Understanding how essential and toxic elements are distributed in cereal grains is a key to improving the nutritional quality of cereal-based products. The main objective of this work was to characterize the distribution of Cd and of nutrients (notably Cu, Fe, Mn, P, S and Zn) in the durum wheat grain. Laser ablation inductively coupled mass spectrometry and synchrotron micro X-ray fluorescence were used for micro-scale mapping of Cd and nutrients. A dissection approach was used to quantitatively assess the distribution of Cd and nutrients among grain tissues. Micro X-ray absorption near-edge spectroscopy was used to identify the Cd chemical environment in the crease. Cadmium distribution was characterized by strong accumulation in the crease and by non-negligible dissemination in the endosperm. Inside the crease, Cd accumulated most in the pigment strand where it was mainly associated with sulfur ligands. High-resolution maps highlighted very specific accumulation areas of some nutrients in the germ, for instance Mo in the root cortex primordia and Cu in the scutellum. Cadmium loading into the grain appears to be highly restricted. In the grain, Cd co-localized with several nutrients, notably Mn and Zn, which challenges the idea of selectively removing Cd-enriched fractions by dedicated milling process.

The effect of long-term use on the catch efficiency of biodegradable gillnets was investigated during commercial fishing trials and in controlled lab aging tests. The relative catch efficiency between biodegradable and nylon gillnets was evaluated over three consecutive fishing seasons for Atlantic cod (Gadus morhua) in Norway. The biodegradable gillnets progressively lost catch efficiency over time, as they caught 18.4%, 40.2%, and 47.4% fewer fish than the nylon gillnets during the first, second, and third season, respectively. A 1000-hour aging test revealed that both materials began to degrade after just 200 h and that biodegradable gillnets degraded faster than the nylon gillnets. Infrared spectroscopy revealed that the chemical structure of the biodegradable polymer changed more than the nylon. Although less catch efficient than nylon gillnets, biodegradable gillnets have great potential for reducing both capture in lost fishing gear and plastic pollution at sea, which are major problems in fisheries worldwide. | publishedVersion

Repeated reports of microplastic pollution in the marine pinniped diet have emerged in the last years. However, only few studies address the drivers of microplastics presence and the potential implications for monitoring microplastic pollution in the ocean. This study monitored their in the scats (N = 205) of four pinniped species/subspecies at five different locations in the southern Pacific Ocean (Peru and Chile). Samples from all rookeries contained microplastics, and overall, 68% of the examined scats contained fragments/fibers, mostly blue colored. We confirmed that 81.5% of the fragments/fibers were anthropogenic in origin , but only 30% were polymers. Scats from Juan Fernandez Archipelago presented higher microplastic concentrations than continental rookeries. Also, the common diet in each location may influence the levels found in the samples. This study presents a useful non-invasive technique to track plastic pollution in top predator diets as bioindicators for future surveillance/management plans applied to different location. | Rufford Foundation: N 18815-1. Dirección de Investigación y Doctorados, Universidad Andres Bello. Comisión Nacional de Investigación Científica y Tecnológica (CONICYT), CONICYT FONDECYT: 11150548, 116504, 111609059. Instituto Antártico Chileno Grant: INACh RT_12_17. Conicyt PCI: REDI 170292, REDI 170403. Ministerio de Economia, Fomento y Turismo through Iniciativa Científica Milenio (Núcleo Milenio INVASAL). Direccion de Investigación Universidad de Variarais: DIUV 38/2013. Morris Animal Foundation fellowship: N D16ZO-413. Saint Louis Zoo. Chicago Zoological Society. Kansas City Zoo. Woodland Park Zoo.

peer reviewed | Due to migration of harbour porpoises towards more polluted areas like the North Sea and their sensitivity towards pollution, there is a need for proper conservation measures for this species. As a consequence, knowledge about the pollutant’s kinetics is required. The present study is the first to investigate the kinetics of PBDEs in marine mammals using PBPK modeling as a non-destructive tool for describing the chemical’s kinetics in a protected animal species. The models were developed and parameterized using data from the literature and Black Sea harbour porpoises through computer opti- mization. The predictability of these models in time was assessed by reverse dosimetry modeling using data from North Sea porpoises (1990e2008). From these predictions, PBDE 99 levels were found to decrease the fastest, followed by PBDE 153, 47 and 100. Results show that the PBPK models can be applied for harbour porpoises from different regions and also simulate time trends.

