Fire blight disease caused by Erwinia amylovora can infect almost 140 plants of the Rosaceae family and poses a great threat to pome fruits growing all over the world. It needs amylovoran and Type III secretion systems (T3SS) to cause disease in host plants. AmsB, AmsD, AmsE, AmsF, AmsG, AmsJ, AmsI and AmsK proteins are involved in the binding of different galactose, glucuronic acid and pyruvyl subunits to the lipid carrier to form an amylovoran unit. T3SS proteins secreted by E. amylovora are HrpA HrpN, HrpW, AvrRpt2EA, HopC1 and DspA/E. DspA/E, the sole effector of E. amylovora, is secreted by during the formation of pilus T3SS. The chaperone protein of E. amylovora is DsB/F, which is in the IA class. EopB (outer membrane protein) has been characterized as one of the secretory proteins of E. amylovora. In addition to the harpins, the pathogenicity protein DspE and OrfB proteins are secreted via the Hrp-secretory system of E. amylovora. E. amylovora forms a Hrp pilus, which is produced by the structural protein HrpA. Genes encoding antimicrobial proteins cloned and expressed in apples and pears for impart resistance to the pathogen, attacin E are cecropins and lysozymes. The expression of PR2, PR5 and PR8 proteins is increased with E. amylovora infection in apple. Again, the HIPM protein in apples interacts with the E. amylovora HrpN protein, and the HIPM protein is found in higher amounts in flowers than leaves and shoots. In addition, four apple proteins (DIPMs) that interact with E. amylovora effector protein DspA/E have an effective role in endurance. In order to understand the interaction between the plant and the pathogen, it will be possible to understand the proteins that recognize the pathogen in the host, as well as the signal system and plant defense mechanism resulting from the infection. In this study, the roles of proteins associated with pathogenesis as a result of infection of E. amylovora in apples were tried to be revealed.

Fire blight disease caused by Erwinia amylovora is a destructive bacterial pathogen mainly on pears, apples and quinces from Rosaceae family. In this study, it was aimed determination of total protein amounts in different apple cultivars (Braeburn, Fuji, Gala and Golden), pear cultivars (Santa Maria and Williams) and quince cultivars (Eşme and Ekmek) in the infections of two virulent E. amylovora strains (Ea234-1 and Ea240-3) according as the time. It was taken leaf samples after leaf inoculation with E. amylovora (108 CFU ml-1) at 24th, 36th and 72nd hours. For verification of the infections, re-isolations were made from bacteria inoculated plants and the agent was identified as E. amylovora by biochemical, physiological and molecular tests. In determining the amounts of total protein and in the SDS-PAGE analyses were used Bradford and Laemmli methods, respectively, and absorbance values of protein extracts derived from the leaf samples taken, were obtained at 595 nm wavelength. According to the findings obtained; after infection of E. amylovora in the apple varieties comparing to controls, total protein concentrations at 24th hours increased and a decrease in the amount of 36th to 72nd hours and Braeburn has the highest protein content was determined. In the pear varieties, while total protein concentrations at 24th and 36th hours increased, a decrease in the amount of 72nd hour, and Santa Maria variety has the highest protein content was detected. In the quince varieties, total protein concentrations at 72th hour increased and Eşme variety has the highest protein content was identified. As a result of SDS-PAGE analysis, protein fractions which have different molecular weights were obtained. The protein bands were defined approximately 55-70 kDa and 35-55 kDa molecule weight on apple and quince varieties, respectively and also approx. 55-70 kDa in pear varieties.

The aim of the study was to design product profiles based on the factors and their levels impacting on the consumption preferences of the apples with Amasya, Isparta, Bayramic apples with the region of origin and foreign origin, and to determine the market shares of each profiles. The main material of the study was the primary data obtained from a face-to-face survey conducted with 600 households consuming apple and residing in the TRA1 region (Erzurum, Erzincan, Bayburt). Conjoint Analysis was used to design the apple profiles maximizing the consumers’ total utilities, and to determine the market share of each profile. Also, Cluster Analysis was applied to segment each apple profile and their market shares according to their income levels. The results of the study highlighted that low-income consumers attributed a great importance to the apple price (41%) and size (32%), but high-income ones to the region of origin (62%) and colour (13%) factors. On the other hand, while the apple profile with 2 number maximized total utility for both low-income group and all consumers, the profile 17 provided the highest satisfaction for high-income segment. The market shares of Amasya apple referred to the region of origin at the profile 2 and 17 for the low and high-income segments were analysed as 40% and 22%, 3% and 35%, respectively. Amasya apple according to other the region of origin maximised not only the consumers’ consumption satisfaction but also the market share at the supply chain.

Bu çalışmada şeker fabrikası atığı olan melas ve süt farikası yan ürünü olan peynir altı suyu elma ve portakal kaplamasında kullanılarak üç hafta boyunca depolanmıştır. Depolama süresince kalite değişimleri belirlenmiştir. Kaplamada kullanılan peynir altı suyu ve melasın briks değerleri sırasıyla 30 ve 50, pH’ları ise 1 ve 7.8’dir. Çalışmada kaplanan meyvelerin ağırlık kaybı ve renk değerleri incelenmiştir. Çalışma sonucunda üç haftalık depolama periyodunda elma için melas+su, portakal için ise peynir altı suyunun etkisinin önemli olduğu tespit edilmiştir. Melas+su ile kaplanan elmalarda ağırlık kaybı %3.645 iken kaplanmayan örneklerde %6.327’dir. Peynir altı suyu ile kaplanan portakallarda ağırlık kaybı %17.860 iken kaplanmayan örneklerde ise %27.930’dur. Çalışma ile melasın elmada, peynir altı suyunun da portakalda kaplama materyali olarak kullanılabileceği sonucuna varılmıştır. Özellikle ağırlık kaybı meyvelerin depolanmasında istenmeyen bir durumdur. Meyvelerin kaplanması ile bu durum minimuma indirilebilir. Kullanılan peynir altı suyu ve melas özellikle ağırlık kaybının azaltılmasında etkili olmuştur.