Chlorinated polyfluoroalkyl ether sulfonates (Cl-PFESAs), are ubiquitous alternatives to perfluorooctane sulfonate (PFOS), a widely used poly- and perfluoroalkyl substance (PFAS). Despite in vivo and in vitro evidence of metabolic toxicity, no study has explored associations of Cl-PFESAs concentrations with metabolic syndrome (MetS) in a human population. To help address this data gap, we quantified 32 PFAS, including 2 PFOS alternative Cl-PFESAs (6:2 and 8:2 Cl-PFESAs) in serum from 1228 adults participating in the cross-sectional Isomers of C8 Health Project in China study. The odds ratios (ORs) and 95% confidence intervals (CIs) of MetS and its various components were estimated using individual PFAS as a continuous or categorical predictor in multivariate regression models. The association between the overall mixture of PFAS and MetS was examined using probit Bayesian Kernel Machine Regression (BKMR-P). Greater serum PFAS concentrations were associated with higher odds of MetS and demonstrated a statistically significant dose-response trend (P for trend < 0.001). For example, each ln-unit (ng/mL) increase in serum 6:2 Cl-PFESA was associated with a higher prevalence of MetS (OR = 1.52, 95% CI: 1.25, 1.85). MetS was also 2.26 (95% CI: 1.59, 3.23) times more common in the highest quartile of serum 6:2 Cl-PFESA concentration than the lowest, and particularly high among women (OR = 6.41, 95% CI: 3.65, 11.24). The BKMR-P analysis showed a positive association between the overall mixture of measured PFAS and the odds of MetS, but was only limited to women. While our results suggest that exposure to Cl-PFESAs was associated with MetS, additional longitudinal studies are needed to more definitively address the potential health concerns of these PFOS alternatives.

With the growing production and use of carbon nanomaterials (CNMs), the risk of their releases to the environment has drawn much attention. However, their potential effect on soil invertebrates has not yet been systematically assessed. Herein, the toxic effects of graphene oxide (GO) on earthworms (Eisenia fetida) were thoroughly investigated. Exposure to different doses of GO (0, 5, 10, 20, and 30 g kg⁻¹) was conducted for 7, 14, 21, and 28 days. The results showed that enzymatic activity was stimulated at the early stages of exposure (7 days and 14 days) and inhibited after 14 days for catalase (CAT) and after 21 days for peroxidase (POD) and superoxide dismutase (SOD), especially at high GO doses. The content of MDA showed an increasing trend over the whole exposure period and was significantly elevated by GO from 21 days except at the dose of 5 g kg⁻¹on day 21. Lysosomal membrane stability and DNA damage presented dose- and time-dependent relationships. Graphene oxide remarkably decreased lysosomal membrane stability except at the dose of 5 g kg⁻¹ on day 7. The tail DNA%, tail length and olive tail moment increased with increasing GO dose throughout the exposure duration, reaching maximum values at the end of exposure (28 days). These findings suggest that GO induces oxidative stress and genotoxicity in Eisenia fetida, resulting in lipid peroxidation, decreased lysosomal membrane stability and DNA damage. Therefore, attention should be paid to the potential pollution and risk associated with graphene oxide application. The results can provide valuable information for environmental safety assessment of graphene nanomaterials in soil.

HONO is an important component of reactive nitrogen (Nᵣ) and precursors of OH radical. However, the source and removal of HONO are not clear. Here, measurements of HONO (May 18–31, 2018) were conducted in Xi'an and Xianyang simultaneously for the first time. The relationship between HONO and other Nᵣ (such as NO and NO₂) in two cities was analyzed. The mixing ratio of HONO in Xi'an was 1.2 ± 0.8 ppbv, and that in Xianyang was 1.2 ± 1.1 ppbv. The nighttime HONO mixing ratio was higher in Xianyang, while the daytime HONO was higher in Xi'an. Compared with the contribution from heterogeneous process of NO₂, direct emissions and homogeneous processes (NO + OH) were less important for nocturnal HONO formation in these two cities. The relative contribution of heterogeneous process in Xianyang was more important than that in Xi'an. The reaction of NO₂ upon aerosols surface was identified as an important source of HONO for two sites. The conversion of NO₂ on the other surfaces might attend the heterogeneous formation of HONO in Xianyang site. Daytime HONO budget analysis indicated that there was an additional unknown formation process of HONO at two sites. The net OH production rate from HONO (from 08:00 to 17:00) was 1.6 × 10⁷ and 1.3 × 10⁷ molecule/(cm³ s) for Xian and Xianyang, 5.2 and 3.5 times higher than from O₃ photolysis. Besides, a dust storm appeared during this observation period, and the impact of local emission and transport processes was separately analyzed. The sources, characteristics, and effects of HONO identified in this study laid a foundation for further research on HONO and air pollution in the Guanzhong area.

