IMO’s Anti-Fouling Systems convention banned the use of organotin-based antifouling systems in 2008 as the ultimate effort to stop tributyltin (TBT) inputs into the marine environment. One of the hazardous effects of TBT is imposex (the superimposition of male sexual characters onto gastropod females), a phenomenon that may cause female sterility and the gastropod populations decline. Despite previous European Union legislation had already been shown effective in reducing the imposex levels along the Portuguese coast, this study intends to confirm these decreasing trends after 2008 and describe the global evolution in the last 15 years. Imposex levels were assessed in two bioindicators – the dog-whelk Nucella lapillus and the netted-whelk Nassarius reticulatus (Gastropoda, Prosobranchia) – in 2011 and 2014, and the results were compared with previous years. Both species showed progressive decreasing trends in imposex levels over the last 15 years; median values of the vas deferens sequence index (VDSI) fell from 3.96 to 0.78 in N. lapillus and from 3.39 to 0.29 in N. reticulatus. The temporal/spatial evolution of imposex suggests an apparent shift of TBT hotspots, being now restricted to fishing ports and marinas in detriment of large commercial harbours where TBT levels fell rapidly. Butyltins were measured in the whole tissues of N. lapillus females collected in 2014: monobutyltin (MBT) varied from < DL (detection limit: 1 ng Sn/g) to 13 ng Sn/g dw, dibutyltin (DBT) from 2.2 to 27 ng Sn/g dw and TBT from 1.5 to 55 ng Sn/g dw. Although TBT body burden has declined over time, the butyltin degradation index ([MBT]+[DBT])/[TBT] exhibited values < 1 in c. a. 90% of the sites assessed, suggesting that recent TBT inputs are still widespread in the Portuguese coast eventually due to illegal use of TBT antifouling systems and TBT desorption from sediments.

Fungi are well associated with the degradation of hydrocarbons by the production of different enzymes, among which catalases (CBH), laccases (LCC) and peroxidases (LiP and MnP) are of immense importance. In this study, crude oil tolerance and enzyme secretions were demonstrated by rhizospheric fungal strains. Four most abundant strains were isolated from the rhizosphere of grasses growing in aged oil spill sites and identified through morphological characterization and molecular PCR-amplification of 5.8–28S ribosomal rRNA using ITS1 and ITS4 primers. These strains were subjected to crude oil tolerance test at 0–20% concentrations. Presence and transcriptase responses of putative genes lig (1–6), mnp, cbh (1.1, 1.1 and 11), and lcc encoding lignin peroxidase, manganese peroxidase, catalase, and laccase enzymes respectively were also studied in these strains using RT-PCR. In addition, activities of secreted enzymes by each strain were studied in aliquots. The strains were identified as Aspergillus niger asemoA (KY473958), Talaromyces purpurogenus asemoF (KY488463), Trichoderma harzianum asemoJ (KY488466), and Aspergillus flavus asemoM (KY488467) through sequencing and comparing the sequences’ data at NCBI BLAST search software. All the isolated strains showed tolerance to crude oil at 20% concentration, but the growth rate reduced with increasing in oil concentrations. All the isolated strains possess the tested genes and lig 1–6 gene was overexpressed in A. niger and T. harzianum while lcc and mnp genes were moderately expressed in all the four strains. Almost 145 U.mL⁻¹ of lignin and manganese peroxidase, 87 U.mL⁻¹ of catalase, and 180 U.mL⁻¹ of laccase enzymes were produced by these strains and it was also observed that these strain mostly produced studied enzymes in response to increasing crude oil concentrations. Considering the robust nature and diverse production of these catalytic enzymes by these strains, they can be exploited for various bioremediation technologies as well as other biotechnological applications.

Air pollution has been shown to be significantly associated with morbidity and mortality in urban areas, but there is lack of studies focused on extreme pollution events such as extreme dust episodes in high-density Asian cities. However, such cities have had extreme climate episodes that could have adverse health implications for downwind areas. More importantly, few studies have comprehensively investigated the mortality risks of extreme dust events for socioeconomically vulnerable populations.This paper examined the association between air pollutants and mortality risk in Hong Kong from 2006 to 2010, with a case-crossover analysis, to determine the elevated risk after an extreme dust event in a high-density city. The results indicate that PM₁₀₋₂.₅ dominated the all-cause mortality effect at the lag 0 day (OR: 1.074 [1.051, 1.098]). This study also found that people who were aged ≥ 65, economically inactive, or non-married had higher risks of all-cause mortality and cardiorespiratory mortality during days with extreme dust events. In addition, people who were in areas with higher air pollution had significantly higher risks of all-cause mortality and cardiorespiratory mortality.In conclusion, the results of this study can be used to target the vulnerable among a population or an area and the day(s) at risk to assist in health protocol development and emergency planning, as well as to develop early warnings for the general public in order to mitigate potential mortality risk for vulnerable population groups caused by extreme dust events.

