Binding of heavy metal ions by calcium alginate has been described in the literature with many different models. In the present study, two most basic models were used to systematically compare their simultaneous description of metal uptake dependence on pH and metal ion concentration in the bulk solution. The experimental datasets corresponding to the binary sorption systems containing protons and heavy metal ion (cadmium, lead, or copper) were taken from the literature. The applicability and limitations of both models are discussed. Neither of the models gave a completely satisfactory description of all data. The two-site occupancy model yielded better results compared to the one-site occupancy model when considering the coherence of the parameters (e.g., affinity constants) but the quality of the obtained fits is similar in both cases.

PURPOSE: The objective of this study was to determine the removal of zinc and copper by two freshwater green microalgae Chlorella pyrenoidosa and Scenedesmus obliquus and to investigate changes of algal ultrastructure and photosynthetic pigment. METHODS: Algal cells were exposed for 8 days to different initial zinc or copper concentrations. Heavy metal concentrations were detected by an atomic absorption spectrophotometer. Algal growth, ultrastructure, and photosynthetic pigment were analyzed by a microplate reader, transmission electron microscope, and spectrophotometer, respectively. RESULTS: Low zinc and copper concentrations induced increase in algal growth, whereas application of high zinc and copper concentrations suppressed the growth of both algae. High metal concentrations also decreased the photosynthetic pigments and destroyed algal cell ultrastructure. The zinc removal efficiency by both algae increased rapidly during the first day and thereafter remained nearly constant throughout the experiment. The copper removal efficiency by both algae increased slowly during the whole experimental periods. In all cultures, the quantity of both metals removed intracellularly was much lower than the adsorbed quantity on the cell surface. CONCLUSIONS: Both strains of the microalgae had proven effective in removing zinc and copper from aqueous solutions, with the highest removal efficiency being near 100%. In addition, C. pyrenoidosa appeared to be more efficient than S. obliquus for removing copper ions. On the contrary, S. obliquus appeared to be more efficient than C. pyrenoidosa for removing zinc ions.

PURPOSE: The present research aims to investigate the individual and interactive effects of chlorine dose/dissolved organic carbon ratio, pH, temperature, bromide concentration, and reaction time on trihalomethanes (THMs) formation in surface water (a drinking water source) during disinfection by chlorination in a prototype laboratory-scale simulation and to develop a model for the prediction and optimization of THMs levels in chlorinated water for their effective control. METHODS: A five-factor Box–Behnken experimental design combined with response surface and optimization modeling was used for predicting the THMs levels in chlorinated water. The adequacy of the selected model and statistical significance of the regression coefficients, independent variables, and their interactions were tested by the analysis of variance and t test statistics. RESULTS: The THMs levels predicted by the model were very close to the experimental values (R 2 = 0.95). Optimization modeling predicted maximum (192 μg/l) TMHs formation (highest risk) level in water during chlorination was very close to the experimental value (186.8 ± 1.72 μg/l) determined in laboratory experiments. The pH of water followed by reaction time and temperature were the most significant factors that affect the THMs formation during chlorination. CONCLUSION: The developed model can be used to determine the optimum characteristics of raw water and chlorination conditions for maintaining the THMs levels within the safe limit.

An assessment was made to monitor the short-term impact of heavily polluted sediments that may move out from the brackish man-made Lake Shihwa outside of the sea dike due to operations of a tidal power plant. Here, we exposed the Manila clam Ruditapes philippinarum collected from the western coast of Korea to natural sediment under lab condition for 96 h. Sediments were collected from Lake Shihwa and outside of the sea dike representing polluted and reference conditions, respectively. The results of chemical analysis revealed that the concentrations of nonylphenol and heavy metals in water and sediment from the inner region of Lake Shihwa were significantly higher than those of reference sediments. After 48 and 96 h of exposure, 30 specimens of clams were sampled from each experimental condition, and concentrations of nonylphenol and metals were measured in clams, water, and sediments. Several biomarkers, including concentrations of metallothionein-like proteins, and activities of the antioxidant enzymes glutathione S-transferase and catalase were determined in clams to characterize the effects of polluted sediments to clams. After 96 h of exposure, R. philippinarum assimilated nonylphenol up to 71 times compared to initial concentrations. However, there was no apparent uptake of heavy metals into the clams. Additionally, antioxidant enzymes exhibited higher activities in clams exposed to the polluted sediment. The results of the present study with physiological responses in R. philippinarum suggest that sediment transportation caused by the operation of a tidal power plant in Lake Shihwa will have striking effects on benthic organisms in the adjacent coastal area.

