In Afghanistan most times people are drinking olive leave tea for prevention and decline of higher lipid and glucose level, therefore the objective of the current study was to find out whether olive leaf extraction is having any effect on blood metabolites. The Japanese quail has been used for biological studies because of its easy management and large numbers in limited area. In this experiment quails (100 male and 100 Female) were reared on a farm owned by the Department of Animal science of Agriculture faculty of Nangrahar University. Quails were kept under optimum environmental during rearing period (20- 22 C and 65 % relative humidity). The Adaptation period was for 10 days. Feed was given add libitum during 24 h. In this study we had five treatments T0 served as control without any water supplementation, T1 (1g/L), T2 (2g/L), T3 (3g/L) and T4 (4g of olive leaf / litter of drinking water). The Experiment continued for 4 weeks at the end of week. By increasing level of the olive leave powder in water blood glucose, cholesterol and triglycerides levels declined significantly from 509 to 422.5, 204-144.5 and 245 to 192.5 respectively