Increased generation of shrimp shell from exploitation of krill results in emerging biowaste pollution, in addition, uranium pollution has drawn public concern due to the rapid development of nuclear power, uranium mining, and nuclear fuel processing. In this study, krill shells were recovered and used as a potential natural biosorbent for uranium immobilization, thereby enabling both uranium decontamination and krill shell reutilization. Interaction of uranium with krill shell surface and their transformation were investigated by using batch sorption experiments, scanning electron microscopy, and transmission electron microscopy. Krill shell had high uranium sorption ability. Uranium was transformed into a nano-scale precipitate. The mapping of phosphorus and uranium was related to the nano-scale precipitate, indicating that sorption of uranium was dependent on phosphorus. Surface chemisorption between phosphate in krill shell and uranium as well as the formation of the nano-scale precipitate were interpreted as the mechanism of uranium immobilization. Thus, natural krill shell waste has potential for extensive use as a promising and cost-effective sorbent for uranium immobilization and krill shell reutilization.

During the last several decades, persistent organic pollutants and metals cause great concern for their toxicity in organisms as well as for their bioaccumulation and/or trophic transfer through the food chains in ecosystems. A large number of studies therefore have focused on the trophic levels of organisms to illustrate food web structure, as a critical component in the study of pollutant dynamics and biomagnification. The trends in biomagnification of pollutants in food webs indeed provide fundamental information about the properties and fates of pollutants in ecosystems. The trophic magnification supports the establishment of a reliable trophic structure, which can further aid the understanding of the transport and exposure routes of contaminants in accumulation and risk assessments. Recently, efforts to interpret the food web structure using carbon and nitrogen stable isotope ratios have contributed to better understanding of the fate of pollutants in the ecosystem. However, it is known that this isotope analysis of bulk ones has many weaknesses, particularly for uncertainties on the estimate of trophic levels and therefore of magnification factors for studied organisms, enough to support a regulatory interpretation. In this review, we collate studies that investigated biomagnification characteristics of pollutants in aquatic ecosystems, along with calculated trophic magnification factors. Moreover, we introduce a novel approach, compound-specific stable isotope analysis of nitrogen in amino acids, to establish reliable food web structures and accurate trophic levels for biomagnification studies. This method promises to provide sound results for interpreting the influence of the pollutant in organisms, along with their bioaccumulation and magnification characteristics, as well as that in ecosystem.

Soil algal bioassays have been limited by their inability to evaluate several toxic endpoints because it is difficult to collect pure soil algae growing on and beneath the soil surface. This study describes the extension of a previously developed paper-disc method for analyzing soil toxicity to algae. The method can be used in conjunction with flow cytometric analysis and facilitates the assessment of previously proposed toxicity endpoints, such as the growth zone, biomass, and photosynthetic activity. We assessed the applicability of this paper-disc soil method using the green algae Chlamydomonas reinhardtii and Pseudokirchneriella subcapitata exposed to nickel-contaminated soil; examined cell sizes, cell granularity, enzyme activity, and oxidative stress as new toxicity endpoints using flow cytometry; and identified morphological changes in green algae assayed. The results showed that, used in conjunction with flow cytometry, the extended paper-disc soil method is sufficiently sensitive to detect decreases in cell granularity in C. reinhardtii and esterase activity in P. subcapitata. The method also revealed decreases in growth zone, biomass, and electron transfer from the reaction center to the quinone pool. Collectively, the results of this study indicate that soil algal bioassays using nonspecific algae can be used to assess soil quality, to derive several toxicity endpoints for individual cells, and to evaluate previously established flow cytometric toxicity endpoints.

Energy crops are an important renewable source for energy production in future. To ensure high yields of crops, N fertilization is a common practice. However, knowledge on environmental impacts of bioenergy plantations, particularly in systems involving trees, and the effects of N fertilization is scarce. We studied the emission of volatile organic compounds (VOC), which negatively affect the environment by contributing to tropospheric ozone and aerosols formation, from Miscanthus and willow plantations. Particularly, we aimed at quantifying the effect of N fertilization on VOC emission. For this purpose, we determined plant traits, photosynthetic gas exchange and VOC emission rates of the two systems as affected by N fertilization (0 and 80 kg ha−1 yr−1). Additionally, we used a modelling approach to simulate (i) the annual VOC emission rates as well as (ii) the OH. reactivity resulting from individual VOC emitted. Total VOC emissions from Salix was 1.5- and 2.5-fold higher compared to Miscanthus in non-fertilized and fertilized plantations, respectively. Isoprene was the dominating VOC in Salix (80–130 μg g−1 DW h−1), whereas it was negligible in Miscanthus. We identified twenty-eight VOC compounds, which were released by Miscanthus with the green leaf volatile hexanal as well as dimethyl benzene, dihydrofuranone, phenol, and decanal as the dominant volatiles. The pattern of VOC released from this species clearly differed to the pattern emitted by Salix. OH. reactivity from VOC released by Salix was ca. 8-times higher than that of Miscanthus. N fertilization enhanced stand level VOC emissions, mainly by promoting the leaf area index and only marginally by enhancing the basal emission capacity of leaves. Considering the higher productivity of fertilized Miscanthus compared to Salix together with the considerably lower OH. reactivity per weight unit of biomass produced, qualified the C4-perennial grass Miscanthus as a superior source of future bioenergy production.

