High morbidity and mortality rate associated with pneumococcal infection globally is of major concern most especially among infant. This burden is equally worsened by multidrug resistance strains due to indiscriminate consumption of antibiotics. Hence, need for constant search for cheap and effective bioactive compounds as alternative antimicrobials for the treatment of pneumococcal infection. Bioactive compound profiling and in-vitro antibacterial activity of ginger methanol extract against two predominant pneumococcal agents; Streptococcus pneumonia and Haemophilus influenza were investigated. Gas Chromatography Mass Spectroscopy (GC-MS) was used for the identification and quantification of bioactive compounds in the ginger methanol extract. The antibacterial activity and Minimum Inhibitory Concentration (MIC) of the extract was determined using Agar well diffusion. Twenty-seven (27) matched bioactive compounds were detected in the sample. Zingerone (17.70%), α-zingiberene (13.30%), (6)-shogaol (10.84%), α-Farnesene (6.26%), β-Funebrene (5.61%), 6-gingerol (5.18%), α-curcumene (4.15%) were the major compounds present. All other identified compounds had less than 4% composition by peak area each. The antibacterial activity of the ginger crude methanol extract against S. pneumonia and H. influenza were 2.33 mm and 9.33 mm. MIC of the extract against the isolates was 10%. In conclusion ginger crude methanol extract contain an array of bioactive compounds and the extract exhibited antibacterial activity against predominant pneumococcal agents. Ginger extract can be harnessed for the production of new antimicrobials to combat pneumococcal infection.

Minerals have crucial roles in terms of vital function in the organism including hormones, enzymes, cell formation etc. Oxidative stress and related hormones, regarding infectious diseases, affect all functions of body including mineral taking, metabolism and excretion. The purpose of this study was to investigate the effect of the pox disease on blood minerals composition of sheep and lambs. This study was conducted on 20 healthy sheep, 20 poxvirus infected sheep and their lambs (Totally 80 subjects). One-shot blood samples from sheep and lambs were collected and serums were separated immediately. Serum mineral levels were analysed in the atomic absorption spectrophotometer. In general, the level of Cu in infected animals was higher than in healthy ones. Zn, Fe, Mg and Na levels of healthy animals were found higher than infected subjects. The difference between healthy and infected animal groups for serum Ca and K levels was not significant. Positive correlations were found between serum Zn, Fe, Mg and Na levels. However, a negative correlation was found between Cu-Zn and Cu-Fe pairs. Consequently, Cu, Zn, Fe, Mg and Na levels in serum, which are vital for animal organisms, showed significant changes in sheep and their lambs during poxvirus infection. To rapid healing or prevention of the diseases, mineral disorders must be corrected urgently.

Pseudomonas aeruginosa, belonging to the Pseudomonadaceae family, is Gram-negative, rod-shaped, motile, aerobic, endospore negative, oxidase and catalase positive. It is widely found in nature and isolated from soil, plants, water and animals. It can grow rapidly on the surface of the food and form oxidized products and mucous substances. P. aeruginosa, one of the leading foodborne pathogens, causes important concerns in food safety due to being a source of contamination, causing food poisoning and antimicrobial resistance in animals, forming biofilms and difficulties in preventing biofilms. In this review, information on history, microbiological, cultural and biochemical characteristics, virulence factors and pathogenicity of P. aeruginosa are given. In addition, infections caused by P. aeruginosa and its presence in food are described.