Ascidians are good monitors for assessing water quality, since they filter large volumes of water; however, little is known about how xenobiotics, including metals, can affect ascidian hemocytes. Metals can be either toxic or beneficial to health, inducing many different responses. The response mechanism depends on the class of metals to which organisms are exposed: essential, nonessential, and borderline. To analyze the influence of metals from different classes on the protective mechanisms of an ascidian, we investigated the production of nitric oxide (NO) after exposure to various concentrations of Mg, Mn and Pb over different time periods. We also determined the amounts of each metal in the hemocytes. Our results indicated that especially Pb could stimulate NO production. Although Pb induced the highest NO production, cell viability was not severely altered in all Pb concentrations and time periods. Ascidians might serve as biomonitor for Pb, since their vanadocytes accumulate Pb.

Microplastic pollutants occur in marine environments globally, however estimates of seafloor concentrations are rare. Here we apply a novel method to quantify size-graded (0.038–4.0mm diam.) concentrations of plastics in marine sediments from 42 coastal and estuarine sites spanning pollution gradients across south-eastern Australia. Acid digestion/density separation revealed 9552 individual microplastics from 2.84l of sediment across all samples; equating to a regional average of 3.4 microplastics·ml−1 sediment. Microplastics occurred as filaments (84% of total) and particle forms (16% of total). Positive correlations between microplastic filaments and wave exposure, and microplastic particles with finer sediments, indicate hydrological/sediment-matrix properties are important for deposition/retention. Contrary to expectations, positive relationships were not evident between microplastics and other pollutants (heavy metals/sewage), nor were negative relationships with neighbouring reef biota detected. Rather, microplastics were ubiquitous across sampling sites. Positive associations with some faunal-elements (i.e. invertebrate species richness) nevertheless suggest high potential for microplastic ingestion.

Important to conserve plant species in coastal wetlands throughout their life cycle. All life stages in these habitats are exposed to varying tidal cycles. It is necessary to investigate all life stages as to how they respond to varying tidal regimes. We examine three wetlands containing populations of an endangered halophyte species, each subjected to different tidal regimes: (1). wetlands completely closed to tidal cycles; (2). wetlands directly exposed to tidal cycles (3). wetlands exposed to a partially closed tidal regime. Our results showed that the most threatened stage varied between wetlands subjected to these varying tidal regimes. We hypothesis that populations of this species have adapted to these different tidal regimes. Such information is useful in developing management options for coastal wetlands and modifying future barriers restricting tidal flushing.

Natural stable isotopes, such as carbon (C) and nitrogen (N), are modern tools to assess geochemical processes. C and N in organic matter can carry fingerprints of their hydrologic flows and sedimentary processes, including any anthropogenic modification on the natural system. This study focuses on the determination of aliphatic and polycyclic aromatic hydrocarbons and isotopic ratio in the sediment of Todos os Santos Bay (TSB). The isotopic results of the total organic matter indicate varied contribution marine and terrigenous. Typical rates of PAHs mainly indicate a pyrogenic source and mixture between pyrogenic and petrogenic sources. Typical ratios for the n-alkanes indicate the presence of petroleum hydrocarbons. The isotopic composition of n-alkanes suggests a mixture of sources, with the possible contribution of petrogenic.

The necessity of having a process in place for adequate risk assessment of shipwrecks that pose a threat to the marine environment is today internationally acknowledged. However, retrieving the desired data for such a risk assessment can prove challenging. One means of addressing this problem is to make use of experts' knowledge and experience. The purpose of this paper is therefore to present and analyse data for risk assessment of shipwrecks derived by expert elicitation. The main outcome is the experts' estimations of (i) the generic probability of an opening in a shipwreck due to the occurrence of a number of activities and (ii) estimations of the degree to which site-specific and wreck-specific indicators affect the probability of opening. Results show that the derived information is applicable in probabilistic shipwreck risk assessment and that the VRAKA framework now contains needed information for integrating generic and site-specific information using Bayesian updating.