Rapid industrialization in China in recent decades has resulted in soil contamination in some areas, raising the concern about food safety. Consumption of rice represents a major exposure route for the toxic elements cadmium (Cd) and arsenic (As). We collected 160 polished rice from local markets in 20 provinces in China and determined total Cd and As concentrations and As speciation. Total Cd concentration ranged from below the detection limit to 0.77 mg kg−1, with 10% of the samples exceeding the Chinese limit (0.2 mg kg−1). Rice Cd concentration showed a distinct geographical pattern, increasing from low levels in the north to high levels in the south of China. Median daily Cd intake from rice varied from 0.01 μg kg−1 body weight in the north to 0.61 μg kg−1 body weight in the south of China, representing between 1% and 73% of the tolerable daily intake (TDI) recommended by FAO/WHO. The highest median Cd intake from rice was in Hunan province with 2 times TDI. Total As concentration ranged from 0.011 to 0.186 mg kg−1, with inorganic As (iAs) and dimethylarsinic acid (DMAs) on average accounting for 69% and 31%, respectively. All samples were below the Chinese limit for iAs in rice (0.2 mg kg−1). There was no clear geographical pattern in rice total As concentration, but rice produced in northeastern China contained higher percentages of DMAs and lower percentages of iAs. This study highlights a high risk of Cd exposure from rice consumption for the population of southern China and suggested strategies for reducing Cd accumulation in rice crop.

Plastic pollution in the environment is currently receiving worldwide attention. Improper dumping of disused or abandoned plastic wastes leads to contamination of the environment. In particular, the disposal of municipal wastewater effluent, sewage sludge landfill, and plastic mulch from agricultural activities is a serious issue and of major concern regarding soil pollution. Compared to plastic pollution in the marine and freshwater ecosystems, that in the soil ecosystem has been relatively neglected. In this study, we discussed plastic pollution in the soil environment and investigated research on the effects of plastic wastes, especially microplastics, on the soil ecosystem. We found that earthworms have been predominantly used as the test species in investigating the effects of soil plastic pollution on organisms. Therefore, further research investigating the effects of plastic on other species models (invertebrates, plants, microorganisms, and insects) are required to understand the effects of plastic pollution on the overall soil ecosystem. In addition, we suggest other perspectives for future studies on plastic pollution and soil ecotoxicity of plastics wastes, providing a direction for such research.

Spatial and seasonal variations in nitrate contamination are a globally concern. While numerous studies have used δ¹⁵N-NO₃ and δ¹⁸O-NO₃ to elucidate the dominant sources of nitrate in groundwater, this approach has significant limitations due to the overlap of nitrate isotopic ranges and the occurrence of nitrate isotopic fractionation. This study quantitatively assessed the spatial and seasonal variations in the proportional contributions of nitrate sources from different land uses in the Tarom watershed in North-West Iran. To achieve this aim, orthogonal projection of the hydrochemical and isotopic dataset of the principal component analysis (PCA) as well as correlation coefficient matrix (Corr-PCA) were evaluated to reduce the dimensionality of the inter-correlated dataset. Next, a nitrate isotopic biplot accompanied with a Bayesian isotope mixing model (SIAR) were applied to specify the spatial and seasonal trends in the proportional contribution of three dominant sources of nitrate (fertilizers, animal manure and residential waste) in the watershed. Finally, in order to provide a sensitive framework for nitrate source appointment and overcome the associated limitations of dual nitrate isotope application, the integration of boron isotope (δ¹¹B) and strontium isotopic ratio (⁸⁷Sr/⁸⁶Sr) was introduced. The results revealed that the mean contribution of residential sewage increased (17%–27.5%), while the mean contribution of fertilizers decreased (28.3%–19%), from late spring to early autumn. Also, fertilizer was the highest contributor (42.1% ± 3.2) during late spring, especially in regions with more than 75% agricultural land. Meanwhile, the mean contribution of sewage was highest in early autumn (32.1% ± 2.8) in the areas with more than 20% residential land. These results were confirmed by coupled application of δ¹¹B and ⁸⁷Sr/⁸⁶Sr. This study provides a useful insight for environmental managers to verify groundwater pollution contributors and to better apply remedial solutions.