Attention deficit hyperactivity disorder (ADHD) is associated with heavy metal exposure during adolescent development. However, the direct clinical evidence is limited. To investigate the possible association between environmental heavy metal exposure and ADHD, a case-control study was conducted with children aged 6–14 years in Guangzhou, China. Results showed that median concentrations of chromium (Cr), manganese (Mn), cobalt (Co), nickel (Ni), copper (Cu), molybdenum (Mo), tin (Sn), barium (Ba), and lead (Pb) in the urine of the case group were significantly higher than those of the control group. Children with ADHD had significantly higher levels of 8-OHdG and MDA compared with those from the control group. In addition, correlations between urinary Co, Ni, Cu, Mo, and Sn were significantly correlated with 8-OHdG and MDA concentrations in urine. After the case and control groups were combined together and the first quartile was used as the reference category, odds ratios (ORs) of ADHD for children increased significantly with the quartile increasing of urinary Co, Cu, and Sn. Our study provides a clinical evidence that Co, Cu, and Sn exposure, particularly Sn exposure, may be an environmental risk of the incurrence of ADHD for children. Furthermore, Co, Ni, Cu, Mo, and Sn exposures were significantly correlated with DNA and lipid damage.

The fungicide carbendazim is an ecotoxic pollutant frequently found in water reservoirs. The ability of microorganisms to remove pollutants found in diverse environments, soil, water, or air is well documented. Although microbial communities have many advantages in bioremediation processes, in many cases, those with the desired capabilities may be slow-growing or have low pollutant degradation rates. In these cases, the manipulation of the microbial community through enrichment with specialized microbial strains showing high specific growth rates and high rates and efficiencies of pollutant degradation is desirable. In this work, bacteria of the genera Klebsiella, Flavobacterium, and Stenotrophomonas, isolated from the biofilm attached to the packed zones of a biofilm reactor, were able to grow individually in selective medium containing carbendazim. In the three bacteria studied, the mheI gene encoding the first enzyme involved in the degradation of the fungicide carbendazim was found. Studying the dynamics of growth and carbendazim degradation of the three bacteria, the effect of co-formulants was also evaluated. The pure compound and a commercial formulation of carbendazim were used as substrates. Finally, the study made it possible to define the biokinetic advantages of these strains for amendment of microbial communities.

The Chinese government, as a policy response, has continued to invest efforts and resources to implement cost-effective air pollution control technologies and stringent regulation to reduce emissions from the most contributing sectors to protect the environment and public health. The higher density of monitoring stations (> 1600) distributed across China provides a timely opportunity to use them to study in detail the national pollution trends in light of more stringent air pollution control policies. In the present study, air quality datasets comprising hourly concentrations of PM₂.₅, O₃, NO₂, and SO₂ collected across 1309, 1341, 1289, and 1347 monitoring stations respectively were obtained from the National Environmental Monitoring Centre over 4 years (2015–2018) and trend analysis was performed. Results indicate that the overall annual trends for PM₂.₅ and SO₂ were − 2.9 ± 2.7 and − 3.2 ± 3.2 μg/m³/year, while the winter trends were − 4.8 ± 5.8 and − 6.9 ± 8.4 μg/m³/year respectively across China. The daily maximum 8-h average (DMA8) ozone concentration showed a significant positive trend of 2.4 ± 4.6 μg/m³/year, which was comparatively higher in summer at 4.4 ± 9.0 μg/m³/year. On the other side, NO₂ trend is not great in number (− 0.45 ± 2.0 μg/m³/year). Overall, 62.2%, 61.8%, and 20.9% of PM₂.₅, SO₂, and NO₂ monitoring stations were associated with a negative trend of ≥ − 2 μg/m³/year. For O₃ DMA8 concentrations, 50.7% of the monitoring stations showed a significant positive trend of ≥ 2 μg/m³/year. In light of the Chinese government’s increasing impetus on combating air pollution and climate change via new policy regulations, it is important to understand the spatio-temporal distributions and relative contributions of the spectrum of gaseous pollutants to the pollution loads as well as identify changing emission loads across sectors. The results of this study will facilitate the formulation of evidence-based air pollution reduction strategies and policies.