PURPOSE: The aim of this work was to assess the levels of copper and zinc in fish from the main freshwater ecosystems of Moldova, in relation with species, habitat, age, sex, season, and development stage. METHODS: Fish from Cyprinidae and Percidae families (Cyprinus carpio, Carassius auratus gibelio, Rutilus rutilus heckeli, Abramis brama, Aristichthys nobilis, Hypophtalmichthys molitrix, Sander lucioperca) were collected from Prut and Dniester rivers, Cuciurgan, Dubasari, and Costesti-Stanca reservoirs, and ponds of farms in the Dniester delta. The Cu and Zn content of fish tissues (skeletal muscles, liver, gonads, gills, skin, and scales) was determined by flame atomic absorption spectrophotometer AAS-3, of water by graphite furnace HGA 900 of AAnalist 400. RESULTS: The level of heavy metals accumulation in muscles of immature fish follows their dynamics in water. The highest concentration of zinc was registered in the gonads of mature fish, and of copper—in the liver. The lowest Cu and Zn contents were recorded in the muscles and are in the United Nations Food and Agriculture Organization safety-permissible levels for human consumption. Cu and Zn contents in muscles of fish depend on specimen age. Their level in fish gonads was sharply increasing during pre-spawning period. During the early developmental stages, the metal concentration in fish eggs and larvae varies within wide limits, but the accumulation pattern is similar in the investigated species. CONCLUSIONS: The fish represent one of the most indicative factors for the estimation of trace metals pollution in freshwater systems and this is important not only for monitoring purposes, but also for the fish culture ones.

INTRODUCTION: Concentrations and congener profiles of polychlorinated biphenyls (PCBs) and their hydroxylated metabolites (OH-PCBs) in placenta samples from a Madrid population (Spain) are reported. Structure dependent retentions of OH-PCBs are known to occur in both humans and wildlife, making it of interest to assess placental transfer of both parent compounds and their metabolites to the developing foetus. RESULTS: The ΣPCB concentrations found in placenta samples were in the range 943–4,331 pg/g fresh weight (f.w.), and their hydroxylated metabolites showed a 20-time lower concentration level (53–261 pg/g f.w.). The PCB profiles were surprisingly dominated by CB-52 and CB-101 accounting for more than 44% of the total PCB concentration. This is indicating a source of exposure that is not yet identified. The OH-PCB profiles were dominated by 4-OH-CB187 and 4-OH-CB146, representing >50% of the ΣOH-PCB concentration of the placenta samples. Statistical analysis of the data revealed strong correlations between the PCB congeners, among some OH-PCBs, and between OH-PCB metabolites with a meta- and para- substitution pattern. Both PCB and OH-PCB concentrations presented homogeneous distribution, what allowed the establishment of a partial least squares model that correlated the concentrations of OH-PCB with those of PCBs in placenta samples. In addition, causal correlations were observed between the concentrations of OH-PCBs and those of their corresponding PCB precursors.

INTRODUCTION: With quick responses to environmental changes, easy sampling, relative immobility, increasing availability of easily used taxonomic references, and allowing standardization for temporal and spatial comparisons, the biodiversity measures of microperiphyton communities have widely been accepted as useful indicators to evaluate environmental stress and anthropogenic impact. MATERIALS AND METHODS: The influence of sampling sufficiency for biodiversity analysis of microperiphyton communities was studied using a range of statistical methods in coastal waters of the Yellow Sea, northern China, from May to June 2010. Samples were collected from two depths using an artificial substrate. RESULTS: Sampling sizes represented a significant influence on biodiversity analysis of microperiphyton communities, e.g., 20 slide replicates (350 cm2) were sufficient for the microperiphyton communities at both depths, while 10 slide replicates (175 cm2) could meet the sampling strategy only for the samples with colonization times of 10 days or more at a depth of 1 m for recovering 90% species during the study period. Otherwise, more slide replicates were required with the increase of water depths and shortening colonization times for recovering microperiphyton species, e.g., for recovering 90% species of a “mature” microperiphyton community (>10 days), ∼10 slide replicates (∼175 cm2) were sufficient at a depth of 1 m, while for the “young” samples (>10 days) much more (15–30) slide replicates were required at both depths in this study. Furthermore, to achieve <10% standard errors, six (105 cm2) and nine (∼160 cm2) slide replicates were required for biodiversity analysis of the microperiphyton communities with various colonization times at depths of 1 and 3 m during the summer season, respectively. CONCLUSION: These results suggest that sampling sizes represented a significant influence on biodiversity analysis of microperiphyton communities for monitoring programs and ecological conservation researches in marine ecosystems.

