The sensitivity of diatom taxonomy and trait-based endpoints to chemicals has been poorly used so far in Environmental Risk Assessment. In this study, diatom assemblages in outdoor flow-through mesocosms were exposed to thiram (35 and 170 μg/L), and a hydrocarbon emulsion (HE; 0.01, 0.4, 2 and 20 mg/L). The effects of exposure were assessed for 12 weeks, including 9 weeks post-treatment, using taxonomic structure and diversity, bioindication indices, biological traits, functional diversity indices, indicator classes and ecological guilds. For both chemicals, diversity increased after the treatment period, and responses of ecological traits were roughly identical with an abundance increase of motile taxa tolerant to organic pollution and decrease of low profile taxa. Bioindication indices were not affected. Traits provided a complementary approach to biomass measurements and taxonomic descriptors, leading to a more comprehensive overview of ecological changes due to organic chemicals, including short- and long-term effects on biofilm structure and functioning.

The photocatalytic activity of semiconducting silver carbonate was restricted by the lower stability and fast recombination rate of photogenerated electron–hole pairs. Sulfur-doped graphene oxide (SGO) is used as a cocatalyst for improving the photocatalytic activity of Ag₂CO₃ by reducing the recombination rate. A simple precipitation method was used for the modification of silver carbonate. The chemical, physical, optical, and electrochemical properties of the modified photocatalyst was characterized by XRD, SEM, TEM, UV–vis DRS, XPS, CV, impedance, and amperometry. The fabricated SGO-Ag₂CO₃ composite was successfully degraded various organic pollutants such as methylene blue (MB), rhodamine B(RhB), methyl orange (MO), tartrazine, and thiram with augmented mineralization. The optimization of weight percentage of the developed binary composite with 0.5% SGO-Ag₂CO₃ showed enhanced photocatalytic degradation and followed pseudo-first-order kinetics with rate constant 0.126. More than 90% of degradation efficiency of the pollutants within a short time promises the binary heterostructure for future industrial applications. The excellent stability and reproducibility of the composite opened a new route in the treatment of wastewater.

Tibial dyschondroplasia (TD) is a common skeletal disease occurred in growth plate of fast-growing broilers. Thiram is a sort of chemical used for pesticide and fungicide. The excessive use of thiram increased the threat to animal and human health. In this study, we aimed to investigate the therapeutic mechanism of chlorogenic acid (CGA) on thiram-induced tibial dyschondroplasia. Broiler chickens were divided into three different groups, e.g., control, TD, and CGA. CGA was administrated after the induction of TD from 4ᵗʰ day to 7ᵗʰ day. Biochemical analysis was performed to detect the content of calcium (Ca) and phosphorus (P). Histological changes and degradation of extracellular matrix were observed through hematoxylin-eosin (H & E) and Masson staining. To further determine the mechanism, TUNEL staining and western blot were also performed to detect the apoptosis changes in growth plate of all groups. The results showed the disproportionation of Ca and P content and upregulation of apoptosis during the development of TD. But, after the administration of CGA, the ratio of Ca:P was upregulated, and the apoptosis was also downregulated. The current study shows the toxic effect of thiram on chickens and suggests that CGA is associated with a mechanism that plays a significant role in apoptosis induced by thiram in poultry industry.

The gill tissue is the main site of metabolic enzymes or compensation, with the kidney tissue playing a supporting role. At the gill tissue, carbonic anhydrase enzymes (CAs) catalyze the hydration of CO₂ to HCO₃⁻ and H⁺ for production to the H₂O. In this work, the CA enzyme was purified from horse mackerel (Trachurus trachurus) gill with a specific activity of 21,381.42 EU/mg, purification fold of 150.61, total activity of 2347.68 EU/mL, and a yield of 16.13% using sepharose 4B-L-tyrosine-sulfanilamide affinity gel chromatography. For recording the enzyme purity, gel electrophoresis was performed, and single band was seen. The molecular weight of this enzyme was found approximately 35 kDa. Also, the inhibitory effects of different pesticides such as thiram, clofentezine, propineb, deltamethrin, azoxystrobin, and thiophanate and heavy metal ions such as Fe²⁺, Cu²⁺, Co²⁺, Pb²⁺ Hg²⁺, and As³⁺ on horse mackerel gill tissue CA enzyme activities were investigated. Our results indicated that these pesticides and metal ions showed inhibitory effects at low nanomolar and millimolar concentrations for fish gill CA enzymes, respectively.

Investigations of deleterious effects on non-target species, including earthworms, have been conducted for a number of pesticides, but there is a need for additional assessments of potential adverse effects. In the present study, the acute toxicity of eight pesticides to the earthworm Eisenia andrei was assessed and compared. The exposures were conducted using the filter paper contact toxicity method. Based on the 48-h LC₅₀ values, one pesticide was classified as supertoxic (combined fungicide containing difenoconazole and fludioxonil), four as extremely toxic (combined herbicide containing pethoxamide and terbuthylazine, combined fungicide containing fluopyram and tebuconazole, fungicide containing pyrimethanil, and combined fungicide containing thiram and carboxin), two as very toxic (combined fungicide containing flutriafol and thiabendazole, and herbicide containing fluroxypyr-meptyl), and one as moderately toxic (insecticide containing thiamethoxam). Additionally, effects of pesticides on the multixenobiotic resistance (MXR) activity were measured. Results showed that four pesticides caused significant effects with a recorded inhibition of the activity, which can consequently lead to a higher toxicity due to longer retention of the pesticides in the cells. Finally, for three chosen pesticides, gene expression of cat, sod, and gst was measured, and significant changes were observed. The obtained results show that earthworms could be significantly affected by pesticides commonly used in agriculture.

