Epidemiological studies and animal experiments have suggested that exposure to Di-(2-ethylhexyl) phthalate (DEHP) is strongly associated with an increase in blood pressure. However, the mechanisms that result in the detrimental effects of DEHP exposure on blood pressure are unclear. In our study, mice were orally exposed to DEHP dosages of 0.1, 1, 10 mg/kg/day for 6 weeks. The results showed that DEHP could induce a significant increase in systolic blood pressure (SBP) and heart rate, and a significant thickening of the ventricular wall. To explore the underlying mechanism, we measured the level of: angiotensin converting enzyme (ACE); bradykinin B2 receptor (BK2R); endothelial nitric oxide synthase (eNOS); bradykinin and Ca²⁺ in cardiac cytoplasm as well as in serum nitric oxide (NO). The results suggested that DEHP could induce an increase in ACE levels, and a decrease in bradykinin levels. Moreover, BK2R, Ca²⁺, eNOS and NO decreased when mice were exposed to 10 mg/kg/day DEHP. Interestingly, 5 mg/kg/day angiotensin converting enzyme inhibitor (ACEI) treatment inhibited the increase in blood pressure, and inhibited the decrease in the levels of BK2R, Ca²⁺, eNOS, and NO, that were induced by DEHP exposure. Our results suggest that DEHP might increase blood pressure by activating ACE expression, and inhibiting the bradykinin-NO pathway.

Epidemiological evidence suggests that phthalate plasticizers may act as “obesogens”, which are chemicals that exacerbate obesity. The gastrointestinal (GI) system is the primary exposure route for phthalates, however, the relationship between phthalate-driven perturbations of GI system functions that can influence obesity has yet to be examined. To address this knowledge gap, we exposed Danio rerio (zebrafish) for 60 days to either (1) Control feeding (5 mg/fish/day), (2) Overfeeding (20 mg/fish/day) or (3) Overfeeding with diethyl-hexyl phthalate (DEHP) (20 mg/fish/day with 3 mg/kg DEHP). After 60 days, Overfed and Overfed + DEHP zebrafish had elevated body mass, and hepatosomatic and gonadosomatic indices. RNAseq analysis of the GI revealed enrichment of gene networks related to lipid metabolism in the Overfed + DEHP group. Many of the enriched networks were under transcriptional control of peroxisome proliferator activated receptor alpha (pparα), a known modulator of lipid metabolism, immune function, and GI function. Real-time PCR confirmed that pparα was overexpressed in the Overfed + DEHP zebrafish, further revealing a pathway by which DEHP may influence lipid metabolism via the GI. These data increase our understanding of phthalate-driven effects on GI function and lipid metabolism, identifying gut-specific gene networks that may drive phthalate-exacerbated obesity.

The distribution pattern of root-associated bacteria in native plant growth in tailing dumps with extreme conditions remains poorly understood and largely unexplored. Herein we chose a native plant, Bidens bipinnata, growing on both an Sb tailing dump (WKA) and adjacent normal soils (WKC) to in-depth understand the distribution pattern of root-associated bacteria and their responses on environmental factors. We found that the rhizosphere microbial diversity indices in the tailing dump were significantly different from that in the adjacent soil, and that such variation was significantly related with soil nutrients (TC, TOC, TN) and metal(loid) concentrations (Sb and As). Some dominant genera were significant enriched in WKA, suggesting their adaption to harsh environments. Notably, these genera are proposed to be involved in nutrient and metal(liod) cycling, such as nitrogen fixing (Devosia, Cellvibrio, Lysobacter, and Cohnella), P solubilizing (Flavobacterium), and Sb and As oxidation (Paenibacillus, Bacillus, Pseudomonas, and Thiobacillus). Our results suggest that certain root-associated bacteria in tailing dump were governed by soil edaphic factors and play important ecological roles in nutrient amendments and metal cycling for the successful colonization of Bidens bipinnata in this tailing dump.

Recent studies have shown that neonicotinoids in pollen and honey (collected by honeybees) are likely to pose risks to honeybees. However, data on the integrated residue and spatial-temporal variation of neonicotinoids from noncrop plants, the principle sources of pollen for honey bees, are very limited, especially in China. In this study, we employed a novel assessment method based on the relative potency factor to calculate the integrated residue of seven neonicotinoids in pollen and honey samples collected from noncrop plants in 12 stations of Zhejiang province in three consecutive months. The integrated concentration of neonicotinoids (IMIRPF) ranged from no detected (ND) to 34.93 ng/g in pollen and ND to 8.51 ng/g in honey. Acetamiprid showed the highest detection frequency of 41.7%, followed by clothianidin (33.3%) and dinotefuran (22.2%). The highest IMIRPF occurred in April for stations in the fringe areas of Zhejiang province, whereas for stations in the central areas of Zhejiang province, the IMIRPF in May was relatively higher than the other two months. In terms of spatial change, the pollution variation of pollen samples in Lin'an—Tonglu—Pujiang was relative highly polluted—lightly polluted—highly polluted. For honey samples, spatial variation showed a single trend, and peak values were found in Wenzhou, which may be attributed to the local climate and farming practices. This fundamental information will be helpful to understand the effects of neonicotinoids on honeybees foraging habits.

