Cardiovascular diseases (CVD) are leading global health issue. More studies have linked indoor air pollution from solid fuel usage to hypertension risk, a leading risk factor for CVD. We conducted a systematic review and meta-analysis of observational studies assessing the relationship of indoor air pollution from solid fuel with hypertension risk. Using a protocol standardized a priori, two independent reviewers searched PubMed, the Cochrane Library, Ovid MEDLINE, Web of Science and EMBASE for available studies published before Dec.1, 2019. A random effects model was used to analyse the pooled results. Out of 3740 articles, 47 were reviewed in depth and 11 contributing to this meta-analysis. The use of household solid fuel was significantly associated with an increased risk of hypertension (OR = 1.52, 95% CI = 1.26 to 1.85). The smoking-controlled group (OR = 2.38, 95% CI = 1.58 to 3.60) had greater effect size of hypertension than the uncontrolled group (OR = 1.11, 95% CI = 1.10 to 1.11). These findings implicate that indoor air pollution from solid fuel may be an important risk factor for hypertension.

Polycyclic aromatic hydrocarbons (PAHs) in soil are not only detrimental to environment but also to human health. Double dielectric barrier discharge (DDBD) plasma reactor used for the remediation of pyrene contaminated soil was studied. The performance of DDBD reactor was optimized with influential parameters including applied voltage, type of carrier gas, air feeding rate as well as pyrene initial concentration. The analysis of variance (ANOVA) results showed that input energy had a great effect on pyrene remediation efficiency followed by pyrene initial concentration, while, the effect of air feeding rate was insignificant. More specifically, the remediation efficiency of pyrene under air, nitrogen and argon as carrier gas were approximately 79.7, 40.7 and 38.2% respectively. Pyrene remediation efficiency is favored at high level of applied voltages and low level of pyrene initial concentration (10 mgkg⁻¹) and air feeding rate (0.85 L/min). Moreover, computation of the energy efficiency of the DDBD system disclosed that an optimal applied voltage (35.8 kV) and higher initial pyrene concentration (200 mgkg⁻¹) favored the high energy efficiency. A regression model predicting pyrene remediation under DDBD plasma condition was developed using the data from a face-centered central composite design (FCCD) experiment. Finally, the residual toxicity analysis depicted that the respiratory activity increased more than 21 times (from 0.04 to 0.849 mg O₂ g⁻¹) with a pyrene remediation efficiency of 81.1%. The study demonstrated the DDBD plasma technology is a promising method not only for high efficiency of pyrene remediation, but also recovering biological function without changing the physical-chemical properties of soil.

The prevalence of pharmaceuticals and personal care products (PPCPs) in lotic habitats is increasing, with the main source of these contaminants being effluent from waste water treatment works (WwTW). There is still much uncertainty about the impacts of these PPCPs at environmentally relevant concentrations and their potential effects on aquatic ecology. Behaviour is a sensitive endpoint which can help evaluate possible population level effects from changes in physiology. This paper evaluates the effects of WwTW effluent on a range of behaviours in the freshwater invertebrate, Gammarus pulex. Effluent taken from the outflow of two WwTW in southern England was used in the study. Behavioural analyses, namely feeding rate, phototaxis, activity, velocity and precopula pairing, were measured in G. pulex following a period of one and three weeks after exposure to a 50% or 100% effluent and a control. Mortality remained very low throughout the 3 week experiment (0–10%, n = 20) and no significant changes in moulting frequency were observed (p > 0.05). No significant effects on feeding or velocity or phototaxis following 3 weeks of effluent exposures were observed (p > 0.05). However, significant reductions were observed in the overall activity over 3 weeks across which appeared to be exacerbated by exposure to effluents. Interestingly, males exposed for 3 weeks to WwTW effluent re-paired with unexposed females significantly faster (4-6x) than control animals. This result was consistent between the effluents taken from the two WwTW. The implications of these behavioural changes are currently unknown but highlight the need for a varied set of tools to study the behavioural changes in wildlife.

Given the ubiquitous presence of microplastics in aquatic environments, an evaluation of their toxicity is essential. Microplastics are a heterogeneous set of materials that differ not only in particle properties, like size and shape, but also in chemical composition, including polymers, additives and side products. Thus far, it remains unknown whether the plastic chemicals or the particle itself are the driving factor for microplastic toxicity. To address this question, we exposed Daphnia magna for 21 days to irregular polyvinyl chloride (PVC), polyurethane (PUR) and polylactic acid (PLA) microplastics as well as to natural kaolin particles in high concentrations (10, 50, 100, 500 mg/L, ≤ 59 μm) and different exposure scenarios, including microplastics and microplastics without extractable chemicals as well as the extracted and migrating chemicals alone. All three microplastic types negatively affected the life-history of D. magna. However, this toxicity depended on the endpoint and the material. While PVC had the largest effect on reproduction, PLA reduced survival most effectively. The latter indicates that bio-based and biodegradable plastics can be as toxic as their conventional counterparts. The natural particle kaolin was less toxic than microplastics when comparing numerical concentrations. Importantly, the contribution of plastic chemicals to the toxicity was also plastic type-specific. While we can attribute effects of PVC to the chemicals used in the material, effects of PUR and PLA plastics were induced by the mere particle. Our study demonstrates that plastic chemicals can drive microplastic toxicity. This highlights the importance of considering the individual chemical composition of plastics when assessing their environmental risks. Our results suggest that less studied polymer types, like PVC and PUR, as well as bioplastics are of particular toxicological relevance and should get a higher priority in ecotoxicological studies.

