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Immunocorrecting therapy of allergic dermatitis
2013
Omarbekova, G., Kazakh National Agrarian Univ., Almaty (Kazakhstan) | Muralinov, K., Kazakh National Agrarian Univ., Almaty (Kazakhstan) | Kozinda, O., Latvia Univ. of Agriculture, Jelgava (Latvia) | Makhmutov, A., Kazakh National Agrarian Univ., Almaty (Kazakhstan) | Muralinova, J., Kazakh National Agrarian Univ., Almaty (Kazakhstan
This article summarizes data of determining a therapeutic dose of a new immunocorrecting drug, dermatocytoglobulin, developed by the authors of this study. It has been established that this drug has a therapeutic immunocorrecting effect on allergic dermatitis. Subjects of the study cattle, horses, of Raimbek – Commercial Diary Farm, 56 dogs and 35 cats admitted to a veterinary clinic. It has been established that administration of dermatocytoglobulin in trial groups reduces autoantibody titers until their elimination on the 12th day, dose of 0.2 cm3 per 10 kg while autoantibody titers in control groups were identified on the 30th day.
Показать больше [+] Меньше [-]Detection of coronavirus among domestic animals
2024
Sadikaliyeva, Sandugash | Shorayeva, Kamshat | Abay, Zhandos | Jekebekov, Kuanish | Shayakhmetov, Yeraly
This article presents the results of studies on biological samples collected from 640 swabs taken from dogs and cats across various regions of the Republic of Kazakhstan. These samples were part of a monitoring study on the spread of coronavirus among domestic animals. Total RNAs were isolated using the magnetic sorption method with the ALPREP kit and subsequently analysed with the ALSENSE-SARS-CoV-2 RT-qPCR kit. Real-time RT-PCR analysis revealed that 104 samples tested positive within 22–37 amplification cycles. These positive samples were then cultured in Vero cell lines to confirm the presence of the virus. The biological activity of the resulting virus-containing suspension was determined using the Reed-Muench method. During cultivation, one viral isolate with a biological activity of 5.83±0.08 lg TCID50/ml was obtained. A microphotograph of the virus was taken using an electron microscope to determine its size, shape, and structure, which confirmed its morphology corresponding to the Coronaviridae family. The data obtained further indicate that domestic animals can suffer from and carry coronavirus. It is becoming increasingly evident that the virus can infect and replicate in the organs of various farm and domestic animals.
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