Background, aim, and scope Catch decline of freshwater fish has been recorded in several countries. Among the possible causes, habitat change is discussed. This article focuses on potentially increased levels of fine sediments going to rivers and their effects on gravel-spawning brown trout. Indications of increased erosion rates are evident from land-use change in agriculture, changes in forest management practices, and from climate change. The latter induces an increase in air and river water temperatures, reduction in permafrost, changes in snow dynamics and an increase in heavy rain events. As a result, an increase in river sediment is likely. Suspended sediment may affect fish health and behaviour directly. Furthermore, sediment loads may clog gravel beds impeding fish such as brown trout from spawning and reducing recruitment rates. To assess the potential impact on fine sediments, knowledge of brown trout reproductive needs and the effects of sediment on brown trout health were evaluated. Approach We critically reviewed the literature and included results from ongoing studies to answer the following questions, focusing on recent decades and rivers in alpine countries. Have climate change and land-use change increased erosion and sediment loads in rivers? Do we have indications of an increase in riverbed clogging? Are there indications of direct or indirect effects on brown trout from increased suspended sediment concentrations in rivers or from an increase in riverbed clogging? Results Rising air temperatures have led to more intensive precipitation in winter months, earlier snow melt in spring, and rising snow lines and hence to increased erosion. Intensification of land use has supported erosion in lowland and pre-alpine areas in the second half of the twentieth century. In the Alps, however, reforestation of abandoned land at high altitudes might reduce the erosion risk while intensification on the lower, more easily accessible slopes increases erosion risk. Data from laboratory experiments show that suspended sediments affect the health and behaviour of fish when available in high amounts. Point measurements in large rivers indicate no common lethal threat and suspended sediment is rarely measured continuously in small rivers. However, effects on fish can be expected under environmentally relevant conditions. River bed clogging impairs the reproductive performance of gravel-spawning fish. Discussion Overall, higher erosion and increased levels of fine sediment going into rivers are expected in future. Additionally, sediment loads in rivers are suspected to have considerably impaired gravel bed structure and brown trout spawning is impeded. Timing of discharge is put forward and is now more likely to affect brown trout spawning than in previous decades. Conclusions Reports on riverbed clogging from changes in erosion and fine sediment deposition patterns, caused by climate change and land-use change are rare. This review identifies both a risk of increases in climate erosive forces and fine sediment loads in rivers of alpine countries. Increased river discharge and sediment loads in winter and early spring could be especially harmful for brown trout reproduction and development of young life stages. Recently published studies indicate a decline in trout reproduction from riverbed clogging in many rivers in lowlands and alpine regions. However, the multitude of factors in natural complex ecosystems makes it difficult to address a single causative factor. Recommendations and perspectives Further investigations into the consequences of climate change and land-use change on river systems are needed. Small rivers, of high importance for the recruitment of gravel-spawning fish, are often neglected. Studies on river bed clogging are rare and the few existing studies are not comparable. Thus, there is a strong need for the development of methods to assess sediment input and river bed clogging. As well, studies on the effects to fish from suspended sediments and consequences of gravel beds clogging under natural conditions are urgently needed.

Background, aim, and scope Dicofol is widely used as a pesticide in agriculture applications. Since dicofol is mainly synthesized from dichlorodiphenyltrichlorethane (DDT), it contains DDT as an impurity. The European Community has forced Prohibition Directive 79/117/EEC to reduce DDT in dicofol formulations. Specifically, DDT content in a dicofol formulation cannot exceed 0.1%. The goal of this project was to determine the DDT content in dicofol formulations used in Turkey. Materials and methods Samples of all the dicofol formulations in Turkey were collected to quantify DDT and DDT-related compounds. Four replicates were used for each sample. GC/MS/MS was used to analyze p,p' and o,p' isomers of DDT, DDD, and DDE. A HPLC was used to determine p,p'-Cl-DDT concentrations. Results The total DDT content of the formulated dicofol was found between 0.3% and 14.3%. The concentration of p,p'-DDE ranged from 167 to 1,042 mg kg⁻¹ in dicofol samples. p,p'-DDT concentrations were found to be 32 to 183 mg kg⁻¹. The o,p'-DDT level ranged from 2 to 34 mg kg⁻¹ in the dicofol formulations analyzed. Discussion It was estimated that 617.8 kg of DDT was released from dicofol. The main impurity was identified as p,p-Cl-DDT. Based on these results, dicofol serves as a continuing source of DDT contamination. Conclusions All DDT concentrations in dicofol samples analyzed were higher than the permitted 0.1% level of Prohibition Directive 79/117/EEC. The reduction of dicofol is critical since it serves as a continual source of DDT contamination. Recommendations and perspectives DDT has been found in soil, water, and air samples. Dicofol has been identified as a contributor to continued DDT contamination in soil and water. More studies are needed to ascertain the source of DDT in the air.

