Topsoils near active and abandoned mining and smelting sites are highly polluted by metal(loid) contaminants, which are often bound to particulates emitted from ore processing facilities and/or windblown from waste disposal sites. To quantitatively determine the contaminant partitioning in the soil particulates, we tested an automated mineralogy approach on the heavy mineral fraction extracted from the mining- and smelting-polluted topsoils exhibiting up to 1920 mg/kg As, 5840 mg/kg Cu, 4880 mg/kg Pb and 3310 mg/kg Zn. A new generation of automated scanning electron microscopy (autoSEM) was combined and optimized with conventional mineralogical techniques (XRD, SEM/EDS, EPMA). Parallel digestions and bulk chemical analyses were used as an independent control of the autoSEM-calculated concentrations of the key elements. This method provides faster data acquisition, the full integration of the quantitative EDS data and better detection limits for the elements of interest. We found that As was mainly bound to the apatite group minerals, slag glass and metal arsenates. Copper was predominantly hosted by the sulfides/sulfosalts and the Cu-bearing secondary carbonates. The deportment of Pb is relatively complex: slag glass, Fe and Mn (oxyhydr)oxides, metal arsenates/vanadates and cerussite were the most important carriers for Pb. Zinc is mainly bound to the slag glass, Fe (oxyhydr)oxides, smithsonite and sphalerite. Limitations exist for the less abundant contaminants, which cannot be fully quantified by autoSEM due to spectral overlaps with some major elements (e.g., Sb vs. Ca, Cd vs. K and Ca in the studied soils). AutoSEM was found to be a useful tool for the determination of the modal phase distribution and element partitioning in the metal(loid)-bearing soil particulates and will definitely find more applications in environmental soil sciences in the future.

Copper is known to interfere with fish olfaction. Although the chemosensory detection and olfactory toxicity of copper ions (Cu²⁺) has been heavily studied in fish, the olfactory-driven detection of copper nanoparticles (CuNPs)—a rapidly emerging contaminant to aquatic systems—remains largely unknown. This study aimed to investigate the olfactory response of rainbow trout to equitoxic concentrations of CuNPs or Cu²⁺ using electro-olfactography (EOG, a neurophysiological technique) and olfactory-mediated behavioural assay. In the first experiment, the concentration of contaminants known to impair olfaction by 20% over 24 h (EOG-based 24-h IC20s of 220 and 3.5 μg/L for CuNPs and Cu²⁺, respectively) were tested as olfactory stimuli using both neurophysiological and behavioural assays. In the second experiment, to determine whether the presence of CuNPs or Cu²⁺ can affect the ability of fish to perceive a social cue (taurocholic acid (TCA)), fish were acutely exposed to one form of Cu-contaminants (approximately 15 min). Following exposure, olfactory sensitivity was measured by EOG and olfactory-mediated behaviour within a choice maze was recorded in the presence of TCA. Results of neurophysiological and behavioural experiments demonstrate that rainbow trout can detect and avoid the IC20 of CuNPs. The IC20 of Cu²⁺ was below the olfactory detection threshold of rainbow trout, as such, fish did not avoid Cu²⁺. The high sensitivity of behavioural endpoints revealed a lack of aversion response to TCA in CuNP-exposed fish, despite this change not being present utilizing EOG. The reduced response to TCA during the brief exposure to CuNPs may be a result of either olfactory fatigue or blockage of olfactory sensory neurons (OSNs) by CuNPs. The observed behavioural interference caused by CuNP exposure may indicate that CuNPs have the ability to interfere with other behaviours potentially affecting fitness and survival. Our findings also revealed the differential response of OSNs to CuNPs and Cu²⁺.

Periphyton plays a significant role in heavy metal transfer in wetlands, but its contribution to cadmium (Cd) bioavailability in paddy fields remains largely unexplored. The main aim of this study was to investigate the effect of periphyton on Cd behavior in paddy fields. Periphyton significantly decreased Cd concentrations in paddy waters. Non-invasive micro-test technology analyses indicated that periphyton can absorb Cd from water with a maximum Cd²⁺ influx rate of 394 pmol cm⁻² s⁻¹ and periphyton intrusion significantly increased soil Cd concentrations. However, soil Cd bioavailability declined significantly due to soil pH increase and soil redox potential (Eh) decrease induced by periphyton. With periphyton, more Cd was adsorbed and immobilized on organic matter, carbonates, and iron and manganese oxides in soil. Consequently, Cd content in rice decreased significantly. These findings give insights into Cd biogeochemistry in paddy fields with periphyton, and may provide a novel strategy for reducing Cd accumulation in rice.

