In this study, it was aimed to reveal some viruses that are a problem in Dahlia plants grown for ornamental plants in parks and home gardens in Selçuklu, Karatay and Meram districts of Konya. For this reason, in the 2019 summer, leaf samples were taken from 152 Dahlia plants showing symptoms similar to virus disease symptoms such as mosaic, deformations, yellowing, blight on the leaves and stunted plants. Possible infections of Cucumber mosaic virus (CMV), Tomato spotted-wilt virus (TSWV) and Impatiens necrotic spot virus, (INSV) on these leaves were tested by serological-based DAS-ELISA (Double Antibody Sandwich- Enzyme Linked Immuno Sorbent Assay) method. According to DAS-ELISA results, it was determined that 46.71% of the samples were infected with at least one of these three viruses. In addition, TSWV was detected as the most common virus with a 38.82% relative incidence in the samples, followed by CMV (27.63%). Mixed infections of the viruses were also detected in the leaf samples tested. As a result of the tests, only CMV + TSWV double infection was detected in a total of 30 samples. On the other hand, INSV infection was not detected in any of the leaf samples tested. As a result of survey studies, the average of prevalence rate of virus diseases in Dahlia plants grown in Konya was calculated as 69.56%. Moreover, it was determined that virus diseases were most common in Selçuklu district (75.47%). This is the first report of CMV and TSWV on Dahlia spp. in Türkiye.

In this study, it was aimed to reveal some viruses that are a problem in tomato production areas in the Ereğli district of Konya, where tomato production is common. For this reason, in the 2019 production season, leaf samples were taken from 160 tomato plants showing symptoms similar to virus disease symptoms such as mosaic, deformations, yellowing, blight on the leaves and stunted plants. Possible infections of Cucumber mosaic virus (CMV), Tomato spotted-wilt virus (TSWV) and Tomato mosaic virus (ToMV) on these leaves were tested by serological-based DAS-ELISA (Double Antibody Sandwich- Enzyme Linked Immuno Sorbent Assay) method. According to DAS-ELISA results, it was determined that 66.25% of the samples were infected with at least one of these three viruses. In addition, TSWV was detected as the most common virus with a 48.75% relative infection rate in the samples, followed by ToMV (28.75%) and CMV (26.25%). Mixed infections of the viruses were also detected in the leaf samples tested. CMV + TSWV double infection was detected in 22 samples, ToMV + TSWV double infection was detected in 14 samples, and CMV + ToMV + TSWV triple infection was detected in 12 plant samples.

The escalating global population, diminishing agricultural lands, and the overarching global climate crisis are significantly impacting pepper cultivation. These challenges exacerbate the vulnerability of pepper plants to various biotic and abiotic factors, particularly viral diseases, resulting in diminished yield and quality. Cucumber mosaic virus (CMV), a notable concern for peppers in the Solanaceae family, is causing substantial quality and yield losses, with no effective chemical control methods currently available. This study focuses on exploring the genetic structure of CMV isolates obtained from pepper production areas in Kilis province and comparing these regions through the High-Resolution Melting (HRM) method. CMV isolates, cultivated in tobacco plants, have their partial coat protein sequences compared with those of other CMV isolates registered in the gene bank. The nucleotide sequences of identified CMV isolates are phylogenetically grouped and compared using an HRM graph. The HRM graph effectively distinguishes Kilis 3 and Kilis 4 isolates, clustered similarly to sequence patterns, from other isolates. The study highlights the utility of HRM analyses in identifying differences between isolates before determining sequence patterns. In the gene bank comparison, Kilis CMV isolates distinguished from others. Similarities were observed with isolates from Iran's Balsam (Impatiens balsamina - LC066478), Türkiye's Radish (Raphanus sativus - LC0665051), and Wild Turnip (Rapistrum rugosum - LC066514, LC066511, LC066517). The study found that Kilis 7 CMV isolate, transferred mechanically to 24 different pepper genotypes (C. annuum) from the local population, revealed susceptibility to CMV in the 24 lines developed from the Elbeyli population in Kilis province.