Microglia (MG) are resident phagocytes in the brain responsible for neuronal maintenance. The regulation of MG necroptosis is required for protecting neurons during neurodegenerative diseases. Therefore, this study proposed to elucidate the molecular mechanisms underlying microglia necroptosis during long-time apoptotic stimuli (lipopolysaccharide, LPS). The protective role of plasmalogens (PLS) was also investigated against LPS insult in MG cells (including BV2 and MG6 cell lines). LPS produced time-dependent decreases in the survival of BV2 and MG6 cells mediated by the caspase signaling pathway. Interestingly, MG death was mediated by caspase-8 and 9 signaling pathways suggesting that MG necroptosis was actively attributed to long-time LPS treatment through intrinsic and extrinsic pathways. Notably, caspase signaling was markedly inhibited in the PLS-pretreated cells; thereby, PLS were capable of maintaining the MG cell population and inhibit the MG necroptosis against the longtime of LPS administration via its antioxidant and anti-inflammatory properties.

The present study illustrates a holistic account of zooplankton community dynamics in relation to physicochemical variables in the tidal creeks of Indian Sundarbans estuarine system. Eleven water variables and zooplankton samples were collected in a monthly basis from the six sampling locations between July 2016 and June 2018. Out of the 11 water parameters, seven parameters (temperature, salinity, dissolved oxygen, turbidity, PO₄ − P, NO₃ − N, and NO₂ − N) varied significantly (p ≤ 0.05) among seasons. A total of 63 zooplankton taxa were recorded with the predominance of Copepoda. Permutational multivariate analysis of variance (PERMANOVA) depicted the significant variations of zooplankton population both spatially (F = 2.313; p = 0.001) and temporally (F = 6.107; p = 0.001). Out of the 41 species of Copepoda recorded, 14 species (Paracalanus parvus, Parvocalanus dubia, Bestiolina similis, Acrocalanus gibber, A. gracilis, Acartia erythraea, A. spinicauda, Pseudodiaptomus serricaudatus, P. annandalei, P. aurivilli, Oithona brevicornis, O. similis, Longipedia weberi and Microsetella norvegica) were indicated as “characterizing species” in the creek environment, which highlighted the euryhaline nature as well as broad range of thermal tolerance of these species. Acrocalanus longicornis, Eucalanus crassus, Candacia bradyi, and Acartia sewelli were specifically found in pre-monsoon; Euterpina acutiformis, Clytemnestrata rostrata, Acartia plumosa, and Canthocalanus pauper were specific to post-monsoon season, whereas Mesocyclops leuckarti, Temora turbinata, and Temora discaudata were found only during monsoon. Thus, it explains the limited period of existence, highlighting the temporal shift in species abundance in the creeks. Overall, the calculated mean value of α-diversity (dʹ = 4.07; H' = 2.31) indicated a “good” zooplankton diversity in the creeks. The biota-environmental relationship following canonical correspondence analysis revealed that variables, viz., water temperature, turbidity, and NO₃ − N, were the explanatory variables during monsoon; pH and DO in post-monsoon; PO₄ − P, SiO₄ − Si, and salinity in pre-monsoon that explained the variations of zooplankton community compositions in the creeks. The variables (PO₄ − P, NO₃ − N, and SiO₄ − Si) that are correlated to the zooplankton community implied their indirect effect on the distribution of zooplankton by supporting the phytoplankton succession and production in the mangrove-dominated creeks.

In this study, we evaluated the residual levels and distribution characteristics of 10 organochlorine pesticides (OCPs) in the soil of agricultural lands (paddy field, vegetable field, and orchard, n = 150) nationwide. As a result of the investigation, 3–10 types of OCPs were detected in combination in 144 locations (96%) of the total 150 locations. Among those OCPs, dichlorodiphenyltrichloroethane (DDT) and endosulfan were detected in high amounts. The geometric mean concentrations of DDT and endosulfan were ∑DDT 0.31 ng g⁻¹ (ND-2187.18 ng g⁻¹) and ∑endosulfan 0.33 ng g⁻¹ (ND-312.68 ng g⁻¹), respectively. OCP concentrations were generally higher in orchards than in other agricultural fields due to a large amount of pesticides used. DDT in two orchards was detected at 2187.18 ng g⁻¹ and 1061.78 ng g⁻¹, exceeding the levels stated in the Canadian DDT Soil Quality Guidance (SQG, 700 ng g–¹). The result of applying the isomer ratio to evaluate the recent or past use of DDT, endosulfan, and hexachlorocyclohexane (HCH), which had a high detection rate, showed that it could be attributable to historical usage. DDT and HCH showed statistically similar residual distribution patterns; however, endosulfan did not show a clear correlation with DDT and HCH due to the effect of its recent use. Thus, the results of evaluation of OCP residual levels and distribution characteristics can be used for the soil management policy of OCPs in agricultural lands in Korea.