The widespread use of thiram has raised concerns for health and its toxic effects, but the underlying toxicity mechanism on platelets and bones is poorly defined. Here, we found a significant increase in the number of platelets in chickens with the thiram intake, due to the increased expression of thrombopoietin mRNA in the dysfunction liver. Furthermore, the decreased vascular distribution and cell death of chondrocytes in the tibial growth plates (TGPs) were observed, resulting in bone growth inhibition, which is associated with the abnormal activation of platelets leading to the extraordinary decrease of vascular endothelial growth factor A (VEGFA) and angiopoietin-1 protein were released and their corresponding receptors VEGFR2 and Tie-2 expressions were also reduced in the TGPs. Taken together, these findings revealed that thiram has an adverse effect on bones and platelets, which may have a high risk of thrombosis and osteoarthritis.

A semiconductor photocatalyst was prepared in facile, standard conditions by integrating 1% metal-free, sulphur-doped graphene oxide (sGO) as cocatalyst and Ag₃VO₄ as photocatalyst and characterised via spectroscopic, microscopic and voltammetric techniques. The catalytic activity was performed on notable water pollutants like textile dyes and fungicide employing various techniques. Cationic dyes such as methylene blue and rhodamine B were degraded > 99% with above 90% organic carbon content removal indicating total mineralisation while anionic dyes were degraded 75–80% in 1 h. For the first time, a dithiocarbamate fungicide thiram is degraded to give thiourea as a product in 1 h. Photocatalysis follows pseudo-first order kinetics with rate 3.67, 49.50 and 3.19 times higher than Ag₃VO₄, sGO and GO-Ag₃VO₄ respectively with excellent stability and recyclability. One percent sGO aided excellent carrier separation boosted by electrons and surface defects from sGO, morphology and n-n heterojunction formation. The catalyst efficiently removed 82.8% of the total organic carbon content of a real water sample from the textile mill under 2-h sunlight irradiation.

Carbendazim and thiram are fungicides used in combination to prevent mold destruction of crops. Studies have demonstrated genotoxicity by these agents, but have not used concentrations below their water solubility limits in drinking water to test for persistence of genotoxicity due to chronic exposure. Ten 8-week old male Swiss-Webster mice were exposed to tap water, or nominal concentrations of 20 μM carbendazim, 20 μM thiram or 20 μM of both fungicides for 90 days (total of 40 mice). Five mice from tap water controls, carbendazim, thiram and combination-treated groups (20 mice total) had genotoxicity detected by comet assay of lymphocytes at the termination of the exposure period. The other 20 mice (4 treatment groups) were all switched to tap water and allowed a 45-day recovery period to check for persistence of DNA damage. The damage was compared with commercial control cells exposed to increasingly harsh treatment by etopside. Comet assay (mean % tail DNA + SE) of control mice (9.8 + 0.9) was similar to commercial control (CC0) cells (8.5 + 0.9). Carbendazim, thiram or the combination treatment caused similar mean % tail DNA with 33.0 + 2.9, 30.1 + 3.3 and 29.1 + 1.8, respectively, comparable with commercial cells slightly damaged by etopside (CC1 with 31.4 + 2.9) with no statistical change in water or food intake, body weight or liver or kidney weights. The key result was that a 45-day recovery period had no observable difference in the DNA damage as assessed by DNA % in comet tail with tap water controls and CCO control cells at 7.0 + 0.7 and 9.7 + 1.2 versus 27.5 + 1.9, 29.3 + 2.2 and 32.0 + 1.8, respectively, for carbendazim, thiram and combination treatments. It is of concern that the use of these agents in developing countries with little training or regulation results in water pollution that may cause significant persistent DNA damage in animal or human populations that may not be subject to repair.

Tetramethyl thiuram disulfide (thiram) is one of the important pesticides, which is extensively used in agriculture, but if it is combined with the cell membrane, then it causes membrane damage, bone morphogenic inactivation, and inhibited angiogenesis. Thiram has been considered a common cause of tibial dyschondrolplasia (TD) in various avian species, because it becomes the part of feed due to environmental contamination and its overuse in agriculture as pesticides or fungicide. However, there is no systematic study on the changes of the correlation indexes with toxic effect of the thiram in chickens. Therefore, we evaluated the toxic effects of thiram on growth performance of chickens, viscera organ index, pathological changes in tissue, and gene expression associated with osteoblast differentiation, vascularization, and tibial bone development. For this study, 1-day chickens (n = 300) were randomly distributed into two equal groups, control group (normal basal diet) and thiram group (adding thiram 40 mg/kg in basal diet). The result presented that thiram group chickens were looking unhealthy, lazy, and showing clinical symptoms like lameness. Thiram treatment significantly reduced the performance of chickens, liver index, and tibial length compared with control group. The toxic effect of thiram increased the visceral organ index (spleen and cardiac), tibia index, and TD severity considerably. It also increased serum Ca²⁺ and P³⁺ concentration and decreased tibial density compared to control chickens but the difference was not significant. Histopathology of tibia and liver showed that there were severe lesions due to toxic effect of thiram. Furthermore, HIF-1α and VEGF antibody localizations were increased and WNT4 localization was reduced significantly in immunohistochemical analysis. This systemic study of toxic effects of thiram in chicken concluded that thiram reduced the growth performance of chickens through decreasing liver index, whereas increasing kidney, cardiac, and spleen index, and induced TD by changing the expressions of VEGF, HIF-1α, and WNT4.