Chemically oxidative removal of polycyclic aromatic hydrocarbons (PAHs) in soil is related to their occurrence state. Whether the heterogeneity of natural organic matter has an effect on the occurrence of PAHs in soil and, if there is an effect, on the oxidative removal efficiency of PAHs remains unknown. In this study, the removal efficiencies of 16 priority PAHs aged in humic acids (HAs) of different soil aggregate fractions by various oxidants were investigated by combining soil fractionation and microreaction experiments. Results showed that the accumulations of PAHs in particulate HA (P-HA) and microaggregate occluded HA (MO-HA) mainly occurred in the early period of the aging time frame. In contrast, PAH accumulation in non-aggregated silt and clay associated HA (NASCA-HA) was relatively slow and tended to saturate in the late period of the aging time frame. The cumulative contents of PAHs throughout the entire aging period in MO-HA and NASCA-HA were significantly greater than that in P-HA. The aged PAHs in P-HA and NASCA-HA exhibited the highest and lowest removal efficiencies, respectively. This ranking was mainly governed by the molecular size and polarity of HAs. Sodium persulfate and potassium permanganate had the highest removal efficiencies in total PAHs in HAs, with average efficiencies of 85.8% and 79.1%, respectively, in P-HA. Hydrogen peroxide had the lowest degradation efficiency in PAHs. In particular, the degradation efficiency of total PAHs in NASCA-HA was lowered to 31.0%. PAH congeners in HAs showed a large difference in oxidative removal efficiency. Low-ring PAH was more easily degraded than medium- and high-ring PAHs, and in most treatments, fluoranthene and pyrene in the medium ring and benzo[a]pyrene in the high ring demonstrated higher efficiencies than other PAHs with the same number of rings. Our findings are useful in promoting the accurate and green remediation of PAH-contaminated soils.

The present work was done to explore the joint effect of Streptomyces pactum (Act12) and plant nutrients on phytoremediation of smelter-contaminated soils. The physiological indicators and phytoextraction indices of potherb mustard (Brassica juncea, Coss) grown in Act12 inoculated soil with or without Hoagland’s solution (H), humic acid (HA) and peat (PS) were evaluated. The results indicated that H, HA and PS acted synergistically with Act12, notably increasing chlorophyll and soluble protein contents and thereby promoting plant growth. Soil nutrient treatments reduced the antioxidant activities (PPO, CAT and POD) by 28.2–41.4%, 22.3–90.1% and 15.2–59.4% compared to control, respectively. Act12 and H treatments markedly facilitated plant to accumulate more cadmium (Cd) and zinc (Zn), but it was observed decreases when applied with HA and PS. Metal uptake (MU) values further indicated the differences in phytoextraction efficiency, i.e., H > PS > Control > HA. Taken together, Act12 combined with plant nutrients contributed to alleviating metal toxicity symptoms of plant. Hoagland’s solution and peat were highlighted in the present phytoextraction trial, and recommended as soil additives.

As an important part of extracellular secondary metabolites, extracellular polymeric substances (EPS) can play a significant role in protecting cells from the threat of exogenous substances, including nanoparticles (NPs). However, the regulation mechanisms of EPS secretion under NPs exposure remain largely unknown. This study investigated the signaling pathways and molecular responses related to EPS secretion of algae (Chlorella pyrenoidosa) upon the exposures to anatase and rutile TiO₂ NPs (nTiO₂-A and nTiO₂-R, respectively) at two similar toxic (20% and 50% of algal growth inhibition) concentrations. The results showed that EPS responded to nTiO₂ stress via excess secretion and compositional variation, and nTiO₂-A induced more EPS secretion than nTiO₂-R at similar toxicity concentrations. The up-regulation of the Ca²⁺ signaling pathway might play a greater role in promoting EPS secretion under nTiO₂-R exposure compared with nTiO₂-A exposure, while the significantly increased intracellular ROS could mainly account for the increased EPS secretion under nTiO₂-A exposure. The up-regulated genes related to biological synthesis and protein metabolism and the enhanced biosynthetic metabolism might be the direct causes of the increased EPS secretion. The increased ROS could have a greater effect on the amino acid metabolism and related genes upon the exposure to nTiO₂-A than nTiO₂-R to induce more EPS secretion. More serious membrane damage caused by nTiO₂-R than nTiO₂-A would affect the intracellular inositol phospholipid metabolism more severely, while the inositol phospholipid pathway and Ca²⁺ signaling pathway might agree and communicate with each other inherently to regulate EPS secretion upon nTiO₂-R exposure. The findings address the regulation mechanisms of algal EPS secretion under nTiO₂ exposure and provide new insights into algal bio-responses to nTiO₂ exposure.

