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Grain samples of 51 spring barley (Hordeum vulgare L.) genotypes, including two-row, six rows, covered and hull-less were used for investigation of endosperm beta-glucan content. A significant difference (p-value is less than 0.001) between genotypes in beta-glucan content was observed. On average, the six-row barleys had slightly higher beta-glucan content. Far six-row genotypes it ranged from 35.9 to 47.1 mg kgE-1 with the mean value of 41.4 mg kgE-1, but for tw0-row barleys - 38.0 mg kgE-1 within the range of 33.2-44.9 g kgE-1. Six-row genotypes had a higher coefficient of variation in comparison with two-row genotypes. Hull-less genotypes had significantly higher beta-glucan content than the covered ones (49.3 mg kgE-1 and 38.9 mg kgE-1 respectively). The standard deviation of the mean values of beta-glucan content for covered genotypes was lower than for the hull-less ones. The mean values in 2005 were well plotted against data from the previous season for genotypes included in the two years trial. There were two distinct clusters of covered and hull-less genotypes. Beta-glucan content for varieties bred in Latvia ranged from 33.2 mg kgE-1 for two-row variety 'Malva' to 46.0 mg kgE-1 for six-row variety 'Druvis'.

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