Plastics are globally distributed in oceans and can pose a threat to the environment and organisms. In this study, plastic pollution in surface water and sediments of the Bohai Sea was assessed based on plastic abundance, distribution and characteristics (shape, polymer, size and color). Water and sediment samples were collected across the sea using a plankton net (330 μm) and a grab sampler, respectively. The following conclusions were reached. 1) In surface water, large plastics were less abundant (0.14 items/m³) and showed less diverse characteristics than microplastics (0.79 items/m³) but did not significantly differ in spatial distribution. 2) Microplastics in water were more abundant (1.95 items/m³) with more diverse characteristics in Liaodong Bay than in other regions of the sea (0.26–0.59 items/m³). Plastic waste from highly concentrated agricultural, industrial and fishery activities could make large contributions to microplastics in Liaodong Bay. Additionally, low hydrodynamics and long distance to Bohai Strait are unfavorable for diffusion of particles, facilitating the retention of microplastics and increasing the abundance in this bay. 3) Microplastics in sediments were smaller in terms of dominant sizes (<0.5 mm) with less diverse characteristics than particles in water (0.5–1.5 mm). Specifically, fragments, foams and lines dominated among the microplastics in water, whereas fibers and fragments were dominant particles in sediments; alkyd resin, polyethylene, polystyrene and polypropylene (PP) predominated among the particles in water, but rayon, cellulose and PP were dominant particles in sediments. 4) Neither abundance nor size of microplastics in the two media was proportionally correlated and showed low similarity indexes of polymer (0.16), shape (0.29) or color (0.38). This could be related to mismatch in spatiotemporal distributions and variations in the characteristics, fate and behavior of microplastics in the two media. The findings provide knowledge for tracing the sources of plastics in the Bohai Sea.

The mediating influence of thyroid function on the association of phthalate exposure with glucose metabolism, including insulin resistance, remains unclear. We explored the mediating influence of thyroid hormone levels on the phthalate exposure–insulin resistance association. This cross-sectional study of 217 Taiwanese adults assessed insulin resistance (Homeostatic Model Assessment for Insulin Resistance, HOMA-IR scores) and the levels of 11 urinary phthalate metabolites and 5 thyroid hormones. Multiple regression models were used to analyze the associations among serum thyroid hormone levels, urinary phthalate metabolite levels, and HOMA-IR scores. The mediation analysis assessed the influence of thyroid function on the phthalate exposure–HOMA-IR association. Our data indicated urinary mono-ethylhexyl phthalate (MEHP) levels was negatively associated with free thyroxine (T₄) (β = −0.018; 95% confidence interval [CI]: −0.031, −0.005) and positively associated with HOMA-IR scores (β = 0.051, 95% CI: 0.012, 0.090). The study also revealed urinary mono-(2-ethyl-5-oxohexyl) phthalate (MEOHP) levels was negatively associated with free T₄ (β = −0.036, 95% CI: −0.056, −0.017) and HOMA-IR (β = 0.070, 95% CI: 0.013, 0.126). Free T₄ and HOMA-IR had a negative association (β = −0.757, 95% CI: −1.122, −0.392). In the mediation analysis, free T₄ mediated 24% and 35% of the associations of urinary MEHP and MEOHP with HOMA-IR, respectively. Our findings revealed the mediating role of thyroid function in the phthalate exposure–glucose metabolism association in adults.