Background, aim, and scope Heavy metals such as lead are well known to cause harmful health effects. Especially children are particularly susceptible to increased levels of lead in their blood. It is also a fact that lead concentration is increasing in the environment due to increased anthropogenic activity. The risk of heavy metal contamination is pronounced in the environment adjacent to large industrial complexes. In a combined case study, the environmental pollution by heavy metals was related to children's health in the vicinity of an industrial area located 4 km south-east from Bucharest about 2 km east from the nearest town--Pantelimon. This site includes companies processing different, nonferrous solid wastes for recovery of heavy metals and producing different nonferrous alloys and lead batteries. In this paper, mainly the results of environmental sampling and analyses are summarized. Materials and methods Water, soil, and atmospheric deposition samples were collected from different locations within 3 km from the industrial area. For comparison, samples were also taken from Bucharest. Water samples were filtered (<0.45 μm), extracted by salpetric acid, and quantified by ICP-OES and ICP-MS. Soil samples were dried, sieved (<2 mm), extracted by aqua regia and analyzed by AAS. In order to quantify the atmospheric deposition, three kinds of permanently open collecting pots were used on nine different sites between August and November 2006. Results At most sampling locations, the heavy metal concentrations in soil decrease with increasing distance to the presumably major source of pollution. Highest heavy metal concentrations were found in 10-20 cm soil depths. There were also decreasing heavy metal concentrations for atmospheric deposition with increasing distance to the industrial site. In surface and groundwater samples, traces of zinc, copper and lead were detected. Discussion The heavy metal concentrations in soil were increased in the study area, mostly under legal action limits in low-concern areas (e.g., 1,000 mg Pb/kg dry soil), but often above action limits for high-concern areas (100 mg Pb/kg dry soil) such as populated areas. The soluble lead concentrations in water samples indicate a need for monitoring and assessing water quality in more detail. The results for atmospheric deposition showed increased dust precipitation and heavy metal loads in the study area compared to Bucharest. However, based on mass flow balance calculations, the actual atmospheric deposition of heavy metals must be much lower than it was in the past decades. Conclusions It was shown that highest lead values in water, soil and atmospheric deposition are rather to be found near the investigated industrial site than at the control sites in Bucharest. Our results correspond very well with results that show that children from Pantelimon have significantly increased lead concentrations in their blood compared to children in Bucharest. The increased lead contamination around the investigated industrial area is likely to have caused the increased exposure for children living in Pantelimon. Recommendations and perspectives In high-concern areas, such as found in populated areas, further measures have to be taken to avoid health risks for people living in these areas. The measures already taken to reduce emissions from the industrial site will help to avoid further increases in heavy metal concentrations. In areas with exceeded action limits, measures have to be taken as required by law. Detailed risk assessments could help to take necessary actions to protect public health in this area. The public should be informed about the potential hazards of eating plants grown in that area. Educational programs for schools, informing children about the contamination, should lead to a better understanding of environmental problems and a more sustainable behavior in the future.