Biochar addition to soil may change the hydrophobicity of amended soil and influence soil hydraulic properties. Soil hydrophobicity, i.e. soil water repellency (SWR) can interrupt water infiltration and form preferential flow leading to a potential risk of soil erosion or groundwater pollution. Up to date, the effect of different biochars on soil hydrophobicity remains unclear and the association of SWR with soil hydraulic properties is still unknown. To link the biochar hydrophobicity to SWR and soil water holding capacity (WHC), the surface structure and chemical composition of 27 biochars with different feedstocks and pyrolysis temperatures were characterized, and the SWR and soil WHC of biochar-added soil were investigated. Carboxylic groups on the biochar surface, surface area and pore volume were mostly influenced by pyrolysis temperature, which suggested the dominant factor determining the severity of biochar hydrophobicity was pyrolysis temperature. Hydrophilic soil became hydrophobic after biochar amendment. A higher addition rate led to a stronger SWR of hydrophilic soil. Biochar addition increased soil WHC of hydrophilic soil with low total organic carbon (TOC) content. Biochar did not have significant influence on SWR and soil WHC of hydrophobic soil with high TOC content. It implied that the influence of biochar on SWR and soil hydraulic properties mainly depended on soil original hydrophobicity and TOC content. Therefore, the properties of biochar and influence on soil hydrophobicity and hydraulic properties should be considered before processing biochar application.

Metabolic syndrome is an important risk factor for non-communicable diseases, particularly type 2 diabetes, coronary heart disease, and stroke. Long-term exposure to greenspace could be protective of metabolic syndrome, but evidence for such an association is lacking. Accordingly, we investigated the association between long-term exposure to greenspace and risk of metabolic syndrome.The present longitudinal study was based on data from four clinical examinations between 1997 and 2013 in 6076 participants of the Whitehall II study, UK (aged 45–69 years at baseline). Long-term exposure to greenspace was assessed by satellite-based indices of greenspace including Normalized Difference Vegetation Index (NDVI) and Vegetation Continuous Field (VCF) averaged across buffers of 500 and 1000 m surrounding the participants’ residential location at each follow-up. The ascertainment of metabolic syndrome was based on the World Health Organization (WHO) definition. Hazard ratios for metabolic syndrome were estimated using Cox proportional hazards regression models, controlling for age, sex, ethnicity, lifestyle factors, and socioeconomic status.Higher residential surrounding greenspace was associated with lower risk of metabolic syndrome. An interquartile range increase in NDVI and VCF in the 500 m buffer was associated with 13% (95% confidence interval (CI): 1%, 23%) and 14% (95% CI: 5%, 22%) lower risk of metabolic syndrome, respectively. Greater exposure to greenspace was also associated with each individual component of metabolic syndrome, including a lower risk of high levels of fasting glucose, large waist circumference, high triglyceride levels, low HDL cholesterol, and hypertension. The association between residential surrounding greenspace and metabolic syndrome may have been mediated by physical activity and exposure to air pollution.The findings of the present study suggest that middle-aged and older adults living in greener neighbourhoods are at lower risk of metabolic syndrome than those living in neighbourhoods with less greenspace.

An emission inventory of carbonyl sulfide (COS) from primary anthropogenic sources in China was compiled from 2010 to 2015. The national total emission was estimated at 174 Gg S yr⁻¹ in 2015, with an annually average sustainable growth rate of 7.2% from 2010 to 2015. Industrial sources, biomass burning, coal combustion, agricultural sources and vehicle exhaust contributed 68.2%, 22.0%, 6.1%, 2.2%, and 1.5%, respectively, of total COS emissions in 2015. Aluminum production, carbon black production, titania production, coke production, pulp and paper industry, were the main industrial sources of COS, with the emission about 118 Gg S yr⁻¹ COS in 2015, accounting for 98.5% of total direct emissions from the Chinese industry. The distribution of COS emissions in China showed significant differences at the provincial level. In Shandong and Xinjiang province, they were much bigger than other provinces, with total emissions of 31 and 15 Gg S yr⁻¹, respectively. Uncertainty analysis of COS emission inventories showed that the emission range was 70–267 Gg S yr⁻¹ at a 95% confidence interval. In this study, COS emissions in China were much higher than prior underestimated estimations, even for Asian and global emissions, which could be resolved part of missing sources in the global COS budget.

Few attempts have been made to systematically investigate the impacts of urbanization on PM₂.₅ concentrations in countries at different stages of economic development. In this study, a broad concept of urbanization that considers the transformations in the urban economy and the transport sector induced by urbanization is proposed to investigate the influence of urbanization on national PM₂.₅ concentrations for underdeveloped, developing and developed countries during 1998–2014. The results indicate that urbanization has a significant relationship with PM₂.₅ concentrations, but the magnitude of its influence varies among groups of countries with different development levels. First, the positive response of PM₂.₅ concentrations to increased urbanization and transport-related emissions in underdeveloped countries are noticeably stronger than that in developing and developed countries. Second, for developing countries, urbanization, transport-related emissions and industrialization all have a significant positive effect on national PM₂.₅ concentrations increase, although their impacts are unexpectedly smaller than those in the other groups of countries. Finally, increasing urbanization and the decrease in CO₂ emissions from manufacturing industry appear to reduce national average PM₂.₅ concentrations in developed countries, while the decrease in transport-related CO₂ emission is likely to cause the rise in national average PM₂.₅ concentrations.