Insect gut microbiotas have a variety of physiological functions for host growth, development, and immunity. Bacillus thuringiensis (Bt) is known to kill insect pests by releasing insecticidal protoxins, which are activated in the insect midgut. However, the interplay among Bt infection, host immunity, and gut microbiota are still unclear. Here we show that Bt Cry1Ac protoxin interacts with the gut microbiota to accelerate the mortality of P. xylostella larvae. Cry1Ac protoxin was found to cause a dynamic change in the midgut and hemocoel microbiota of P. xylostella, with a significant increase in bacterial load and a significant reduction in bacterial diversity. In turn, loss of gut microbiota significantly decreased the Bt susceptibility of P. xylostella larvae. The introduction of three gut bacterial isolates Enterococcus mundtii (PxG1), Carnobacterium maltaromaticum (PxCG2), and Acinetobacter guillouiae (PxCG3) restored sensitivity to Bt Cry1Ac protoxin. We also found that Cry1Ac protoxin and native gut microbiota can trigger host midgut immune response, which involves the up-regulation of expression of Toll and IMD pathway genes and most antimicrobial peptide genes, respectively. Our findings further shed light on the interplay between insect gut microbiota and host immunity under the Bt toxin killing pressure, and this may provide insights for improving the management of Bt resistance and lead to new strategies for biological control of insect pests.

Oxygen vacancy-enriched N/P co-doped cobalt ferrite (NPCFO) was synthesized using ionic liquid as N and P sources, and then the catalytic performance and mechanism of NPCFO upon peroxymonosulfate (PMS) activation for the degradation of organic pollutants were investigated. The as-synthesized NPCFO-700 exhibited excellent catalytic performance in activating PMS, and the degradation rate constant of 4-chlorophenol (4-CP) increased with the increase of OV concentration in NPCFO-x. EPR analysis confirmed the existence of ·OH, SO₄·⁻, and ¹O₂ in the NPCFO-700/PMS system, in which OV could induce the generation of ¹O₂ by PMS adsorption and successive capture, and also served as electronic transfer medium to accelerate the redox cycle of M²⁺/M³⁺ (M denotes Co or Fe) for the generation of radical to synergistically degrade organic pollutants. In addition, the contribution of free radical and nonradical to 4-CP degradation was observed to be strongly dependent on solution pH, and SO₄·⁻ was the major ROS in 4-CP degradation under acid and alkaline condition, while ¹O₂ was involved in the degradation of 4-CP under neutral condition due its selective oxidation capacity, as evidenced by the fact that such organic pollutants with ionization potential (IP) below 9.0 eV were more easily attacked by ¹O₂. The present study provided a novel insight into the development of transition metal-based heterogeneous catalyst containing massive OV for high-efficient PMS activation and degradation of organic pollutants.

Epidemiological studies have reported association of urinary 3-phenoxybenzoic acid (3-PBA), a major metabolite of pyrethroid insecticides (PYRs), with respiratory disease. However, knowledge regarding its effect on pulmonary function in susceptible children is limited. This study aimed to assess the associations between environmental 3-PBA concentrations and pulmonary function in children aged 6–17 years. Using data on 1174 children aged 6–17 years from the U.S. National Health and Nutrition Examination Survey (NHANES) 2007–2012, the exposure to PYRs was assessed by measuring urinary 3-PBA concentrations and pulmonary function was assessed by spirometry. Multivariable linear regression and generalized linear models (GLMs) were used to examine the associations between 3-PBA concentrations and pulmonary function in children, controlling for confounders. We found that 3-PBA concentrations were inversely associated with forced expiratory volume in 1 s (FEV₁), forced vital capacity (FVC), and peak expiratory flow (PEF) in the pediatric population (p-trends < 0.05). When stratified by age (6–10 and 11–17 years) and gender (boys and girls), the adverse effects of PYR exposures on pulmonary function were more pronounced among boys aged 11–17 years. Among this age group, 3-PBA concentrations were negatively associated with FEV₁, FVC, forced expiratory flow between 25% and 75% of FVC (FEF₂₅–₇₅%), and PEF. However, among children aged 6–10 years, no associations were found between 3-PBA concentrations and any of the pulmonary function measures, in either boys or girls. Our findings suggest that environmental PYR exposures may adversely affect children’s pulmonary function, with the strongest associations among 11–17 years old boys.

