Lemna minuta Kunth was used to remove Cr(VI) from aqueous solutions, and some of the mechanisms involved in this process were analyzed. In addition, the cellular signaling mediated by phospholipase D activity as well as antioxidant responses was also evaluated during the process. Cr(VI) removal efficiencies were 40% for 0.5 mg/L, after 24 h, and up to 18% at metal concentrations as high as 5 mg/L. Removal mechanisms displayed by these macrophytes include bioadsorption to cell surfaces and, to a greater extent, Cr internalization and bioaccumulation within cells. Inside of them, Cr(VI) was reduced to Cr(III), a less toxic form of this metal. At the first hours of Cr(VI) exposure, plants were able to sense chromium, activating membrane signal transduction pathways mediated by phospholipase D and phosphatidic acid. Moreover, an increase in the activity of antioxidant enzymes such as superoxide dismutases and peroxidases was observed in the same time. These and other components of the antioxidant defense system would help to reduce the stress generated by the metal. The toxicity of the products formed during the removal process was assessed through Lactuca sativa L. and AMPHIAGU test. It was evidenced that Cr(VI) phytoremediation process by L. minuta plants did not generate acute toxicity neither for L. sativa seeds nor for embryos of Rhinella arenarum (Hensel, 1876). Thus, L. minuta plants could be considered as valuable species for the treatment of waters contaminated with Cr(VI).

To evaluate Cu and Zn levels in bladder cancer (BC) patients and their relationship with expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor 1 (HIF-1). Plasma levels of Cu and Zn were determined in 66 transitional bladder cell carcinoma patients (BC group) and 60 matched controls. The concentration of Cu and Zn as well as the expressions of both VEGF and HIF-1 were also estimated in cancerous and non-cancerous bladder tissues in the BC group. The results showed that plasma Cu and Cu/Zn ratio were significantly higher in BC group when compared with the control group. In contrast, the plasma Zn in BC group was significantly lower than in the controls. Comparing levels of Cu and Zn in cancerous and non-cancerous bladder tissues among the BC group indicated a significantly higher Cu levels in the cancerous tissues, while Zn levels was significantly lower. There were higher expressions of both VEGF and HIF-1 in the cancerous samples. Moreover, the Cu concentration in cancerous tissues was significantly correlated with expressions of VEGF and HIF-1. Logistic regression analysis revealed that the increase in plasma Cu/Zn ratio and plasma Cu and the decrease in plasma Zn may be risk factors for development of bladder cancer. We concluded that alteration of plasma and bladder tissue levels of both Cu and Zn is correlated with pathogenesis of bladder cancer. The increase in Cu level in cancerous tissues of BC group has an important role in angiogenesis in bladder cancer cells.

National key towns are one of the most important units for mitigating China’s carbon emissions from urbanisation, industrialisation and agricultural modernisation under China’s rural revitalisation strategy. The rise in the carbon footprint (CF) corresponding to increasing populations and energy consumption in national key towns has not been underestimated. This paper aims to explore the impact of local resident energy consumption on the degree of coordination between ecology and the economy. To achieve this objective, the carbon pressure index (CPI), carbon occupancy index (COI) and eco-economic coordination index (EECI) are constructed by applying the CF, carbon carrying capacity (CC) and carbon deficit (CD) of Caijiapo Town from 2012 to 2017. Furthermore, the relationship between the CF, economic growth and per capita disposable income is described based on the decoupling index (DI). The results reveal that, apart from a decline in 2014, the per capita CF of Caijiapo Town has increased, with an average annual growth rate of 16.80%. The CF of different energy sources shows that the CF of natural gas is much larger than those of raw coal, oil and electricity. The DI between GDP, the per capita disposable income of urban and rural residents and the resident energy CF first decreases and then increases. The DI between GDP, the per capita disposable income of urban residents and the resident energy CF is 1.09 and 1.72, and urban residents are the main contributors to the resident energy CF from 2015 to 2017. The CPI and COI show an upward trend, and the eco-economic coordination index (EECI) of Caijiapo Town undergoes a change from poor coordination (grade II) to worst coordination (grade I) from 2012 to 2017. Therefore, the following suggestions are proposed: the CC should be treated as a constraint, energy utilisation should be improved, residents’ consumption structure should be optimised, differentiated policies should be implemented for the energy consumption of urban and rural residents and a spatial planning system and operational mechanism should be established that combines multiple regulations into a single regulation.

