Parafasciolopsosis is a parasitological disease which is caused by the liver fluke Parafasciolopsis fasciolaemorpha. This parasite which belongs to herbivores often causes invasion in elks (Alces alces). The aim of our study was to diagnose the parasite and investigate what kind of histopathological changes it caused in the liver tissue. The study took place in Latvia University of Life Sciences and Technologies, Faculty of Veterinary Medicine, Laboratory of Comparative Pathology and Laboratory of Parasitology. During the study from 2017 to 2018 we collected liver samples and faeces from 46 felled elks of different age and gender, from all over Latvia. Ten grams of faeces were examined for trematode eggs by sedimentation method and a section of liver was examined for the presence of flukes and the histological structure of the liver. In this study Parafasciolopsis fasciolaemorpha were found in 11 samples of liver from 46 elks, with invasion extensity of 24%. The histopathological examination of the liver samples revealed fibrosis, proliferation of bile ducts, pericholangitis, calcerous deposits in bile ducts and other notable histological changes in the liver tissue that can cause hepatic trematodes.

Ruminants produce a large amount of methane (CH4 ) and carbon dioxide (CO2 ) in their foregut. These gases cause greenhouse effect. There are a lot of studies about different feed additives which can reduce the production of greenhouse gases in ruminants. Prebiotics can also change the amount of bacteria in animal gastrointestinal tract and reduce the occurrence of diarrhoea. The aim of this study was to test whether the prebiotic inulin affects the production of CH4 and CO2 in calves’ rumen and whether it affects the bacteria count in the rumen fluid and bacterial overgrowth in intestines. We used the flour of Jerusalem artichoke (Helianthus tuberosus L.) containing 50% of inulin. Approximately fifty days old, Holstein Friesian crossbreed calves were used in this study. Eight were in the control group, 8 received 12 g of flour and 8 received 24 g per day. On the 28th and 56th day of the research, we measured the amount of CH4 and CO2 in calves’ rumen took rumen fluid samples for bacterial analysis and urine to measure the level of phenol and indican. We concluded that adding the flour of Jerusalem artichoke at doses 12 g and 24 g did not significantly impact the production of CH4 and CO2 in calves’ rumen, the prebiotic inulin may suppress the growth of anaerobic microorganisms in the rumen at concentration 12 g of inulin reaching 56th day of experiment. The amount of phenol and indican in calves’ morning urine did not correlate with the faecal consistency of calves.

The aim of this study was to determine the influence of Jerusalem artichoke flour feeding on the general health status of calves (Bos Taurus), the animal live weight gain and digestive canal morphological development in the first four months of life, as well as to find out the optimal of three inulin doses. Jerusalem artichoke (Helianthus tuberosus) concentrate produced in Latvia contains the prebiotic – inulin. The study was performed on four groups of animals - a control group of 10 animals and three prebiotic groups, 10 animals in each group. All groups were fed the whole milk, but the PreG6 group animals were fed daily with 6 grams of prebiotic, calves PreG12 group were fed with 12g of prebiotic, but each calf in PreG24 group – 24g of prebiotic per day. CoG control animals did not receive feed supplement. Faecal mass consistence of PreG6, PreG12 groups of animals was more stable within the whole research period than CoG. We found that the control group animal weight gain on the 56th research day is statistically significantly (p is less than 0.05) lower than of those calves that received inulin as a food supplement. The highest growth rates have shown PreG12 and PreG24 group of animals, significantly (p is less than 0.05) being ahead of PreG6 group of calves, the highest average daily live weight gain was observed in PreG24 group (0.95 ± 0.093 kg).

The present work aimed to evaluate the effect of sea buckthorn (Hippophae rhamnoides) extract on Cryptosporidium spp. invasion in calves. Sea buckthorn is a good source of vitamins, carotenoids, organic acids and tannins. It has been reported that it possesses anti-inflammatory effect, as well as anthelmintic and antibacterial activity. Research was conducted in a dairy cattle farm ‘Mežacīruļi’ during April and July 2015. Experimental (n = 10) and control (n = 10) groups of calves were used. The experimental group received a mix of aqueous alcohol sea buckthorn leaf and berry pomace extract, administered with milk, at dose of 5 – 8 mL twice a day for 20 days. The control group received only milk. Samples were analysed with the concentration McMaster technique. The results showed that there is no significant (p is greater than 0.05) difference in the number of oocysts per gram of faeces between the experimental and control groups. It was concluded that the administration of sea buckthorn leaf and berry pomace extract had no effect on Cryptosporidium spp. invasion in calves. Studies will be continued with a higher dose of extract.