We simulate the combined natural and pollutant-induced survival of early life stages of NEA cod and haddock, and the impact on the adult populations in response to the time of a major oil spill in a single year. Our simulations reveal how dynamic ocean processes, controlling both oil transport and fate and the frequency of interactions of oil with drifting fish eggs and larvae, mediate the magnitude of population losses due to an oil spill. The largest impacts on fish early life stages occurred for spills initiated in Feb–Mar, concomitant with the initial rise in marine productivity and the earliest phase of the spawning season. The reproductive health of the adult fish populations was maintained in all scenarios. The study demonstrates the application of a simulation system that provides managers with information for the planning of development activities and for the protection of fisheries resources from potential impacts. | An annual profile of the impacts of simulated oil spills on the Northeast Arctic cod and haddock fisherie | publishedVersion

Chile is a leading agricultural producer and thus consumer of insecticides, herbicides, and fungicides. In Molina, Central Chile, a prospective cohort has been established for studying the incidence and risk factors of chronic diseases in the adult population. Our goals were to measure airborne current use pesticides (CUPs), assess their spatial distribution and potential sources, and estimate health risks for the population in Molina. CUPs were measured using passive air samplers (PAS), deployed on six sites from October 2015 to August 2016. Thirty-eight pesticides were analyzed using high performance liquid chromatography (HPLC), but only nine of them were detected. Chlorpyrifos (CPF) was detected with air concentrations ranging from 444 to 14 624 (pg m(-3)). Diazinon, atrazine, dimethoate, metolachlor, simazine, terbuthylazine and tebuconazole were also detected; only pendimethalin had concentrations as high as those of CPF, with a maximum of 14 927 (pg m(-3)). Backward wind trajectories were used to estimate locations of potential sources contributing to airborne CUPs concentrations. Most of the exposure to CUPs was associated with local sources, while regional sources southern/eastern/western of Molina appear to contribute as secondary sources (soil evaporation followed by atmospheric transport) in spring and summer seasons. A health risk assessment using US-EPA's methodology was carried out for inhalation exposure of detected pesticides. None of the measured CUPs were associated with a hazard quotient (HQ) greater than 1, indicating no significant risk due to inhalation of pesticides in Molina's population with the exception of the group of children below 12 years old. However, further investigations are needed to evaluate others CUPs exposure route such as food consumption and dermal exposure to improve our health risk estimations. | Comisión Nacional de Investigación Científica y Tecnológica (CONICYT) 15130011-15110020 180078 1161673 Vicerrectoria de Investigacion, Pontificia Universidad Catolica de Chile 9/2014 RECETOX Research Infrastructure LM2015051 CZ.02.1.01/0.0/0.0/16_013/0001761

peer reviewed | Very few ecotoxicological studies have been performed on long-term exposure under controlled conditions, hence limiting the assessment of the impact of chronic and diffuse chemical pressures on the health of aquatic organisms. In this study, an ecotoxicoproteomic approach was used to assess the integrated response and possible acclimation mechanisms in Gammarus fossarum following chronic exposures to Cd, Cu or Pb, at environmentally realistic concentrations (i.e. 0.25, 1.5 and 5 µg/L respectively). After 10-week exposure, changes in protein expression were investigated in caeca of control and exposed males. Gel-free proteomic analyses allowed for the identification of 35 proteins involved in various biological functions, for which 23 were significantly deregulated by metal exposures. The protein deregulation profiles were specific to each metal, providing evidence for metal-specific action sites and responses of gammarids. Among the tested metals, Cu was the most toxic in terms of mortality, probably linked with persistent oxidative stress. Moulting and osmoregulation were the major biological functions affected by Cu in the long-term. In Pb-exposed gammarids, significant deregulations of proteins involved in immune response and cytoskeleton were observed. Reproduction appears to be strongly affected in gammarids chronically exposed to Cd or Pb. Besides, modified expressions of several proteins involved in energy transfer and metabolism highlighted important energetic reshuffling to cope with chronic metal exposures. These results support the fact that metallic pressures induce a functional and energetic cost for individuals of G. fossarum with potential repercussions on population dynamics. Furthermore, this ecotoxicoproteomic study offers promising lines of enquiry in the development of new biomarkers that could make evidence of long-term impacts of metals on the health of organisms.

On March 11 2011, a tsunami caused by a magnitude 9.0 earthquake devastated the northeastern coast of Honshu, Japan.The present study was conducted to assess environmental health risks of the areas affected and socio-psychological status of the dislocated people in Ishinomaki. Samples of sludge, water, flies and rodents were collected in 20 urban neighborhood associations affected by the tsunami in July and August 2011, and in August 2012. A socio-psychological survey was conducted in two urban and one rural temporary housing complexes in 2012. Animal feed concentrates and fish from damaged factories were scattered along the coast which caused a strong odor and great number of flies. Removal of fish and feed along with spraying insecticides reduced the odor and the number of flies by August 2011. The sludge and water samples contained potentially hazardous bacteria, but none were highly pathogenic. Heavy metals in sludge were not in alarming quantities. A rodent was captured in one unit in August 2011, and monitoring in two units found that the log number of rodents captured increased significantly over time (slope=0.08, p = 0.005). In temporary housing complexes, those who originally lived in rural fishing villages wished to return to their homes more (64.2%, 9/14) than in urban areas (30.6%, 11/36, p = 0.06). Risk factors for depression included absence of friends (p = 0.011) or trusted person to counsel in the housing complexes (p = 0.003) and illness of the respondent or a family member (p = 0.003). In conclusion, overall environmental health risk was acceptable for living, and monitoring of rodents population was recommended. In addition, psychological and economical support was needed for evacuees in temporary housing complexes.