Between 2006 and 2013, we salvaged and necropsied 362 seabird specimens from Tern Island, French Frigate Shoals, Northwestern Hawaiian Islands. Plastic ingestion occurred in 11 of the 16 species sampled (68.75%), representing four orders, seven families, and five foraging guilds: four plunge-divers, two albatrosses, two nocturnal-foraging petrels, two tuna-birds, and one frigatebird. Moreover, we documented the first instance of ingestion in a previously unstudied species: the Brown Booby. Plastic prevalence (percent occurrence) ranged from 0% to 100%, with no significant differences across foraging guilds. However, occurrence was significantly higher in chicks versus adult conspecifics in the Black-footed Albatross, one of the three species where multiple age classes were sampled. While seabirds ingested a variety of plastic (foam, line, sheets), fragments were the most common and numerous type. In albatrosses and storm-petrels, the plastic occurrence in the two stomach chambers (the proventriculus and the ventriculus) was not significantly different.

The presence of fecal bacteria in seawater is one of the most important bio-indicator parameters of fecal pollution. In this study, the Bay of İzmir (in the eastern Aegean Sea), which is a critical area because of its relationship with marine transportation and industrial and commercial activities, was evaluated for its microbiological and physicochemical parameters through a monitoring program. The data were obtained from seasonally assembled surface seawater samples from 2015 to 2017 at 23 sampling stations. Bacteriological investigations were performed by membrane filtration technique. During the monitoring period, for stations at the inner and middle-outer part, it was found that the inner part is exposed to more number of fecal coliforms (8.8×102cfu/100mL) and fecal streptococci (1.1×103cfu/100mL). The monitoring analysis performed in this study showed that there was negative correlation between physicochemical parameters and the level of fecal bacteria, but no significance was recorded by the Pearson correlation test. Fecal contamination parameters should be routinely monitored for improving the environmental conditions of coastal cities.

The sediment rejection ability of 8 coral species of 5 families and 3 morphologies were assessed in a series of short term exposure tests over a sedimentation range of 0.5–40mgcm−2d−1 and one longer term exposure test of 235mgcm−2. Sediment accumulation rates on live corals and dead (enamel-covered) skeletons varied between morphologies, with branching species often more adept at self-cleaning. Flow rates (0–17cms−1) significantly affected sediment-shedding ability as did differences in particle sizes, with coarse silt rejected faster than fine silt, but only at very high (235mgcm−2) deposition rates. Siliciclastic sediment was rejected faster than carbonate sediments and smothering for many days by mms of low organic content carbonate sediment resulted in bleaching, but no mortality. The findings are discussed with respect to turbidity generated in natural and dredging-related resuspension events and in the context for impact prediction for dredging projects.

This study investigated the influence of water level and velocity on Escherichia coli levels over multiple tidal cycles in an urban microtidal estuary in Melbourne, Australia. Over 3,500 E. coli samples and high resolution water level and velocity measurements from two locations within the estuary were used for the analysis. E. coli negatively correlated with water level in the upper estuary which was proposed to be linked to increased resuspension of estuarine sediments during low tide. No relationship was found in the lower estuary, likely due to wet weather inputs dwarfing subtler tidal-related processes. Removal of wet weather data enabled significant relationships to emerge in the lower estuary: 1) positive with water level (when a 9-h shift applied corresponding to the phase shift between water levels and velocities) and; 2) positive with velocity (no shift applied). This supports a link between increased E. coli levels and tidal-related resuspension.

Hepatic gene expression and liver histology were examined in sand flathead (Platycephalus bassensis) from six locations in Port Phillip Bay, Victoria, Australia. Four sets of genes including thyroid-related genes (D1, D2, TTR, TRα and TRβ), metal metabolism-related genes (MT, MTF1, TF, Ferritin and FPN1), apoptosis-related genes (Diablo/SMAC1, Diablo/SMAC2 and CYP1A) and an endoplasmic reticulum stress biomarker gene (GRP78) were examined in female flathead using qRT-PCR. TRβ and Diablo/SMAC1 gene expression was significantly up-regulated in fish from all polluted sites compared to those from a reference site. The transcripts of TRα and FPN1 were significantly higher in flathead from Corio Bay, while the hepatic mRNA of TTR and GRP78 were significantly lower in those fish. Positive correlations were observed between Diablo/SMAC1 and CYP1A, D2 and TRβ, TRα and TRβ. This study demonstrates that application of pathway-based biomarker genes and histopathology can provide comprehensive information on the impact of environmental pollutants on fish.