Pesticides used in agriculture are some of the most common pollutants in the world. This study aimed to investigate the effects of Organophosphorus Pesticides (OPPs) and Organochlorine Pesticides (OCPs) on the families of farmworkers in the southeast of Iran.In the present case-control study, 141 family members of farmworkers (as the case group) and 59 family members of non-farmworkers (as the controls) were recruited. Serum levels of OCPs such as α-HCH, β-HCH, γ-HCH, 2,4-DDE, 4,4-DDE, 2,4-DDT, and 4,4-DDT were determined. In addition, erythrocyte acetylcholinesterase (AChE) activity, malondialdehyde (MDA), total antioxidant capacity (TAC), protein carbonyl (PC), nitric oxide (NO) serum levels, arylesterase activity of paraoxonase 1 (PON-1), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity were determined in all participants. Furthermore, distance to farmlands, education, crops, type, and the number of consumed fruits were evaluated for each individual separately.The erythrocyte AChE activity and serum activities of GPx, SOD, and PON-1 and TAC levels were significantly decreased, whereas the concentration of MDA, PC, NO, and seven OCPs were significantly increased in the farmworkers’ families as compared to the controls. Spearman correlation and linear regression suggest that OCPs increase the oxidative stress in farmworkers’ family members. Moreover, distance, education, farming precedence, products, and ventilation had significant effects on the OCP levels and increased the odds ratio of OCP levels in farmworkers’ families.With regards to the data obtained in this study, it was revealed that OCPs as illegal pesticides and OPPs were higher than expected in the farmworkers’ family members. Furthermore, exposure to OCPs and OPPs, apart from the other effects on the body, leads to oxidative stress (OS) that may cause serious diseases in the exposed populations.

In ecological risk assessment, acute to chronic ratio (ACR) uncertainty factors are routinely applied to acute mortality benchmarks to estimate chronic toxicity thresholds. To investigate variability of aquatic ACRs, we first compiled and compared 56 and 150 pairs of acute and subchronic/chronic growth/reproductive toxicity data for fishes (Pimephales promelas (53), Danio rerio (2), and Oryzias latipes (1)) and the crustacean Daphnia magna, respectively, for 172 chemicals with different modes of action (MOA). We found that there were only significant relationships between P. promelas acute median lethal concentrations and growth lowest-observed effect concentrations for class 1 (nonpolar narcosis) chemicals, though significant relationships were demonstrated for D. magna to all Verhaar et al. MOA classes (Class 1: nonpolar narcosis, Class 2: polar narcosis, Class 3: reactive chemicals, and Class 4: AChE inhibitors and estrogenics). Probabilistic ecological hazard assessment using chemical toxicity distributions was subsequently employed for each MOA class to estimate acute and chronic thresholds, respectively, to identify MOA and species specific ecological thresholds of toxicological concern. Finally, novel MOA and species specific ACRs using both chemical toxicity distribution comparison and individual ACR probability distribution approaches were identified using representative MOA and chemical categories. Our data-driven approaches and newly identified ACR values represent robust alternatives to application of default ACR values, and can also support future research and risk assessment and management activities for other chemical classes when toxicity information is limited for chemicals with specific MOAs within invertebrates and fish.

Iron nanoparticles (Fe NPs) have often been used for in situ remediation of both groundwater and soil. However, the impact of Fe NPs on the distribution and transformation of As species in contaminated soil is still largely unknown. In this study, green iron oxide nanoparticles synthesized using a euphorbia cochinchinensis leaf extract (GION) were used to stabilize As in a contaminated soil. GION exhibited excellent As stabilization effects, where As in non-specifically-bound and specifically-bound fractions decreased by 27.1% and 67.3% after 120 days incubation. While both arsenate (As (V)) and arsenite (As (III)) decreased after GION application, As (V) remained the dominant species in soil. X-ray photoelectron spectroscopy (XPS) confirmed that As (V) was the dominant species in specifically-bound fractions, while As (III) was the dominant species in amorphous and poorly-crystalline hydrous oxides of Fe and Al. Correlation analysis showed that while highly available As fractions were negatively correlated to oxalate and DCB extractable Fe, they were positively correlated to Fe²⁺ content, which indicated that Fe cycling was the main process influencing changes in As availability. X-ray fluorescence (XRF) spectroscopy also showed that the Fe₂O₃ content increased by 47.9% following GION soil treatments. Overall, this work indicated that As would be transformed to more stable fractions during the cycling of Fe following GION application and that the application of GION, even in small doses, provides a low-cost and ecofriendly method for the stabilization of As in soil.