The potential association between maternal exposure to PM₁₀ ranging from 3 months prior to conception to the end of the early trimester and the risk of anorectal atresia/stenosis in offspring has not been established. Thus, we determined the association between maternal exposure to PM₁₀ and risk of anorectal atresia/stenosis in offspring in this study. We recruited 713 patients including 480 male and 233 female with anorectal atresia/stenosis and 7950 randomly selected healthy offspring from the Maternal and Child Health Certificate Registry of Liaoning Province and delivered between 1 January 2010 and 31 December 2015. Monthly PM₁₀ concentrations were retrieved from the Environment Protection Bureau of each city in Liaoning Province. We established a multivariable logistic regression model to calculate the adjusted odds ratios (ORs) and 95% confidence intervals (CIs). Maternal exposure to PM₁₀ was associated with an increased risk for anorectal atresia/stenosis in offspring during the 3 months prior to conception (per 10 μg/m³ increment: OR = 1.15, 95% CI = 1.03–1.20; per SD [27 μg/m³] increment: OR = 1.33, 95% CI = 1.09–1.63) and the first trimester (per 10 μg/m³ increment: OR = 1.08, 95% CI = 1.00–1.17; per SD [28 μg/m³] increment: OR = 1.26, 95% CI = 1.01–1.57). Evaluation of the association with a shorter exposure window (1 month) revealed a positive association between anorectal atresia/stenosis and PM₁₀ from the 3rd month prior to pregnancy to each month of the 1st trimester. Maternal exposure to PM₁₀ 3 months prior to conception and during the 1st trimester was associated with an increased risk of anorectal atresia/stenosis in the offspring. Future perspective cohort studies are needed to confirm our findings.

An excessive amount of phosphate (PO₄³⁻) released from domestic wastewater treatment plant effluent (DWTPE) may trigger eutrophication of water causing a degradation of healthy aquatic ecosystem. Even though the PO₄³⁻ ions can be removed from aqueous solution with an adsorption technique using the low-cost adsorbent, the adsorption kinetics of PO₄³⁻ removal must be understood. The bed depth service time (BDST), Thomas and modified mass transfer factor (MMTF) models were used to investigate the adsorption kinetics of PO₄³⁻ removed from DWTPE onto the waste mussel shell (WMS) applied to hybrid plug flow column reactor (HPFCR). Dynamic adsorption capacity of WMS described by the new modified BDST model is shown to increase with increasing of the plug flow column (PFC) bed. The analysis of mass transfer behavior described using the Thomas model is able to predict the performance of HPFCR at certain depths of the PFC bed. The use of the MMTF models could be useful to describe the real difference between the behaviors of film mass transfer and porous diffusion. The resistance of PO₄³⁻ mass transfer depending on porous diffusion has been verified to provide a contribution in the development of advanced WMS adsorbent for enhancing the HPFCR performance in the future.

Sulfate-reducing anaerobic ammonium oxidation (SRAO) is one of the unique biochemical reactions involving the use of ammonium as the electron donor and sulfate as the electron acceptor. Bacillus cereus (named SUD-1) was isolated from industrial wastewater under an optimized anaerobic acclimation procedure for 14 days which is capable to be mixotrophic. The isolate was identified using the 16 S rRNA sequencing as Bacillus cereus FDAARGOS_798 with optimum growth pH and temperature of 7 and 30 ± 2 °C, respectively. The SUD-1 reached the maximum removal efficiency of 67% (NH⁺₄) and 80% (SO²⁻₄) in a closed anaerobic batch for 10 days. Its optimum pH, temperature, isolate volume, and (NH₄)₂SO₄ substrate concentration were 8, 30 °C, 5% (v/v), and 0.1 g/L, respectively. The result has shown the pronounced performance of SUD-1 strain as a novel biomaterial to simultaneously remove ammonium and sulfate which is an unconventional reaction and thus indicates a good potential for application in real wastewater treatment plants.

The effect of financial development on carbon emissions is a hot topic. Although some researches study the heterogeneous impacts of financial development on carbon emissions at the country level, few paper has investigated their heterogeneous relations within the same country. This paper, applying geographically and temporally weighted regression (GTWR), studies the spatial–temporal heterogeneity of the impacts of financial development on carbon emissions across China’s 30 provinces from 2003 to 2017. The results show that financial development proxied by bank credit indicators curbs carbon emissions in most provinces most of the time, while that proxied by stock market indicator exhibits nonlinear relationships in most provinces, such as U-shaped, inverse U-shaped, and inverse N-shaped. The paper concludes first that financial development proxied by different indicators may exert varied impacts on carbon emissions. Second, the impact of financial development on carbon emissions shows great heterogeneity among different provinces and different years: it may be curbing or increasing, and even it is curbing, its curbing effects differ greatly across provinces and years. Third, the impact of financial development on CO2 is not always monotonic; instead, it may be nonlinear. Regional segmentation of financial markets may explain the heterogeneity. Some policy suggestions are also given.