Background, aim, and scope Decabromodiphenyl ether (DecaBDE) is used as an additive flame retardant in polymers. It has become a ubiquitous environmental contaminant, particularly abundant in abiotic media, such as sediments, air, and dust, and also present in wildlife and in humans. The main DecaBDE constituent, perbrominated diphenyl ether (BDE-209), is susceptible to transformations as observed in experimental work. This work is aimed at identifying and assessing the relative amounts of products formed after UV irradiation of BDE-209. Materials and methods BDE-209, dissolved in tetrahydrofuran (THF), methanol, or combinations of methanol/water, was exposed to UV light for 100 or 200 min. Samples were analyzed by gas chromatography/mass spectrometry (electron ionization) for polybrominated diphenyl ethers (PBDEs), dibenzofurans (PBDFs), methoxylated PBDEs, and phenolic PBDE products. Results The products formed were hexaBDEs to nonaBDEs, monoBDFs to pentaBDFs, and methoxylated tetraBDFs to pentaBDFs. The products found in the fraction containing halogenated phenols were assigned to be pentabromophenol, dihydroxytetrabromobenzene, dihydroxydibromodibenzofuran, dihydroxytribromodibenzofuran, and dihydroxytetrabromodibenzofuran. The PBDEs accounted for approximately 90% of the total amount of substances in each sample and the PBDFs for about 10%. Discussion BDE-209 is a source of PBDEs primarily present in OctaBDEs but also to some extent in PentaBDEs, both being commercial products now banned within the EU and in several states within the USA. It is notable that OH-PBDFs have not been identified or indicated in any of the photolysis studies performed to date. Formation of OH-PBDFs, however, may occur as pure radical reactions in the atmosphere. Conclusions Photolysis of decaBDE yields a wide span of products, from nonaBDEs to hydroxylated bromobenzenes. It is evident that irradiation of decaBDE in water and methanol yields OH-PBDFs and MeO-PBDFs, respectively. BDE-202 (2,2',3,3',5,5',6,6'-octabromodiphenyl ether) is identified as a marker of BDE-209 photolysis. Recommendations and perspectives BDE-209, the main constituent of DecaBDE, is primarily forming debrominated diphenyl ethers with higher persistence which are more bioaccumulative than the starting material when subjected to UV light. Hence, DecaBDE should be considered as a source of these PBDE congeners in the environment.

Background, aim and scope Mixed pollution with trace elements and organic industrial compounds is characteristic for many spill areas and dumping sites. The danger for the environment and human health from such sites is large, and sustainable remediation strategies are urgently needed. Phytoremediation seems to be a cheap and environmentally sound option for the removal of unwanted compounds, and the hyperaccumulation of trace elements and toxic metals is seemingly independent from the metabolism of organic xenobiotics. However, stress reactions, ROS formation and depletion of antioxidants will also cause alterations in xenobiotic detoxification. Here, we investigate the capability of plants to detoxify chlorophenols via glutathione conjugation in a mixed pollution situation. Materials and methods Typha latifolia and Phragmites australis plants for the present study were grown under greenhouse conditions in experimental ponds. A Picea abies L. suspension culture was grown in a growth chamber. Cadmium sulphate, sodium arsenate and lead chloride in concentrations from 10 to 500 µM were administered to plants. Enzymes of interest for the present study were: glutathione transferase (GST), glutathione reductase, ascorbate peroxidase and peroxidase. Measurements were performed according to published methods. GST spectrophotometric assays included the model substrates CDNB, DCNB, NBC, NBoC and the herbicide Fluorodifen. Results Heavy metals lead to visible stress symptoms in higher plants. Besides one long-term experiment of 72 days duration, the present study shows time and concentration-dependent plant alterations already after 24 and 72 h Cd incubation. P. abies spruce cell cultures react to CdSO₄ and Na₂HAsO₄ with an oxidative burst, similar to that observed after pathogen attack or elicitor treatment. Cd application resulted in a reduction in GSH and GSSG contents. When a heavy metal mixture containing Na₂HAsO₄, CdSO₄ and PbCl₂ was applied to cultures, both GSH and GSSG levels declined. Incubation with 80 µM arsenic alone doubled GSSG values. Based on these results, further experiments were performed in whole