Discharged carbon nanotubes (CNTs) likely interact with co-existing organic contaminants (OCs) and pose joint toxicity to environmental microbes. Herein, hydrophobic pentachlorophenol (PCP) and hydrophilic ciprofloxacin (CIP) were used as representative OCs and their joint toxicities with CNTs to Bacillus subtilis were systematically investigated at cellular, biochemical, and omics levels. The 3-h bacterial growth half inhibitory concentrations of CNTs, PCP, and CIP were 12.5 ± 2.6, 3.5 ± 0.5, and 0.46 ± 0.03 mg/L, respectively, and they all could damage cell membrane, increase intracellular oxidative stress, and alter bacterial metabolomics and transcriptomics; while CNTs-PCP and CNTs-CIP binary exposures exhibited distinct additive and synergistic toxicities, respectively. CNTs increased bacterial bioaccumulation of PCP and CIP via destabilizing and damaging cell membrane. PCP reduced the bioaccumulation of CNTs, while CIP had no significant effect; this difference could be owing to the different effects of the two OCs on cell-surface hydrophobicity and CNTs electronegativity. The additive toxicity outcome upon CNTs-PCP co-exposure could be a result of the balance between the increased toxicity from increased PCP bioaccumulation and the decreased toxicity from decreased CNTs bioaccumulation. The increased bioaccumulation of CIP contributed to the synergistic toxicity upon CNTs-CIP co-exposure, as confirmed by the increased inhibition of topoisomerase Ⅳ activity and interference in gene expressions regulating ABC transporters and lysine biosynthesis. The findings provide novel insights into environmental risks of CNTs.

The European Noise Directive proposes using strategic noise maps as tools to assess populations affected by environmental noise. It recommends using computational methods instead of in situ measurements when possible. A sound source’s emission power is an important factor in the calculation of noise indicators. For traffic noise, this parameter is usually defined based on vehicle flow considering an emission spectrum that depends on the type of vehicle and its speed. This study analysed the possibility of using the categorisation method to propose an alternative method of defining a sound source’s emission power to develop noise maps. This was accomplished using previously published values of the emission power per unit length. Another method is also proposed that estimates traffic flows. To verify their estimation capacity, the results of both methods were compared with the values obtained from in situ measurements. The results demonstrated similar uncertainties in both methods and were in the range of expected average uncertainties compared to the results obtained by calculating a noise map with the measured experimental values. In particular, for the differences between calculations and measurements, in absolute values, the mean uncertainties were approximately 2 dBA in estimating different long-term noise indicators. For the differences, the mean of the uncertainties obtained via the categorisation method did not present significant differences for the null value for all the analysed noise indicators. Street stratification is a rapid and low-cost approach for road traffic noise mapping without increasing uncertainties.

Phosphogypsum (PG) is disposed worldwide in large stacks usually placed in coastal zones, as in the case of Huelva (SW of Spain), where around 100 Mt of PG are stored on the salt marshes of the Tinto River estuary covering a surface of about 1000 ha. This management generates the weathering of PG, and due to its high acidity (pH ≈ 2) and pollutant load can provoke significant emissions into their surroundings. In this work were evaluated by laboratory experiments the effects of pH increase in the behaviour of heavy metals and natural radionuclides during the mixing of phosphogypsum leachates with seawater.The acidic phosphogypsum leachates showed concentrations of heavy metals from two to three orders of magnitude higher than natural continental waters, and natural radionuclides (U-isotopes and ²¹⁰Po) from four to five orders of magnitude higher than unperturbed aquatic systems. Major elements and some heavy metals as Mn, Ni, Cd, As, Sb and Co showed a conservative behaviour during the neutralisation of the leachates with seawater, remaining in the liquid phase, while other ones as Al, Fe, Cr, Zn, Cu, Pb precipitated and/or were adsorbed onto the solid phase. The U-isotopes and ²¹⁰Po showed a clear non-conservative behaviour probably due to coprecipitation/adsorption processes onto the formed precipitates, but while ²¹⁰Po reached a total removal at pH ≈ 7, U- isotopes after a total removal at pH ≈ 5 returned into the liquid phase due to redissolution/desorption processes at near neutral pH.The formed precipitates, mainly composed by iron phosphates particles, showed heavy metal and natural radionuclide concentrations from one to three orders of magnitude higher than unperturbed soils. All these facts demonstrate the serious environmental impact produced by the PG stacks into their surroundings and the urgency of effective restoration measures.

Di (2-ethylhexyl) phthalate (DEHP) is a widespread plasticizer that persists in the environment and can significantly contribute to serious health hazards of liver especially oxidative stress injury. Lycopene (LYC) as a carotenoid has recently gained widespread attention because of antioxidant activity. However, the potential mechanism of DEHP-induced hepatotoxicity and antagonism effect of LYC on it are still unclear. To explore the underlying mechanisms of this hypothesis, the mice were given by gavage with LYC (5 mg/kg) and DEHP (500 or 1000 mg/kg). The data suggested that DEHP caused liver enlargement, reduction of antioxidant activity markers, increase of oxidative stress indicators and disorder of cytochrome P450 enzymes system (CYP450s) homeostasis. DEHP-induced reactive oxygen species (ROS) activated the NF-E2-relatedfactor2 (Nrf2) and nuclear xenobiotic receptors (NXRs) system including Aryl hydrocarbon receptor (AHR), Pregnane X receptor (PXR) and Constitutive androstane receptor (CAR). Interestingly, these disorders and injuries were prevented after LYC treatment. Taken together, DEHP administration resulted in hepatotoxicity including oxidative stress injury and disordered CYP450 system, but these alterations might be ameliorated by LYC via crosstalk between AHR–Nrf2 pathway.

