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Compliance Monitoring Devices (CMDs) are instruments indicating if the 10–50 μ m organism abundance in ballast water complies with the International Maritime Organizations' discharge standard. For that, they trade the presumed accuracy of detailed methods for speed and simplicity. In an experiment using UV-treated water, four CMDs were compared, using a Cohen's kappa analysis (ISO 3725), with two detailed methods: Fluorescence Microscopy (FM) and the Most Probable Number (MPN). Of two CMDs, BallastWISE and B-QUA ATP, the standard (non)-compliance limits were adapted. Correlated to FM (non)-compliance results, ranking resulted in: 1) BallastWISE, 2) MPN, 3) Ballast-Check-2 and 4) WALZ-PAM. The ATP method had too few data for this ranking. MPN proved problematic: the abundance of 10–50 μ m organism decreased while that <10 m organisms increased. It is concluded that in case of UV-treatment, the outcome of CMD-validations will depend on the detailed method, the CMD instrument, and the (non)-compliance limits chosen.

Residues detected in pollen collected by honey bees are often used to estimate pesticide exposure in ecotoxicological studies. However, for a more accurate assessment of pesticides effect on foraging pollinators, residues found directly on flowers are a more realistic exposure approximation. We conducted a multi-residue analysis of pesticides on pollen and nectar of melon flowers collected from five fields. The cumulative chronic oral exposure Risk Index (RI) was calculated for Apis mellifera, Bombus terrestris and Osmia bicornis to multiple pesticides. However, this index could underestimate the risk since sublethal or synergistic effects are not considered. Therefore, a mixture containing three of the most frequently detected pesticides in our study was tested for synergistic impact on B. terrestris micro-colonies through a chronic oral toxicity test. According to the result, pollen and nectar samples contained numerous pesticide residues, including nine insecticides, nine fungicides, and one herbicide. Eleven of those were not applied by farmers during the crop season, revealing that melon agroecosystems may be pesticide contaminated environments. The primary contributor to the chronic RI was imidacloprid and O. bircornis is at greatest risk for lethality resulting from chronic oral exposure at these sites. In the bumblebee micro-colony bioassay, dietary exposure to acetamiprid, chlorpyrifos and oxamyl at residue level concentration, showed no effects on worker mortality, drone production or drone size and no synergies were detected when pesticide mixtures were evaluated. In conclusion, our findings have significant implications for improving pesticide risk assessment schemes to guarantee pollinator conservation. In particular, bee pesticide risk assessment should not be limited to acute exposure effects to isolated active ingredients in honey bees. Instead, risk assessments should consider the long-term pesticide exposure effects in both pollen and nectar on a range of bees that reflect the diversity of natural ecosystems and the synergistic potential among pesticide formulations.

peer reviewed | Monitoring plastic ingestion by marine wildlife is important for both characterizing the extent of plastic pollution in the environment and understanding its effect on species and ecosystems. Current methods to detect plastic in the digestive system of animals are slow and invasive, such that the number of animals that can be screened is limited. In this article, magnetic resonance imaging (MRI) is investigated as a possible technology to perform rapid, non-invasive detection of plastic ingestion. Standard MRI methods were able to directly measure one type of plastic in a fulmar stomach and another type was able to be indirectly detected. In addition to MRI, other standard nuclear magnetic resonance (NMR) measurements were made. Different types of plastic were tested, and distinctive NMR signal characteristics were found in common for each type, allowing them to be distinguished from one another. The NMR results indicate specialized MRI sequences could be used to directly image several types of plastic. Although current commercial MRI technology is not suitable for field use, existing single-sided MRI research systems could be adapted for use outside the laboratory and become an important tool for future monitoring of wild animals.

peer reviewed | The northern fulmar Fulmarus glacialis ingests a larger number of (micro)plastics than many other seabirds due to its feeding habits and gut morphology. Since 2002, they are bioindicators of marine plastics in the North Sea region, and data are needed to extend the programme to other parts of their distribution areas, such as the Arctic. In this study, we provide data on ingested plastics by fulmars collected in 1997 in Kongsfjorden, Svalbard. An extraction protocol with KOH was used and for half of the birds, the gizzard and the proventricular contents were analysed separately. Ninety-one percent of the birds had ingested at least one piece of plastic with an average of 10.3 (±11.9 SD) pieces. The gizzards contained significantly more plastics than the proventriculus. Hard fragments and polyethylene were the most common characteristics. Twelve percent of the birds exceeded the EcoQO value of 0.1 g.