plants of cattail and reed, using cadmium in Phragmites and cadmium and arsenic in Typha as inducers of stress. In Phragmites australis, GST activities for CDNB and DCNB were significantly reduced after short-term Cd exposure (24 h). In the same samples, all antioxidant enzymes increased with rising heavy metal concentrations. Typha latifolia rhizome incubation with Cd and As leads to an increase in glutathione reductase and total peroxidase activity and to a decrease in ascorbate peroxidase activity. Measurements of the same enzymes in leaves of the same plants show increased GR activities, but no change in peroxidases. GST conjugation for CDNB was depressed in both cattail rhizomes and leaves treated with Cd. After As application increased, DCNB enzyme activities were detected. Discussion T. latifolia and P. australis are powerful species for phytoremediation because they penetrate a large volume of soil with their extensive root and rhizome systems. However, an effective remediation process will depend on active detoxifying enzymes, and also on the availability of conjugation partners, e.g. glutathione and its analogues. Species-specific differences seem to exist between the regulations of primary defence enzymes like SOD, catalase, peroxidases, whereas others prefer to induce the glutathione-dependent enzymes. As long as the pollutant mix encountered is simple and dominated by heavy metals, plant defence might be sufficient. When pollution plumes contain heavy metals and organic xenobiotics at the same time, this means that part of the detoxification capacity, at least of glutathione-conjugating reactions, is withdrawn from the heavy metal front to serve other purposes. In fact, glutathione S-transferases show strong reactions in stressed plants or in the presence of heavy metals. The spruce cell culture was a perfect model system to study short-term responses on heavy metal impact. Overall, and on the canopy level, this inhibitory effect might result in a lower detoxification capacity for organic pollutants and thus interfere with phytoremediation. Conclusions We present evidence that pollution with heavy metals will interfere with both the oxidative stress defence in plants, and with their ability to conjugate organic xenobiotics. Despite plant-species-dependent differences, the general reactions seem to include oxidative stress and an induction of antioxidative enzymes. Several processes seem to depend on direct binding of heavy metals to enzyme proteins, but effects on transcription are also observed. Induction of xenobiotic metabolism will be obtained at high heavy metal concentrations, when plant stress is elevated. Recommendations and perspectives Plants for phytoremediation of complex pollution mixtures have to be selected according to three major issues: uptake/accumulation capacity, antioxidative stress management, and detoxification/binding properties for both the trace elements and the organic xenobiotics. By way of this, it might be possible to speed up the desired remediation process and/or to obtain the desired end products. And, amongst the end products, emphasis should be laid on industrial building materials, biomass for insulation or biogas production, but not for feed and fodder. Each of these attempts would increase the chances for publicly accepted use of phytoremediation and help to cure the environment.

Background, aim, and scope Selenium is a trace metalloid of global environmental concern. The boundary among its essentiality, deficiency, and toxicity is narrow and mainly depends on the chemical forms and concentrations in which this element occurs. Different plant species--including Brassica juncea--have been shown to play a significant role in Se removal from soil as well as water bodies. Furthermore, the interactions between such plants, showing natural capabilities of metal uptake and their rhizospheric microbial communities, might be exploited to increase both Se scavenging and vegetable biomass production in order to improve the whole phytoextraction efficiency. The aim of the present study was to evaluate the capability of selenite removal of B. juncea grown in hydroponic conditions on artificially spiked effluents. To optimize phytoextraction efficiency, interactions between B. juncea and rhizobacteria were designedly elicited. Materials and methods Firstly, B. juncea was grown on water-filtering agriperlite beds in the presence of three different selenite concentrations, namely, 0.2, 1.0, and 2.0 mM. Plant growth was measured after 3 and 6 weeks of