Current study was carried out with an objective to remediate highly contaminated sludge with HMX and RDX obtained from an explosive manufacturing facility in North India employing indigenous microbes, Arthrobacter subterraneus (isolate no. S2-TSB-17) and Bacillus sonorensis (isolate no. S8-TSB-4) which were isolated from the same contaminated site. In-vessel composting of the explosive contaminated sludge was performed in 12 different bioreactors using cow manure and garden waste as bulking agents. 78.5% degradation of HMX was observed in reactor no. 2 with Bacillus sonorensis having combination of 10% sludge, 70% cow manure and 20% garden waste on 80th day. Two secondary metabolites Bis(hydroxymethyl)nitramine and methylene dinitramine were identified while studying the degradation pathway. Similarly, degradation of 91.2% was observed for RDX in reactor no. 11 with consortia of Arthrobacter subterraneus and Bacillus sonorensis on 80th day. During the study, release of significant nitrate and nitrite ions were observed. It has already been established that RDX and HMX degradation leads to release of nitrite/nitrate ions. The highest nitrite (reactor no. 11) and nitrate (reactor no. 2) release observed were 24.02 ± 0.05 mg/kg and 30.65 ± 0.99 mg/kg on 50th and 70th day, respectively. Scanning electron microscopic studies confirmed the attachment and presence of microbes with solid surface and no deformation in structure was observed in the microbial cells due to contamination stress. Findings of the study concluded that in-vessel composting assisted with native bacterial species can be a potential technology for the treatment of explosive contaminated sludge at the contaminated sites.

Coastal wetland soils serve as a great C sink or source, which highly depends on soil carbon flux affected by complex hydrology in relation to salinity. We conducted a field experiment to investigate soil respiration of three coastal wetlands with different land covers (BL: bare land; SS: Suaeda salsa; PL: Phragmites australis) from May to October in 2012 and 2013 under three groundwater tables (deeper, medium, and shallower water tables) in the Yellow River Delta of China, and to characterize the spatial and temporal changes and the primary environmental drivers of soil respiration in coastal wetlands. Our results showed that the elevated groundwater table decreased soil CO₂ emissions, and the soil respiration rates at each groundwater table exhibited seasonal and diurnal dynamics, where significant differences were observed among coastal wetlands with different groundwater tables (p < 0.05), with the average CO₂ emission of 146.52 ± 13.66 μmol m⁻²s⁻¹ for deeper water table wetlands, 105.09 ± 13.48 μmol m⁻²s⁻¹ for medium water table wetlands and 54.32 ± 10.02 μmol m⁻²s⁻¹ for shallower water table wetlands. Compared with bare land and Suaeda salsa wetlands, higher soil respiration was observed in Phragmites australis wetlands. Generally, soil respiration was greatly affected by salinity and soil water content. There were significant correlations between groundwater tables, electrical conductivity and soil respiration (p < 0.05), indicating that soil respiration in coastal wetlands was limited by electrical conductivity and groundwater tables and soil C sink might be improved by regulating water and salt conditions. We have also observed that soil respiration and temperature showed an exponential relationship on a seasonal scale. Taking into consideration the changes in groundwater tables and salinity that might be caused by sea level rise in the context of global warming, we emphasize the importance of groundwater level and salinity in the carbon cycle process of estuarine wetlands in the future.