The aim of the current research was to systematically review and summarize the studies that evaluated the concentration of lead (Pb) and cadmium (Cd) in cow milk in different regions of Iran and to perform a meta-analysis of the findings. Moreover, the non-carcinogenic and carcinogenic risks of Pb and Cd through milk consumption in adult and child consumers were assessed. As a result of a systematic search in the international and national databases between January 2008 and October 2018, 17 reports involving 1874 samples were incorporated in our study for meta-analysis. The pooled concentrations of Pb and Cd were estimated to be 13.95 μg mL⁻¹ (95% CI 9.72–18.11 μg mL⁻¹) and 3.55 μg mL⁻¹ (95% CI − 2.38–9.48 μg mL⁻¹), respectively, which were lower than the WHO/FAO and national standard limits. The estimated weekly intake (EWI) of Pb and Cd through consuming milk was 16.65 and 7 μg day⁻¹ for adults of 70 kg and 45 and 34 μg day⁻¹ for children of 26 kg, respectively, which was well below the risk values set by Joint FAO/WHO Expert Committee on Food Additives (JECFA). The maximum target hazard quotient values (THQs) of Pb and Cd were 5.55E−5 and 5.55E−5 for adults and 5.55E−5 and 5.55E−5 for children, respectively, which were lower than 1 value, suggesting that Iranian consumers are not exposed to non-carcinogenic risk through consuming milk. Moreover, the incremental lifetime cancer risk (ILCR) of Pb estimated to be 2.96E−04 in adults and 1.0E−03 in children, indicating that consumers in Iran are at threshold carcinogenic risk of Pb through consuming milk (ILCR > 10⁻⁴). Therefore, planning and policy making for the sustainable reduction of these toxic metals in milk, particularly in industrial regions of Iran, are crucial.

Areas contaminated with heavy metals can pose major risks to human health and ecological environments. The aims of this study are to assess human health risk and pollution index for heavy metals in agricultural soils irrigated by effluents of stabilization ponds in Birjand, Iran. The results revealed that the levels of Cr, Mn, Zn, Fe, Cu, Cd, and Pb were in range of 70.3–149.65, 355–570, 31.15–98.45, 23,925–29,140, 22.75–25.95, 0.17–6.51, and 8.5–23.5 mg/kg in topsoils, respectively. Total hazard index values from heavy metals through three exposure routes for adults and children were 9.13E−01 and 1.10, respectively, indicating that there was non-carcinogenic risk for children. The total risk of carcinogenic metals (Cr, Cd, and Pb) through the three exposure routes for adults and children was 1.06E−04 and 9.76E−04, respectively, which indicates that the metals in the soil will not induce carcinogenic risks to these age groups. Pollution levels of heavy metals in soil samples including enrichment factor (EF), contamination factor (CF), pollution load index (PLI), and geo-accumulation index (Igₑₒ) showed heavy metal contamination of agricultural soils. The results of the present study provide basic information about heavy metal contamination control and human health risk assessment management in the study area.

In the present study, the biochemical effect of nanocurcumin (nanoCUR) compared with Gliclazide (GLZ) on the diabetic rats was studied. Forty male albino rats (Sprague Dawley) weighted 110 ± 20 g were used. Rats were randomly separated into two groups. Control, received no treatment. Streptozotocin (STZ)-induced diabetic groups take 5 ml/kg of STZ in normal saline daily for 30 days, further divided into diabetic non-treated group, did not receive any treatment: diabetic group treated by nanoCUR, received 15 mg/kg/day of nanoCUR orally for 30 days; diabetic group treated by GLZ, received 2 mg/kg/day of GLZ for 30 days. The mean body weights of all rats were registered and serum samples were collected for determination of fasting blood glucose (FBG), insulin concentration, liver glucokinase (GK), and glycogen synthase (GS) activities. Liver tissues were collected for determination of mRNA expression of insulin (INS), insulin receptor A (IRA), glucokinase (GK), and glucose transporter 2 (GLUT2). The results revealed a significant reduction of body weight in diabetic rats, with no significant differences in nanoCUR and GLZ groups. There was a decline in FBG levels and significant elevation of INS levels, GK, and GS activities in diabetic rats received nanoCUR and GLZ. mRNA expression of INS, IRA, GK, and GLUT2 significantly upregulated in diabetic rats received nanoCUR and GLZ. The amazing observation was a non-significant difference in all measured parameters between nanoCUR and GLZ groups. In conclusion, nanoCUR is able to improve cellular uptake of glucose, the hepatic insulin signaling, and insulin sensitivity in diabetic rats. Its effect was similar to standard hypoglycemic drug (GLZ).