incubation in order to establish the selenite concentration at which the best plant biomass production could be obtained. Afterwards, water-filtering agriperlite beds were inoculated either with a selenium-acclimated microbial community deriving from the rhizosphere of B. juncea grown, erstwhile, in a selenite-amended soil or with axenic cultures of two bacterial strains, vicelike Bacillus mycoides SeITE01 and Stenotrophomonas maltophilia SeITE02, previously isolated and described for their high resistance to selenite. These latter were seeded separately or as a dual consortium. Selenite was amended at a final concentration of 1.0 mM. Total Se content in plant tissues (both shoots and roots), plant biomass production, and persistence of bioaugmented microbial inocula during the experimental time were monitored. Moreover, parameters such as bioconcentration factor (BF) and phytoextraction efficiency (PE) were determined at the end of the testing run to evaluate the effects of the different bioaugmentation strategies adopted on selenite phytoextraction efficiency of B. juncea. Results A general but significant increase in capacity to extract and transport selenium to the epigeous plant compartments was recorded in B. juncea grown in beds augmented with microbial inocula, except for the treatment with B. mycoides SeITE01 alone. Nevertheless, a severe decrease in vegetable biomass production was observed after all microbial treatments with the exception of the plants that had received only S. maltophilia SeITE02. Actually, an increase in selenium phytoextraction efficiency up to 65% was observed in B. juncea, when this bacterial strain was inoculated. Discussion Emendation of B. juncea grown in water-filtering beds with a Se(IV)-acclimated microbial community caused a higher Se uptake along with a reduction of plant biomass yield with respect to plants grown without addition of the same bacterial inoculum. The increase of selenium BF in shoots suggests that the Se(IV)-acclimated microbial community not only elicited the plant capacity to absorb selenite, but also did improve the capacity to transport the metalloid to the epigeous compartments. On the other hand, the reduction in plant biomass yield might be related exactly to this improved capability of B. juncea to accumulate selenium at concentrations that are actually toxic for plants. Differently, addition of two selenite-resistant bacterial strains, namely, S. maltophilia SeITE02 and B. mycoides SEITE01, had weaker effects on plant biomass production when compared to those recorded in the presence of the Se(IV)-adapted microbial community. In particular, inoculation of water-filtering beds with the SeITE02 strain alone was the sole strategy resulting in a positive effect on both plant biomass production in stressful conditions and the capacity of shoots to accumulate selenium. In fact, its putative ability of reducing Se(IV) to organo-Se compounds significantly enhanced either selenium absorption by the plants or active metalloid translocation to epigeous parts. Conclusions Bioaugmentation with the bacterial strain S. malthophila SeITE02 is suggested to elicit selenite phytoextraction efficiency in B. juncea. Recommendations Manipulation of synergistic interactions between plants having phytoextraction capabilities and their associated rhizobacteria may enhance already consolidated treatment processes aimed to detoxify selenite laden wastewater.

Background, aim, and scope The controversy over the world's first genetically modified (GM) wheat, Roundup Ready wheat (RRW), challenged the efficacy of 'science-based' risk assessment, largely because it excluded the public, particularly farmers, from meaningful input. Risk analysis, in contrast, is broader in orientation as it incorporates scientific data as well as socioeconomic, ethical, and legal concerns, and considers expert and lay input in decision-making. Local knowledge (LK) of farmers is experience-based and represents a rich and reliable source of information regarding the impacts associated with agricultural technology, thereby complementing the scientific data normally used in risk assessment. The overall goal of this study was to explore the role of farmer LK in the a priori risk analysis of RRW. Materials and methods In 2004, data were collected from farmers using mail surveys sent across the three prairie provinces (i.e., Manitoba, Saskatchewan, and Alberta) in western Canada. A stratified random sampling approach was used whereby four separate sampling districts were identified in