Researches have shown that silica nanoparticles (SiNPs) could reduce both the quantity and quality of sperm. However, the mechanism of toxicity induced by SiNPs in the male reproductive system is still unclear. In this study, male mice were randomly divided into a control group, and SiNPs treated group (20 mg/kg dose; n = 30 per group). Half of the mice per group were sacrificed on 35 days and the remaining on 50 days of the SiNPs exposure. SiNPs were found to decrease sperm count and mobility, increase the sperm abnormality rate, and damage the testes' structure. Furthermore, SiNPs decreased the protein levels of Protamine 1(PRM1) and elevated the histones' levels and suppressed the chromatin condensation of sperm. There was a significant reduction of the ubiquitinated H2A (ubH2A)/H2B (ubH2B) and RING finger protein 8 (RNF8) levels in the spermatid nucleus, while the RNF8 level in the spermatid cytoplasm increased evidently. The protein expression levels of PIWI-like protein 1(MIWI) in the late spermatids significantly increased on day 35 of SiNPs exposure. After 15 days of the withdrawal, the sperm parameters and protamine levels, and histones in the epididymal sperm were unrecovered; however, the changes in testis induced by SiNPs were recovered. Our results suggested that SiNPs could decrease the RNF8 level in the nucleus of spermatid either by upregulating of the expression of MIWI or by inhibiting its degradation. This resulted in the detention of RNF8 in the cytoplasm that maybe inhibited the RNF8-mediated ubiquitination of ubH2A and ubH2B. These events culminated in creating obstacles during the H2A and H2B removal and chromatin condensation, thereby suppressing the differentiation of round spermatids and chromatin remodeling, which compromised the sperm quality and quantity.

Although secondary minerals have great potential for heavy metal removal, their impact on chromium biogeochemistry in subsurface environments associated with dissimilatory iron reducing bacteria (DIRB) remains poorly characterized. Here, we have investigated the mechanisms of biogenic secondary minerals on the rate of Cr(VI) bioreduction with shewanella oneidensis MR-1. Batch results showed that the biogenic secondary minerals, schwertmannite and jarosite, appreciably increased the Cr(VI) bioreduction rate. UV–vis diffuse reflection spectra showed that schwertmannite and jarosite are semiconductive minerals, which can be activated by MR-1, followed by transferred conduction electrons toward Cr(VI). Cyclic voltammetry and Tafel analysis suggested that the resistance of secondary minerals is a dominant factor controlling Cr(VI) bioreduction. In addition, Cr(VI) adsorption on secondary minerals through ligand exchange promoted Cr(VI) bioreduction by decreasing the electron transfer distance between MR-1 and chromate. Fe(III)/Fe(II) cycling in schwertmannite and jarosite also contributed to Cr(VI) bioreduction as reflected by X-ray photoelectron spectroscopy and Fourier transform infrared spectrometer. Complementary characterizations further verified the contributions of Fe(III)/Fe(II) cycling, Cr(VI) adsorption, and conduction band electron transfer to enhanced Cr(VI) bioreduction. This study provides new insights on the understanding of Cr(VI) bioreduction by semiconductor minerals containing sulfate in subsurface environments.

There is growing evidence of widespread contamination of freshwater ecosystems with microplastics. However, the effects of chronic microplastic ingestion and its interaction with other pollutants and stress factors on the life-history traits and the host-microbiome of aquatic invertebrates are not well understood. This study investigates the effects of exposure to sediment spiked with 1 μm polystyrene-based latex microplastic spheres, an environmentally realistic concentration of a pyrethroid pesticide (esfenvalerate), and a combination of both treatments on the life-history traits of the benthic-dwelling invertebrate, Chironomus riparius and its microbial community. The chironomid larvae were also exposed to two food conditions: abundant or limited food in the sediment, monitored for 28 and 34 days respectively. The microplastics and esfenvalerate had negative effects on adult emergence and survival, and these effects differed between the food level treatments. The microbiome diversity was negatively affected by the exposure to microplastics, while the relative abundances of the four top phyla were significantly affected only in the high food level treatment. Although the combined exposure to microplastics and esfenvalerate showed some negative effects on survival and emergence, there was little evidence for synergistic effects when compared to the single exposure. The food level affected all life-history traits and the microbiota, and lower food levels intensified the negative effects of the exposure to microplastics, esfenvalerate and their combination. We argue that these pollutants can affect crucial life-history traits such as successful metamorphosis and the host-microbiome. Therefore, it should be taken into consideration for toxicological assessment of pollutant acceptability. Our study highlights the importance of investigating possible additive and synergic activities between stressors to understand the effects of pollutants in the life story traits and host-microbiome.