peer reviewed | Monitoring plastic ingestion by marine wildlife is important for both characterizing the extent of plastic pollution in the environment and understanding its effect on species and ecosystems. Current methods to detect plastic in the digestive system of animals are slow and invasive, such that the number of animals that can be screened is limited. In this article, magnetic resonance imaging (MRI) is investigated as a possible technology to perform rapid, non-invasive detection of plastic ingestion. Standard MRI methods were able to directly measure one type of plastic in a fulmar stomach and another type was able to be indirectly detected. In addition to MRI, other standard nuclear magnetic resonance (NMR) measurements were made. Different types of plastic were tested, and distinctive NMR signal characteristics were found in common for each type, allowing them to be distinguished from one another. The NMR results indicate specialized MRI sequences could be used to directly image several types of plastic. Although current commercial MRI technology is not suitable for field use, existing single-sided MRI research systems could be adapted for use outside the laboratory and become an important tool for future monitoring of wild animals.

Contamination of aquatic ecosystems by hydrophobic organic contaminants (HOCs) is often assessed based on their concentrations in riverbed sediment and suspended particulate matter (SPM). However, total HOC concentration (CTOT) in sediment or SPM is of limited value for evaluating the exposure of benthic or pelagic organisms. The accessible HOC concentration (CAS) presents a useful parameter quantifying the overall pool of HOC in sediment or SPM available for fast partitioning to the water phase or biota. We applied a novel approach of ex situ sequential equilibrium partitioning with silicone elastomer sampler at a high sampler/SPM phase ratio to measure CAS of HOC in SPM from the Danube River. We compared CTOT and CAS in SPM and surface layer sediment collected at the same sites to evaluate whether HOC monitoring in the two matrices provides equivalent information on environmental quality. At most sites, there was a good agreement and correlation of organic carbon (OC)-normalised CTOT in SPM and sediment for polychlorinated biphenyls (PCBs) and the majority of organochlorine pesticides (OCPs). In contrast, CTOT of polycyclic aromatic hydrocarbons (PAHs) in SPM were up to a factor 10 lower in SPM than in sediment. Site-specific differences of OC-normalised CAS concentrations in SPM and sediments were observed for PCBs and OCPs, with accessibility mostly lower in SPM than in sediment. The highest accessibility in SPM was observed for PCBs, ranging between 15 and 30%. The accessibility of OCPs varied from 0 to 23%. SPM and riverbed sediment samples provide complementary but not mutually interchangeable information on HOC contamination. | acceptedVersion

Fertilizer, though one of the most essential inputs for increasing agricultural production, is a leading cause of nitrous oxide emissions from agriculture, contributing significantly to global warming. Therefore, understanding factors affecting farmers’ use of fertilizers is crucial to develop strategies to improve its efficient use and to minimize its negative impacts. Using data from 2528 households across the Indo-Gangetic Plains in India, Nepal, and Bangladesh, this study examines the factors affecting farmers’ use of organic and inorganic fertilizers for the two most important cereal crops – rice and wheat. Together, these crops provide the bulk of calories consumed in the region. As nitrogen (N) fertilizer is the major source of global warming and other environmental effects, we also examine the factors contributing to its overuse. We applied multiple regression models to understand the factors influencing the use of inorganic fertilizer, Heckman models to understand the likelihood and intensity of organic fertilizer (manure) use, and a probit model to examine the over-use of N fertilizer. Our results indicate that various socio-economic and geographical factors influence the use of organic and inorganic fertilizers in rice and wheat. Across the study sites, N fertilizer over-use is the highest in Haryana (India) and the lowest in Nepal. Across all locations, farmers reported a decline in manure application, concomitant with a lack of awareness of the principles of appropriate fertilizer management that can limit environmental externalities. Educational programs highlighting measures to improving nutrient-use-efficiency and reducing the negative externalities of N fertilizer over-use are proposed to address these problems. | 51480-51496