A prototype package plant incorporating the rotating disc wastewater treatment process was tested on municipal wastewater at the Village of Pewaukee, Wisconsin, to evaluate its treatment capabilities and establish guidelines for operation and testing of a full-scale rotating disc demonstration plant soon to be put into operation at Pewaukee. The package plant included a rotating bucket feed mechanism, ninety-one 1.75 meter diameter discs divided into two stages, and a secondary clarifier with a sludge-removal mechanism. Variables tested included hydraulic loading, rotational disc speed, sludge recycle, and wastewater treatment as it varied with climatic conditions.

The Southern California coastal sewage outfalls were investigated in relation to their effect upon standing stocks of phytoplankton, and on primary production. Kinetic parameters for the assimilation of ammonium, nitrate, and urea were determined at the outfall sites using N-15 labeled substrates. Laboratory studies investigated the utilization of various forms of nitrogen by phytoplankton, mechanisms and rates of nitrogen assimilation, and enzymes of nitrogen assimilation. It was found that the maximum growth rate was a variable, while the saturation constant was uniform over a range of dilution rates of N-limited chemostat cultures. The chemical composition of phytoplankton varied with dilution rate in reproducible ways. By varying the dilution rate of such cultures one seems to regulate the degree of nitrogen-deficiency of the phytoplankton.

Effects of rainfall runoff from a beef cattle feedlot on the water quality of a small impoundment were determined. Changes in chemical concentration of impounded water and changes in the community structure of aquatic organisms were measured and related to the amount and composition of feedlot runoff received. Water quality changes were also monitored in a nearby reservoir which received no feedlot runoff to serve as a control. Changes in chemical concentration or population structure of organisms were not apparent for discharges of about one-part feedlot runoff to 40 parts receiving water. Runoff discharges for two pumping periods with each contributing one-fourth of the volume of the receiving water were shown to degrade water quality in the impoundment. Several significant chemical and biological changes occurred. The most dramatic reduction in the biological community was the suffocation of about 90% of the game fish in the impoundment.