regions where wheat was grown for each province. Rural post offices were randomly selected in each sampling district using Canada Post databases such that no one post office exceeded 80 farms and that each sampling district comprised 225-235 test farms (n = 11,040). In total, 1,814 people responded, representing an adjusted response rate for farmers of 33%. A subsequent telephone survey showed there was no non-response bias. Results The primary benefits associated with RRW were associated with weed control, whereas risks emphasized the importance of market harm, corporate control, agronomic problems, and the likelihood of contamination. Overall, risks were ranked much higher than benefits, and the great majority of farmers were highly critical of RRW commercialization. In total, 83.2% of respondents disagreed that RRW should have unconfined release into the environment. Risk was associated with distrust in government and corporations, previous experience with GM canola, and a strong belief in the importance of community and environment. Farmers were critical of expert-based risk assessment, particularly RRW field trials, and believed that their LK was valuable for assessing agbiotechnology as a whole. Discussion Over 90% of canola production across the Canadian prairies makes use of herbicide-tolerant (HT) varieties. Yet, respondents were generally uniform in their criticism of RRW, regardless whether they were HT users, non-HT-users, conservation tillage or organic in approach. They had a sophisticated understanding of how GM trait confinement was intrinsically tied to grain system segregation and, ultimately, market accessibility, and were concerned that gene flow in RRW would not be contained. Organic farmers were particularly critical of RRW, in large part because certification standards prohibit the presence of GM traits. Farmers practicing conservation tillage were also at relatively great risk, in part because their dependence on glyphosate to control weeds increases the likelihood that RRW volunteer would become more difficult and costly to control. Conclusions This research is the first of its kind to include farmer knowledge in the a priori risk analysis of GM crops and, arguably, given its prairie-wide scope, is the largest scale, independent-farmer-focused study on GM crops ever conducted. The surprising uniformity in attitudes between users and non-users of GM technology and among organic, conventional, conservation tillage and GM using farmers speaks to the ability of farmers to discriminate among HT varieties. Our results clearly show that prairie farmers recognize that the risks associated with RRW commercialization outweigh any benefits. Recommendations and perspectives Farmer knowledge systems are holistic in nature, incorporating socioeconomic, cultural, political, and agroecological factors that all can contribute meaningfully to the pre-release evaluation of GM crops. The inclusion of farmers and other stakeholders in risk assessment will also help enhance and even restore public confidence in science-focused approaches to risk assessment. Although farmers are highly knowledgeable regarding RRW and arguably any agricultural technology, their expertise continues to be overlooked by decision-makers and regulators across North America.

Background, aim, and scope Benzotriazoles (BT) as 1H-benzotriazole (1H-BT), 5-methyl-1H-benzotriazole (5Me-BT), and 4-methyl-1H-benzotriazole (4Me-BT) are frequently used as corrosion inhibitors in dish washer detergents, aircraft de-icing/anti-icing fluids (ADAF), automotive antifreeze formulations, brake fluids, fluids for industrial cooling systems, metal-cutting fluids, and in solid cooling lubricants. Discharge of treated municipal waste water and controlled over-runs of combined waste water sewers are potential point sources for BT in rivers. The aim of this monitoring study was to yield an overview on exposure concentrations and loads of BT in the German rivers Main, Hengstbach, and Hegbach. Materials and methods Concentrations of 1H-BT, 5Me-BT, and 4Me-BT were determined in grab samples collected from different sampling points in the rivers Main, Hengstbach, and Hegbach at four different sampling times. Main and Hengstbach rivers were sampled close to Frankfurt International Airport. Both rivers receive domestic waste water effluents. BT were extracted from 2.5 L of river water by solid phase extraction using Bond Elut ppl cartridges (200 mg/3 mL). The extracts were analyzed by gas chromatography/mass spectrometry in full scan mode. Mass flows of BT were calculated by concentrations multiplied by mean daily river flow rates. Median concentrations and mass flows were compared for different rivers. Mass flows were also compared for selected sampling points at different sampling times. Results 1H-BT, 5Me-BT, and 4Me-BT were detected in Main and Hengstbach rivers. 