PURPOSE: We measured offspring sex ratios in a resident insectivorous passerine bird, the Great tit Parus major, to find out whether metal pollution around a Finnish copper smelter would bias sex ratios in this territorial forest bird species. Besides a direct effect of pollutants, we were interested in possible indirect effects of pollution-related resource limitation, i.e., changes in food quality and quantity, on sex ratio. METHODS: We manipulated the diet quantity and quality (carotenoid levels) of P. major nestlings in polluted and unpolluted environments to find out if variation in dietary resource levels has any effect on fledgling sex ratios. Faecal metal levels of nestlings were analysed to monitor the level of pollution exposure at each territory. Near the time of fledging (at the age of 15 days), feather samples were collected for molecular sex determination. RESULTS: We did not find any association between metal exposure levels and offspring sex ratio, and thus, our results do not support the hypothesis that metal pollution would have direct effects on P. major offspring sex ratio. We found that, irrespective of pollution level, high nestling mortality was associated with male-biased sex ratio, most likely due to sex-specific mortality. Our feeding treatment, however, showed no significant effects on offspring sex ratio, possibly because natural variation in food availability outweighed the effect of our food manipulation on nestling mortality. CONCLUSIONS: We conclude that metal exposure at the levels found in our study area does not bias fledgling sex ratios in this species, but biased sex ratios may follow under natural or pollution-related resource limitation.

INTRODUCTION: The recognition that industrial activity plays an essential role in a sustainable society is now widespread. To understand the causal relationship between industrial pollution abatement expenditure and industrial system cleaning level in China is of considerable importance, especially under extremely rapid industrial growth and serious pressure of industrial pollutants abatement context. STUDY METHODS: We use composite index assessment method and regression analysis in this paper. We establish the mathematical model between composite industrial cleaner index and investment intensity for industrial pollutants abatement, and analyze the effects of industrial pollutants treatment and discharge indicators on composite industrial cleaner index in China. RESULTS AND DISCUSSION: Results show that: (1) There is significant nonlinear relationship between composite industrial cleaner index and investment intensity for industrial pollutants abatement. (2) From single indicator perspective, the effect of investment intensity on pollutants treatment indicators is positively, on the contrary, the effect of investment intensity on pollutants discharge indicators is negatively; (3) From decomposition cleaner index perspective, the effect of pollutants discharge level (process control) is higher than pollutants treatment capacity (end-of-pipe) on composite industrial cleaner index; (4) There is threshold between investment intensity and composite cleaner industrial index, it is a crucial reference scale for industrial environmental management in selected period.

PURPOSE: This study aims to examine whether addition of immobilising agents to a sandy, alkaline (pH = 8.1) soil, which had been contaminated with Pb and Zn by airborne particles from a Pb/Zn smelter, would substantially reduce metal bioavailability. METHODS: The effectiveness of five waste materials (blast furnace (BF) slag, alum water treatment (WT) sludge, red mud, sugar mill mud and green waste compost) as metal immobilising agents was evaluated by incubating them with a contaminated soil for a period of 12 months at rates of 5% and 10% (w/w), after which, Rhodes grass was grown in the soils in a greenhouse study. RESULTS: Additions of WT sludge, BF slag and red mud reduced CaCl2, CH3COOH, HCl and EDTA-extractable Zn but compost and mill mud had no appreciable immobilising effects. Additions of all amendments reduced levels of CaCl2, CH3COOH and HCl-extractable Pb although concentrations of EDTA-extractable Pb remained unchanged. A sequential extraction procedure showed that additions of mill mud and compost increased the percentage of total Pb and Zn present in the oxidisable fraction whilst additions of the other materials increased the percentage present in the residual fraction. Rhodes grass yields were promoted greatly by additions of red mud, compost and particularly mill mud, and yields were negatively correlated with tissue Pb concentrations and extractable Pb. CONCLUSIONS: Red mud was the most effective material for lowering extractable Pb and Zn levels simultaneously while mill mud and compost were notably effective for Pb. A field evaluation in the study area is justified.