Windowsill, heavy metal-containing dust samples, collected at different building heights, may provide some insight into both source and human health risk. Windowsill dust samples were collected from the 1st to 9th floor (1.4–23.2 m above ground) near a lead smelter (1 km to the smelter) and in urban areas (4.2–7.3 km to the smelter) and separated into <10, 10–45 and 45–125 μm size fractions. Samples were extracted with artificial lysosomal fluid (ALF) and the physiologically based extraction test (PBET) (<10 μm fractions only), subjected to scanning electron microscopy-energy dispersive x-ray spectroscopy (SEM-EDS) and Pb isotopic analysis. Greater Pb concentrations were found in 10–45 μm fraction than the other size fractions; at the PX site, dust Pb concentrations increased with windowsill height, while an opposite trend was found at other sites. Isotopic analysis and SEM-EDS results supported this contention. Higher floor samples collected near the smelter were more affected by lead smelting than lower floor samples; lower floor samples collected at urban sites were more affected by resuspended Pb-laden particles from the ground than higher floors. The Pb bioaccessible fraction (BAF) in the ALF and PBET ranged between 68.9-90.1 and 1.3–17.0%, respectively; urban samples had greater BAF values than samples collected near the smelter. This, first of its kind investigation regarding Pb in dusts at different building heights, provides further insight for reducing human health risks within Pb smelter vicinities.

Fucus virsoides is an ecologically important canopy-forming brown algae endemic to the Adriatic Sea. Once widespread in marine coastal areas, this species underwent a rapid population decline and is now confined to small residual areas. Although the reasons behind this progressive disappearance are still a matter of debate, F. virsoides may suffer, like other macroalgae, from the potential toxic effects of glyphosate-based herbicides.Here, through a transcriptomic approach, we investigate the molecular basis of the high susceptibility of this species to glyphosate solution, previously observed at the morphological and eco-physiological levels. By simulating runoff event in a factorial experiment, we exposed F. virsoides to glyphosate (Roundup® 2.0), either alone or in association with nutrient enrichment, highlighting significant alterations of gene expression profiles that were already visible after three days of exposure. In particular, glyphosate exposure determined the near-complete expression shutdown of several genes involved in photosynthesis, protein synthesis and stress response molecular pathways. Curiously, these detrimental effects were partially mitigated by nutrient supplementation, which may explain the survival of relict population in confined areas with high nutrient inputs.

Growing evidence suggests that microplastics can adsorb antibiotics and may consequently exacerbate effects on the health of exposed organisms. Our current understanding of the combined effects of microplastics and antibiotics on antibiotic resistance genes (ARGs) in soil invertebrates is limited. This study aimed to investigate changes in the microbiome and ARGs in Enchytraeus crypticus following exposure to a soil environment that contained both microplastics and antibiotics. Tetracycline (TC), polyamide (PA) and polyvinyl chloride (PVC) were used to construct microcosms of polluted soil environments (TC, PA, PVC, PA+TC, PVC+TC). The differences in microbiomes and ARGs were determined by bacterial 16S rRNA gene amplicon sequencing and high throughput quantitative PCR. The results show that compared with the Control or microplastics alone treatments, TC was significantly accumulated in E. crypticus when exposed to TC alone or in combination with microplastics (P < 0.05), but there were no significant differences about TC accumulation between TC, PA+TC, and PVC+TC treated E. crypticus (P > 0.05). Microplastics and TC significantly disturbed the microbial community, and decreased the microbial alpha diversity of E. crypticus (P < 0.05). However, there were no significant differences between TC, microplastics and their combined exposure treatments, and no toxic synergies on the diversity of E. crypticus microbiome between tetracycline and microplastics in soil environment. All the treatments increased the diversity of ARGs in E. crypticus (39–49 ARGs vs. 25 ARGs of control). In particular, treatments combining PVC and TC or PA and TC exposure resulted in greater ARGs abundance than the treatments when E. crypticus was exposed to PVC, PA or TC alone. These results add to our understanding of the combined effects of microplastics and antibiotics on the ARGs and microbiome of soil invertebrates.