The influence of palm oil biodiesel content on the cytotoxicity, mutagenicity and genotoxicity of particle- and gas-phase diesel vehicle emissions was investigated. The emissions were collected on-board of a EURO IV diesel truck, fuelled with mixtures of 10% (B10), 20% (B20) and 100% (B100) of palm oil biodiesel, under real driving conditions. Organic extracts of the particulate matter (PM) and gases were characterised for 17 PAH (including EPA priority) and used for the biological assay. Increasing biodiesel content in the fuel mixture results in a decrease in the PM and PAH emission factors, both in the particulate and gas-phase. The majority of the PAH are present in the gas-phase. The mutagenic potencies, in TA98 bacteria, are higher for B20 in both phases, whereas the mutagenicity emission factor, that takes into account the lower emission of PM and PAH, is not significantly different between the fuels. Higher direct mutagenicity (TA98 + S9) is observed in all the tested fuels, indicating the action of carcinogenic compounds other than non-substituted PAH. The gas-phase extracts present higher cytotoxicity and genotoxicity in lung epithelial cell A549, which may be related to the higher PAH content in the gas-phase. The increase in biodiesel content have a different impact on cytotoxicity, being larger in the gas-phase and lower in the particle-phase. This indicates that pulmonary toxicity may be higher for the gaseous emissions, due to the role of different toxic compounds compared to the PM. The adverse biological effects when biodiesel content increases are not consequent with the reduction of the PAH characterised, indicating that other toxic compounds are more relevant. Further investigations to identify these compounds are required in order to update and focus the efforts regarding emission targets and controls.

Phosphorus flame retardants (PFRs) have been widely detected in environmental media and human samples. Understanding the distribution and biotransformation of PFRs is important for toxicological research of PFRs in humans. C57BL/6 mice were administered with 300 mg/kg body weight/day of tris (1,3-dichloro-2-propyl) phosphate (TDCIPP) for 35 consecutive days. The liver, kidney, muscle, feces, urine and hair samples were collected to investigate the distribution of TDCIPP and its diester, bis (1,3-dichloro-2-propyl) phosphate (BDCIPP), as well as other potential metabolites of TDCIPP. Concentrations of TDCIPP in muscle (535.7 ± 192.2 ng/g wet weight) were significantly higher than those in liver (186.9 ± 55.0 ng/g wet weight) and kidney (43.5 ± 12.0 ng/g wet weight) (p < 0.05), while concentrations of BDCIPP were higher in kidney (2189.2 ± 420.7 ng/g wet weight) and liver (1337.1 ± 249.6 ng/g wet weight) than other tissues. The distribution of TDCIPP and BDCIPP in mice is tissue-specific, TDCIPP tends to accumulate in muscle, while BDCIPP tends to enrich in kidney and liver. BDCIPP was prone to be eliminated by urine, which may result in the high levels of BDCIPP in urine. Urine and feces had significantly higher concentrations of BDCIPP than TDCIPP (p < 0.05), which demonstrated that BDCIPP is an important metabolite of TDCIPP. Hair could serve as a suitable and reliable biomarker for TDCIPP and also metabolites of TDCIPP. Multifarious metabolites of TDCIPP were identified in various matrices of mice, especially urine. Seven novel metabolites of TDCIPP were identified for the first time. TDCIPP metabolic pathways involved oxidative dealkylation, oxidative dehalogenation, reoxidation, dehalogenation with dehydrogenation. The metabolites identified in the present study could serve as candidate biomarkers for future human biomonitoring studies.

Thifluzamide is widely used in treatment of rice diseases and has potential toxicity on aquatic organism. Although previous studies have focused on the toxic effect of thifluzamide in zebrafish, no consistent conclusions have been reached. To help to elucidate the toxic mechanism, qualities of liver and mitochondria were evaluated. The global changes in the transcriptome of zebrafish after exposure to thifluzamide were measured. Based on this, the expression and activities of chitinase and succinate dehydrogenase (SDH) were further assayed. And the targeted site of thifluzamide in zebrafish was confirmed by dock study and co-exposure study. Here we report that developmental inhibition was observed along with presence of liver and mitochondrial damage. The expression of SDHa-d and genes related to mitochondrial DNA (mtDNA) replicate and mitochondrial complexes were significantly altered. And, as the top differentially expressed genes, the expression of chia.1-6 did show apparent changes, but differences of chitinase activity between exposure groups and the controls did not reach significance. In line with that, dock study showed that the binding potentials of thifluzamide toward zebrafish chitinase and SDH exhibited in the following order: SDH> chitinase. And sdhb-sdhc-sdhd (Qp site) showed the highest binding activity toward thifluzamide. The joint exposure (thifluzamide + Q10) significantly improved the survival of zebrafish compared with single thifluzamide exposure. These results indicate that SDH, especially Qp-site, may be the target of thifluzamide in zebrafish and inhibition of SDH activity may be at least in partial responsible for the toxicity of thifluzamide in zebrafish. In addition, the antagonistic effect of Q10 on thifluzamide toxicity in zebrafish suggests that Q10 may be a useful adjunct to detoxification.