1H-BT and 5Me-BT were also detected in Hegbach River. Concentrations ranged from 38 to 1,474 ng/L for 1H-BT, from 25 to 281 ng/L for 5Me-BT, and from 25 to 952 ng/L for 4Me-BT. Median concentrations of 1H-BT, 5Me-BT, and 4Me-BT were lower in Main than in Hengstbach River. Much higher median mass flows of all BT were calculated for Main than for Hengstbach River. At sampling points P9 (Main) and P5 (Hengstbach) concentrations of 4Me-BT and 5Me-BT increased from March 29, 2008 to May 1, 2008 to June 22, 2008 whereas daily mean river flow rate decreased simultaneously. However, concentration of 1H-BT in Main and Hengstbach River increased from March 29, 2008 to May 1, 2008 and decreased again on June 22, 2008. In the Main River, lowest and highest mass flows for all BT were calculated on June 22, 2008 and May 1, 2008, respectively. In the Hengstbach River lowest and highest mass flows for 1H-BT and 4Me-BT were also calculated on June 22, 2008 and May 1, 2008, respectively. However, mass flows of 5Me-BT in Hengstbach River were rather similar at all three sampling times. In all grab samples, 1H-BT was more abundant than 5Me-BT and 4Me-BT in Main and Hengstbach River, except on June 22, 2008. Ratios of 1H-BT/(5Me-BT + 4Me-BT) determined on March 15, 2008, March 29, 2008, and May 1, 2008 varied between 1.6 and 9.0 with a median value of 1.9 (n = 9) whereas on June 22, 2008 the ratios varied between 0.4 and 0.7 with a median value of 0.6 (n = 5). Discussion Due to the absence of waste water effluents in the Hegbach River, other input sources as controlled over-runs of combined waste water sewers and/or atmospheric deposition of BT must be regarded as possible input sources. Exfiltration of ground water containing BT to Hegbach River must be also regarded, especially when considering the high polarity of BT. Median concentrations of BT in Main River were much lower than in Hengstbach River due to dilution. However, median mass flows were higher in the Main River than in the Hengstbach River. Higher mass flows could be attributed to higher source strengths and/or numerous emissions sources in the Main River. Mass flows determined on June 22, 2008 in Main and Hengstbach rivers probably reflect emissions of BT only from dishwasher detergents since de-icing operations were unlikely at that time. Emissions of BT from dish washer detergents are rather constant without any seasonal variations. Assuming the absence of additional input sources and constant in-stream removal processes, mass flows calculated for all other sampling times must be nearly similar to mass flows for June 22, 2009 as it was only observed for 5Me-BT in Hengstbach River. The higher mass flows for 1H-BT and 4Me-BT in March and May in both rivers could be an indication for temporal variations of emission pattern and/or of in-stream removal processes. 1H-BT/(4Me-BT + 5Me-BT) ratios above one in March and May and below one in June could be also an indication for temporal variations of input and/or removal processes. Conclusions 1H-BT, 5Me-BT, and 4Me-BT used as corrosion inhibitors in many applications were detected in the rivers Main, Hengstbach, and Hegbach with relative high temporal and spatial concentration variations. Dilution is a dominant factor that influences exposure concentrations of BT in the studied rivers. We conclude that, especially in smaller rivers (as Hengstbach River), the hydrological situation has to be regarded when predicting exposure concentrations of BT. Characteristic emission strength and in-stream removal processes must be known to relate loads of BT in river water to different sources. The ratio of 1H-BT/(4Me-BT + 5Me-BT) could be possibly used for source apportionment. Recommendations and perspectives Time series analyses of BT in composite river water samples collected at two river sites of the Hengstbach/Schwarzbach catchment area, without any waste water effluents in between, are recommended to study in-stream removal of BT. In addition, exposure modeling is recommended of BT, regarding all input sources and in-stream removal processes to predict exposure concentrations of BT in rivers. In order to calibrate and validate the model, additional monitoring data are required.