Phthalates are widely used as binders and plasticizers in industrial and consumer products but show diverse toxicity. We investigated the level of human exposure to phthalates in Beijing, one of the most densely populated cities in the world. In this study, 12 metabolites of phthalates were measured in 70 spot urine samples collected from Beijing residents from August 2017 to April 2018 using ultra high-performance liquid chromatography tandem mass spectrometry. We found that metabolites of phthalates were ubiquitous in all urine samples. Total concentrations of phthalate metabolites ranged from 39.6 to 1931 ng mL⁻¹, with median concentrations were in decreasing order of children (371 ng mL⁻¹)> younger adults (332 ng mL⁻¹)> older adults (276 ng mL⁻¹). Mono-n-butyl phthalate (MnBP) was the predominant compound, and occurred at concentrations greater than those reported for people in other countries. The mean values of estimated daily intakes (EDIs) of ∑phthalate were 35.2, 10.3 and 10.9 ng (kg-bm)⁻¹ d⁻¹ for children, younger adults and older adults, respectively. EDIs of di-n-butyl phthalate (DnBP), di-iso-butyl phthalate (DiBP) and di-(2-ethylhexyl) phthalate (DEHP) exceeded reference values suggested by the US Environmental Protection Agency and the European Food Safety Authority. When concentrations were normalized to volume or creatinine-adjusted, hazard quotients (HQs) for 40 of 70 participants exhibited larger HQs >1 for individual phthalates, which was indicative of potential for adverse effects. Thus, exposure to phthalates might be a critical factor contributing to adverse health effects in Beijing residents. To the best of our knowledge, this is the first study to establish a pre-baseline level of urinary phthalate metabolites among residents in Beijing.

Soil organic matter (SOM) affects arsenic (As) and antimony (Sb) mobility in soils under waterlogged conditions by acting as an electron donor, by catalyzing redox–cycling through electron shuttling and by acting as a competing ligand. This study was set up to disentangle these different effects of SOM towards As and Sb sorption in anaerobic soils. Nine samples were taken at different depths in an agricultural soil profile to collect samples with a natural SOM gradient (<1–40 g soil organic carbon kg⁻¹). The samples were incubated either or not under waterlogged conditions in an anaerobic chamber for 63–70 days, and glucose (5 g C kg⁻¹) was either or not added to the anaerobic incubated samples as an electron donor that neither acts as an electron shuttle nor as a competing ligand. The solid-liquid distribution coefficients (KD) of As and Sb were measured at trace levels. The KD values of As decreased ∼2 orders of magnitude upon waterlogging the SOM rich topsoil, while no additional changes were observed when glucose was added. In contrast, smaller changes in the As KD values were found in the low SOM containing subsoil samples, unless glucose was added that mobilised As. The Sb KD values increased upon reducing conditions up to factor 20, but again only in the high SOM topsoil samples. Surprisingly, the Sb immobilisation during waterlogging only occurred in Sb amended soils whereas the geogenic Sb was mobilised upon reducing conditions, although total dissolved Sb concentrations remained low (<10 nM). The change in As and Sb sorption upon waterlogging was similar in the SOM rich topsoil as in the low SOM subsoil amended with glucose. This suggests that the SOM dependent changes in As and Sb mobility in response to soil waterlogging are primarily determined by the role of SOM as electron donor.

The aim of this study was to examine the effects of arbuscular mycorrhizal (AM) fungi, biochar (BC) addition and phosphorus (P) fertilizer applications on the mycorrhizal response, biomass and elemental uptake of Trifolium repens in cadmium (Cd)-polluted soils. The results showed that mycorrhizal colonization were significantly decreased by 100 mg P kg⁻¹ fertilizer input. Moreover, AM fungi, BC addition and P fertilizer significantly increased shoot biomass accumulation at all treatments. In the absence of BC, the nitrogen (N), potassium (K), calcium (Ca) and magnesium (Mg) contents in the shoots were not affected by AM fungi after P fertilizer application, but the P content in the shoots significantly increased in response to AM fungi. In the absence of BC, both AM fungi and P fertilizer significantly reduced the Cd concentrations in the plant tissues as well as the soil diethylenetriaminepentaacetic acid (DTPA)-Cd concentration. These results indicated that the translocation factors (TFs) were influenced only by BC addition and that the roots could accumulate greater amounts of Cd than the shoots. On the basis of the hygienic standard for feed in China, the shoot Cd concentration in white clover was below the maximum permitted Cd concentration (1 μg g⁻¹) across all treatments. Therefore, it is suggested that no negative mycorrhizal-white clover symbiotic relationships were observed and T. repens could be a suitable forage species for planting in soils with low concentrations of Cd contamination when BC and P fertilizer are applied.