The placenta is essential for sustaining the growth of the fetus. The aim of this study was to investigate the role of the placenta in MCLR-induced significant reduction in fetal weight, especially the changes in placental structure and function. Pregnant mice were intraperitoneally injected with MCLR (5 or 20 μg/kg) from gestational day (GD) 13 to GD17. The results showed MCLR reduced fetal weight and placenta weight. The histological specimens of the placentas were taken for light and electron microscopy studies. The internal space of blood vessels decreased obviously in the placental labyrinth layer of mice treated with MCLR. After the ultrastructural examination, the edema and intracytoplasmic vacuolization, dilation of the endoplasmic reticulum and corrugation of the nucleus were observed. In addition, maternal MCLR exposure caused a reduction of 11β-hydroxysteroid dehydrogenase type 2 (HSD11B2) expression in placentae, a critical regulator of fetal development. Several genes of placental growth factors, such as Vegfα and Pgf and several genes of nutrient transport pumps, such as Glut1 and Pcft were depressed in placentas of MCLR-treated mice, however nutrient transporters Fatp1 and Snat4 were promoted. Moreover, significant increases in malondialdehyde (MDA) revealed the occurrence of oxidative stress caused by MCLR, which was also verified by remarkable decrease in the glutathione levels, total antioxidant capacity (T-AOC) as well as the activity of antioxidant enzymes. Real-time PCR and western blot analysis revealed that GRP78, CHOP, XBP-1, peIF2α and pIRE1 were remarkable increased in placentas of MCLR-treated mice, indicating that endoplasmic reticulum (ER) stress pathway was activated by MCLR. Furthermore, oxidative stress and ER stress consequently triggered apoptosis which contributed to the impairment of placental development. Collectively, these results suggest maternal MCLR exposure results in reduced fetal body weight, which might be associated with ROS-mediated endoplasmic reticulum stress and impairment in placental structure and function.

In this study, 45 indoor dust samples and four particulate samples from air-conditioner filters were collected from four different indoor environments in Guangzhou, China, and the concentration and composition of organophosphate tri-esters (OPEs) and organophosphate di-esters (Di-OPs) were determined. Eight of the 10 target OPEs were detected in indoor dust at different detection frequencies (DFs), with tris(2-chloroisopropyl) phosphate and tris(2-chloroethyl) phosphate being the main components. Seven target Di-OPs were detected at different DFs, with diphenyl phosphate being the dominant compound. The total OPEs (∑8 OPEs) and total Di-OPs (∑7 Di-OPs) concentrations varied from 726 to 39,312 ng/g and 68.8–14,766 ng/g, respectively. The ∑8 OPEs concentrations in instrumental houses were significantly higher (p < 0.001) than in three other indoor environments. The varying strengths of the correlation between Di-OPs and their respective parent OPEs was suggestive of their emission sources (e.g., direct application, impurities in OPE formulas, and OPE degradation). The hazard index (HI) values of individual OPEs in residential house were lower than 1, the results suggested a limited human health risk from individual OPEs. However, the total HI value (∑HIs) of OPEs was approximately 1 based on a high exposure scenario and suggested a low risk for toddlers.

Pollutant gases and particulate matters (PM) from livestock facilities can affect the health of animals and farm workers and lead to great social environmental risks. This paper presents a comprehensive study on the characteristics of ammonia (NH₃), nitrogen oxides (NOₓ), sulfur dioxide (SO₂) and PM (including PM₂.₅ and PM₁₀) in a 100,000-bird manure-belt layer house in suburb Beijing for three typical seasons of summer, autumn and winter. Indoor air was sampled at an exhaust fan of the mechanically ventilated commercial house. The monitored indoor concentrations of NH₃, NOₓ, SO₂, PM₂.₅ and PM₁₀ were 3.7–5.0 mg m⁻³, 17–58 μg m⁻³, 0–11 μg m⁻³, 100–149 μg m⁻³ and 354–828 μg m⁻³, respectively. The indoor NH₃ concentrations were largely influenced by the manure removal frequency. The NOₓ and SO₂ were mainly sourced from the ambient air, and the NOₓ was also partly sourced from manure decomposition in summer. The indoor PM₂.₅ and PM₁₀ were largely sourced from the ambient air and the indoor manure, respectively. The abundant indoor NH₃ caused significantly higher NH₄⁺ concentration in the indoor PM₁₀ (7.98 ± 9.04 μg m⁻³) than that in the ambient PM₁₀ (3.48 ± 3.52 μg m⁻³). Secondary inorganic ions (SO₄²⁻, NO₃⁻ and NH₄⁺) totally contributed 5.7% and 14.6% to the indoor and ambient PM₂.₅, respectively; they contributed 2.8% and 8.9% to the indoor and ambient PM₁₀, respectively. Organic carbon was the main component of the PM and accounted for 26.6% and 41.5% of the indoor PM₂.₅ and PM₁₀, respectively. Heavy metal elements (Zn, Cu and Cr) were likely transported from feed to manure and finally accumulated in the PM. Given the high emission potential, the air pollutants from animal production suggested potential risks for human health.

