEDER Funds (role: chemical analysis and review of the report translation).

This is the first field study to evaluate lead exposure in dogs fed game meat and offal and, to our knowledge, the first study exploring associations between game consumption and dog health status. We quantified lead concentrations in blood and hair and haematological parameters of 31 dogs fed game meat and offal from wild boar (Sus scrofa) and axis deer (Axis axis) culled with lead ammunition in El Palmar National Park, Argentina. Despite variable weekly frequency in game consumption, dogs had detectable blood and hair lead levels, demonstrating recent and chronic exposure. Lead geometric mean and SD were 18.91 ± 346.85 μg/dL w.w. in blood (range = 0.53–216.58), and 0.721 ± 6.444 μg/gr d.w. in hair (range = 0.007–34.800). Hair lead levels were relatively low in most samples, except for the oldest dog which had an atypically high value concurrent with anaemia, a common outcome of chronic lead exposure. Dog’s owner was significantly predictive of both blood and hair lead levels, which reflects the same feeding patterns for all dogs owned by the same person. Body condition was associated with hair lead, with dogs in good condition presenting higher lead levels. This could be related to greater game consumption by those dogs, resulting in higher lead ingestion. Dogs fed game meat and offal at very low or low frequency (≤4 times per week) showed higher blood lead levels, suggesting there might not be a risk-free frequency for game provision to dogs. Considering the risks of dietary lead exposure, avoiding feeding dogs lead-killed game and replacing lead ammunition with non-toxic alternatives are recommended. This would allow using hunted game as a valuable food resource without unnecessary risk for the health of consumers and the environment.

This is the first field study to evaluate lead exposure in dogs fed game meat and offal and, to our knowledge, the first study exploring associations between game consumption and dog health status. We quantified lead concentrations in blood and hair and haematological parameters of 31 dogs fed game meat and offal from wild boar (Sus scrofa) and axis deer (Axis axis) culled with lead ammunition in El Palmar National Park, Argentina. Despite variable weekly frequency in game consumption, dogs had detectable blood and hair lead levels, demonstrating recent and chronic exposure. Lead geometric mean and SD were 18.91 ± 346.85 μg/dL w.w. in blood (range = 0.53–216.58), and 0.721 ± 6.444 μg/gr d.w. in hair (range = 0.007–34.800). Hair lead levels were relatively low in most samples, except for the oldest dog which had an atypically high value concurrent with anaemia, a common outcome of chronic lead exposure. Dog’s owner was significantly predictive of both blood and hair lead levels, which reflects the same feeding patterns for all dogs owned by the same person. Body condition was associated with hair lead, with dogs in good condition presenting higher lead levels. This could be related to greater game consumption by those dogs, resulting in higher lead ingestion. Dogs fed game meat and offal at very low or low frequency (≤4 times per week) showed higher blood lead levels, suggesting there might not be a risk-free frequency for game provision to dogs. Considering the risks of dietary lead exposure, avoiding feeding dogs lead-killed game and replacing lead ammunition with non-toxic alternatives are recommended. This would allow using hunted game as a valuable food resource without unnecessary risk for the health of consumers and the environment. | Fil: Fernández, Valentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina | Fil: Caselli, Elsa Andrea. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina | Fil: Tammone Santos, Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina | Fil: Condorí, Walter Ezequiel. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina | Fil: Vanstreels, Ralph Eric Thijl. University of California at Davis; Estados Unidos | Fil: Delaloye, Aldo. Administración de Parques Nacionales; Argentina | Fil: Sosa, Cristian. Administración de Parques Nacionales; Argentina | Fil: Uhart, Marcela María. University of California at Davis; Estados Unidos

Wild pigs (Sus scrofa) are widespread across many landscapes throughout the world and are considered to be an invasive pest to agriculture and the environment, or conversely a native or desired game species and resource for hunting. Wild pig population monitoring is often required for a variety of management or research objectives, and many methods and analyses for monitoring abundance are available. Here, we describe monitoring methods that have proven or potential applications to wild pig management. We describe the advantages and disadvantages of methods so that potential users can efficiently consider and identify the option(s) best suited to their combination of objectives, circumstances, and resources. This paper offers guidance to wildlife managers, researchers, and stakeholders considering population monitoring of wild pigs and will help ensure that they can fulfill their monitoring objectives while optimizing their use of resources.

Wild pigs (Sus scrofa) are widespread across many landscapes throughout the world and are considered to be an invasive pest to agriculture and the environment, or conversely a native or desired game species and resource for hunting. Wild pig population monitoring is often required for a variety of management or research objectives, and many methods and analyses for monitoring abundance are available. Here, we describe monitoring methods that have proven or potential applications to wild pig management. We describe the advantages and disadvantages of methods so that potential users can efficiently consider and identify the option(s) best suited to their combination of objectives, circumstances, and resources. This paper offers guidance to wildlife managers, researchers, and stakeholders considering population monitoring of wild pigs and will help ensure that they can fulfill their monitoring objectives while optimizing their use of resources.