This work evaluates the impact of the different concentrations of Fe₃O₄ on nitrate removal and organic matters utilization in the sequencing batch reactors (SBRs) using the sludge alkaline digestion supernatant as external sludge carbon source. Results indicated that the optimal concentration of Fe₃O₄ was 1 g/L for enhancing denitrification with NO₃⁻-N removal efficiency of 93.13% (up to a 11.93% increase) and without NO₂⁻-N accumulation after 18 days. The changes of soluble chemical oxygen demand (SCOD), protein, and carbohydrate during denitrification process were analyzed to gauge the utilization of sludge fermentation products by denitrifiers. The SCOD was consumed for organisms involved in NO₃⁻-N removal and the Fe₃O₄ could promote the utilization of carbohydrate better than protein by denitrifiers during denitrification process. Denitrification rate (VDN) and the nitrate-to-nitrite transformation ratio (NTR), as the kinetics parameters, were also investigated in different concentrations of Fe₃O₄.

This work aims to utilize fly ash from a thermal power station for melanoidin reduction from distillery effluent by adsorption. To accomplish this, coal fly ash was modified through chemical treatment and was then tested for melanoidin adsorption as a function of various melanoidin concentrations, contact time, and pH. The specific novelty of this study is the evaluation of coal fly ash as a low-cost adsorbent for melanoidin removal. Furthermore, the simulation study was carried out using Aspen ADSIM software in order to optimize the commercial usage of the prepared adsorbent. The main results achieved include the maximum removal efficiency of 84% which was reached at initial melanoidin concentration of 1100 mg L⁻¹ (5% dilution), pH 6, and a contact time of 120 min. The Langmuir and Freundlich isotherm models were used to evaluate adsorption isotherms. The maximum adsorption capacity of 281.34 mg/g was observed using the Langmuir isotherm. Furthermore, pseudo-first- and pseudo-second-order and intra-particle diffusion models were used to fit adsorption kinetic data. The pseudo-second-order was best describing the adsorption kinetic with a faster kinetic rate of 0.142 mg g–1 min⁻¹. CFA (coal fly ash) after acidic activation resulted in a slightly higher surface area, average pore volume, and pore size. The maximum breakthrough time and adsorbent saturation time were achieved at initial melanoidin concentration of 1 mol/lit, bed height of 2.5 m, and flow rate of 50 lit/min.

For geological carbon sequestration, the reaction of aqueous CO₂ with silicate rock permits carbonate formation, achieving permanent carbon sequestration. The fractures available in silicate rock provide significant surface area for the precipitation of carbonates. The experiments were performed in a batch tubular reactor under diffusion-limited condition, with a special arrangement of a narrow tube filled with a 2800 g/L dunite slurry. The tube was kept open from the top, standing vertically filled with a CO₂-rich bulk solution under 1 barg CO₂ and temperatures ranging from 25 to 75 ᵒC for 7–30 days. After 7 days of the experiment, magnesite precipitation was seen inside the tube and the precipitation was continued for up to 30 days. The magnesite precipitation was identified by micro-Raman spectroscopy, X-ray diffraction, and scanning electron microscopy. Additionally, SiO₂ formation was seen in relative close vicinity to the magnesite precipitation. The precipitation on the surface of silicate rock might cover the fractures and pore spaces available, which may over time reduce the dissolution rate of dunite. Graphical Abstract

The present study is the first report for studying the toxic effects of butralin herbicide on COX2, BAX, and Bcl2 gene expression, oxidative stress, and liver damage in female rats. Female rats were received butralin in drinking water for 28 days at concentration 4.16, 312, and 3120 mg/L that corresponded to the acceptable daily intake (ADI), no observed adverse effect level (NOAEL), and 10 NOAEL, respectively. Butralin decreased body weights and increased relative liver weight of female rats exposed to high dose. It caused significant elevation in liver function enzymes, lipid peroxidation, and reactive oxygen species (ROS). Antioxidant enzymes were decreased in liver tissue by increasing the dose. Butralin induced over-expression in the apoptotic related genes including COX2, BAX, and Bcl2 and pathological alteration in the liver of female rats especially at a high dose. It can be concluded that butralin induced oxidative damage and liver injure. The mechanism of damage could be due to generate reactive oxygen species, and increase lipid peroxidation that causes over-expression in the apoptotic related genes including COX2, BAX, and Bcl2. From the Benchmark dose (BMD) approach, there is dose-dependent manner in body weight, AST, ALT, and ALP, and ALT is a very sensitive parameter.