Atrazine contamination is of great concern due to its widespread occurrence in shallow lakes. Here, the distribution and degradation of atrazine in acidic and alkaline lake systems were investigated. Meanwhile, the bacterial communities in different sediments and the effects of environmental factors on atrazine-degrading bacteria were evaluated. In the lake systems without plants, atrazine levels in sediment interstitial water reached peak concentrations on the 4th d. More than 90% of atrazine was then degraded in all sediment interstitial water by day 30. Meanwhile, the degradation rate of atrazine in alkaline sediments was faster than that in acidic sediments. Values of hydroxylated metabolites in the acidic lake sediments tended to be greater. Moreover, the amounts of Proteobacteria, Actinobacteria, Firmicute, Nitrospinae, Aminicenantes, Ignavibacteriae and Saccharibacteria in acidic Tangxunhu Lake sediments were significantly different from alkaline Honghu Lake sediments, while the amounts of Cyanobacteria and Saccharibacteria in sediments treated with atrazine were significantly greater than those in sediments without atrazine (P < 0.05). Notably, pH was the most relevant environmental factor in the quantitative variation of atrazine-degrading bacteria, including in Clostridium-sensu-stricto, Pseudomonas, Comamonas and Rhodobacter. The Mantel test results indicated that the degradation of atrazine in different sediments was mainly affected by the sediment physicochemical properties rather than by the addition of atrazine and the cultivation of hydrophytes.

Evaluation of the effectiveness of best management practices for reducing nitrate leaching in agricultural systems requires detailed water and nitrogen (N) budgets. A 3-year field experiment using 15 auto-weighing lysimeters was set up to quantify nitrate leaching, crop evapotranspiration (ET), and N and water use efficiencies within an intensive wheat-maize rotation system in the Northern China Plain. The lysimeter consists mainly of the following: (1) high-resolution weighing cells; (2) ceramic solution samplers for soil solutions collection; and (3) circular stainless steel leaching trays for collecting seepage water. Two N fertilizer types were applied at two rates (150 and 225 kg N hm⁻² for each crop) with no-N applied as the control. The N fertilizer types were monotypic un-coated urea and a blend product with controlled-release urea (CRU) and un-coated urea. The results indicate that when compared with un-coated urea at the same application rate, the blend product greatly improved water and N use efficiencies with significant increase in yields and crop ET as well as reduction of nitrate accumulation and leaching in the soil profile (p < 0.05). This was mostly because the blend product consistently supplied N to meet crop demands over the entire growth season. The study implied that effective best management practices to control nitrate leaching should be based on technically sound fertilization and irrigation schemes in terms of timing, rate, and fertilizer type to suit site specific conditions.

Colloids in groundwater or geological barriers generally play a key role in the migration of special nuclides. Adsorption characteristics of strontium were investigated on porous media in the presence of colloidal Fe(OH)₃ from the Beishan Site, the only high-level radioactive waste disposal site candidate in China. The effects of colloid amounts, solid contents, and pH were determined and studied by batch texts. The results revealed that the presence of colloidal Fe(OH)₃ in porous media contributed to promotion of the sorption effect, and the influencing factors had a significant impact on the adsorption process. The sorption ability increased with increasing colloid amount when the equilibrium time was approximately 10 h under an optimal solid-liquid ratio of 20 g L⁻¹. The sorption effect in alkaline conditions was better than that under acidic conditions. The sorption kinetics indicated that the strong chemical interaction and/or surface complexation contributed primarily to strontium sorption. The sorption isotherms and model fitting revealed that the sorption of strontium onto porous media in the presence of colloidal Fe(OH)₃ was a monolayer adsorption, and the presence of colloidal Fe(OH)₃ is an important factor that greatly influences the removal of strontium from aqueous solutions. These findings provide useful information for the treatment of strontium in radioactive waste disposal sites.

Atmospheric pollution could significantly alter tree growth independently and synergistically with meteorological conditions. North China offers a natural experiment for studying how plant growth responds to air pollution under different meteorological conditions, where rapid economic growth has led to severe air pollution and climate changes increase drought stress. Using a single aspen clone (Populus euramericana Neva.) as a ‘phytometer’, we conducted three experiments to monitor aspen leaf photosynthesis and stem growth during in situ exposure to atmospheric pollutants along the urban-rural gradient around Beijing. We used stepwise model selection to select the best multiple linear model, and we used binned regression to estimate the effects of air pollutants, atmospheric moisture stress and their interactions on aspen leaf photosynthesis and growth. Our results indicated that ozone (O₃) and vapor pressure deficit (VPD) inhibited leaf photosynthesis and stem growth. The interactive effect of O₃ and VPD resulted in a synergistic response: as the concentration of O₃ increased, the negative impact of VPD on leaf photosynthesis and stem growth became more severe. We also found that nitrogen (N) deposition had a positive effect on stem growth, which may have been caused by an increase in canopy N uptake, although this hypothesis needs to be confirmed by further studies. The positive impact of aerosol loading may be due to diffuse radiation fertilization effects. Given the decline in aerosols and N deposition amidst increases in O₃ concentration and drought risk, the negative effects of atmospheric pollution on tree growth may be aggravated in North China. In addition, the interaction between O₃ and VPD may lead to a further reduction in ecosystem productivity.