In this study, it was aimed to reveal the effect of temperature and humidity values provided by the semi-open and closed barns in the winter season on milk yield and components and the somatic cell count (SCC) level in milk in Anatolian buffaloes, and for this purpose, to compare both barn types and to reveal their suitability for buffalo breeding. In the study, temperature and humidity values were determined between December 2020 and March 2021 in two types of barns with similar feeding conditions in Bafra district, Samsun, and milk samples taken from buffaloes raised in these barns were analyzed. Temperature Humidity Index (THI) values were determined as 61,09±0,211 and 51.08±0.173 for the closed and semi-open barn type, respectively. In the study, in which the type of barn and therefore the temperature and humidity values provided in the barn environment did not affect the amount of milk yield, the milk fat ratio was determined as 8.89±0.227% and 8.16±0.176% for closed and semi-open barns, respectively. While other milk components were not affected by the barn conditions, the logSCC of milk was lower (4.54±0.071) in the closed barns when compared to the semi-open barns (4.73±0.044). Although no statistical difference was determined in the other milk parameters discussed, it was noted that there is an increasing tendency in the closed barns. It was observed that the changes in temperature and humidity values in both barn environments during the period caused nonlinear changes in milk composition. While indoor conditions were more effective on solids-non-fat (SNF), protein and lactose in the closed barn, the highest effect was on the fat ratio and logSCC in the semi-open barns. As a result, it can be said that both types of barns can be used in buffalo breeding since there is no significant difference in milk yield and other components, although there is a slight difference in milk fat ratio and logSCC level in closed and semi-open barns of Anatolian buffaloes in the climatic conditions of the region where the study was conducted.