Background, aim, and scope Foraging patches can be described as a nested hierarchy of aggregated resources, implying that study of foraging by wild animals should be directed across different spatial scales. However, almost all previous research on habitat selection by the giant panda has concentrated upon one scale. In this research, we carried out a field study to understand foraging patch selection by giant pandas in winter at both microhabitat and feeding site scales and, for the first time, attempted to understand how long it would stay at the feeding sites before moving on. Materials and methods The field survey was conducted from November 2002 to March 2003 at Fengtongzhai Nature Reserve (102°48'-103°00' E, 30°19'-30°47' N), Baoxing County of Sichuan Province, China, to collect data in both microhabitat and control plots. The microhabitat plots were located by fresh feces or foraging traces left by giant pandas, and the control plots were established to reflect the environment. Within each microhabitat plot, one 1 x 1 m² plot was centralized at the center of each feeding site, in which numbers of old bamboos and old shoots, including eaten and uneaten, were counted, respectively. Results The results showed that winter microhabitats selected by this species were characteristic of gentle slopes and high old-shoot proportions and that the latter was even higher at feeding sites. Two selection processes, namely, from the environment to microhabitats and from the latter to feeding sites, were found during this species' foraging patch utilization. Giant pandas preferred to eat old shoots to old bamboo at feeding sites in winter and did not leave unless old-shoot density fell to lower than the average in the environment. Discussion Both microhabitats and feeding sites selected by giant pandas were characteristic of high old-shoot density, indicating that the preferred food item had a significant influence upon its foraging patch selection. The preference for gentle slopes by giant pandas was presumed to save energy in movement or reflect the need to sit and free its fore-limbs to grasp bamboo culms when feeding but also seemed to be correlated with an easier access to old shoots. The utilization of old shoots at feeding sites was assumed to help maximize energy or nutrient intake during their foraging. Conclusions The difference between microhabitat plots and control plots and between microhabitats and feeding sites uncovered a continuous selection process from the environment via microhabitats to feeding sites. The utilization of old shoots at feeding sites was parallel to the marginal value theorem. The selection and abandonment of foraging patches by giant pandas was an optimal behavioral strategy adapted to their peculiar food with high cellulose and low protein. Recommendations and perspectives Our results uncovered the importance of multiple scales in habitat selection research. To further understand the process of habitat selection, future research should pay more attention to resolve the question of how to locate foraging patches under dense bamboo forests by the giant panda, which was traditionally considered to have poor eyesight, although our research has answered what type of habitats the giant panda prefers and when to leave.

Background, aim and scope Chlorine is an abundant element, commonly occurring in nature either as chloride ions or as chlorinated organic compounds (OCls). Chlorinated organic substances were long considered purely anthropogenic products; however, they are, in addition, a commonly occurring and important part of natural ecosystems. Formation of OCls may affect the degradation of soil organic matter (SOM) and thus the carbon cycle with implications for the ability of forest soils to sequester carbon, whilst the occurrence of potentially toxic OCls in groundwater aquifers is of concern with regard to water quality. It is thus important to understand the biogeochemical cycle of chlorine, both inorganic and organic, to get information about the relevant processes in the forest ecosystem and the effects on these from human activities, including forestry practices. A survey is given of processes in the soil of temperate and boreal forests, predominantly in Europe, including the participation of chlorine, and gaps in knowledge and the need for further work are discussed. Results Chlorine is present as chloride ion and/or OCls in all compartments of temperate and boreal forest ecosystems. It contributes to the degradation of SOM, thus also affecting carbon sequestration in the forest soil. The most important source of chloride to coastal forest ecosystems is sea salt deposition, and volcanoes and coal burning can also be important sources. Locally, de-icing salt can be an important chloride input near major roads. In addition, anthropogenic sources of OCls are manifold. However, results also indicate the formation of chlorinated organics by microorganisms as an important source, together with natural abiotic formation. In fact, the soil pool of OCls seems to be a result of the balance between chlorination and degradation processes. Ecologically, organochlorines may function as antibiotics, signal substances and energy equivalents, in descending order of significance. Forest management practices can affect the chlorine cycle, although little is at present known about how. Discussion The present data on the apparently considerable size of the pool of OCls indicate its importance for the functioning of the forest soil system and its stability, but factors controlling their formation, degradation and transport are not clearly understood. It would be useful to estimate the significance and rates of key processes to be able to judge the importance of OCls in SOM and litter degradation. Effects of forest management processes affecting SOM and chloride deposition are likely to affect OCls as well. Further standardisation and harmonisation of sampling and analytical procedures is necessary. Conclusions and perspectives More work is necessary in order to understand and, if necessary, develop strategies for mitigating the environmental impact of OCls in temperate and boreal forest soils. This includes both intensified research, especially to understand the key processes of formation and degradation of chlorinated compounds, and monitoring of the substances in question in forest ecosystems. It is also important to understand the effect of various forest management techniques on OCls, as management can be used to produce desired effects.