Length–weight, length–length relationships and condition factors were estimated for two fish species (Chondrostoma regium, Barbus lacerta) caught between October 2014 and September 2015 in the 14 distinct locations of Karasu River, East Anatolia, Turkey. Minimum and maximum total length and weight were observed at 7.5 to 31.0 cm and from 5.7 to 280 g for C. regium, 7.0 to 24.0 cm and from 4.0-211 g for B. lacerta. The b values determined as 2.77 for Chondrostoma regium; 3.10 for Barbus lacerta. The types of growth were found negative allometric for C. regium and isometric growth for B. lacerta. The length-length relations between total, fork, and standard lengths for the two fish species were highly significant. The relative condition factor values were found to 1.102±0.013 for C. regium; 1.176±0.019 for B. lacerta. This study submits for the first time LWR, LLR and condition factor of two species in the Karasu River.