Truffles are a highly valued fungi group because of their gastronomic and economic importance. Even though the demands for truffles, particularly Tuber melanosporum and T. aestivum, has been increased year by year, the natural production of these valued fungi has decreased. So, truffle cultivation is becoming an agricultural alternative in Europe, and the rural economy was supported by cultivating truffle. In Turkey, truffle cultivation was becoming popular in recent years. However, there is not enough literature for understanding the basics of truffle cultivation in Turkey. Hence, it is aimed to discuss the selection of suitable truffle land. Because land selection can be considered as the first step of truffle cultivation and unsuitable land can be limited truffle cultivation success. So, the ecological features of economically important truffles, T. aestivum, T. borchii, T. magnatum, and T. melanosporum, are given in this study based on current literature and field experience.

Tuber samples were collected from Kırklareli province on the 10th of August 2020 and they are identified by implementing both traditional methods and molecular phylogenetic analysis using the rDNA sequences including Internal Transcribed Spacer (ITS) and 28S Ribosomal Large Subunit (LSU) regions. By taking into account the high sequence similarity between the collected samples (ANK Akata 7351) and the truffle species Tuber fulgens Quél. the collected specimen was regarded as T. fulgens and the morphological data also consolidated this finding. As a result, T. fulgens was reported for the first time from Turkey. A short description of the newly reported species is given along with its macro and microphotographs, and spore images taken by a scanning electron microscope (SEM). Additionally, ITS and LSU rDNA based evolutionary history of the specimen is provided with phylogenetic trees.