Systemic toxicity, particularly, developmental defects of humidifier disinfectant chemicals that have caused lung injuries in Korean children, remains to be elucidated. This study evaluated the mechanisms of the adverse effects of 5-chloro-2-methyl-4-isothiazoline-3-one/2methyl-4-isothiazolin-3-one (CMIT/MIT), one of the main biocides of the Korean tragedy, and identify the most susceptible developmental stage when exposed in early life. To this end, the study was designed to analyze several endpoints (morphology, heart rate, behavior, global DNA methylation, gene expressions of DNA methyl-transferases (dnmts) and protein profiling) in exposed zebrafish (Danio rerio) embryos at various developmental stages. The results showed that CMIT/MIT exposure causes bent tail, pericardial edema, altered heart rates, global DNA hypermethylation and significant alterations in the locomotion behavior. Consistent with the morphological and physiological endpoints, proteomics profiling with bioinformatics analysis suggested that the suppression of cardiac muscle contractions and energy metabolism (oxidative phosphorylation) were possible pivotal underlying mechanisms of the CMIT/MIT mediated adverse effects. Briefly, multi-level endpoint analysis indicated the most susceptible window of exposure to be ≤ 6 hpf followed by ≤ 48 hpf for CMIT/MIT. These results could potentially be translated to a risk assessment of the developmental exposure effects to the humidifier disinfectants.

There is growing evidence of widespread contamination of freshwater ecosystems with microplastics. However, the effects of chronic microplastic ingestion and its interaction with other pollutants and stress factors on the life-history traits and the host-microbiome of aquatic invertebrates are not well understood. This study investigates the effects of exposure to sediment spiked with 1 μm polystyrene-based latex microplastic spheres, an environmentally realistic concentration of a pyrethroid pesticide (esfenvalerate), and a combination of both treatments on the life-history traits of the benthic-dwelling invertebrate, Chironomus riparius and its microbial community. The chironomid larvae were also exposed to two food conditions: abundant or limited food in the sediment, monitored for 28 and 34 days respectively. The microplastics and esfenvalerate had negative effects on adult emergence and survival, and these effects differed between the food level treatments. The microbiome diversity was negatively affected by the exposure to microplastics, while the relative abundances of the four top phyla were significantly affected only in the high food level treatment. Although the combined exposure to microplastics and esfenvalerate showed some negative effects on survival and emergence, there was little evidence for synergistic effects when compared to the single exposure. The food level affected all life-history traits and the microbiota, and lower food levels intensified the negative effects of the exposure to microplastics, esfenvalerate and their combination. We argue that these pollutants can affect crucial life-history traits such as successful metamorphosis and the host-microbiome. Therefore, it should be taken into consideration for toxicological assessment of pollutant acceptability. Our study highlights the importance of investigating possible additive and synergic activities between stressors to understand the effects of pollutants in the life story traits and host-microbiome.

Plastics are globally distributed in oceans and can pose a threat to the environment and organisms. In this study, plastic pollution in surface water and sediments of the Bohai Sea was assessed based on plastic abundance, distribution and characteristics (shape, polymer, size and color). Water and sediment samples were collected across the sea using a plankton net (330 μm) and a grab sampler, respectively. The following conclusions were reached. 1) In surface water, large plastics were less abundant (0.14 items/m³) and showed less diverse characteristics than microplastics (0.79 items/m³) but did not significantly differ in spatial distribution. 2) Microplastics in water were more abundant (1.95 items/m³) with more diverse characteristics in Liaodong Bay than in other regions of the sea (0.26–0.59 items/m³). Plastic waste from highly concentrated agricultural, industrial and fishery activities could make large contributions to microplastics in Liaodong Bay. Additionally, low hydrodynamics and long distance to Bohai Strait are unfavorable for diffusion of particles, facilitating the retention of microplastics and increasing the abundance in this bay. 3) Microplastics in sediments were smaller in terms of dominant sizes (<0.5 mm) with less diverse characteristics than particles in water (0.5–1.5 mm). Specifically, fragments, foams and lines dominated among the microplastics in water, whereas fibers and fragments were dominant particles in sediments; alkyd resin, polyethylene, polystyrene and polypropylene (PP) predominated among the particles in water, but rayon, cellulose and PP were dominant particles in sediments. 4) Neither abundance nor size of microplastics in the two media was proportionally correlated and showed low similarity indexes of polymer (0.16), shape (0.29) or color (0.38). This could be related to mismatch in spatiotemporal distributions and variations in the characteristics, fate and behavior of microplastics in the two media. The findings provide knowledge for tracing the sources of plastics in the Bohai Sea.