A detailed investigation was carried out to assess the concentration of near-road traffic-related air pollution (TRAP) using a dispersion model in Muscat. Two ambient air quality monitoring (AQM) stations were utilized separately at six locations near major roadways (each location for 2 months) to monitor carbon monoxide (CO) and nitrogen oxides (NOx). The study aimed to measure the concentration of near-road TRAP in a city hot spots and develop a validated dispersion model via performance measures. The US Environmental Protection Agency (US EPA) Line Source Model was implemented in which the pollutant emission factors were obtained through Comprehensive Modal Emission Model (CMEM) and COmputer Programme to calculate Emissions from Road Transport (COPERT) model. Traffic data of all vehicle categories under normal driving conditions including average vehicle speed limits and local meteorological conditions were included in the modeling study. The analysis of monitoring data showed that hourly (00:00 to 23:00) concentrations of CO were within the US EPA limits, while NOx concentration was exceeded in most locations. Also, the measured pollutant levels were consistent with hourly peak and off-peak traffic volumes. The overall primary statistical performance measures showed that COPERT model was better than CMEM due to the high sensitivity of CMEM to the local meteorological factors. The best fractional bias (0.47 and 0.39), normalized mean square error (0.44 and 0.50), correlation coefficient (0.64 and 0.70), geometric mean bias (1.07 and 1.57), and geometric variance (2.00 and 2.32) were obtained for CO and NOx, respectively. However, the bootstrap 95% CI estimates over normalized mean square error, fractional bias, and correlation coefficient for COPERT and CMEM were found to be statistically significant from 0 in the case of combined model comparison across all the traffic locations for both CO and NOx. In overall, certain roads showed weak performance mainly due to the terrain features and the lack of reliable background concentrations, which need to be considered in the future study.

Streams and surrounding terrestrial ecosystems are closely linked by numerous resource subsidies including anthropogenic subsidies which are increasingly entering streams due to intensive human activities. Also, streams are threatened by stressors such as glyphosate—the most widely used herbicide worldwide. However, the ecological consequences of anthropogenic subsidies and glyphosate on freshwaters are not fully understood. Here, we deployed leaf litter (Cinnamomum camphora) bags containing neither, either, or both treatments of anthropogenic carrion subsidy (chicken meat) and glyphosate (coated in agar) in four streams, which had different land use (i.e., forest, village, and suburban) in Huangshan, Anhui Province, China. We aimed to investigate the individual and combined effects of anthropogenic carrion subsidy and glyphosate on macroinvertebrates in streams and whether these effects differ with land use change. Macroinvertebrate communities significantly differed among streams: biodiversity index and total taxon richness were highest in village streams and lowest in suburban stream. Overall effects of carrion subsidy and glyphosate on macroinvertebrates were not significant. However, several taxa were affected in one or more streams by the individual or combined effects of carrion subsidy and glyphosate, indicating the importance of local community structure and physical habitats in driving the response of macroinvertebrates to carrion subsidy and glyphosate. Collectively, these results imply that the effects of carrion subsidy and glyphosate on macroinvertebrates are site-specific, and future studies should cover more streams and last longer time to better understand the ecological mechanisms driving such pattern.

The disposal of bulky low-concentration rare earth solutions (usually ≤ 200 mg L⁻¹) is difficult and it can easily lead to the waste of rare earth resources. The precipitant separation method is a simple and effective technique that is commonly used for rare earth recovery, but the application of biological component precipitants is rarely reported. In this study, the effects of the precipitation of low concentrations of rare earth ions by excess sludge humic-like acids were evaluated. Scanning electron microscopy with energy-dispersive spectroscopy (SEM/EDS) and infrared (IR) spectroscopy revealed that the addition of humic-like acids to low-concentration rare earth solutions could produce a flocculent precipitate. The precipitation rate was more than 89%. The content of rare earth metals such as Nd, Y, and La in the rare earth precipitate reached 23.72%, and the elution rate of 1.25 mol L⁻¹ hydrochloric acid was 90.50%. It was concluded that the humic-like acids might contain many functional groups capable of adsorbing rare earth ions. It was inferred that the possible mechanism might be that rare earth ions were precipitated by a series of net catch, ion exchange, and adsorption processes. These findings provide a reference for the future recovery of rare earth resources.