Scots pine (Pinus sylvestris) is Latvia's most economically important tree species. It accounts for 38% of the total forest area of Latvia. One of diseases affecting P. sylvestris is root rot caused by the fungus Heterobasidion annosum, which causes large economic losses. There is some evidence of Scots pine trees with higher levels of resistance but no absolutely resistant tree clones have been described so far. Many genes encoding peptides and proteins with direct or indirect antifungal activity have been described in various plant species, but only few of them have been studied in conifers. In our study we have utilised various approaches to research genetic aspects of Scots pine resistance to H. annosum. Here we present our initial results. Initially, H. annosum infection was determined in 300 trees and in a subset of twenty seven trees representing fifteen families (progeny of one mother tree) infection levels were quantitatively characterised. Candidate-genes were selected based on previously published research. Gene copy number variation (CNV) analyses were performed on selected samples. Copy number variation polymorphism was detected for a gene encoding a thaumatinlike protein analogues of which are described as potent antifungal proteins in other plants. As increased gene copy number can lead to increased gene product amounts in cells it is possible that an increased copy number of thaumatin-like protein is beneficial to the pine tree in respect to resistance against H. annosum and other pathogens. Further experiments need to be performed to investigate this in more detail.

Root rot caused by Heterobasidion spp. is one of the most important pathogens in Norway spruce (Picea abies (L.) Karst.) stands in Latvia. It is estimated that in Latvia on average 22.9% of Norway spruce stumps are infected with Heterobasidion spp. The aim of this study was to compare four different electronic devices for Heterobasidion root rot detection in Norway spruce. In the autumn of 2009, in a sample plot located in the forests of Kalsnava district 27 trees were used to compare the possibility of instruments to detect root rot. The results show that IML-RESI F400 accuracy for detecting root rot in Norway spruce is high and the instrument is usable for detecting root rot, but additional accumulators are needed for large sample plots. Rotfinder’s accuracy for detecting root rot varies from probe to probe taken from one tree. Conditiometer AS-1 and Arbo-Sonic decay detector show slightly higher average values for infected trees, but the difference for both instruments between healthy and decayed trees is not significant, thus leaving the usage of instruments questionable.

The biological control agent Rotstop, composed of spores of Phlebiopsis gigantea, is used for treatment of conifer stumps to reduce the spread of Heterobasidion root rot in commercial forests. Two experiments were conducted to compare the antagonistic ability of the Rotstop isolate and nine Latvian isolates of P. gigantea against root rot fungus Heterobasidion, in wood of Scots pine and Norway spruce. Billets of conifer trees were first sprayed with a spore suspension of P. gigantea and then with Heterobasidion at different concentrations. The presence of fungi in billets was evaluated by morphological characteristics of mycelium. The Latvian isolates of P. gigantea showed similar or even higher values of efficacy against Heterobasidion (average efficacy 84% in spruce and 89% in pine) than the commercially manufactured Finnish preparation Rotstop (61% in spruce and 90% in pine). Latvian isolates of P. gigantea have a potential to be used for preparation of biological control agents in the future.