This study aimed to elucidate the boundaries of ammonia and organic loading rates that allow for nitritation in continuous flow phosphorylated-polyvinyl alcohol entrapped cell reactors and to clarify the community structure of microorganisms involving nitrogen transformation in the gel bead matrices. At operating bulk dissolved oxygen concentration of 2 mg/L, nitritation was accomplished when the total ammonia nitrogen (TAN) loading rate was ≥ 0.3 kgN/m³/d. At TAN loading rates of ≤ 0.2 kgN/m³ /d, complete oxidation of ammonia to nitrate took place. Nitritation performance dropped with increased chemical oxygen demand (COD) loading rates indicating limitation of nitritation reactor operation at some COD loading conditions. 16S rRNA gene amplicon sequencing revealed that the uncultured Cytophagaceae bacterium, Arenimonas, Truepera, Nitrosomonas, Comamonas, unclassified Soil Crenarchaeotic Group, and uncultured Chitinophagaceae bacterium were highly abundant taxa in the reactors’ gel bead matrices. qPCR with specific primers targeting amoA genes demonstrated the coexistence of ammonia-oxidizing bacteria (AOB), ammonia-oxidizing archaea, and Comammox in the gel bead matrices. AOB was likely the main functioning ammonia-oxidizing microorganisms due to the amoA gene being of highest abundance in most of the studied conditions. Nitrite-oxidizing microorganisms presented in less relative abundance than ammonia-oxidizing microorganisms, with Nitrobacter rather than Nitrospira dominating in the group. Results obtained from this study are expected to further the application of nitritation entrapped cell reactors to real wastewater treatment processes.

The association between trade, financial development, consumption of renewable energy, environmental quality, foreign direct investment, and economic growth is important for sustainable growth and environmental strategies. Hence, this research unveils this association in selected low- and high-income economies from 1996 to 2020. Unlike most of the previous literature, this study uses a composite environmental quality index, a composite financial development index, and a composite trade share measure to better represent environmental quality, financial development, and trade openness, respectively. The Continuously Updated Fully Modified and Continuously Updated Bias Corrected estimators along with the Dumitrescu Hurlin causality method are utilized to scrutinize the nature of the linkage between the modeled variables. The long-run estimation provided that consumption of renewable energy and environmental quality augment economic growth in high-income nations, while both these variables do not contribute to the economic growth in low-income countries. Financial development upsurges economic growth in high- as well as low-income nations. Interestingly, trade openness boosts economic growth in high-income countries, while in low-income countries, it obstructs economic growth. In causal linkage, the conservation hypothesis for low-income countries and the feedback hypothesis for high-income countries are confirmed in the context of consumption of renewable energy and economic growth association. The supply-leading hypothesis for low-income countries and the feedback hypothesis for high-income countries are supported regarding the financial development–economic growth nexus. Moreover, one-way causality from growth to environmental quality and bidirectional causality between environmental quality and economic growth for low- and high-income countries are established, respectively. Lastly, exhaustive environmental and economic policies are directed.

Low-cost adsorbent, pine bark biochar (PBB) from the forest residue, was produced and applied to remove tetracycline (TC) from aqueous solution via adsorption pathway. The PBB, hence obtained, was modified using aqueous ferric and ferrous ion solutions to obtain magnetic pine bark biochar (M-PBB). Batch adsorption experiments were conducted to examine the adsorption of TC by PBB and M-PBB in the variation of pH, contact time, dosage, and temperature. The adsorbents were characterized by SEM/EDX, TGA, and pHₚzc. The adsorption mechanism was evaluated by fitting Langmuir, Freundlich, Temkin, and Dubinin-Radushkevich (D-R) isotherms model. Also, the experimental data were analyzed by kinetics models (pseudo-first-order, pseudo-second-order, intra-particle diffusion, and Elovich) and thermodynamics. The maximum adsorption capacity (qₘ) of M-PBB was 15.3 mg/g from the experiment at pH 6. A high correlation coefficient (R² ≈ 0.9) of Freundlich isotherm postulated multi-layer adsorption of TC on M-PBB at pH 6. The kinetic studies showed that the pseudo-first-order was more suitable for representing the adsorption of TC molecules on the surface. The thermodynamic analysis was showed that the adsorption process is favorable, spontaneous, and endothermic at studied temperatures. M-PBB demonstrated a potential for removal of TC from water as a low-cost and convenient adsorbent.