The objective of this study was to develop a process for the synthesis of magnetite nanocomposites from raffinate of hydrometallurgical plants containing iron ions. Towards this goal, a three-step process was developed. After the reduction of Fe(III) to Fe(II) in the first stage, Al³⁺ in the raffinate was precipitated as Al(OH)₃ and finally magnetite NPs were synthesized by chemical co-precipitation method via controlling the oxidation-reduction potentials (ORPs). The produced nanomaterials were analyzed using XRD, TEM, VSM, TGA, and FTIR. The TG, XPS, and FTIR results affirmed the existence of PVA while TEM images illustrated the spherical nanoparticles with an average size of about 19 ± 4 nm. Evaluation of VSM data indicated the highly ferromagnetic behavior for Fe₃O₄ and Fe₃O₄@PVA products at the room temperature with a saturation magnetization of 46.98 and 35.78 emu/g, respectively.

Hematological variances were seen in Ctenopharyngodon idella when exposed to (LC50 and LC85) copper, chromium, and lead concentration groups. The metal-exposed blood samples after collection were analyzed for comparative analysis of hematological alterations in the reference and treated groups at the different exposure hours. A significant reduction (p < 0.05) in hemoglobin, RBC, hematocrit, total leucocyte, and lymphocyte counts was observed with the increase in metal concentrations, whereas a significant incline (p < 0.05) in granulocyte and monocyte counts was observed with the administered doses of selected toxic metals as compared with the values obtained from the reference group. More pronounced changes in hematological indices both for the red and white blood cells were noticed at the highest dose of LC85-administered metal groups. The induced hematological changes were independent of the exposed duration pattern. The present investigation has significantly contributed in knowledge assessment of metal toxicity based on inclusive evaluation of dose-response relationships.

Unintentional injuries pose a great risk for human health in China. Few studies have focused on unintentional injuries at national level from urbanization perspective. The panel data of mortality rate of transportation accidents (TA), fall and drowning and sinking (DS) is investigated, and urbanization development index is collected. Global Moran’s I and linear regression of panel data are applied to determine the spatial distribution and spatial influencing factors of unintentional injuries. The results are the following: (1) The unintentional injury such as TA, fall, and DS shows clear non-uniformity of spatial distribution and relative immobility through time. (2) A 10,000 tons increase in SO₂ emission amount (SO₂ EA) and emission of smoke and dust (ESD) can result in 15.7 and 12.5 increases in TA death in eastern region, respectively. Meanwhile, A 10,000 tons increase in NOₓ emission amount can cause 15.1 increase in TA death in western region. For every 100 billion yuan increase in GDP, the fall death can reduce by 8.4 in central region. One bed increase in number of hospital beds per 10,000 population (NHBP) is favorable for decreasing in fall death by 16.7 in eastern region. However, increase in number of workers enjoying industrial injury (NEWII) does not reduce the fall death in eastern region. (3) For every 1 ten thousand people increase in number of students in ordinary high schools (NSOHS) is conductive to reducing DS death by 7.8 in the western region. Our findings show that there exist spatial differences for urbanization influencing TA, fall, and DS death in eastern, western, and central regions. This study is expected to provide a reference for unintentional injuries control in those three regions.

The present study was designed to see the influence of selenium (Se) and sulphur (S) in the alleviation of arsenic (As)-induced stress in Brassica juncea plant. Se-induced alterations in physiological and biochemical responses due to deficient S (DS), normal S (NS) and additional S (AS) conditions were evaluated in 14-day-old seedlings of B. juncea variety Varuna. During the last 7 days of the 14-day-old seedlings, supplementation with arsenite (Asᴵᴵᴵ, 300 μM) alone and its combination with selenite (Seᴵⱽ, 50 μM) along with different S treatments was done which are as follows: (i) control; (ii) As; (iii) As+Se+DS; (iv) As+Se + NS; (v) As+Se + AS. Experimental results showed that the application of AS in spite of NS supplied with Se influenced plant growth, oxidative stress and thiol-ascorbate-related parameters more prominently under As stress. The plants with As+Se+AS treatment exhibited lower ROS (superoxide and hydrogen peroxide ion), malondialdehyde (MDA) accumulation and lipoxygenase activity with increased activities of superoxide dismutase, catalase and ascorbate peroxidase compared with As+Se+NS condition. These plants also exhibited an increase in cysteine, non-protein thiols and phytochelatins, along with reduced, oxidised and redox content of glutathione and ascorbate. Furthermore, the application of S along with Se increased the activities of glutathione reductase, glutathione S-transferase, glutathione peroxidase, monodehydroascorbate and dehydroascorbate to minimise As stress. However, we observed that these responses were reversed under As+Se+DS condition and induced oxidative stress, which was almost similar to As only treatment. It indicated that AS nutrition potentiated Se to alleviate As-inhibited plant growth by modulating antioxidants including thiol-ascorbate-based mechanism and reducing As accumulation in B. juncea plants.